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The map label for this gene is metF [H]

Identifier: 126208330

GI number: 126208330

Start: 991911

End: 992792

Strand: Direct

Name: metF [H]

Synonym: APL_0854

Alternate gene names: 126208330

Gene position: 991911-992792 (Clockwise)

Preceding gene: 126208328

Following gene: 126208332

Centisome position: 43.61

GC content: 39.68

Gene sequence:

>882_bases
ATGAGCTATGCGAAAGAAATCGATCACTTAAACCATACCCTTGCCGATTTAAACGGCAATATTAACGCTTCTTTTGAATT
TTTCCCCGCTAAAAACGAAAAAATGGAAATGTTACTTTGGGATTCGATTCATCGACTTGCTCCGCTTAAACCTAAATTCG
TTTCAGTTACCTATGGTGCAAATTCCGGCGAGCGAAAACGTACTCATTCAATTGTGAAACGTATTCAGCAAGATACCGGT
TTAGCTGCAGCACCGCATTTAACCGGTATTGATGCCAATCCCGAACAGCTCCGTATAATTGCGCAAGATTACTGGGAAAA
CGGTATTCGCCATATTGTTGCACTACGAGGTGACGAACCTGCCGGTTATGCTAAAAAATCCTTTTTTGCAAAAGATTTAG
TAAAACTCTTAAAAGAAGTCGCGGATTTTGATATTTCGGTTGCCGCCTATCCCGAAGTACATCCGGAGGCAAAATCGGCA
CAATCCGACTTAATTTACTTAAAACAAAAAGTGGAAGCCGGTGCTAATCGTGCGATTACCCAATTCTTTTTTGATATCGA
CAGTTATCTTCGATTCCGTGATCGCTGTGTAACCGTTGGAATTGATGTGGAAATCGTACCTGGCATTTTACCGGTCACGA
ATTTTAAACAATTACAAAAAATGACAAAAATTACCAATGTTAAAGTACCGCATTGGATGCATAAAATGTATGAAGGATTA
GATGATGATCAAACAACTAGAAATTTAGTTGCCGCCAGTATTGCCATGGATATGGTTAAAATTCTCTCAAAAGAGGGCGT
AAAAGATTTCCATTTCTACACACTGAATCACTCCGAATTAACCTATGCGATTTGCCATGCATTAGGCGTACGACCGACTT
AA

Upstream 100 bases:

>100_bases
ATAAAAATAATAAAAAACATGAAAATAATGAAATTGATTTAACTAAATCGGCATTTTAAAGTGTTCTTATTACATATCAG
AATAATATGGAGACACTTCT

Downstream 100 bases:

>100_bases
ATAGACAAAACGCACTAAATCCTAAAATTTAGTGCGTTTTTAAGTAACAACAAGCGGTTAAATTTTTATTTAAATTTACC
GAATTACCACTCGTTTTTAT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSYAKEIDHLNHTLADLNGNINASFEFFPAKNEKMEMLLWDSIHRLAPLKPKFVSVTYGANSGERKRTHSIVKRIQQDTG
LAAAPHLTGIDANPEQLRIIAQDYWENGIRHIVALRGDEPAGYAKKSFFAKDLVKLLKEVADFDISVAAYPEVHPEAKSA
QSDLIYLKQKVEAGANRAITQFFFDIDSYLRFRDRCVTVGIDVEIVPGILPVTNFKQLQKMTKITNVKVPHWMHKMYEGL
DDDQTTRNLVAASIAMDMVKILSKEGVKDFHFYTLNHSELTYAICHALGVRPT

Sequences:

>Translated_293_residues
MSYAKEIDHLNHTLADLNGNINASFEFFPAKNEKMEMLLWDSIHRLAPLKPKFVSVTYGANSGERKRTHSIVKRIQQDTG
LAAAPHLTGIDANPEQLRIIAQDYWENGIRHIVALRGDEPAGYAKKSFFAKDLVKLLKEVADFDISVAAYPEVHPEAKSA
QSDLIYLKQKVEAGANRAITQFFFDIDSYLRFRDRCVTVGIDVEIVPGILPVTNFKQLQKMTKITNVKVPHWMHKMYEGL
DDDQTTRNLVAASIAMDMVKILSKEGVKDFHFYTLNHSELTYAICHALGVRPT
>Mature_292_residues
SYAKEIDHLNHTLADLNGNINASFEFFPAKNEKMEMLLWDSIHRLAPLKPKFVSVTYGANSGERKRTHSIVKRIQQDTGL
AAAPHLTGIDANPEQLRIIAQDYWENGIRHIVALRGDEPAGYAKKSFFAKDLVKLLKEVADFDISVAAYPEVHPEAKSAQ
SDLIYLKQKVEAGANRAITQFFFDIDSYLRFRDRCVTVGIDVEIVPGILPVTNFKQLQKMTKITNVKVPHWMHKMYEGLD
DDQTTRNLVAASIAMDMVKILSKEGVKDFHFYTLNHSELTYAICHALGVRPT

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=289, Percent_Identity=33.9100346020761, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1790377, Length=290, Percent_Identity=71.7241379310345, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=29.6428571428571, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=29.6428571428571, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.2925170068027, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321313, Length=293, Percent_Identity=29.6928327645051, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33159; Mature: 33028

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAKEIDHLNHTLADLNGNINASFEFFPAKNEKMEMLLWDSIHRLAPLKPKFVSVTYGA
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
NSGERKRTHSIVKRIQQDTGLAAAPHLTGIDANPEQLRIIAQDYWENGIRHIVALRGDEP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCC
AGYAKKSFFAKDLVKLLKEVADFDISVAAYPEVHPEAKSAQSDLIYLKQKVEAGANRAIT
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
QFFFDIDSYLRFRDRCVTVGIDVEIVPGILPVTNFKQLQKMTKITNVKVPHWMHKMYEGL
HHHHHHHHHHHHHHCEEEEECCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC
DDDQTTRNLVAASIAMDMVKILSKEGVKDFHFYTLNHSELTYAICHALGVRPT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SYAKEIDHLNHTLADLNGNINASFEFFPAKNEKMEMLLWDSIHRLAPLKPKFVSVTYGA
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
NSGERKRTHSIVKRIQQDTGLAAAPHLTGIDANPEQLRIIAQDYWENGIRHIVALRGDEP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCC
AGYAKKSFFAKDLVKLLKEVADFDISVAAYPEVHPEAKSAQSDLIYLKQKVEAGANRAIT
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
QFFFDIDSYLRFRDRCVTVGIDVEIVPGILPVTNFKQLQKMTKITNVKVPHWMHKMYEGL
HHHHHHHHHHHHHHCEEEEECCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC
DDDQTTRNLVAASIAMDMVKILSKEGVKDFHFYTLNHSELTYAICHALGVRPT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]