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The map label for this gene is nadB [C]

Identifier: 126208328

GI number: 126208328

Start: 988874

End: 990472

Strand: Direct

Name: nadB [C]

Synonym: APL_0852

Alternate gene names: 126208328

Gene position: 988874-990472 (Clockwise)

Preceding gene: 126208327

Following gene: 126208330

Centisome position: 43.48

GC content: 42.84

Gene sequence:

>1599_bases
ATGCACTCGATTCAAATTACTCAAACGATTTATACTGATTTACTGATTGTCGGTTCAGGTATTGCTGGGCTCGCGGCGGC
AATTGAAGCGGAACGACTTGGGATGAAAACGACGTTAATCAGCAAAACGCCTATTGGTTCGGGAGCAAGTTTCTTTCCGT
TAAAAGCCACACTGGGCATTCAAGTGACCGGCAAAAATGATGCGGAAAAATTCCGGCAAGATATTGAGCGTGTCGGCAAA
GGCAGAACCAATCCGAAAATAGTCAAAGCCTATATTGAAGATTCGCCTCAAGCAATTGGATTACTCGCAAAAATCGGCTT
TAAACCTTGGCTACGCAACGATAATCGCCCGGCCTGTTTCGCCAAGTATCCCCGCCCTATTTATCTGATCAATAATTGGC
GGGAAACCGCTCAACGGGCGAAACAAATTCTTGATAGCCAATCTACCGACGTTTATGAACAAGCGACACTACTGCATATT
GTTACCGAACAAAATCAAGTACAAGGAGCGGTGTTTAGCCTGAATACAAGCGGTCAAATTTGCTATATTTTTTGCAAAAC
CAGCCAAATCATTTTAGCCAGCGGTGGCATTGCCGGCTTGTATAAAGACAATCTTTATCCGGCGGATATTATCGGCTCAA
CTCATTCTATCGCTCAACAAGCCGGTGCAAAATTGACTAATCTCGCCTTTATTCAATTTATCCCCTCTTTTGTCGAACCG
AAATACAAAGTGTTATTTGGCGAACATACGCTGAAATACGTCACTAAAATTACCGATGAAAACGGGCAAGATCTGTTCTC
GCATCTCACTCAAATACAATTTCAACAAATGATGTTAGCCCGCAGCCATTATGCGCCGTTTAGTGTGGATTTCCCTTGTG
TCGAATTCGATTTGGTGATGATGAAACATTTATTAGCTAACCCACAGCAAAAAGGCATTTACCTGCACTATAGCCCTGAG
CTTTATCAAGATGAACAAGAGTTTTATACGGTTTATCTTAATTGGTTACGCCAAGAAGTAGGGATAGATTTGGTTAAAGA
TAAAATTGCTATCGCACCGTTTGCACATAGCTGTAACGGCGGAATTGAGATTGATGAGTATGGCGAATCCTGTGTCGCCG
GATTATTTGCGATTGGCGAAATAGCCCATTGTATTGAGGGAGCAAATCGCTTAGGCGGTAATTCGGTCGGAGGGGGATTA
GTATTCGCCAAACGAGCGGTGGCACAAGCGGTCAAAAATTTGCAAAAATTTGCTGAAATCCAACCGCTTGCAAACACTGA
AACCTATCGTTTACAGGCTGAAAAAGTGCTTAATCAGTTAAACAATCCAATGGGAGATAATACGCTGCTTGCGAGTACGG
TACTCAAACAAATTCGTGAACAAATGGCTCGTTTTGCTAATGTGTATCGCACCAAAGCCAACTTAACCTTACTGTTGAAC
GAACTGCAACAGCTCGAAAAACGTTTTAATCCGCTTGCTCATCATCAGCAACAAGGCATCGAAATCTTTTATGCACTAAA
AACCGCACAGCTTGTCGTTTCTCAGATGCTAAACGAACCGCACAGTTTAGGTGCTCATTACTTAGCCGATAAGCAGTAA

Upstream 100 bases:

>100_bases
AGAGGAATACACTCGTACGCTGATTAATGCCGCACCGTCATTATTATAATTTATCTCATATTCAGGGCAGGTTTATCCTG
CCCTTTTACTTATACTGATT

Downstream 100 bases:

>100_bases
AACAAAATACAAAAATAAAGGGCAAGATTTTATCTTGCCCTTTTGATCATCCGTTTAGCGATTATTTACCCCAAACTTCT
TCGCTAATTTCACGAATAAA

Product: putative FAD flavoprotein oxidase

Products: oxaloacetate; NH3; H2O2; iminoaspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MHSIQITQTIYTDLLIVGSGIAGLAAAIEAERLGMKTTLISKTPIGSGASFFPLKATLGIQVTGKNDAEKFRQDIERVGK
GRTNPKIVKAYIEDSPQAIGLLAKIGFKPWLRNDNRPACFAKYPRPIYLINNWRETAQRAKQILDSQSTDVYEQATLLHI
VTEQNQVQGAVFSLNTSGQICYIFCKTSQIILASGGIAGLYKDNLYPADIIGSTHSIAQQAGAKLTNLAFIQFIPSFVEP
KYKVLFGEHTLKYVTKITDENGQDLFSHLTQIQFQQMMLARSHYAPFSVDFPCVEFDLVMMKHLLANPQQKGIYLHYSPE
LYQDEQEFYTVYLNWLRQEVGIDLVKDKIAIAPFAHSCNGGIEIDEYGESCVAGLFAIGEIAHCIEGANRLGGNSVGGGL
VFAKRAVAQAVKNLQKFAEIQPLANTETYRLQAEKVLNQLNNPMGDNTLLASTVLKQIREQMARFANVYRTKANLTLLLN
ELQQLEKRFNPLAHHQQQGIEIFYALKTAQLVVSQMLNEPHSLGAHYLADKQ

Sequences:

>Translated_532_residues
MHSIQITQTIYTDLLIVGSGIAGLAAAIEAERLGMKTTLISKTPIGSGASFFPLKATLGIQVTGKNDAEKFRQDIERVGK
GRTNPKIVKAYIEDSPQAIGLLAKIGFKPWLRNDNRPACFAKYPRPIYLINNWRETAQRAKQILDSQSTDVYEQATLLHI
VTEQNQVQGAVFSLNTSGQICYIFCKTSQIILASGGIAGLYKDNLYPADIIGSTHSIAQQAGAKLTNLAFIQFIPSFVEP
KYKVLFGEHTLKYVTKITDENGQDLFSHLTQIQFQQMMLARSHYAPFSVDFPCVEFDLVMMKHLLANPQQKGIYLHYSPE
LYQDEQEFYTVYLNWLRQEVGIDLVKDKIAIAPFAHSCNGGIEIDEYGESCVAGLFAIGEIAHCIEGANRLGGNSVGGGL
VFAKRAVAQAVKNLQKFAEIQPLANTETYRLQAEKVLNQLNNPMGDNTLLASTVLKQIREQMARFANVYRTKANLTLLLN
ELQQLEKRFNPLAHHQQQGIEIFYALKTAQLVVSQMLNEPHSLGAHYLADKQ
>Mature_532_residues
MHSIQITQTIYTDLLIVGSGIAGLAAAIEAERLGMKTTLISKTPIGSGASFFPLKATLGIQVTGKNDAEKFRQDIERVGK
GRTNPKIVKAYIEDSPQAIGLLAKIGFKPWLRNDNRPACFAKYPRPIYLINNWRETAQRAKQILDSQSTDVYEQATLLHI
VTEQNQVQGAVFSLNTSGQICYIFCKTSQIILASGGIAGLYKDNLYPADIIGSTHSIAQQAGAKLTNLAFIQFIPSFVEP
KYKVLFGEHTLKYVTKITDENGQDLFSHLTQIQFQQMMLARSHYAPFSVDFPCVEFDLVMMKHLLANPQQKGIYLHYSPE
LYQDEQEFYTVYLNWLRQEVGIDLVKDKIAIAPFAHSCNGGIEIDEYGESCVAGLFAIGEIAHCIEGANRLGGNSVGGGL
VFAKRAVAQAVKNLQKFAEIQPLANTETYRLQAEKVLNQLNNPMGDNTLLASTVLKQIREQMARFANVYRTKANLTLLLN
ELQQLEKRFNPLAHHQQQGIEIFYALKTAQLVVSQMLNEPHSLGAHYLADKQ

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788928, Length=352, Percent_Identity=23.8636363636364, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790597, Length=400, Percent_Identity=23.5, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322701, Length=413, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.4.3.16 [C]

Molecular weight: Translated: 59261; Mature: 59261

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSIQITQTIYTDLLIVGSGIAGLAAAIEAERLGMKTTLISKTPIGSGASFFPLKATLGI
CCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEEEE
QVTGKNDAEKFRQDIERVGKGRTNPKIVKAYIEDSPQAIGLLAKIGFKPWLRNDNRPACF
EEECCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AKYPRPIYLINNWRETAQRAKQILDSQSTDVYEQATLLHIVTEQNQVQGAVFSLNTSGQI
ECCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCEEEEECCCCCE
CYIFCKTSQIILASGGIAGLYKDNLYPADIIGSTHSIAQQAGAKLTNLAFIQFIPSFVEP
EEEEEECCEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC
KYKVLFGEHTLKYVTKITDENGQDLFSHLTQIQFQQMMLARSHYAPFSVDFPCVEFDLVM
CEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MKHLLANPQQKGIYLHYSPELYQDEQEFYTVYLNWLRQEVGIDLVKDKIAIAPFAHSCNG
HHHHHCCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCCC
GIEIDEYGESCVAGLFAIGEIAHCIEGANRLGGNSVGGGLVFAKRAVAQAVKNLQKFAEI
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QPLANTETYRLQAEKVLNQLNNPMGDNTLLASTVLKQIREQMARFANVYRTKANLTLLLN
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQQLEKRFNPLAHHQQQGIEIFYALKTAQLVVSQMLNEPHSLGAHYLADKQ
HHHHHHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHCCCHHHCCCC
>Mature Secondary Structure
MHSIQITQTIYTDLLIVGSGIAGLAAAIEAERLGMKTTLISKTPIGSGASFFPLKATLGI
CCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEEEE
QVTGKNDAEKFRQDIERVGKGRTNPKIVKAYIEDSPQAIGLLAKIGFKPWLRNDNRPACF
EEECCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AKYPRPIYLINNWRETAQRAKQILDSQSTDVYEQATLLHIVTEQNQVQGAVFSLNTSGQI
ECCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCEEEEECCCCCE
CYIFCKTSQIILASGGIAGLYKDNLYPADIIGSTHSIAQQAGAKLTNLAFIQFIPSFVEP
EEEEEECCEEEEECCCCCEEECCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC
KYKVLFGEHTLKYVTKITDENGQDLFSHLTQIQFQQMMLARSHYAPFSVDFPCVEFDLVM
CEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MKHLLANPQQKGIYLHYSPELYQDEQEFYTVYLNWLRQEVGIDLVKDKIAIAPFAHSCNG
HHHHHCCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCCC
GIEIDEYGESCVAGLFAIGEIAHCIEGANRLGGNSVGGGLVFAKRAVAQAVKNLQKFAEI
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QPLANTETYRLQAEKVLNQLNNPMGDNTLLASTVLKQIREQMARFANVYRTKANLTLLLN
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQQLEKRFNPLAHHQQQGIEIFYALKTAQLVVSQMLNEPHSLGAHYLADKQ
HHHHHHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: L-Aspartate [C]

Kcat value (1/min): 2 [C]

Specific activity: NA

Km value (mM): 4.1 {L-aspartate}} 0.5 {L-aspartate}} [C]

Substrates: @ASPA02txt*L-Aspartate!; H2O; O2; L-aspartate [C]

Specific reaction: @ASOA02.txt*L-Aspartate! + H2O + O2 = oxaloacetate + NH3 + H2O2 O2 + L-aspartate = H2O2 + iminoaspartate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: Iodoacetate; L-Aspartate; Mesotartrate; NAD+; Tetranitromethane; Urea [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]