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The map label for this gene is pntA [H]

Identifier: 126208318

GI number: 126208318

Start: 977528

End: 979066

Strand: Reverse

Name: pntA [H]

Synonym: APL_0842

Alternate gene names: 126208318

Gene position: 979066-977528 (Counterclockwise)

Preceding gene: 126208320

Following gene: 126208317

Centisome position: 43.05

GC content: 45.61

Gene sequence:

>1539_bases
ATGCTTATTGGTGTACCAAGAGAGCTGTTAGACGGTGAAACACGTGTGGCGGCAACGCCAAAAACCGTTGAACAGATCAA
AAAGCTCGGCTTTGATGTGCTCGTTGAACACAATGCGGGCTTTAAAGCGAGTTTTGAGGACAACGCATTTGTTAATGCAG
GTGCGACTGTCGGCACGCAACAAGAGGTTTGGAATTCAGATATTATTTTTAAAGTGAATGCACCGACCGATGCTGAAATC
GCCCTTATTAAAGAAGGCGCAACGCTAGTGAGTTTTATTTGGCCTGCACAAAATCCGCAATTAATGGAAAAATTGCAAGC
TAAAAAAATCAATGTATTGGCAATGGACGCTGTGCCTCGTATTTCGCGTGCACAAGCACTTGATGCATTGAGTTCAATGG
CGAATATTTCCGGCTATCGTGCGGTTATCGAAGCGGCGAATGCATTCGGTAGCTTCTTTACCGGTCAAATTACTGCAGCA
GGTAAAGTACCGCCGGCGAAAGTGCTGGTTATCGGTGCTGGTGTTGCCGGTTTAGCGGCAATCGGTGCGGCAAACAGCTT
AGGTGCGATTGTTCGTGCGTTTGACTCTCGTCCGGAAGTAAAAGAACAAGTGAAATCTATGGGTGCAGACTTCTTAGAAA
TCGATTTCGAAGAAGAAGGCGGCTCAGGCGACGGTTATGCAAAAGTAATGTCGGAAGAGTTTAACCGTCGTGCAATGGAA
CTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATCACTACGGCGGCGATTCCGGGTAAAGCGGCACCTCGTTTAAT
CACTAAAGAAATGGTCGATTCAATGAAACCGGGTTCAGTGGTTGTCGATTTAGCAGCAGCAACCGGCGGTAACTGTGAAT
ATACCAAAGCGGGTAAAGCGATTGTTACTAAAAATCAAGTAAAAGTTATCGGTTATACCGATTTCCCTGCACGTTTACCA
ACGCAATCTTCACAACTTTACGGTACAAACTTAGTTAATTTGCTTAAACTGCTTACACCGAATAAAGACGGTCAAATTGA
CATTAATTTCGAAGATGTGGTATTACGTGGCGTAACCGTAGTACGTGACGGCGAATTAACTTGGCCGGCTCCGCCGATTC
AAGTTTCGGCTCAACCGCAAAAACCAGCGGCTGCGGCACCGATTGAGAAAAAAGAAGAAAAACCGACCGATCCTCGTGTG
AAATACGGTGTGATGACAGGTGCCGGAGCGTTATTCTTATGGTTGGCATCCGTAGCGCCGGCGGCATTCTTATCACACTT
CACCGTATTCGTATTAGCCTGTGTGGTGGGATATTATGTGGTTTGGAACGTCAGCCACGCATTACATACCCCGTTAATGG
CGGTAACCAATGCGATTTCCGGCATTATCATTGTCGGTGCGGTGTTACAAATATCTCAGCCTACCGGTAATTTCTTCGTG
GATATTCTTGCGTTTATTGCGATTTTAGTCGCAAGCATTAATATCTTCGGCGGTTTCAAAGTCACGCAACGTATGCTTGC
GATGTTTAGAAAAGGTTAA

Upstream 100 bases:

>100_bases
ACAAATTTCTCCGATTTAATGATACATTTAGGCGAGTTTTTTAAAACGAAGTGGGTTATCATCATAGCCCGATTATAAAC
ATAACCATACAGGAGTCTTT

Downstream 100 bases:

>100_bases
GGAGAAACTCATGTCTTTTGGATTTGTAACGGCCGCATATATTATTGCTGCGATTCTCTTTATTATGAGTTTAGCAGGAC
TTTCTAAACACGAAACGGCA

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEHNAGFKASFEDNAFVNAGATVGTQQEVWNSDIIFKVNAPTDAEI
ALIKEGATLVSFIWPAQNPQLMEKLQAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKAAPRLITKEMVDSMKPGSVVVDLAAATGGNCEYTKAGKAIVTKNQVKVIGYTDFPARLP
TQSSQLYGTNLVNLLKLLTPNKDGQIDINFEDVVLRGVTVVRDGELTWPAPPIQVSAQPQKPAAAAPIEKKEEKPTDPRV
KYGVMTGAGALFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGAVLQISQPTGNFFV
DILAFIAILVASINIFGGFKVTQRMLAMFRKG

Sequences:

>Translated_512_residues
MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEHNAGFKASFEDNAFVNAGATVGTQQEVWNSDIIFKVNAPTDAEI
ALIKEGATLVSFIWPAQNPQLMEKLQAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKAAPRLITKEMVDSMKPGSVVVDLAAATGGNCEYTKAGKAIVTKNQVKVIGYTDFPARLP
TQSSQLYGTNLVNLLKLLTPNKDGQIDINFEDVVLRGVTVVRDGELTWPAPPIQVSAQPQKPAAAAPIEKKEEKPTDPRV
KYGVMTGAGALFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGAVLQISQPTGNFFV
DILAFIAILVASINIFGGFKVTQRMLAMFRKG
>Mature_512_residues
MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEHNAGFKASFEDNAFVNAGATVGTQQEVWNSDIIFKVNAPTDAEI
ALIKEGATLVSFIWPAQNPQLMEKLQAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKAAPRLITKEMVDSMKPGSVVVDLAAATGGNCEYTKAGKAIVTKNQVKVIGYTDFPARLP
TQSSQLYGTNLVNLLKLLTPNKDGQIDINFEDVVLRGVTVVRDGELTWPAPPIQVSAQPQKPAAAAPIEKKEEKPTDPRV
KYGVMTGAGALFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGAVLQISQPTGNFFV
DILAFIAILVASINIFGGFKVTQRMLAMFRKG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=537, Percent_Identity=48.7895716945996, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI122939153, Length=537, Percent_Identity=48.7895716945996, Blast_Score=462, Evalue=1e-130,
Organism=Escherichia coli, GI1787887, Length=512, Percent_Identity=73.4375, Blast_Score=736, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=51.1538461538461, Blast_Score=482, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR016040
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54537; Mature: 54537

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEHNAGFKASFEDNAFVNAGATVGTQ
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCCEEEEECCCCCEECCCCCCEEECCCCCCCH
QEVWNSDIIFKVNAPTDAEIALIKEGATLVSFIWPAQNPQLMEKLQAKKINVLAMDAVPR
HHHCCCCEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKAAPRLITKEMVDSMKPGSVVVDLAAATGGNCEYTKAGKA
HHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCE
IVTKNQVKVIGYTDFPARLPTQSSQLYGTNLVNLLKLLTPNKDGQIDINFEDVVLRGVTV
EEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHEECEEE
VRDGELTWPAPPIQVSAQPQKPAAAAPIEKKEEKPTDPRVKYGVMTGAGALFLWLASVAP
EECCCEECCCCCCEEECCCCCCCCCCCCCHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGAVLQISQPTGNFFV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
DILAFIAILVASINIFGGFKVTQRMLAMFRKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEHNAGFKASFEDNAFVNAGATVGTQ
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCCEEEEECCCCCEECCCCCCEEECCCCCCCH
QEVWNSDIIFKVNAPTDAEIALIKEGATLVSFIWPAQNPQLMEKLQAKKINVLAMDAVPR
HHHCCCCEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKAAPRLITKEMVDSMKPGSVVVDLAAATGGNCEYTKAGKA
HHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCE
IVTKNQVKVIGYTDFPARLPTQSSQLYGTNLVNLLKLLTPNKDGQIDINFEDVVLRGVTV
EEECCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHEECEEE
VRDGELTWPAPPIQVSAQPQKPAAAAPIEKKEEKPTDPRVKYGVMTGAGALFLWLASVAP
EECCCEECCCCCCEEECCCCCCCCCCCCCHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGAVLQISQPTGNFFV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
DILAFIAILVASINIFGGFKVTQRMLAMFRKG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]