Click here to switch to the map view.

The map label for this gene is pepE

Identifier: 126208347

GI number: 126208347

Start: 1011189

End: 1011893

Strand: Reverse

Name: pepE

Synonym: APL_0871

Alternate gene names: 126208347

Gene position: 1011893-1011189 (Counterclockwise)

Preceding gene: 126208351

Following gene: 126208346

Centisome position: 44.49

GC content: 40.57

Gene sequence:

>705_bases
ATGAAAAATATGTTGTTAATGAGCGGATCCAAATATAAGGATACGGCTTATCTAGTACATACATTACCGTGGTTAGCGCA
ATTCTTAGCAGATTATAAAGGTAAGAAAGTCGCTTTTGTACCTTATGCGGGAGTACGTCGTTCTTTCGATGAATATGAAA
CGACCGTAAAAAACGCATTACAATCTTTAGAGCTTGAGATCGTATCGGTGCATCGAGGTAAACAACATCGCGATATTATC
GAACAAGCGGATGTAATCGCAATTGGCGGCGGTAATACGTTTTGTTTATTGAAGCAAATGTATGAGCATGACTTACTGGA
TGCCATTCGAGCCAAAGTCAATAGCGGAACGCCTTATTTCGGCTGGAGTGCCGGTGCGAATGTCGCAGGCAGCTCGATTA
TGACTACCAATGATATGCCGATTACTTACCCGCCGTCTTTTAATGCGTTAAATCTCTTTCCGCATCAAATTAATCCGCAC
TTTATTTCCGGCAAAATGCAAGGGCATAACGGCGAAAGTCGAGAAGAGCGTTTAGAAGAATTTTTAATCGTTAATCCGCA
ATCTTTGGTTTATGCAATGCCGGAAGGTACCGCATTACATATTCAAGGCGAACAAGCGACCGTACTTGGTGCACAAGATA
TTCTCTGTTTTAGTGAAAAAATGCAACTTGATACTAAAGCGGTAAATTCAACATTTAATTACTAA

Upstream 100 bases:

>100_bases
GGCTATTTTGTGACAACTGTCAAAAAATAGAAGTTTTGTTTTTGCTTTTTATTTATCTAACGGGATAATGAGAACAAATC
GAATATAGAGGAGAGTCGAT

Downstream 100 bases:

>100_bases
GGAATTTATTATGGATGAACTCAAACCTTTTGTTGCCATTCTCGGCATTGTGGTCACGGTTTACCTGTTGATTAAAAAAT
ATGAAACCCGAACGGTATTA

Product: peptidase E

Products: NA

Alternate protein names: Alpha-aspartyl dipeptidase; Asp-specific dipeptidase; Dipeptidase E

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKNMLLMSGSKYKDTAYLVHTLPWLAQFLADYKGKKVAFVPYAGVRRSFDEYETTVKNALQSLELEIVSVHRGKQHRDII
EQADVIAIGGGNTFCLLKQMYEHDLLDAIRAKVNSGTPYFGWSAGANVAGSSIMTTNDMPITYPPSFNALNLFPHQINPH
FISGKMQGHNGESREERLEEFLIVNPQSLVYAMPEGTALHIQGEQATVLGAQDILCFSEKMQLDTKAVNSTFNY

Sequences:

>Translated_234_residues
MKNMLLMSGSKYKDTAYLVHTLPWLAQFLADYKGKKVAFVPYAGVRRSFDEYETTVKNALQSLELEIVSVHRGKQHRDII
EQADVIAIGGGNTFCLLKQMYEHDLLDAIRAKVNSGTPYFGWSAGANVAGSSIMTTNDMPITYPPSFNALNLFPHQINPH
FISGKMQGHNGESREERLEEFLIVNPQSLVYAMPEGTALHIQGEQATVLGAQDILCFSEKMQLDTKAVNSTFNY
>Mature_234_residues
MKNMLLMSGSKYKDTAYLVHTLPWLAQFLADYKGKKVAFVPYAGVRRSFDEYETTVKNALQSLELEIVSVHRGKQHRDII
EQADVIAIGGGNTFCLLKQMYEHDLLDAIRAKVNSGTPYFGWSAGANVAGSSIMTTNDMPITYPPSFNALNLFPHQINPH
FISGKMQGHNGESREERLEEFLIVNPQSLVYAMPEGTALHIQGEQATVLGAQDILCFSEKMQLDTKAVNSTFNY

Specific function: Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids

COG id: COG3340

COG function: function code E; Peptidase E

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family

Homologues:

Organism=Escherichia coli, GI1790452, Length=215, Percent_Identity=42.3255813953488, Blast_Score=177, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24641669, Length=204, Percent_Identity=44.1176470588235, Blast_Score=161, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPE_ACTP2 (A3N0N1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053572.1
- ProteinModelPortal:   A3N0N1
- SMR:   A3N0N1
- STRING:   A3N0N1
- MEROPS:   S51.001
- GeneID:   4850173
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0871
- eggNOG:   COG3340
- HOGENOM:   HBG298965
- OMA:   ALWRQGD
- PhylomeDB:   A3N0N1
- ProtClustDB:   PRK05282
- BioCyc:   APLE416269:APL_0871-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00510
- InterPro:   IPR005320
- InterPro:   IPR023172

Pfam domain/function: PF03575 Peptidase_S51

EC number: =3.4.13.21

Molecular weight: Translated: 26125; Mature: 26125

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 123-123 ACT_SITE 138-138 ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMLLMSGSKYKDTAYLVHTLPWLAQFLADYKGKKVAFVPYAGVRRSFDEYETTVKNAL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHH
QSLELEIVSVHRGKQHRDIIEQADVIAIGGGNTFCLLKQMYEHDLLDAIRAKVNSGTPYF
HHHHEEEEEECCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GWSAGANVAGSSIMTTNDMPITYPPSFNALNLFPHQINPHFISGKMQGHNGESREERLEE
CCCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCCEEEECEECCCCCCHHHHHHHH
FLIVNPQSLVYAMPEGTALHIQGEQATVLGAQDILCFSEKMQLDTKAVNSTFNY
HEEECCHHEEEECCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNMLLMSGSKYKDTAYLVHTLPWLAQFLADYKGKKVAFVPYAGVRRSFDEYETTVKNAL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHH
QSLELEIVSVHRGKQHRDIIEQADVIAIGGGNTFCLLKQMYEHDLLDAIRAKVNSGTPYF
HHHHEEEEEECCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GWSAGANVAGSSIMTTNDMPITYPPSFNALNLFPHQINPHFISGKMQGHNGESREERLEE
CCCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCCEEEECEECCCCCCHHHHHHHH
FLIVNPQSLVYAMPEGTALHIQGEQATVLGAQDILCFSEKMQLDTKAVNSTFNY
HEEECCHHEEEECCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA