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The map label for this gene is mnmC

Identifier: 126208334

GI number: 126208334

Start: 997904

End: 999919

Strand: Reverse

Name: mnmC

Synonym: APL_0858

Alternate gene names: 126208334

Gene position: 999919-997904 (Counterclockwise)

Preceding gene: 126208335

Following gene: 126208331

Centisome position: 43.96

GC content: 41.91

Gene sequence:

>2016_bases
GTGGTAAATGTTATGAACACACTCAGTTTTGCATCTCTTTCTTTCAATTCAAATAATACCCCCGTTTCCGAACAATTCGA
CGATATTTACTTTTCCACCCAAGACGGTCTTGAAGAAAGCTATTATGTTTTCCAAGATGGCAATCAACTTTGGCAAAAAT
GGCAAACACACGATGTTGAATCTTTTGTGATTGCCGAAACCGGCTTTGGCACCGGACTGAATTTTTTGGCGGTTGCCGAT
AAGTTTCAGCAGTTTTTAAGTGAGTTCCCCAATAGCAAACTTAAGCGTTTATATTTTATTTCGTTTGAAAAATTTCCCCT
CACTTCTGAGCAATTAGCGACCATTCATAAAAATTACCCGCAATTTGCAACACTTTCACAAAAAATGACCGCTTGTTGGC
AACCTCGCCAAACAGGCTGTCAGCGTTATCATTTCGAACAAATTTATCTTGATGTATGGTTTGGCGATATGTTAGACAAT
TTACCGCAATTAGGTGATTTATATACGAATCGAATTGATGCGTGGTTTTTAGACGGCTTTTCACCGGATAAAAATCCGGA
AATGTGGAATGAAACGTTATATCGCCAAATGTTTAGTCTGACTAAAAATGGTGGCAGTTTTGCAACGTTTACTGCCGCAA
GTACAGTGAGAAAAGGCTTACAAGCGGTCGGATTTGAGGTCAAAAAACGCAAAGGTTTTGGCAAAAAACGAGAAATGCTG
TGGGGTGAAAAACCGCAACAAAGCGAAACGGCACCGGTAAATTATCCGTATTTTTATAGTGAATCGCAAACCGAGGCGAA
TGATATTGCGGTTGTCGGAGGCGGTGTCGCCAGCTTGTTTGTTGTACTATCTTTACTGGAAAAAGGAAAAAAAGTCACGC
TTTATTGTAAAGATAATGCGTTGGCACAAAATGCTTCAGGCAATTTGCAAGGTGCGATCTATCCTCAACTGAGTGACGAT
GACGAGCGCAACATCCGTTTTTATGTGCATTGCTTTGATTATGCGTTGCAACGTTTGGCACAAATTGAACCGCTTGTTAA
TTTCGAGCATGCTTTAACCGGTGTAGCGTTATATGCCTATAACGATAAAACGGCGAAGAAATTGGAAAAAATAGCTCGGC
AAACCAATGATGATAGTTTGTTTAAGCTATGTAGTGCGGCTGAGTTAAGCGAAAAAATCGGGCTGAAAGTGCCGAACGGC
GGGGCATTTATGCCACAAAGCGGTTGGTTATCACCGATACAATTTGTACAGGGTACGTTTGCTTATTTACAAACGAAAGG
GTTACGGATTGTGCTAAATCACGAAGTGAAAGATCCGCAATTTAGTGAAGGGAAGTGGCATTGGCAGCACAATGGTAAAA
CGTTCAGTCATCAGATTTTAGTGTTGGCAAACGGACATACGCTCACGCAATTTCAGCAAGCTCAGGGGATTCCGCTTTAT
CCGGTGCGTGGGCAAGTGAGCCAGATTCCGACTACATCGGCTTTACAGCAGCTAAAATGTGTGGTGTGTTATGACGGTTA
TTTAACCCCAGTTTCTAAAGCCAATACCCATTGTATCGGTGCGAGCCATGTGCGTGATAATGCCGAAACGCATTTTTCGC
TGGAAGAGCACCATGAGAATGTCGCAAAACTTCAGCAAAATTTGACCGCTTGTGATTGGACTCAAGGTATTGATGAGTCA
CAGAATCTAGCAAAACAAGGGGTGCGAGCCGCATTACGAGATCGAGTGCCGATGGTTGGACAAATGCCGAATTTTAGCGT
TCAAAAAGTACAATATCAGAATTTGTATAATCAGCTACGCCGTAAACAAGCGGTCGAAAATGCGGCAAATTTTGCAAATT
TATATATGGTAAACGGTTTAGCTTCTCGAGGCTTAACCACCGCACCGCTATTAGGCGAAATGCTAGCGAGTTTGATCTGT
GATGAACCGTTACCAATCAGTGAAGATATTTGGCACGTACTCAGTCCAAATCGTACTTGGATACGAAAATTGTTGAAGGG
ATCAAAAGTAGAATAG

Upstream 100 bases:

>100_bases
TCGTAATTTTCGCTTACAATATCTTCTAATCTGTTACTGCGGAAAAGCACGGAAAGCACAGAGGGCGTTACAGTGACTTC
CGTGCTTTTCCGTTTATTCC

Downstream 100 bases:

>100_bases
TTAAAAAAACAAAACCTCGAATAATGATATTCGAGGTTTTTTAATTCATTATCCTAGTTATTTTCTTGTTAGTCCTTAGC
TCCCCAGCTTACTAATAAGA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MVNVMNTLSFASLSFNSNNTPVSEQFDDIYFSTQDGLEESYYVFQDGNQLWQKWQTHDVESFVIAETGFGTGLNFLAVAD
KFQQFLSEFPNSKLKRLYFISFEKFPLTSEQLATIHKNYPQFATLSQKMTACWQPRQTGCQRYHFEQIYLDVWFGDMLDN
LPQLGDLYTNRIDAWFLDGFSPDKNPEMWNETLYRQMFSLTKNGGSFATFTAASTVRKGLQAVGFEVKKRKGFGKKREML
WGEKPQQSETAPVNYPYFYSESQTEANDIAVVGGGVASLFVVLSLLEKGKKVTLYCKDNALAQNASGNLQGAIYPQLSDD
DERNIRFYVHCFDYALQRLAQIEPLVNFEHALTGVALYAYNDKTAKKLEKIARQTNDDSLFKLCSAAELSEKIGLKVPNG
GAFMPQSGWLSPIQFVQGTFAYLQTKGLRIVLNHEVKDPQFSEGKWHWQHNGKTFSHQILVLANGHTLTQFQQAQGIPLY
PVRGQVSQIPTTSALQQLKCVVCYDGYLTPVSKANTHCIGASHVRDNAETHFSLEEHHENVAKLQQNLTACDWTQGIDES
QNLAKQGVRAALRDRVPMVGQMPNFSVQKVQYQNLYNQLRRKQAVENAANFANLYMVNGLASRGLTTAPLLGEMLASLIC
DEPLPISEDIWHVLSPNRTWIRKLLKGSKVE

Sequences:

>Translated_671_residues
MVNVMNTLSFASLSFNSNNTPVSEQFDDIYFSTQDGLEESYYVFQDGNQLWQKWQTHDVESFVIAETGFGTGLNFLAVAD
KFQQFLSEFPNSKLKRLYFISFEKFPLTSEQLATIHKNYPQFATLSQKMTACWQPRQTGCQRYHFEQIYLDVWFGDMLDN
LPQLGDLYTNRIDAWFLDGFSPDKNPEMWNETLYRQMFSLTKNGGSFATFTAASTVRKGLQAVGFEVKKRKGFGKKREML
WGEKPQQSETAPVNYPYFYSESQTEANDIAVVGGGVASLFVVLSLLEKGKKVTLYCKDNALAQNASGNLQGAIYPQLSDD
DERNIRFYVHCFDYALQRLAQIEPLVNFEHALTGVALYAYNDKTAKKLEKIARQTNDDSLFKLCSAAELSEKIGLKVPNG
GAFMPQSGWLSPIQFVQGTFAYLQTKGLRIVLNHEVKDPQFSEGKWHWQHNGKTFSHQILVLANGHTLTQFQQAQGIPLY
PVRGQVSQIPTTSALQQLKCVVCYDGYLTPVSKANTHCIGASHVRDNAETHFSLEEHHENVAKLQQNLTACDWTQGIDES
QNLAKQGVRAALRDRVPMVGQMPNFSVQKVQYQNLYNQLRRKQAVENAANFANLYMVNGLASRGLTTAPLLGEMLASLIC
DEPLPISEDIWHVLSPNRTWIRKLLKGSKVE
>Mature_671_residues
MVNVMNTLSFASLSFNSNNTPVSEQFDDIYFSTQDGLEESYYVFQDGNQLWQKWQTHDVESFVIAETGFGTGLNFLAVAD
KFQQFLSEFPNSKLKRLYFISFEKFPLTSEQLATIHKNYPQFATLSQKMTACWQPRQTGCQRYHFEQIYLDVWFGDMLDN
LPQLGDLYTNRIDAWFLDGFSPDKNPEMWNETLYRQMFSLTKNGGSFATFTAASTVRKGLQAVGFEVKKRKGFGKKREML
WGEKPQQSETAPVNYPYFYSESQTEANDIAVVGGGVASLFVVLSLLEKGKKVTLYCKDNALAQNASGNLQGAIYPQLSDD
DERNIRFYVHCFDYALQRLAQIEPLVNFEHALTGVALYAYNDKTAKKLEKIARQTNDDSLFKLCSAAELSEKIGLKVPNG
GAFMPQSGWLSPIQFVQGTFAYLQTKGLRIVLNHEVKDPQFSEGKWHWQHNGKTFSHQILVLANGHTLTQFQQAQGIPLY
PVRGQVSQIPTTSALQQLKCVVCYDGYLTPVSKANTHCIGASHVRDNAETHFSLEEHHENVAKLQQNLTACDWTQGIDES
QNLAKQGVRAALRDRVPMVGQMPNFSVQKVQYQNLYNQLRRKQAVENAANFANLYMVNGLASRGLTTAPLLGEMLASLIC
DEPLPISEDIWHVLSPNRTWIRKLLKGSKVE

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=665, Percent_Identity=41.6541353383459, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_ACTP2 (A3N0L8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053559.1
- ProteinModelPortal:   A3N0L8
- SMR:   A3N0L8
- STRING:   A3N0L8
- GeneID:   4849445
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0858
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A3N0L8
- ProtClustDB:   PRK01747
- BioCyc:   APLE416269:APL_0858-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 75953; Mature: 75953

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVMNTLSFASLSFNSNNTPVSEQFDDIYFSTQDGLEESYYVFQDGNQLWQKWQTHDVE
CCCHHHHHEEEEEEECCCCCCCHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHCCCCC
SFVIAETGFGTGLNFLAVADKFQQFLSEFPNSKLKRLYFISFEKFPLTSEQLATIHKNYP
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEEEEECCCCCHHHHHHHHHCCC
QFATLSQKMTACWQPRQTGCQRYHFEQIYLDVWFGDMLDNLPQLGDLYTNRIDAWFLDGF
CHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEHHHHHHHHCCHHHHHHHHHCCCEEEECCC
SPDKNPEMWNETLYRQMFSLTKNGGSFATFTAASTVRKGLQAVGFEVKKRKGFGKKREML
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
WGEKPQQSETAPVNYPYFYSESQTEANDIAVVGGGVASLFVVLSLLEKGKKVTLYCKDNA
CCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
LAQNASGNLQGAIYPQLSDDDERNIRFYVHCFDYALQRLAQIEPLVNFEHALTGVALYAY
CCCCCCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHHEEEEEEE
NDKTAKKLEKIARQTNDDSLFKLCSAAELSEKIGLKVPNGGAFMPQSGWLSPIQFVQGTF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AYLQTKGLRIVLNHEVKDPQFSEGKWHWQHNGKTFSHQILVLANGHTLTQFQQAQGIPLY
HHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHCCCCEEE
PVRGQVSQIPTTSALQQLKCVVCYDGYLTPVSKANTHCIGASHVRDNAETHFSLEEHHEN
CCCCCHHCCCCHHHHHHCEEEEEECCCCCCCCCCCCEEEEHHHHCCCCHHCCCHHHHHHH
VAKLQQNLTACDWTQGIDESQNLAKQGVRAALRDRVPMVGQMPNFSVQKVQYQNLYNQLR
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RKQAVENAANFANLYMVNGLASRGLTTAPLLGEMLASLICDEPLPISEDIWHVLSPNRTW
HHHHHHHHHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
IRKLLKGSKVE
HHHHHCCCCCC
>Mature Secondary Structure
MVNVMNTLSFASLSFNSNNTPVSEQFDDIYFSTQDGLEESYYVFQDGNQLWQKWQTHDVE
CCCHHHHHEEEEEEECCCCCCCHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHCCCCC
SFVIAETGFGTGLNFLAVADKFQQFLSEFPNSKLKRLYFISFEKFPLTSEQLATIHKNYP
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHCEEEEEEEECCCCCHHHHHHHHHCCC
QFATLSQKMTACWQPRQTGCQRYHFEQIYLDVWFGDMLDNLPQLGDLYTNRIDAWFLDGF
CHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEHHHHHHHHCCHHHHHHHHHCCCEEEECCC
SPDKNPEMWNETLYRQMFSLTKNGGSFATFTAASTVRKGLQAVGFEVKKRKGFGKKREML
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
WGEKPQQSETAPVNYPYFYSESQTEANDIAVVGGGVASLFVVLSLLEKGKKVTLYCKDNA
CCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
LAQNASGNLQGAIYPQLSDDDERNIRFYVHCFDYALQRLAQIEPLVNFEHALTGVALYAY
CCCCCCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHHEEEEEEE
NDKTAKKLEKIARQTNDDSLFKLCSAAELSEKIGLKVPNGGAFMPQSGWLSPIQFVQGTF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AYLQTKGLRIVLNHEVKDPQFSEGKWHWQHNGKTFSHQILVLANGHTLTQFQQAQGIPLY
HHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHCCCCEEE
PVRGQVSQIPTTSALQQLKCVVCYDGYLTPVSKANTHCIGASHVRDNAETHFSLEEHHEN
CCCCCHHCCCCHHHHHHCEEEEEECCCCCCCCCCCCEEEEHHHHCCCCHHCCCHHHHHHH
VAKLQQNLTACDWTQGIDESQNLAKQGVRAALRDRVPMVGQMPNFSVQKVQYQNLYNQLR
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RKQAVENAANFANLYMVNGLASRGLTTAPLLGEMLASLICDEPLPISEDIWHVLSPNRTW
HHHHHHHHHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
IRKLLKGSKVE
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA