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The map label for this gene is menE [H]

Identifier: 126208229

GI number: 126208229

Start: 861292

End: 862722

Strand: Reverse

Name: menE [H]

Synonym: APL_0751

Alternate gene names: 126208229

Gene position: 862722-861292 (Counterclockwise)

Preceding gene: 126208230

Following gene: 126208228

Centisome position: 37.93

GC content: 44.51

Gene sequence:

>1431_bases
ATGGTAAATTTTTCCCTTTTTCCGACCGCCTATTGGGCGCAACATTTTGCACAACAACCGGCTATTATCTGGGAAAAAGG
GAATAGTTTATTATTTCCTGAACTTCCCGCTCGGCTAAACTGGCAACAGTTACAGTTACTCATTGCACAACTTGTCACTT
TCCTGCAACAACAAGGCGTACGGCAAAACGCATTAGTCGCTTATTCGGGGTCGCACCGTTTAGTCGGATTATTATGTTAT
TTAGCCGCTATCGCAGTAGGCGCGCGCATTTTAATGCTTAATCCGTCATTAACGGAGTCGCAGCGACAAACCGTTTTGGC
GGAATACGGTATCAAGATACTTATTACCGATCATCATTTTGCAAATTTTTCATTAAAAACGACCGCTTCGGTTTTACCTC
AAATCGCTTTTTCGGCACCGGCGACGCTCACGCTTACTTCGGGATCATCGGGTTTACCTAAAGCGATTGTGCATAGTGTG
CAAAATCATTTGGATAATGCCGAAGGCGTGTGTGAGTTAATGCGATTTACGCAGCAAGATTCTTGGCTATTAAGCTTGCC
GCTTTTTCATGTTTCGGGGCAGGGAATCGTTTGGCGCTGGTTGGCGAAAGGTGCAACTTTGGCGGTAAATGAGCAGAAAG
AGCATTTTTTTGCTTGTGTCGATAAAGTTTCGCACGCCTCACTTGTTCCGACACAGTTACGCCGTTATTTGCAGCAAAAA
TCACAAAAGCGCGTGATGGGGACGAAACGTTTTTTACTCGGCGGAAGTGCGATTCCGGCGGAGTTGGTGGCGGAAGCCCG
ACAACAAGGCATCGTGAGTTATTGCGGTTACGGTATGACGGAAATGGCATCAACCATTTGTGCGGTTGAAAACGAATTGG
ATAATGTCGGCTATCCCCTTAAAGGGCGAGAAGTTAAACTGGTTGAAAATGAAATTTGGGTCAAAGGTAGCGGTTTAGCC
TTAGGCGCATTGCAAAAAAACGGTGAAATTCGACCGCTTGTGAACCAAGAAGGTTGGTTGCAAACTAAAGACCGAGGCGA
ATGGAATGCCGCCGGTAAATTAGTGGTTAAAGGTCGCTTGGATAATATGTTTATTTCCGGCGGAGAAAATATTCAGCCGG
AAGACGTCGAAAAAGTGATCTATCAATCCGGTTTGGTCAGTCAGGTATTCGTCTTACCGGTAGAAGATGCGGAATTCGGA
CAAAGACCGGTTGCGGTTTTACAATTTATTCAGCCCGATTTTGCAAAAAACCGTGAAAATTTAACCGCTTGGCTTAGCGA
TAAGCTGGAAAAATTTAAACAACCGATCGCCTATTATCCGCTTGAACTTGTGCAATCTCAGCCGCAAGGCGGTATAAAAA
TCTCTCGTGCAAACTTACAATCCGTATTACATCAATTACTAGGAAATCATAATGATAAAGCGTCTGTTTAA

Upstream 100 bases:

>100_bases
AGGGCTTGATTCTTACCGGCGTAATGGAATCAATGGAACTTCCTCAACATATCATTGAGCGTGTTCGCACTATTTTTGCT
TAATTAATTTCTATCTGCTT

Downstream 100 bases:

>100_bases
CCCGTTCTTACTCGGCTTAATGTTCGTATTAAGCGGTAATCATCTTTGGGCGGCAGAACTGCCGACCGAAAAAGATTTGA
AAGCACAAATTGATGCCGCT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MVNFSLFPTAYWAQHFAQQPAIIWEKGNSLLFPELPARLNWQQLQLLIAQLVTFLQQQGVRQNALVAYSGSHRLVGLLCY
LAAIAVGARILMLNPSLTESQRQTVLAEYGIKILITDHHFANFSLKTTASVLPQIAFSAPATLTLTSGSSGLPKAIVHSV
QNHLDNAEGVCELMRFTQQDSWLLSLPLFHVSGQGIVWRWLAKGATLAVNEQKEHFFACVDKVSHASLVPTQLRRYLQQK
SQKRVMGTKRFLLGGSAIPAELVAEARQQGIVSYCGYGMTEMASTICAVENELDNVGYPLKGREVKLVENEIWVKGSGLA
LGALQKNGEIRPLVNQEGWLQTKDRGEWNAAGKLVVKGRLDNMFISGGENIQPEDVEKVIYQSGLVSQVFVLPVEDAEFG
QRPVAVLQFIQPDFAKNRENLTAWLSDKLEKFKQPIAYYPLELVQSQPQGGIKISRANLQSVLHQLLGNHNDKASV

Sequences:

>Translated_476_residues
MVNFSLFPTAYWAQHFAQQPAIIWEKGNSLLFPELPARLNWQQLQLLIAQLVTFLQQQGVRQNALVAYSGSHRLVGLLCY
LAAIAVGARILMLNPSLTESQRQTVLAEYGIKILITDHHFANFSLKTTASVLPQIAFSAPATLTLTSGSSGLPKAIVHSV
QNHLDNAEGVCELMRFTQQDSWLLSLPLFHVSGQGIVWRWLAKGATLAVNEQKEHFFACVDKVSHASLVPTQLRRYLQQK
SQKRVMGTKRFLLGGSAIPAELVAEARQQGIVSYCGYGMTEMASTICAVENELDNVGYPLKGREVKLVENEIWVKGSGLA
LGALQKNGEIRPLVNQEGWLQTKDRGEWNAAGKLVVKGRLDNMFISGGENIQPEDVEKVIYQSGLVSQVFVLPVEDAEFG
QRPVAVLQFIQPDFAKNRENLTAWLSDKLEKFKQPIAYYPLELVQSQPQGGIKISRANLQSVLHQLLGNHNDKASV
>Mature_476_residues
MVNFSLFPTAYWAQHFAQQPAIIWEKGNSLLFPELPARLNWQQLQLLIAQLVTFLQQQGVRQNALVAYSGSHRLVGLLCY
LAAIAVGARILMLNPSLTESQRQTVLAEYGIKILITDHHFANFSLKTTASVLPQIAFSAPATLTLTSGSSGLPKAIVHSV
QNHLDNAEGVCELMRFTQQDSWLLSLPLFHVSGQGIVWRWLAKGATLAVNEQKEHFFACVDKVSHASLVPTQLRRYLQQK
SQKRVMGTKRFLLGGSAIPAELVAEARQQGIVSYCGYGMTEMASTICAVENELDNVGYPLKGREVKLVENEIWVKGSGLA
LGALQKNGEIRPLVNQEGWLQTKDRGEWNAAGKLVVKGRLDNMFISGGENIQPEDVEKVIYQSGLVSQVFVLPVEDAEFG
QRPVAVLQFIQPDFAKNRENLTAWLSDKLEKFKQPIAYYPLELVQSQPQGGIKISRANLQSVLHQLLGNHNDKASV

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=343, Percent_Identity=24.198250728863, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI40807491, Length=318, Percent_Identity=23.8993710691824, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788595, Length=465, Percent_Identity=42.7956989247312, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI145693145, Length=345, Percent_Identity=25.5072463768116, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=410, Percent_Identity=26.5853658536585, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=437, Percent_Identity=23.5697940503432, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=362, Percent_Identity=21.5469613259669, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557194, Length=382, Percent_Identity=24.0837696335079, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538037, Length=374, Percent_Identity=23.7967914438503, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581924, Length=358, Percent_Identity=26.2569832402235, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52776; Mature: 52776

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFSLFPTAYWAQHFAQQPAIIWEKGNSLLFPELPARLNWQQLQLLIAQLVTFLQQQGV
CCCEEECCHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
RQNALVAYSGSHRLVGLLCYLAAIAVGARILMLNPSLTESQRQTVLAEYGIKILITDHHF
CCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCC
ANFSLKTTASVLPQIAFSAPATLTLTSGSSGLPKAIVHSVQNHLDNAEGVCELMRFTQQD
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SWLLSLPLFHVSGQGIVWRWLAKGATLAVNEQKEHFFACVDKVSHASLVPTQLRRYLQQK
CEEEEEEEEEECCCCEEEEEHHCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SQKRVMGTKRFLLGGSAIPAELVAEARQQGIVSYCGYGMTEMASTICAVENELDNVGYPL
HHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
KGREVKLVENEIWVKGSGLALGALQKNGEIRPLVNQEGWLQTKDRGEWNAAGKLVVKGRL
CCCEEEEEECEEEEEECCEEEEEECCCCCCCEECCCCCCEECCCCCCCCCCCEEEEEECC
DNMFISGGENIQPEDVEKVIYQSGLVSQVFVLPVEDAEFGQRPVAVLQFIQPDFAKNREN
CCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCHHHCCHHH
LTAWLSDKLEKFKQPIAYYPLELVQSQPQGGIKISRANLQSVLHQLLGNHNDKASV
HHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVNFSLFPTAYWAQHFAQQPAIIWEKGNSLLFPELPARLNWQQLQLLIAQLVTFLQQQGV
CCCEEECCHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
RQNALVAYSGSHRLVGLLCYLAAIAVGARILMLNPSLTESQRQTVLAEYGIKILITDHHF
CCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCC
ANFSLKTTASVLPQIAFSAPATLTLTSGSSGLPKAIVHSVQNHLDNAEGVCELMRFTQQD
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
SWLLSLPLFHVSGQGIVWRWLAKGATLAVNEQKEHFFACVDKVSHASLVPTQLRRYLQQK
CEEEEEEEEEECCCCEEEEEHHCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SQKRVMGTKRFLLGGSAIPAELVAEARQQGIVSYCGYGMTEMASTICAVENELDNVGYPL
HHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
KGREVKLVENEIWVKGSGLALGALQKNGEIRPLVNQEGWLQTKDRGEWNAAGKLVVKGRL
CCCEEEEEECEEEEEECCEEEEEECCCCCCCEECCCCCCEECCCCCCCCCCCEEEEEECC
DNMFISGGENIQPEDVEKVIYQSGLVSQVFVLPVEDAEFGQRPVAVLQFIQPDFAKNREN
CCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCHHHCCHHH
LTAWLSDKLEKFKQPIAYYPLELVQSQPQGGIKISRANLQSVLHQLLGNHNDKASV
HHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]