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The map label for this gene is lpdA [H]

Identifier: 126208249

GI number: 126208249

Start: 883954

End: 885378

Strand: Reverse

Name: lpdA [H]

Synonym: APL_0771

Alternate gene names: 126208249

Gene position: 885378-883954 (Counterclockwise)

Preceding gene: 126208250

Following gene: 126208245

Centisome position: 38.93

GC content: 43.44

Gene sequence:

>1425_bases
ATGAGCAAAGAAATCAAAACGCAAGTCGTGGTACTTGGTGCGGGTCCTGCCGGTTATTCAGCGGCATTCCGTTGTGCCGA
CTTAGGCTTAGAAACAGTAATTGTCGAACGTTATTCAACTTTGGGCGGTGTATGCTTAAACGTAGGTTGTATTCCGTCTA
AAGCATTATTACACGTTGCAAAAGTTATCGAAGAAGCAAAACACGCAGAGAAAAACGGTATTACTTTCGGTGAGCCCAAC
ATTGATTTAGATAAAGTGCGTGCGGGTAAAGAAGCGGTTGTTTCTAAATTAACCGGCGGTTTAGCGGGTATGGCTAAAGC
ACGTAAAGTAACAGTAGTGGAAGGTTTAGCGGCGTTTACCGATCCGAATACTTTAGTAGCTCGTGACCGTGACGGTAATC
CGACAACGATTAAATTTGATAATGCAATTATTGCAGCCGGTTCTCGTCCGATTCAGCTTCCGTTCATTCCACACGAAGAT
CCGCGTGTGTGGGATTCTACGGATGCACTTAAATTAAAAGAAGTACCGAAAAAATTACTCATTATGGGCGGTGGTATCAT
CGGTTTAGAAATGGGTACCGTGTATAACGCTTTAGGTTCGGAAGTTGAAGTGGTTGAGATGTTTGACCAAGTGATTCCTG
CAGCAGATAAAGATGTTGTAGCTATCTACACCAAACAAATCGAGAAAAAATTCAAGTTAATGCTTGAAACTAAAGTAACT
GCGGTTGAAGCGAAAGATGACGGTATCTATGTTTCAATGGAAGGTAAAGCATGTAACGATACTAAACGTTACGATGCGGT
ATTAGTCGCTATCGGTCGTGTACCGAACGGTAAATTAATCGATGCGGGTAAAGCGGGCGTGAATGTTGATGATCGCGGTT
TCATCGCAGTGGATAAACAAATGCGTACCAACGTACCGCATATCTTTGCTATCGGTGATATCGTCGGTCAGCCGATGTTA
GCACACAAAGGTGTTCACGAAGGTCATGTAGCGGCAGAAGTGATTGCCGGTCAAAAACACTATTTTGATCCTAAAGTGAT
TCCTTCAATCGCTTATACAGAGCCGGAAGTTGCTTGGGTAGGTAAAACGGAGAAAGAGTGTAAGCAAGAAGGCTTAAACT
ACGAAGTGGCTAAATTCCCTTGGGCTGCATCAGGTCGTGCGATCGCTTCCGAATGTTCGGAAGGTATGACTAAACTTATC
TTCGATAAAGATACTCACCGTTTACTTGGCGGTGCAATCGTAGGTAGCAACGGCGGTGAATTATTAGGTGAAATCGGTCT
TGCAATCGAAATGGGTTGCGATGCGGAAGATATCGCTTTAACTATCCATGCTCATCCTACATTACACGAATCGGTAGGTT
TAGCTGCGGAAGTATTTGAAGGCTCTATTACAGACTTACCAAATGCAAAAGCGAAAAAACGTTAA

Upstream 100 bases:

>100_bases
TGTAATAGGTAAGCGGTTGAATTTACAAAAAATGTTGTAAAATCGACCGCTTGTATCGTAGGGGGCGCAGTACTTGTGCC
CCTACAACAAAGGAATAAAT

Downstream 100 bases:

>100_bases
TAGGTTAGTTTAACTTATTTATGCAAGCGGTTGAAATTTGTAAAAAACAGCAAATTTCGACCGCTTTTCTATTTTAAATG
AGCAGATTGCTTGGCATAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHAEKNGITFGEPN
IDLDKVRAGKEAVVSKLTGGLAGMAKARKVTVVEGLAAFTDPNTLVARDRDGNPTTIKFDNAIIAAGSRPIQLPFIPHED
PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVAIYTKQIEKKFKLMLETKVT
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVNVDDRGFIAVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLI
FDKDTHRLLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKR

Sequences:

>Translated_474_residues
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHAEKNGITFGEPN
IDLDKVRAGKEAVVSKLTGGLAGMAKARKVTVVEGLAAFTDPNTLVARDRDGNPTTIKFDNAIIAAGSRPIQLPFIPHED
PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVAIYTKQIEKKFKLMLETKVT
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVNVDDRGFIAVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLI
FDKDTHRLLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKR
>Mature_473_residues
SKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHAEKNGITFGEPNI
DLDKVRAGKEAVVSKLTGGLAGMAKARKVTVVEGLAAFTDPNTLVARDRDGNPTTIKFDNAIIAAGSRPIQLPFIPHEDP
RVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVAIYTKQIEKKFKLMLETKVTA
VEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVNVDDRGFIAVDKQMRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIF
DKDTHRLLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKR

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=42.3580786026201, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI50301238, Length=450, Percent_Identity=27.3333333333333, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI22035672, Length=427, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI33519430, Length=436, Percent_Identity=26.1467889908257, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519428, Length=436, Percent_Identity=26.1467889908257, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519426, Length=436, Percent_Identity=26.1467889908257, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI148277065, Length=436, Percent_Identity=26.1467889908257, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI148277071, Length=436, Percent_Identity=26.1467889908257, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=27.8801843317972, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=26.5258215962441, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=82.5263157894737, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=27.4468085106383, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=28.4463894967177, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=26.7415730337079, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=42.6339285714286, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.9411764705882, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=23.8532110091743, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=23.8532110091743, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=24.3119266055046, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=146, Percent_Identity=30.8219178082192, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.8859649122807, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.991452991453, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=27.7533039647577, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21358499, Length=450, Percent_Identity=42.2222222222222, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=29.2993630573248, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=29.2993630573248, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=29.2993630573248, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17737741, Length=468, Percent_Identity=26.0683760683761, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50553; Mature: 50422

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKHAEKNGITFGEPNIDLDKVRAGKEAVVSKLTGGLAGMAKARKVTVVEGLAAFT
HHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHC
DPNTLVARDRDGNPTTIKFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALKLKEVPKKLL
CCCEEEEECCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHCCCEEE
IMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVAIYTKQIEKKFKLMLETKVT
EECCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHEEEEE
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVNVDDRGFIAVDKQ
EEEECCCCEEEEECCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCCCCEEEC
GKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIFDKDTHRLLGGAIVGSNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEECCCHHHHHCCEEECCCCCH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKR
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKHAEKNGITFGEPNIDLDKVRAGKEAVVSKLTGGLAGMAKARKVTVVEGLAAFT
HHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHC
DPNTLVARDRDGNPTTIKFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALKLKEVPKKLL
CCCEEEEECCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHCCCEEE
IMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVAIYTKQIEKKFKLMLETKVT
EECCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHEEEEE
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVNVDDRGFIAVDKQ
EEEECCCCEEEEECCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCCCCCCCEEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCCCCEEEC
GKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIFDKDTHRLLGGAIVGSNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEECCCHHHHHCCEEECCCCCH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKR
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]