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The map label for this gene is fadI [H]

Identifier: 126208363

GI number: 126208363

Start: 1026344

End: 1027648

Strand: Direct

Name: fadI [H]

Synonym: APL_0887

Alternate gene names: 126208363

Gene position: 1026344-1027648 (Clockwise)

Preceding gene: 126208362

Following gene: 126208364

Centisome position: 45.12

GC content: 46.13

Gene sequence:

>1305_bases
ATGCCGAATCAGCAGTTACTGACAACCAAAGGCGAGCGTGTTGCGATTGTATCGGGGCTAAGAACGCCGTTTGCGCGTAA
AGATACCGGCTTTAAAGATGCGTATGCCACCAGCCTTGGTACGATGGTAACCAACGAATTACTCAGCCGCACGGCGATTG
AACATCACGAAATCGAACAACTGGTCTTCGGGCAAGTAATTCAGCAACCCGACATTCCGAATCCGGCAAGAGAAATCGCA
TTGGCATTGAATATGCCTAAATTACAATCTTACAGTATCAGTAGTTCTTGTTTATCCGGTTTACAAGCGATTGCCAATGT
TGCCGGCAGTATTGTCAGCGGTTCGATTAGTGCAGGTATTGCCGGAGGAGCCGATTCAATTTCAAATGCGCCGTTAAGTA
TCAGTCCTCGGGTTATTTATAAATTAAAATCTATTTTTAATGCGGAAACGCTCGAGGAAAAATATCGCCGTTTTCGTCAT
TTTTCTTGGCGAGATCTCAAACCGCACGGCGTTAATTTACGTGATTTCACTACACAAATGTCAGTAGCGGAAGTATCCGA
ACAAATGGCGCAAGATAATCACATAAGCCGAGCGGAACAAGACGAATACGCTCGCTTATCCAATCAAAGAGCCTCCGACG
CTTGGAAAATCGGTTTATTAAAAGAAGAAGTGATGCCGTCTTTTCCTCGCCCTTATAAAGATTTTGTCGTTTCGGATACG
TTAATTTCCGCGGCAACTCGAGCAAGCTATTACCAACGTTTCTCGCCGATTGTCGATAAAGAATATGCCACCGTCACCGA
AGCCAATATGCCTCAACCGATGGACGGAGCCGCCGCCGTGCTGTTAATGAACGAGTCGAGAGCTAAAGCACTCGGTTTAA
TGCCGTTAGGCTATATTCGCAGTTATGCGATTACCGGTAACGATATTTGGCAAGATATGTTCGCCGGTGCAACCATCGCC
GCCGCGCAAGCGTTGGATCGCGCCGAATTAAGTTTGTCCGATATGGATTACATTGATATCCACGAATCGTCCGCCAGCCA
AATATTAGCGAATATTCGCTTTTTCGAATCGGACGACTTTGCAAAAAAACAGCTAAATCGAACCGCTTGTTTAGGTAAAA
TCGATATGGATAAACTTAATCATTTAGGCGGCTCTATCGCTTTCGGCAATCCTCGTGCGGTAACCAGTTTGCGTACCGTG
ATTCAGTCACTCTATGCGCTAAAACGTAAAGGCGGCGGTATTTCATTAGTCGCCTCCGGCGGTTTAGGCGGCTTAGGGGC
GGCAATGGTATTAGAAAGCGAATAA

Upstream 100 bases:

>100_bases
TTCGTGCGTAGTTCTTTTCTTATGCTTAATCTATTTTCAAACTAACAATGCTACAATGTTATTTTCCAATTCCGACTTTC
CTCTTGATTAAGGACAAATC

Downstream 100 bases:

>100_bases
GGACAATCCATGACAGAACAACCCATTCAGCAACCGATGTTTCAAGTCGAAGTAGATGAAAATCAGATTGCCATCATTCG
CATTCACTCTATTGATAATG

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPNQQLLTTKGERVAIVSGLRTPFARKDTGFKDAYATSLGTMVTNELLSRTAIEHHEIEQLVFGQVIQQPDIPNPAREIA
LALNMPKLQSYSISSSCLSGLQAIANVAGSIVSGSISAGIAGGADSISNAPLSISPRVIYKLKSIFNAETLEEKYRRFRH
FSWRDLKPHGVNLRDFTTQMSVAEVSEQMAQDNHISRAEQDEYARLSNQRASDAWKIGLLKEEVMPSFPRPYKDFVVSDT
LISAATRASYYQRFSPIVDKEYATVTEANMPQPMDGAAAVLLMNESRAKALGLMPLGYIRSYAITGNDIWQDMFAGATIA
AAQALDRAELSLSDMDYIDIHESSASQILANIRFFESDDFAKKQLNRTACLGKIDMDKLNHLGGSIAFGNPRAVTSLRTV
IQSLYALKRKGGGISLVASGGLGGLGAAMVLESE

Sequences:

>Translated_434_residues
MPNQQLLTTKGERVAIVSGLRTPFARKDTGFKDAYATSLGTMVTNELLSRTAIEHHEIEQLVFGQVIQQPDIPNPAREIA
LALNMPKLQSYSISSSCLSGLQAIANVAGSIVSGSISAGIAGGADSISNAPLSISPRVIYKLKSIFNAETLEEKYRRFRH
FSWRDLKPHGVNLRDFTTQMSVAEVSEQMAQDNHISRAEQDEYARLSNQRASDAWKIGLLKEEVMPSFPRPYKDFVVSDT
LISAATRASYYQRFSPIVDKEYATVTEANMPQPMDGAAAVLLMNESRAKALGLMPLGYIRSYAITGNDIWQDMFAGATIA
AAQALDRAELSLSDMDYIDIHESSASQILANIRFFESDDFAKKQLNRTACLGKIDMDKLNHLGGSIAFGNPRAVTSLRTV
IQSLYALKRKGGGISLVASGGLGGLGAAMVLESE
>Mature_433_residues
PNQQLLTTKGERVAIVSGLRTPFARKDTGFKDAYATSLGTMVTNELLSRTAIEHHEIEQLVFGQVIQQPDIPNPAREIAL
ALNMPKLQSYSISSSCLSGLQAIANVAGSIVSGSISAGIAGGADSISNAPLSISPRVIYKLKSIFNAETLEEKYRRFRHF
SWRDLKPHGVNLRDFTTQMSVAEVSEQMAQDNHISRAEQDEYARLSNQRASDAWKIGLLKEEVMPSFPRPYKDFVVSDTL
ISAATRASYYQRFSPIVDKEYATVTEANMPQPMDGAAAVLLMNESRAKALGLMPLGYIRSYAITGNDIWQDMFAGATIAA
AQALDRAELSLSDMDYIDIHESSASQILANIRFFESDDFAKKQLNRTACLGKIDMDKLNHLGGSIAFGNPRAVTSLRTVI
QSLYALKRKGGGISLVASGGLGGLGAAMVLESE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=32.3040380047506, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4501853, Length=437, Percent_Identity=31.350114416476, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI167614485, Length=425, Percent_Identity=29.1764705882353, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI4557237, Length=422, Percent_Identity=27.0142180094787, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI148539872, Length=427, Percent_Identity=27.4004683840749, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1788683, Length=430, Percent_Identity=47.906976744186, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=27.6346604215457, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788554, Length=431, Percent_Identity=29.4663573085847, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=29.5774647887324, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI48994986, Length=416, Percent_Identity=28.8461538461538, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=30.6603773584906, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=427, Percent_Identity=25.5269320843091, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25147385, Length=422, Percent_Identity=25.3554502369668, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535921, Length=413, Percent_Identity=24.9394673123487, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325229, Length=429, Percent_Identity=25.6410256410256, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322031, Length=437, Percent_Identity=27.4599542334096, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137578, Length=436, Percent_Identity=30.2752293577982, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=28.5377358490566, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24640423, Length=431, Percent_Identity=24.1299303944316, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17648125, Length=435, Percent_Identity=25.7471264367816, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 47280; Mature: 47149

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00098 THIOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQQLLTTKGERVAIVSGLRTPFARKDTGFKDAYATSLGTMVTNELLSRTAIEHHEIEQ
CCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFGQVIQQPDIPNPAREIALALNMPKLQSYSISSSCLSGLQAIANVAGSIVSGSISAGI
HHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC
AGGADSISNAPLSISPRVIYKLKSIFNAETLEEKYRRFRHFSWRDLKPHGVNLRDFTTQM
CCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
SVAEVSEQMAQDNHISRAEQDEYARLSNQRASDAWKIGLLKEEVMPSFPRPYKDFVVSDT
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHEECHHHHHHCCCCCCCHHHHHHHHH
LISAATRASYYQRFSPIVDKEYATVTEANMPQPMDGAAAVLLMNESRAKALGLMPLGYIR
HHHHHHHHHHHHHHCCHHCCCHHEEECCCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHH
SYAITGNDIWQDMFAGATIAAAQALDRAELSLSDMDYIDIHESSASQILANIRFFESDDF
HEEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHEECCCHH
AKKQLNRTACLGKIDMDKLNHLGGSIAFGNPRAVTSLRTVIQSLYALKRKGGGISLVASG
HHHHHHHHHHCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GLGGLGAAMVLESE
CCCCCCEEEEEECC
>Mature Secondary Structure 
PNQQLLTTKGERVAIVSGLRTPFARKDTGFKDAYATSLGTMVTNELLSRTAIEHHEIEQ
CCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFGQVIQQPDIPNPAREIALALNMPKLQSYSISSSCLSGLQAIANVAGSIVSGSISAGI
HHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC
AGGADSISNAPLSISPRVIYKLKSIFNAETLEEKYRRFRHFSWRDLKPHGVNLRDFTTQM
CCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
SVAEVSEQMAQDNHISRAEQDEYARLSNQRASDAWKIGLLKEEVMPSFPRPYKDFVVSDT
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHEECHHHHHHCCCCCCCHHHHHHHHH
LISAATRASYYQRFSPIVDKEYATVTEANMPQPMDGAAAVLLMNESRAKALGLMPLGYIR
HHHHHHHHHHHHHHCCHHCCCHHEEECCCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHH
SYAITGNDIWQDMFAGATIAAAQALDRAELSLSDMDYIDIHESSASQILANIRFFESDDF
HEEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHEECCCHH
AKKQLNRTACLGKIDMDKLNHLGGSIAFGNPRAVTSLRTVIQSLYALKRKGGGISLVASG
HHHHHHHHHHCCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GLGGLGAAMVLESE
CCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA