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The map label for this gene is pckA

Identifier: 126208276

GI number: 126208276

Start: 922121

End: 923731

Strand: Reverse

Name: pckA

Synonym: APL_0800

Alternate gene names: 126208276

Gene position: 923731-922121 (Counterclockwise)

Preceding gene: 126208280

Following gene: 126208273

Centisome position: 40.61

GC content: 42.64

Gene sequence:

>1611_bases
ATGTTAAGTCGTATTGAACAAGAACTAGCACAATTAGGTATTACTAACGTAAAAGAAATTGTACGTAATCCGAGTTATGA
ACAATTATTCGAAGAAGAAATGAAACCTGAATTAGAAGGTTTTGAAAAAGGTCGTTTAACGACATCCGGTGCCGTTGCCG
TAGATACGGGTATCTTTACCGGCCGTTCGCCTAAAGATAAGTATATCGTATATGATGAAACATCAAAAGATAATGTTTGG
TGGACATCGGATGCGGTTAAAAATGACAATAAACCGATGAATCAAGCAACTTGGCAAAGTTTAAAAGAGCTGGTAACTCA
CCAATTGTCAAACAAACGTTTATTTGTAGTGGATGCATTCTGCGGTGCGAATAAAGACAGTCGCGTAGCGGTACGTATTG
TCACGGAAGTGGCGTGGCAAGCACACTTTGTGAAAAATATGTTCGTCCGTCCGTCGGAAGAAGAATTGTTGAATTTTGTG
CCTGATTTCGTTGTAATGAACGGCTCGAAAGTAACTAACCCGAATTGGAAAGAGCAAGGTTTAAATTCCGAAAACTTCGT
TGCGTTTAACTTAACTGAAAAAATCCAACTTATCGGCGGTACTTGGTACGGTGGCGAAATGAAGAAAGGTTTATTCTCTC
TGATGAACTACTGGTTACCGCTTAAAGGCATTGCGTCAATGCACTGTTCTGCAAACGTGGGGGCGGAAGGTGATGTAGCG
GTATTCTTCGGTTTATCCGGTACGGGTAAAACAACGCTTTCGACCGATCCTAAACGTAAATTAATCGGTGACGACGAACA
CGGTTGGGATGATGACGGCGTATTCAACTACGAAGGCGGTTGTTATGCGAAAACCATCAATCTTTCCGAAGAAAACGAGC
CGGATATCTATCGTGCAATCCGTCGTGATGCGTTATTAGAAAACGTTGTGGTGCGTGAAGACGGTTCGGTTGATTTTGCG
GACGGTTCAAAAACCGAAAATACGCGCGTATCTTATCCGATCCATCATATCGATAATATCGTTGAGCCGGTTTCAAAAGC
GGGTCATGCGAAAAAAGTGATTTTCTTAACCGCAGATGCGTTCGGCGTGTTACCTCCAGTTTCTAAATTAACGCCGGAAC
AAACCAAATATTACTTCTTATCCGGTTTTACCGCTAAATTAGCGGGTACCGAACGTGGCATTACCGAGCCTACACCGACT
TTCTCAGCGTGTTTCGGTGCGGCATTCTTATCACTTCACCCGACCAAATATGCGGAAGTATTAGTCAAACGTATGGAAGA
AGCGGGGTCGCAAGCATACTTAGTAAATACCGGTTGGAACGGTTCGGGTAAACGTATTTCGATTAAAGATACTCGCGGTA
TTATCGATGCGATTTTAGACGGTTCAATTGAAAAAGCGGAAACAAAAGAATTACCGATTTTCAATTTAGCGATTCCGACC
GCATTACCGAACGTAGATCCTGCGATCCTTGACCCTCGCGATACTTATGCGGATAAAGCGCAATGGCAAACTAAAGCGGA
AGATTTAGCGGGTCGTTTTGTGAAGAACTTTGAAAAATATACTACAAATGATGAAGGTAAAGCGTTAGTTGCGGCAGGTC
CGAAACTTTAA

Upstream 100 bases:

>100_bases
TAAAAAATTTGAAGTACTTAACATTTTTTTTAGTAAGAATTGGTTACAATGAACTTAGTTTTTAAATTTCAACTTTTAAT
ATTATGAATGAGGTAATTCT

Downstream 100 bases:

>100_bases
TACTAAATCCAAAGTTTGTTGAGTAATATTTGAAAAGGCTAATTAGCCGATAATAATCTGCACCTAATAGATTCATATCC
ATTTAGGTGCAGATTTTTTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MLSRIEQELAQLGITNVKEIVRNPSYEQLFEEEMKPELEGFEKGRLTTSGAVAVDTGIFTGRSPKDKYIVYDETSKDNVW
WTSDAVKNDNKPMNQATWQSLKELVTHQLSNKRLFVVDAFCGANKDSRVAVRIVTEVAWQAHFVKNMFVRPSEEELLNFV
PDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSLMNYWLPLKGIASMHCSANVGAEGDVA
VFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNYEGGCYAKTINLSEENEPDIYRAIRRDALLENVVVREDGSVDFA
DGSKTENTRVSYPIHHIDNIVEPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPT
FSACFGAAFLSLHPTKYAEVLVKRMEEAGSQAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIEKAETKELPIFNLAIPT
ALPNVDPAILDPRDTYADKAQWQTKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKL

Sequences:

>Translated_536_residues
MLSRIEQELAQLGITNVKEIVRNPSYEQLFEEEMKPELEGFEKGRLTTSGAVAVDTGIFTGRSPKDKYIVYDETSKDNVW
WTSDAVKNDNKPMNQATWQSLKELVTHQLSNKRLFVVDAFCGANKDSRVAVRIVTEVAWQAHFVKNMFVRPSEEELLNFV
PDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSLMNYWLPLKGIASMHCSANVGAEGDVA
VFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNYEGGCYAKTINLSEENEPDIYRAIRRDALLENVVVREDGSVDFA
DGSKTENTRVSYPIHHIDNIVEPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPT
FSACFGAAFLSLHPTKYAEVLVKRMEEAGSQAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIEKAETKELPIFNLAIPT
ALPNVDPAILDPRDTYADKAQWQTKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKL
>Mature_536_residues
MLSRIEQELAQLGITNVKEIVRNPSYEQLFEEEMKPELEGFEKGRLTTSGAVAVDTGIFTGRSPKDKYIVYDETSKDNVW
WTSDAVKNDNKPMNQATWQSLKELVTHQLSNKRLFVVDAFCGANKDSRVAVRIVTEVAWQAHFVKNMFVRPSEEELLNFV
PDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSLMNYWLPLKGIASMHCSANVGAEGDVA
VFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNYEGGCYAKTINLSEENEPDIYRAIRRDALLENVVVREDGSVDFA
DGSKTENTRVSYPIHHIDNIVEPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPT
FSACFGAAFLSLHPTKYAEVLVKRMEEAGSQAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIEKAETKELPIFNLAIPT
ALPNVDPAILDPRDTYADKAQWQTKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=531, Percent_Identity=78.3427495291902, Blast_Score=876, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=547, Percent_Identity=45.8866544789762, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_ACTP2 (A3N0G0)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053501.1
- ProteinModelPortal:   A3N0G0
- SMR:   A3N0G0
- STRING:   A3N0G0
- GeneID:   4848958
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0800
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- PhylomeDB:   A3N0G0
- ProtClustDB:   PRK09344
- BioCyc:   APLE416269:APL_0800-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59428; Mature: 59428

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRIEQELAQLGITNVKEIVRNPSYEQLFEEEMKPELEGFEKGRLTTSGAVAVDTGIFT
CHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCEECCCCEEEECCEEC
GRSPKDKYIVYDETSKDNVWWTSDAVKNDNKPMNQATWQSLKELVTHQLSNKRLFVVDAF
CCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
CGANKDSRVAVRIVTEVAWQAHFVKNMFVRPSEEELLNFVPDFVVMNGSKVTNPNWKEQG
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCEECCCCHHHHC
LNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSLMNYWLPLKGIASMHCSANVGAEGDVA
CCCCCEEEEEECCEEEEEECEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEE
VFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNYEGGCYAKTINLSEENEPDIYRAI
EEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHH
RRDALLENVVVREDGSVDFADGSKTENTRVSYPIHHIDNIVEPVSKAGHAKKVIFLTADA
HHHHHHHHHEEECCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCC
FGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYAEV
CCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
LVKRMEEAGSQAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIEKAETKELPIFNLAIPT
HHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
ALPNVDPAILDPRDTYADKAQWQTKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKL
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure
MLSRIEQELAQLGITNVKEIVRNPSYEQLFEEEMKPELEGFEKGRLTTSGAVAVDTGIFT
CHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCEECCCCEEEECCEEC
GRSPKDKYIVYDETSKDNVWWTSDAVKNDNKPMNQATWQSLKELVTHQLSNKRLFVVDAF
CCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
CGANKDSRVAVRIVTEVAWQAHFVKNMFVRPSEEELLNFVPDFVVMNGSKVTNPNWKEQG
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCEECCCCHHHHC
LNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSLMNYWLPLKGIASMHCSANVGAEGDVA
CCCCCEEEEEECCEEEEEECEEECCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEE
VFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNYEGGCYAKTINLSEENEPDIYRAI
EEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHH
RRDALLENVVVREDGSVDFADGSKTENTRVSYPIHHIDNIVEPVSKAGHAKKVIFLTADA
HHHHHHHHHEEECCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCC
FGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYAEV
CCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
LVKRMEEAGSQAYLVNTGWNGSGKRISIKDTRGIIDAILDGSIEKAETKELPIFNLAIPT
HHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
ALPNVDPAILDPRDTYADKAQWQTKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKL
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA