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The map label for this gene is fdxH [H]

Identifier: 126208370

GI number: 126208370

Start: 1036582

End: 1037481

Strand: Direct

Name: fdxH [H]

Synonym: APL_0894

Alternate gene names: 126208370

Gene position: 1036582-1037481 (Clockwise)

Preceding gene: 126208369

Following gene: 126208371

Centisome position: 45.57

GC content: 46.22

Gene sequence:

>900_bases
ATGTCAGCAGTTCAAGACCAAAACATTATTAAAATCTCAGCAACTTCTTTTGTAACACCGCCACCGCAAGTGCGCGATCA
TGTTGTCGAAGTTGCAAAACTTATCGATGTTTCGACTTGTATCGGTTGTAAAGCCTGTCAAGTGGCTTGTTCGGAATGGA
ATGATCTTCGTGCGCCGCAAGAAGAATGCGTAGGGGTTTACGATAACCCTCGTGACTTAAATGCCGAACAATGGACGGTG
ATGAAGTTCAACGAAGTGGAAGAAAACGACCGCTTGGAATGGTTAATTCGCAAAGACGGTTGTATGCACTGTGCGGAACC
GGGCTGCTTAAAAGCATGTCCGGCACCGGGTGCAATCATTCAATATGCTAACGGTATCGTGGACTTCCAATCCGATAAAT
GTATCGGTTGCGGTTACTGTATTGCGGGTTGTCCGTTCAACATTCCTCGTATGAATGACAACGATAACCGTGTGTACAAA
TGTACCCTTTGCGTGGATCGTGTGAACGTAGGTCAAGAACCGGCATGTGTGAAAACCTGTCCGACAGGTGCTATTCGTTT
CGGTTCTAAAGAAGAAATGCTTCACTACGGTGAACAACGTGTTGCGGATCTGAAAAGTCGCGGCTACGAAAATGCCGGTA
TTTATAACCCGGAAGGTGTGGGCGGTACGCACGTAATGTATGTATTGCACCATGCGGATAAACCTGAATTATATTCCGGT
TTACCGAAAGATCCGAGTATCGACCCGACAGTTACACTTTGGAAAGACGTCTTGAAACCGGTTGCGGCTATTGCAATGGG
CGGCTTGGCACTTGCTGAAGTTGCACACTATGTAACCGTTGGTCCGAACATTGAAGAAGATGTTGAAGATCACCATGAGG
AAGGAGAAAAACATGAGTAA

Upstream 100 bases:

>100_bases
TTGCCAATAGACTTACAGGTCGTGTCGGTGACGGTAATACTTTAACGCCTGAATATAAAACGTTCCTTGTGAACATTGAG
AAAGTTTCGGAGGTGGCATA

Downstream 100 bases:

>100_bases
GAAATTTGAAATTACGAACGATACCAAAATCGTTCGTCATAAACCGCTTGCCCGTGTCAGCCACTGGTTCTTGGTTATCT
CGTTCTTCTTAACTATGTTT

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH2 [C]

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSAVQDQNIIKISATSFVTPPPQVRDHVVEVAKLIDVSTCIGCKACQVACSEWNDLRAPQEECVGVYDNPRDLNAEQWTV
MKFNEVEENDRLEWLIRKDGCMHCAEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNDNDNRVYK
CTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMLHYGEQRVADLKSRGYENAGIYNPEGVGGTHVMYVLHHADKPELYSG
LPKDPSIDPTVTLWKDVLKPVAAIAMGGLALAEVAHYVTVGPNIEEDVEDHHEEGEKHE

Sequences:

>Translated_299_residues
MSAVQDQNIIKISATSFVTPPPQVRDHVVEVAKLIDVSTCIGCKACQVACSEWNDLRAPQEECVGVYDNPRDLNAEQWTV
MKFNEVEENDRLEWLIRKDGCMHCAEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNDNDNRVYK
CTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMLHYGEQRVADLKSRGYENAGIYNPEGVGGTHVMYVLHHADKPELYSG
LPKDPSIDPTVTLWKDVLKPVAAIAMGGLALAEVAHYVTVGPNIEEDVEDHHEEGEKHE
>Mature_298_residues
SAVQDQNIIKISATSFVTPPPQVRDHVVEVAKLIDVSTCIGCKACQVACSEWNDLRAPQEECVGVYDNPRDLNAEQWTVM
KFNEVEENDRLEWLIRKDGCMHCAEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNDNDNRVYKC
TLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMLHYGEQRVADLKSRGYENAGIYNPEGVGGTHVMYVLHHADKPELYSGL
PKDPSIDPTVTLWKDVLKPVAAIAMGGLALAEVAHYVTVGPNIEEDVEDHHEEGEKHE

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=73.3788395904437, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790326, Length=296, Percent_Identity=69.2567567567568, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789370, Length=305, Percent_Identity=29.8360655737705, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=33.7349397590361, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=33.7349397590361, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI2367345, Length=158, Percent_Identity=31.6455696202532, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=27.6243093922652, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787740, Length=136, Percent_Identity=32.3529411764706, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=36, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082180, Length=157, Percent_Identity=28.6624203821656, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: NA

Molecular weight: Translated: 32921; Mature: 32789

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVQDQNIIKISATSFVTPPPQVRDHVVEVAKLIDVSTCIGCKACQVACSEWNDLRAPQ
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCH
EECVGVYDNPRDLNAEQWTVMKFNEVEENDRLEWLIRKDGCMHCAEPGCLKACPAPGAII
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
QYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNDNDNRVYKCTLCVDRVNVGQEPACVKTC
HHHCCEEEECCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEC
PTGAIRFGSKEEMLHYGEQRVADLKSRGYENAGIYNPEGVGGTHVMYVLHHADKPELYSG
CCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCC
LPKDPSIDPTVTLWKDVLKPVAAIAMGGLALAEVAHYVTVGPNIEEDVEDHHEEGEKHE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAVQDQNIIKISATSFVTPPPQVRDHVVEVAKLIDVSTCIGCKACQVACSEWNDLRAPQ
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCH
EECVGVYDNPRDLNAEQWTVMKFNEVEENDRLEWLIRKDGCMHCAEPGCLKACPAPGAII
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
QYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNDNDNRVYKCTLCVDRVNVGQEPACVKTC
HHHCCEEEECCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEC
PTGAIRFGSKEEMLHYGEQRVADLKSRGYENAGIYNPEGVGGTHVMYVLHHADKPELYSG
CCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCC
LPKDPSIDPTVTLWKDVLKPVAAIAMGGLALAEVAHYVTVGPNIEEDVEDHHEEGEKHE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases; Acting on the aldehyde or oxo group of donors [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]