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The map label for this gene is pyrD

Identifier: 126208252

GI number: 126208252

Start: 890441

End: 891448

Strand: Reverse

Name: pyrD

Synonym: APL_0774

Alternate gene names: 126208252

Gene position: 891448-890441 (Counterclockwise)

Preceding gene: 126208253

Following gene: 126208251

Centisome position: 39.19

GC content: 42.46

Gene sequence:

>1008_bases
ATGTATTCTCTGATTCGTAAATGTCTTTTTTCAATGGATGCTGAAACTGCACATAACTTTTCTATTCAAGCACTTAAATT
AGCCGGAAAACTGCCGATTAATGTTTTGCCTATGCCGTTAAATCCCGTAGAAGTTATGGGGTTACAATTTAAAAATCCGA
TCGGTTTGGCTGCCGGCGCGGATAAAAACGGTGAAGCGATAGACGGTTTCGGAAAGTTGGGATTCGGATTTATTGAAGTC
GGTACGGTAACGCCGGTTGCGCAAGACGGTAATCCTAAGCCGCGCCAGTTCCGTATTTTAGAAGCGGAAGGCATTATTAA
CCGTAACGGTTTTAATAATTTAGGTGTGGACGTATTGGTCGAAAATGTTAAGAAAGCAAAATATGACGGCATTATCGGCA
TTAATATCGGTAAAAATGCGGTCACACCGATAGAACGTGCATTAGACGATTATCAAATTTGTTTACGTAAGGTATATGAA
CATGCGGATTATATTACGGTTAATATTTCTTCACCGAATACTAAAAATTTACGCACTTTGCAGTATGGCGAAGCGTTAGA
TGATCTGTTACGCTCGTTGAAAAGCGAGCAGGAAAGTCTGTCGCAAAAATTTAACCGATATAAACCGCTTGTCTTAAAAA
TCGCACCGGATTTAACCGATGAAGAAATCGCTTCGGTAGCGGATAGTTTGGTTCGTTATAAAATTGACGGCGTGATTGCA
GGAAATACGACCCTGTCCCGAGATCCGGTAGTCGGATTAAAAAACGCCGAGCAACAAGGCGGTTTAAGCGGTAAACCGTT
GAATACGTTAAGTACGCGTTTGATTAGTACGTTAGCGAAAGAGTTAAACGGTGCCTTACCGATTATCGGTAGCGGCGGCA
TTCACTCCGTGGCGTCCGGTCAGGAAAAGATTGATGCGGGCGCGAGTTTGTTACAAGTTTACTCGGCAATGATTTATCAG
GGGCCGGCGTTAATTCAAAATCTCGCAAAGCATATTCAAGTGCGTTAA

Upstream 100 bases:

>100_bases
TCCATCTCTGCAATGTTCGATGCATAATATCCAGTAAGGGCGGGTTTGATACCCGCCCTTAATTTATCCGTTTCTTTCTA
ACTATATCCAAGAGTTCACC

Downstream 100 bases:

>100_bases
TAGTAAGCGGTTAAATATTTAATCGTTTTTGTGAAATGAAATTGCTTGTTTAATAGACTATTCCTTTGTTATTGACTAAA
GTCAAAAAAATGGACAAAAC

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MYSLIRKCLFSMDAETAHNFSIQALKLAGKLPINVLPMPLNPVEVMGLQFKNPIGLAAGADKNGEAIDGFGKLGFGFIEV
GTVTPVAQDGNPKPRQFRILEAEGIINRNGFNNLGVDVLVENVKKAKYDGIIGINIGKNAVTPIERALDDYQICLRKVYE
HADYITVNISSPNTKNLRTLQYGEALDDLLRSLKSEQESLSQKFNRYKPLVLKIAPDLTDEEIASVADSLVRYKIDGVIA
GNTTLSRDPVVGLKNAEQQGGLSGKPLNTLSTRLISTLAKELNGALPIIGSGGIHSVASGQEKIDAGASLLQVYSAMIYQ
GPALIQNLAKHIQVR

Sequences:

>Translated_335_residues
MYSLIRKCLFSMDAETAHNFSIQALKLAGKLPINVLPMPLNPVEVMGLQFKNPIGLAAGADKNGEAIDGFGKLGFGFIEV
GTVTPVAQDGNPKPRQFRILEAEGIINRNGFNNLGVDVLVENVKKAKYDGIIGINIGKNAVTPIERALDDYQICLRKVYE
HADYITVNISSPNTKNLRTLQYGEALDDLLRSLKSEQESLSQKFNRYKPLVLKIAPDLTDEEIASVADSLVRYKIDGVIA
GNTTLSRDPVVGLKNAEQQGGLSGKPLNTLSTRLISTLAKELNGALPIIGSGGIHSVASGQEKIDAGASLLQVYSAMIYQ
GPALIQNLAKHIQVR
>Mature_335_residues
MYSLIRKCLFSMDAETAHNFSIQALKLAGKLPINVLPMPLNPVEVMGLQFKNPIGLAAGADKNGEAIDGFGKLGFGFIEV
GTVTPVAQDGNPKPRQFRILEAEGIINRNGFNNLGVDVLVENVKKAKYDGIIGINIGKNAVTPIERALDDYQICLRKVYE
HADYITVNISSPNTKNLRTLQYGEALDDLLRSLKSEQESLSQKFNRYKPLVLKIAPDLTDEEIASVADSLVRYKIDGVIA
GNTTLSRDPVVGLKNAEQQGGLSGKPLNTLSTRLISTLAKELNGALPIIGSGGIHSVASGQEKIDAGASLLQVYSAMIYQ
GPALIQNLAKHIQVR

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=334, Percent_Identity=42.5149700598802, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI119943098, Length=334, Percent_Identity=26.6467065868263, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787177, Length=334, Percent_Identity=59.5808383233533, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17509475, Length=333, Percent_Identity=41.1411411411411, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI281361352, Length=326, Percent_Identity=42.638036809816, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI17137316, Length=326, Percent_Identity=42.638036809816, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_ACTP2 (A3N0D6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053477.1
- ProteinModelPortal:   A3N0D6
- SMR:   A3N0D6
- STRING:   A3N0D6
- GeneID:   4849059
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0774
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- PhylomeDB:   A3N0D6
- ProtClustDB:   PRK05286
- BioCyc:   APLE416269:APL_0774-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36192; Mature: 36192

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLIRKCLFSMDAETAHNFSIQALKLAGKLPINVLPMPLNPVEVMGLQFKNPIGLAAGA
CHHHHHHHHHHCCCCHHCCCEEEEEEECCCCCCEEECCCCCHHHHHCEEECCCCCEECCC
DKNGEAIDGFGKLGFGFIEVGTVTPVAQDGNPKPRQFRILEAEGIINRNGFNNLGVDVLV
CCCCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
ENVKKAKYDGIIGINIGKNAVTPIERALDDYQICLRKVYEHADYITVNISSPNTKNLRTL
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
QYGEALDDLLRSLKSEQESLSQKFNRYKPLVLKIAPDLTDEEIASVADSLVRYKIDGVIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEE
GNTTLSRDPVVGLKNAEQQGGLSGKPLNTLSTRLISTLAKELNGALPIIGSGGIHSVASG
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCC
QEKIDAGASLLQVYSAMIYQGPALIQNLAKHIQVR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYSLIRKCLFSMDAETAHNFSIQALKLAGKLPINVLPMPLNPVEVMGLQFKNPIGLAAGA
CHHHHHHHHHHCCCCHHCCCEEEEEEECCCCCCEEECCCCCHHHHHCEEECCCCCEECCC
DKNGEAIDGFGKLGFGFIEVGTVTPVAQDGNPKPRQFRILEAEGIINRNGFNNLGVDVLV
CCCCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
ENVKKAKYDGIIGINIGKNAVTPIERALDDYQICLRKVYEHADYITVNISSPNTKNLRTL
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
QYGEALDDLLRSLKSEQESLSQKFNRYKPLVLKIAPDLTDEEIASVADSLVRYKIDGVIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEE
GNTTLSRDPVVGLKNAEQQGGLSGKPLNTLSTRLISTLAKELNGALPIIGSGGIHSVASG
CCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCC
QEKIDAGASLLQVYSAMIYQGPALIQNLAKHIQVR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA