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The map label for this gene is fdnI [H]

Identifier: 126208371

GI number: 126208371

Start: 1037474

End: 1038145

Strand: Direct

Name: fdnI [H]

Synonym: APL_0895

Alternate gene names: 126208371

Gene position: 1037474-1038145 (Clockwise)

Preceding gene: 126208370

Following gene: 126208372

Centisome position: 45.61

GC content: 41.52

Gene sequence:

>672_bases
ATGAGTAAGAAATTTGAAATTACGAACGATACCAAAATCGTTCGTCATAAACCGCTTGCCCGTGTCAGCCACTGGTTCTT
GGTTATCTCGTTCTTCTTAACTATGTTTACCGGTGTGGCGTTTTTCTTCCCGGATTTCGCATGGCTAACCGAGATCTTAG
GTACGCCGCAACTTGCGCGAGCGATCCATCCGTTTACCGGTATTGTGATGTTTGTCGCCTTTATTATTATGGCGATCATT
TACTGGCATCATAATATCCCGGAGAAAAACGATATTCGCTGGGCAAAAGGGATTGTTGAAGTATTAAAAGGTAACGAACA
CGCCGTTGCTTATAACGGTAAATATAACCTCGGTCAGAAAATGTTGTTCTGGACCTTGATTTTGGCAATGTTCACCCTTT
TAATTACGGGCATTATCATGTGGAGAAAATATTTCTCCGATAACTTCTCAATTCAAACTTTACGTATTGCGATCTTGCTT
CACTCTGCTAGTGCCTTCGCATTGTTTACCGGTATTTTGGTACATATGTATATGGCATTTTGGGTGAAAGGTTCTATCCG
CGGTATGGTTGAAGGTTGGGTAACCGTTCGTTGGGCGAAAAAACACCATCCGAAATGGCTTAAAGAAGAAGTATTACCCG
AGCTTGAAAAACAAGCGAACAGAAATAACTAA

Upstream 100 bases:

>100_bases
GCAATGGGCGGCTTGGCACTTGCTGAAGTTGCACACTATGTAACCGTTGGTCCGAACATTGAAGAAGATGTTGAAGATCA
CCATGAGGAAGGAGAAAAAC

Downstream 100 bases:

>100_bases
TTCTTTTTAAAGATACAAGTGCGGTAAACTTACCGCACTTTATTTTTAAATACTTGCTAAATTTCTTTTGACTAAGGGAA
TTTAGCAAGATTTAACGAGA

Product: formate dehydrogenase, cytochrome b556 subunit

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit gamma; FDH subunit gamma [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSKKFEITNDTKIVRHKPLARVSHWFLVISFFLTMFTGVAFFFPDFAWLTEILGTPQLARAIHPFTGIVMFVAFIIMAII
YWHHNIPEKNDIRWAKGIVEVLKGNEHAVAYNGKYNLGQKMLFWTLILAMFTLLITGIIMWRKYFSDNFSIQTLRIAILL
HSASAFALFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPKWLKEEVLPELEKQANRNN

Sequences:

>Translated_223_residues
MSKKFEITNDTKIVRHKPLARVSHWFLVISFFLTMFTGVAFFFPDFAWLTEILGTPQLARAIHPFTGIVMFVAFIIMAII
YWHHNIPEKNDIRWAKGIVEVLKGNEHAVAYNGKYNLGQKMLFWTLILAMFTLLITGIIMWRKYFSDNFSIQTLRIAILL
HSASAFALFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPKWLKEEVLPELEKQANRNN
>Mature_222_residues
SKKFEITNDTKIVRHKPLARVSHWFLVISFFLTMFTGVAFFFPDFAWLTEILGTPQLARAIHPFTGIVMFVAFIIMAIIY
WHHNIPEKNDIRWAKGIVEVLKGNEHAVAYNGKYNLGQKMLFWTLILAMFTLLITGIIMWRKYFSDNFSIQTLRIAILLH
SASAFALFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPKWLKEEVLPELEKQANRNN

Specific function: Allows to use formate as major electron donor during anaerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=216, Percent_Identity=52.3148148148148, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI1790325, Length=212, Percent_Identity=50.4716981132076, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 26045; Mature: 25914

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKFEITNDTKIVRHKPLARVSHWFLVISFFLTMFTGVAFFFPDFAWLTEILGTPQLAR
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
AIHPFTGIVMFVAFIIMAIIYWHHNIPEKNDIRWAKGIVEVLKGNEHAVAYNGKYNLGQK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHH
MLFWTLILAMFTLLITGIIMWRKYFSDNFSIQTLRIAILLHSASAFALFTGILVHMYMAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVKGSIRGMVEGWVTVRWAKKHHPKWLKEEVLPELEKQANRNN
HHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKKFEITNDTKIVRHKPLARVSHWFLVISFFLTMFTGVAFFFPDFAWLTEILGTPQLAR
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
AIHPFTGIVMFVAFIIMAIIYWHHNIPEKNDIRWAKGIVEVLKGNEHAVAYNGKYNLGQK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHH
MLFWTLILAMFTLLITGIIMWRKYFSDNFSIQTLRIAILLHSASAFALFTGILVHMYMAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVKGSIRGMVEGWVTVRWAKKHHPKWLKEEVLPELEKQANRNN
HHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]