Click here to switch to the map view.

The map label for this gene is mepA [H]

Identifier: 126208225

GI number: 126208225

Start: 852612

End: 853442

Strand: Reverse

Name: mepA [H]

Synonym: APL_0747

Alternate gene names: 126208225

Gene position: 853442-852612 (Counterclockwise)

Preceding gene: 126208226

Following gene: 126208224

Centisome position: 37.52

GC content: 49.1

Gene sequence:

>831_bases
ATGAAAAGATTTAAAATGCTTTTGCTATCAAGTTTTCTGGGGTTGGTCACTTCTGCTCATGCGACAGTTTGGGAACAAAT
TCGTACTCCGGTTGCCGGACAAGCACAACCTATTGGCGGTTATAGTAACGGTTGTATTATCGGTGCGCAGCCGTTAGCGT
TAAAAGGAGACGGCTATCAAGTGATTCGTTCTGCTCGCAATCGTTTTTATGGGCATCCGGACTTGCTATCCTTTTTACAA
CGATTAGCCAAAAATACCAAAGCGGCAGGCTTACCGCCAATCTTAATCGGTGATATGGGGATGCCGGCGGGCGGGCGTTT
TTCTTCAGGTCATGCGAGTCATCAAACCGGCTTGGATGCGGATATTTGGTTTAGGTTCGGTCCGATGGACGAGCAAACGG
CGAAGAATCCGGCGGGTTTAGCCACTTTAATGGTGAATCGCGATGCACAGGTGGTTGATGAAAGCGTATGGACATCGCAA
CATGCGATGATGGTAAAACTGGCGGCACAAGATGCAAGAGTGGATAGAATTTTCGTGAATCCGGCGATTAAAGTGAAATT
ATGCAATACCGCCGGTTCGGATAGAAGCTGGCTGCGTAAAGTACGTCCTTGGTACGGACACGATTCGCATATGCACGTGC
GTTTAAGTTGTCCGCAAAACGCACCGAATTGTGAAAACCAAGCCCCGATTCCGGCGGGCGACGGCTGCGGTGCGGAGCTT
TATTCTTGGTTTGAGCCGGCACCGGCAGGTTCGACGGTATCAAAACCGAAAGTGTTACCGACACCACCGGTTCAATGTCA
GATGATTCTTTCGTCACAAGGACTGAACTAA

Upstream 100 bases:

>100_bases
CGTTAAGTCGGGTATAATCCGAACAAGCGGTCTTTTTTGCTAAAAATGCTGCAAAAAAGACCGCTTGTTTTTTTATTCAT
TGACTTTTCTAGGTGTAATA

Downstream 100 bases:

>100_bases
CAAAAAACGCACCTTAAATACTAAGGTGCGTTTTTAGTTTTTGAATCGCTATTCCGCTTTAATCGCAAATGCTTTAAGCA
TAGTGTGGAAAATATCGGTA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKRFKMLLLSSFLGLVTSAHATVWEQIRTPVAGQAQPIGGYSNGCIIGAQPLALKGDGYQVIRSARNRFYGHPDLLSFLQ
RLAKNTKAAGLPPILIGDMGMPAGGRFSSGHASHQTGLDADIWFRFGPMDEQTAKNPAGLATLMVNRDAQVVDESVWTSQ
HAMMVKLAAQDARVDRIFVNPAIKVKLCNTAGSDRSWLRKVRPWYGHDSHMHVRLSCPQNAPNCENQAPIPAGDGCGAEL
YSWFEPAPAGSTVSKPKVLPTPPVQCQMILSSQGLN

Sequences:

>Translated_276_residues
MKRFKMLLLSSFLGLVTSAHATVWEQIRTPVAGQAQPIGGYSNGCIIGAQPLALKGDGYQVIRSARNRFYGHPDLLSFLQ
RLAKNTKAAGLPPILIGDMGMPAGGRFSSGHASHQTGLDADIWFRFGPMDEQTAKNPAGLATLMVNRDAQVVDESVWTSQ
HAMMVKLAAQDARVDRIFVNPAIKVKLCNTAGSDRSWLRKVRPWYGHDSHMHVRLSCPQNAPNCENQAPIPAGDGCGAEL
YSWFEPAPAGSTVSKPKVLPTPPVQCQMILSSQGLN
>Mature_276_residues
MKRFKMLLLSSFLGLVTSAHATVWEQIRTPVAGQAQPIGGYSNGCIIGAQPLALKGDGYQVIRSARNRFYGHPDLLSFLQ
RLAKNTKAAGLPPILIGDMGMPAGGRFSSGHASHQTGLDADIWFRFGPMDEQTAKNPAGLATLMVNRDAQVVDESVWTSQ
HAMMVKLAAQDARVDRIFVNPAIKVKLCNTAGSDRSWLRKVRPWYGHDSHMHVRLSCPQNAPNCENQAPIPAGDGCGAEL
YSWFEPAPAGSTVSKPKVLPTPPVQCQMILSSQGLN

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=51.1904761904762, Blast_Score=270, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 29901; Mature: 29901

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFKMLLLSSFLGLVTSAHATVWEQIRTPVAGQAQPIGGYSNGCIIGAQPLALKGDGYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCHH
VIRSARNRFYGHPDLLSFLQRLAKNTKAAGLPPILIGDMGMPAGGRFSSGHASHQTGLDA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
DIWFRFGPMDEQTAKNPAGLATLMVNRDAQVVDESVWTSQHAMMVKLAAQDARVDRIFVN
EEEEEECCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHEEEEEHHCCCCEEEEEEC
PAIKVKLCNTAGSDRSWLRKVRPWYGHDSHMHVRLSCPQNAPNCENQAPIPAGDGCGAEL
CEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
YSWFEPAPAGSTVSKPKVLPTPPVQCQMILSSQGLN
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKRFKMLLLSSFLGLVTSAHATVWEQIRTPVAGQAQPIGGYSNGCIIGAQPLALKGDGYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCHH
VIRSARNRFYGHPDLLSFLQRLAKNTKAAGLPPILIGDMGMPAGGRFSSGHASHQTGLDA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
DIWFRFGPMDEQTAKNPAGLATLMVNRDAQVVDESVWTSQHAMMVKLAAQDARVDRIFVN
EEEEEECCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHEEEEEHHCCCCEEEEEEC
PAIKVKLCNTAGSDRSWLRKVRPWYGHDSHMHVRLSCPQNAPNCENQAPIPAGDGCGAEL
CEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
YSWFEPAPAGSTVSKPKVLPTPPVQCQMILSSQGLN
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]