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The map label for this gene is fdxG [H]

Identifier: 126208369

GI number: 126208369

Start: 1034159

End: 1036582

Strand: Direct

Name: fdxG [H]

Synonym: APL_0893

Alternate gene names: 126208369

Gene position: 1034159-1036582 (Clockwise)

Preceding gene: 126208368

Following gene: 126208370

Centisome position: 45.47

GC content: 43.52

Gene sequence:

>2424_bases
ATGACAAACCACTGGGTGGATATCAAAAATGCGGACGTAGTGGTAGTAATGGGCGGTAATGCCGCTGAAGCTCACCCTGT
CGGTTTCCGCTGGGCAATCGAAGCGAAAAAACAAAACGGTGCGAAGTTAATGGTGGTTGATCCGCGCTTTAACCGTACGG
CATCCGTTGCGGATTTCTATTCTCCGATTCGTTCCGGTACGGATATTACGTTCTTATCAGGCGTAATCAAATACCTACTC
GATAACGATAAAATTCAACACGAATACGTTAAACATTACACGAACGCAAGTTTCTTAATTAACGAAGGTTTTGGTTTTGA
AGACGGTTTATTTACCGGTTACGACGAAGTAAAACGTAGCTACGATAAATCGACTTGGACTTATCAGCTTGATGAAAACG
GTCAGCCGAAACGTGATATGACGTTACAAGATCCGCGTTGTGTTATCAATTTATTGAAAAAACACGTTGAACGTTATACA
CCGGAAATGGTGGAACGTATTTGCGGTACCAAACAAGACAAATTCTTAGAATTTGCTCAAACCATCGCATCAACGGCTAC
ACCGGAAAGAACAACGACATTCTTATATGCGTTAGGTTGGACACAACATACGATTGGTTCGCAAAATATTCGTACCATGG
CGATCATCCAATTGCTATTAGGTAATATCGGTATGGCAGGCGGCGGCGTAAACGCATTACGCGGTCACTCGAACGTACAA
GGTACGACTGATATGGGCTTATTCCCTCATATGTTACCGGGTTATATTCCGTTACCGACAGAATCGGATAAAACGCTTGA
ATCGTTCTTAAATCGTATTACGCCGAAAACAGCGGTGGAAGGTCAGGTAAACTACTGGCAAAACACCCCGAAATTTATGG
TGAGTATGCTGAAATCGTTCTACGGCGATAAAGCGACCAAAGACAACGAATTCGGTTATCACTATTTACCGAAACAGTTG
AAATACCGTTTAGACCATCTTCGTTTTATTGACATGATGGATGAAGGTAAAGTAACCGGTTATCTCTGCCAAGGTTATAA
CCCGTTAGCGTCTTACCCTGACTCAAACAAAATTTCAAGTGCATTACGTAAATTAAAATTCTTAGTCATTATGGATCCGT
TAGCGACCGATACTTCGGAGTTCTGGAAAAACCATGGTGAATTAAATGATGTGAACCCGGCGGAAATTCAAACCGAAGTA
TTCCGTTTACCAAGTATTTGTTTTGCGGAAGAAGACGGTTCGATTGCAAACTCAGGTCGTTGGCTACAATGGCACTGGAA
AGCGACCGAACCACCGAAAGAAGCAAAACCGGATGTAGATATCCTTGCCGAAATCCGTGAAGTGATGATGGAAATGTATC
ACAAAGAAGGCGGTAAAGGTTTAGAAAGCTTTGAAGCGATGAGCTGGAATTATGCGAATCCGCTCGAGCCGAAAGCGGAC
GAAATTGCGAAAGAAAACAACGGTTACGCATTAGAAGATCTTTATGATGCTAACGGTAACTTAGTTGCGAAAAAAGGCGA
TTTACTTTCAAGTTTCGCCTTACTTCGTAATGACGGTTCGACCTCTTCCGCTTGCTGGATTTATACCGGTCAATGGACGC
AAAAAGGTAACCAAATGGCAAACCGTGATAACTCCGACCCTTCAGGTCTAGGTAATACGCTAGGTTGGTCGTTTGCATGG
CCTGCTAACCGTCGTGTTCTCTATAACAGAGCATCAGCGGACTTAACGGGTAAACCTTGGAACCCTAAACGTCAGTTAAT
TAAATGGAACAGTAAAAACTGGAACTATATTGATGTAGCGGACTTCGGTACTGCGGCACCGAATACTTCCGTATCACCAT
TTATCATGACAAATGACGGTGTTTCGCATTTATTCGCACCGCAATTGGCTGAAGGTCCGTTCCCAGAACACTATGAACCG
ATTGAAACGCCGATTGGTACTAACCCGTTACACCCGAATGTGGTTTCAAACCCGGTTGCACGTATTTTACCGCGTGATAA
AGATCGCTTCGGTACGGCGGACGAGTTCCCATTCGTAGGAACAACCTATCGTTTAACCGAGCATTTCCACTGGTGGACTA
AACACTCGCAATTAAACATGATTACACAGCCTGAGCCGTTCGTAGAAATCAGCGAACAACTTGCCGCGCTCAAAGGTATC
AGTCATGGCGATACGGTAAAAATTACTTCAAAACGCGGTTATATTAAAACTAAAGTTGTAGTAACTAAACGTATTAAACC
GCTTATGGCGGACGGAAAAGAAATCCATACGGTCGGTATCCCGATTCACGGCGGATTTAAATCGGCGGGTAATAAAGGTC
ACCTTGCCAATAGACTTACAGGTCGTGTCGGTGACGGTAATACTTTAACGCCTGAATATAAAACGTTCCTTGTGAACATT
GAGAAAGTTTCGGAGGTGGCATAA

Upstream 100 bases:

>100_bases
GTGGGCTCGTGCGCTCGGTTTAGTTGTAATCGACCAAGTAGCGAGTACCTGACACGGACCAACGGTAGCAAGTCTTGCTC
CAACATTTGGTCGCGGTGCC

Downstream 100 bases:

>100_bases
TGTCAGCAGTTCAAGACCAAAACATTATTAAAATCTCAGCAACTTCTTTTGTAACACCGCCACCGCAAGTGCGCGATCAT
GTTGTCGAAGTTGCAAAACT

Product: formate dehydrogenase, nitrate-inducible, major subunit

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MTNHWVDIKNADVVVVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTASVADFYSPIRSGTDITFLSGVIKYLL
DNDKIQHEYVKHYTNASFLINEGFGFEDGLFTGYDEVKRSYDKSTWTYQLDENGQPKRDMTLQDPRCVINLLKKHVERYT
PEMVERICGTKQDKFLEFAQTIASTATPERTTTFLYALGWTQHTIGSQNIRTMAIIQLLLGNIGMAGGGVNALRGHSNVQ
GTTDMGLFPHMLPGYIPLPTESDKTLESFLNRITPKTAVEGQVNYWQNTPKFMVSMLKSFYGDKATKDNEFGYHYLPKQL
KYRLDHLRFIDMMDEGKVTGYLCQGYNPLASYPDSNKISSALRKLKFLVIMDPLATDTSEFWKNHGELNDVNPAEIQTEV
FRLPSICFAEEDGSIANSGRWLQWHWKATEPPKEAKPDVDILAEIREVMMEMYHKEGGKGLESFEAMSWNYANPLEPKAD
EIAKENNGYALEDLYDANGNLVAKKGDLLSSFALLRNDGSTSSACWIYTGQWTQKGNQMANRDNSDPSGLGNTLGWSFAW
PANRRVLYNRASADLTGKPWNPKRQLIKWNSKNWNYIDVADFGTAAPNTSVSPFIMTNDGVSHLFAPQLAEGPFPEHYEP
IETPIGTNPLHPNVVSNPVARILPRDKDRFGTADEFPFVGTTYRLTEHFHWWTKHSQLNMITQPEPFVEISEQLAALKGI
SHGDTVKITSKRGYIKTKVVVTKRIKPLMADGKEIHTVGIPIHGGFKSAGNKGHLANRLTGRVGDGNTLTPEYKTFLVNI
EKVSEVA

Sequences:

>Translated_807_residues
MTNHWVDIKNADVVVVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTASVADFYSPIRSGTDITFLSGVIKYLL
DNDKIQHEYVKHYTNASFLINEGFGFEDGLFTGYDEVKRSYDKSTWTYQLDENGQPKRDMTLQDPRCVINLLKKHVERYT
PEMVERICGTKQDKFLEFAQTIASTATPERTTTFLYALGWTQHTIGSQNIRTMAIIQLLLGNIGMAGGGVNALRGHSNVQ
GTTDMGLFPHMLPGYIPLPTESDKTLESFLNRITPKTAVEGQVNYWQNTPKFMVSMLKSFYGDKATKDNEFGYHYLPKQL
KYRLDHLRFIDMMDEGKVTGYLCQGYNPLASYPDSNKISSALRKLKFLVIMDPLATDTSEFWKNHGELNDVNPAEIQTEV
FRLPSICFAEEDGSIANSGRWLQWHWKATEPPKEAKPDVDILAEIREVMMEMYHKEGGKGLESFEAMSWNYANPLEPKAD
EIAKENNGYALEDLYDANGNLVAKKGDLLSSFALLRNDGSTSSACWIYTGQWTQKGNQMANRDNSDPSGLGNTLGWSFAW
PANRRVLYNRASADLTGKPWNPKRQLIKWNSKNWNYIDVADFGTAAPNTSVSPFIMTNDGVSHLFAPQLAEGPFPEHYEP
IETPIGTNPLHPNVVSNPVARILPRDKDRFGTADEFPFVGTTYRLTEHFHWWTKHSQLNMITQPEPFVEISEQLAALKGI
SHGDTVKITSKRGYIKTKVVVTKRIKPLMADGKEIHTVGIPIHGGFKSAGNKGHLANRLTGRVGDGNTLTPEYKTFLVNI
EKVSEVA
>Mature_806_residues
TNHWVDIKNADVVVVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTASVADFYSPIRSGTDITFLSGVIKYLLD
NDKIQHEYVKHYTNASFLINEGFGFEDGLFTGYDEVKRSYDKSTWTYQLDENGQPKRDMTLQDPRCVINLLKKHVERYTP
EMVERICGTKQDKFLEFAQTIASTATPERTTTFLYALGWTQHTIGSQNIRTMAIIQLLLGNIGMAGGGVNALRGHSNVQG
TTDMGLFPHMLPGYIPLPTESDKTLESFLNRITPKTAVEGQVNYWQNTPKFMVSMLKSFYGDKATKDNEFGYHYLPKQLK
YRLDHLRFIDMMDEGKVTGYLCQGYNPLASYPDSNKISSALRKLKFLVIMDPLATDTSEFWKNHGELNDVNPAEIQTEVF
RLPSICFAEEDGSIANSGRWLQWHWKATEPPKEAKPDVDILAEIREVMMEMYHKEGGKGLESFEAMSWNYANPLEPKADE
IAKENNGYALEDLYDANGNLVAKKGDLLSSFALLRNDGSTSSACWIYTGQWTQKGNQMANRDNSDPSGLGNTLGWSFAWP
ANRRVLYNRASADLTGKPWNPKRQLIKWNSKNWNYIDVADFGTAAPNTSVSPFIMTNDGVSHLFAPQLAEGPFPEHYEPI
ETPIGTNPLHPNVVSNPVARILPRDKDRFGTADEFPFVGTTYRLTEHFHWWTKHSQLNMITQPEPFVEISEQLAALKGIS
HGDTVKITSKRGYIKTKVVVTKRIKPLMADGKEIHTVGIPIHGGFKSAGNKGHLANRLTGRVGDGNTLTPEYKTFLVNIE
KVSEVA

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=804, Percent_Identity=68.1592039800995, Blast_Score=1179, Evalue=0.0,
Organism=Escherichia coli, GI3868720, Length=805, Percent_Identity=63.8509316770186, Blast_Score=1101, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=449, Percent_Identity=24.7216035634744, Blast_Score=127, Evalue=4e-30,
Organism=Escherichia coli, GI1787778, Length=259, Percent_Identity=26.6409266409266, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081797, Length=235, Percent_Identity=25.1063829787234, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI171474008, Length=251, Percent_Identity=24.7011952191235, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787870, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 90738; Mature: 90606

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHWVDIKNADVVVVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTASVADFY
CCCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
SPIRSGTDITFLSGVIKYLLDNDKIQHEYVKHYTNASFLINEGFGFEDGLFTGYDEVKRS
HHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHH
YDKSTWTYQLDENGQPKRDMTLQDPRCVINLLKKHVERYTPEMVERICGTKQDKFLEFAQ
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
TIASTATPERTTTFLYALGWTQHTIGSQNIRTMAIIQLLLGNIGMAGGGVNALRGHSNVQ
HHHHCCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
GTTDMGLFPHMLPGYIPLPTESDKTLESFLNRITPKTAVEGQVNYWQNTPKFMVSMLKSF
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHH
YGDKATKDNEFGYHYLPKQLKYRLDHLRFIDMMDEGKVTGYLCQGYNPLASYPDSNKISS
HCCCCCCCCCCCCHHCCHHHHHHHHHHHEEHHHCCCCEEEEEECCCCHHHCCCCCHHHHH
ALRKLKFLVIMDPLATDTSEFWKNHGELNDVNPAEIQTEVFRLPSICFAEEDGSIANSGR
HHHHHEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCC
WLQWHWKATEPPKEAKPDVDILAEIREVMMEMYHKEGGKGLESFEAMSWNYANPLEPKAD
EEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHH
EIAKENNGYALEDLYDANGNLVAKKGDLLSSFALLRNDGSTSSACWIYTGQWTQKGNQMA
HHHHCCCCEEEHEEECCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCHHH
NRDNSDPSGLGNTLGWSFAWPANRRVLYNRASADLTGKPWNPKRQLIKWNSKNWNYIDVA
CCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHEEECCCCCCEEEEC
DFGTAAPNTSVSPFIMTNDGVSHLFAPQLAEGPFPEHYEPIETPIGTNPLHPNVVSNPVA
CCCCCCCCCCCCCEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
RILPRDKDRFGTADEFPFVGTTYRLTEHFHWWTKHSQLNMITQPEPFVEISEQLAALKGI
HHCCCCCCCCCCCCCCCCCCEEEEHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHHHCC
SHGDTVKITSKRGYIKTKVVVTKRIKPLMADGKEIHTVGIPIHGGFKSAGNKGHLANRLT
CCCCEEEEECCCCEEEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHC
GRVGDGNTLTPEYKTFLVNIEKVSEVA
CCCCCCCCCCCCCEEEEEEHHHHHCCC
>Mature Secondary Structure 
TNHWVDIKNADVVVVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTASVADFY
CCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCHHHHHHH
SPIRSGTDITFLSGVIKYLLDNDKIQHEYVKHYTNASFLINEGFGFEDGLFTGYDEVKRS
HHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHH
YDKSTWTYQLDENGQPKRDMTLQDPRCVINLLKKHVERYTPEMVERICGTKQDKFLEFAQ
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
TIASTATPERTTTFLYALGWTQHTIGSQNIRTMAIIQLLLGNIGMAGGGVNALRGHSNVQ
HHHHCCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
GTTDMGLFPHMLPGYIPLPTESDKTLESFLNRITPKTAVEGQVNYWQNTPKFMVSMLKSF
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHH
YGDKATKDNEFGYHYLPKQLKYRLDHLRFIDMMDEGKVTGYLCQGYNPLASYPDSNKISS
HCCCCCCCCCCCCHHCCHHHHHHHHHHHEEHHHCCCCEEEEEECCCCHHHCCCCCHHHHH
ALRKLKFLVIMDPLATDTSEFWKNHGELNDVNPAEIQTEVFRLPSICFAEEDGSIANSGR
HHHHHEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCC
WLQWHWKATEPPKEAKPDVDILAEIREVMMEMYHKEGGKGLESFEAMSWNYANPLEPKAD
EEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHH
EIAKENNGYALEDLYDANGNLVAKKGDLLSSFALLRNDGSTSSACWIYTGQWTQKGNQMA
HHHHCCCCEEEHEEECCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCHHH
NRDNSDPSGLGNTLGWSFAWPANRRVLYNRASADLTGKPWNPKRQLIKWNSKNWNYIDVA
CCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHHEEECCCCCCEEEEC
DFGTAAPNTSVSPFIMTNDGVSHLFAPQLAEGPFPEHYEPIETPIGTNPLHPNVVSNPVA
CCCCCCCCCCCCCEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
RILPRDKDRFGTADEFPFVGTTYRLTEHFHWWTKHSQLNMITQPEPFVEISEQLAALKGI
HHCCCCCCCCCCCCCCCCCCEEEEHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHHHCC
SHGDTVKITSKRGYIKTKVVVTKRIKPLMADGKEIHTVGIPIHGGFKSAGNKGHLANRLT
CCCCEEEEECCCCEEEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHC
GRVGDGNTLTPEYKTFLVNIEKVSEVA
CCCCCCCCCCCCCEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]