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The map label for this gene is nth [H]

Identifier: 126208362

GI number: 126208362

Start: 1025502

End: 1026134

Strand: Direct

Name: nth [H]

Synonym: APL_0886

Alternate gene names: 126208362

Gene position: 1025502-1026134 (Clockwise)

Preceding gene: 126208361

Following gene: 126208363

Centisome position: 45.09

GC content: 44.55

Gene sequence:

>633_bases
ATGAATCAAGCAAAACGAATTGAAATTTTAACCCGTTTACGCAATGAAAATCCGCATCCGACCACGGAGCTGAATTACAG
TAATCCGTTTGAATTACTGATTGCGGTTATTCTTTCCGCCCAAGCAACCGATAAAGGGGTAAATAAAGCGACCGATAAAT
TGTTTCCGGTCGCTAACACGCCGCAAGCCATTTTAGATTTAGGCGTGGACGGTTTGAAGGAATATATTAAAACTATCGGG
CTTTTTAACAGTAAAGCGGAAAACATCATCAAAACCTGCCGTGATTTAATTGAAAAACATAACGGTGAAGTGCCGGAAAA
TCGCGAAGCCTTGGAAGCGCTTGCCGGTGTCGGGCGTAAAACCGCAAACGTCGTACTCAATACCGCCTTCGGTCACCCGA
CCATTGCGGTCGATACGCATATTTTTCGCGTGTCCAACCGTACCGGATTCGCTCCCGGTAAAGACGTAGTCAAAGTGGAA
GAAAAATTACTTAAAGTCGTCCCCGCCGAATTCAAAGTGGACGTACATCATTGGCTAATTTTACACGGTCGCTACACCTG
CATTGCCCGCAAGCCTCGTTGCGGTTCTTGTATTATTGAAGATTTATGCGAATACAAAGAGAAAAGCGAATAA

Upstream 100 bases:

>100_bases
AGGTAAAAAACTGGTACGTCCGATGTTAGTTACCATGTCATTTCTTATGGTAATCAAAATGTTATACGAACAAGGATTCT
TATCTTTTCTAGGAATATAA

Downstream 100 bases:

>100_bases
CTATTCTCTGTCTTGCGTAACACGCGGTGATATTTTCCCGAGGTTTTGCAAAATATTGTGAAAATATCATCGCTTGTCGT
ATATCATTTCGCTCTGCATA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNQAKRIEILTRLRNENPHPTTELNYSNPFELLIAVILSAQATDKGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKSE

Sequences:

>Translated_210_residues
MNQAKRIEILTRLRNENPHPTTELNYSNPFELLIAVILSAQATDKGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKSE
>Mature_210_residues
MNQAKRIEILTRLRNENPHPTTELNYSNPFELLIAVILSAQATDKGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKSE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=30.7262569832402, Blast_Score=88, Evalue=5e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=85.0961538461538, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=31.5508021390374, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23422; Mature: 23422

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAKRIEILTRLRNENPHPTTELNYSNPFELLIAVILSAQATDKGVNKATDKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCC
PQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVEEKLLKVVPAEFKVDVHHWLI
HHHHEEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEEHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKSE
EECCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQAKRIEILTRLRNENPHPTTELNYSNPFELLIAVILSAQATDKGVNKATDKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCC
PQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVEEKLLKVVPAEFKVDVHHWLI
HHHHEEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEEHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKSE
EECCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]