Click here to switch to the map view.

The map label for this gene is fdxG [H]

Identifier: 126208368

GI number: 126208368

Start: 1033523

End: 1034110

Strand: Direct

Name: fdxG [H]

Synonym: APL_0892

Alternate gene names: 126208368

Gene position: 1033523-1034110 (Clockwise)

Preceding gene: 126208365

Following gene: 126208369

Centisome position: 45.44

GC content: 49.32

Gene sequence:

>588_bases
ATGCAGATCTCAAGACGTAAGTTCTTTAAGGTCTGTGCAGGTGGTATGGCGGGTACTTCTGCGGCGATGTTGGGCTTTGC
CCCGGCAACCGCATTGGCAGCCCCGCGCGAATATAAACTTTTGCGTGCAAAAGAAACTCGCCAAACCTGTACATACTGTT
CTGTCGGCTGTGGTATGTTGATGTATAGCCTCGGCGACGGTGCAATGAATTCAAGAGGAAAACTTTTCCATGTGGAAGGC
GATCCTGACCATCCGGTAAGTCGCGGCGCACTTTGTCCTAAAGGCGCAGGCGTATTAGACTATGTAAACAGTCCGAATCG
TTTACAATATCCTGAATACCGCGCGCCGGGTTCGGATAAATGGGAACGAATTTCTTGGCACGACGCGATTCATCGTATTG
CAAAATTAATGAAAGAAGACCGCGATGCGAACTGGCAAGATGTCAATGAAGAAGGAACTGTCGTCAATCGTTGGACCACC
ACCGGTTTACTTTGCGGTTCCGCAACAAGTAACGAAACCTCTCTTGTTTCCCAAAAGTGGGCTCGTGCGCTCGGTTTAGT
TGTAATCGACCAAGTAGCGAGTACCTGA

Upstream 100 bases:

>100_bases
AAGGTAAATAATAGCCTTTACTTTAAATGTATTGCGATGCAGTTAAAGTGTTGGTTATTTACATTAATTTGTGGTTAATG
TTCTATTTTTAGGAGTGATT

Downstream 100 bases:

>100_bases
CACGGACCAACGGTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCCATGACAAACCACTGGGTGGATATCAAAAATGC
GGACGTAGTGGTAGTAATGG

Product: formate dehydrogenase, nitrate-inducible, major subunit

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAKETRQTCTYCSVGCGMLMYSLGDGAMNSRGKLFHVEG
DPDHPVSRGALCPKGAGVLDYVNSPNRLQYPEYRAPGSDKWERISWHDAIHRIAKLMKEDRDANWQDVNEEGTVVNRWTT
TGLLCGSATSNETSLVSQKWARALGLVVIDQVAST

Sequences:

>Translated_195_residues
MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAKETRQTCTYCSVGCGMLMYSLGDGAMNSRGKLFHVEG
DPDHPVSRGALCPKGAGVLDYVNSPNRLQYPEYRAPGSDKWERISWHDAIHRIAKLMKEDRDANWQDVNEEGTVVNRWTT
TGLLCGSATSNETSLVSQKWARALGLVVIDQVAST
>Mature_195_residues
MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAKETRQTCTYCSVGCGMLMYSLGDGAMNSRGKLFHVEG
DPDHPVSRGALCPKGAGVLDYVNSPNRLQYPEYRAPGSDKWERISWHDAIHRIAKLMKEDRDANWQDVNEEGTVVNRWTT
TGLLCGSATSNETSLVSQKWARALGLVVIDQVAST

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=193, Percent_Identity=70.9844559585492, Blast_Score=306, Evalue=7e-85,
Organism=Escherichia coli, GI3868720, Length=193, Percent_Identity=68.3937823834197, Blast_Score=301, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 21346; Mature: 21346

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAKETRQTCTYCSVGCGML
CCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MYSLGDGAMNSRGKLFHVEGDPDHPVSRGALCPKGAGVLDYVNSPNRLQYPEYRAPGSDK
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
WERISWHDAIHRIAKLMKEDRDANWQDVNEEGTVVNRWTTTGLLCGSATSNETSLVSQKW
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEEEECCCCCCHHHHHHHHH
ARALGLVVIDQVAST
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAKETRQTCTYCSVGCGML
CCCHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MYSLGDGAMNSRGKLFHVEGDPDHPVSRGALCPKGAGVLDYVNSPNRLQYPEYRAPGSDK
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
WERISWHDAIHRIAKLMKEDRDANWQDVNEEGTVVNRWTTTGLLCGSATSNETSLVSQKW
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEEEECCCCCCHHHHHHHHH
ARALGLVVIDQVAST
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]