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The map label for this gene is tmcA

Identifier: 126208175

GI number: 126208175

Start: 794137

End: 795927

Strand: Direct

Name: tmcA

Synonym: APL_0695

Alternate gene names: 126208175

Gene position: 794137-795927 (Clockwise)

Preceding gene: 126208174

Following gene: 126208177

Centisome position: 34.92

GC content: 44.44

Gene sequence:

>1791_bases
GTGATTTTAGCTGAAATGCCTCCGCTTGACGGGGGCATTGTTATTCATAGCGGCAATATCTCCCAAGCCAAACAGTATTT
GGGGCAAGAACACCCCTATGCGATTTACGATATGCGTGCCGAAGGCGATGTATGGCTTAACTTAGACGCATTAGCAATTA
TTGCCGGTACGATTCAGGCTCAAGGCACACTCTACCTTATTTGCCCGAACTGGGATACGCTCGAACAGCAACTAGATTTT
GATTCGCAACGTTGGAATGGCGGTAAGGTGATTGCCACACCGAATTTCTATCGCTACTTTAAGGCATTGGTACAAAAATT
CGGTTTTCAATTCCAAACATTGGAAGAAACGGATTTTAGCTTGCCAAAACAGCCGCCTCAATGCCAAGCGACCGAAAGTC
TTACGCCACAACAGCAAAAAATCTTTGAAAAATTACCGCTTGATTCGTCAGCTATTCATTTAATTACCGCGCCTCGTGGG
CGAGGCAAATCGACCCTCGCCGGTAAATTAGCTCAGCAGTTAGCTAAAACGGAATCGGTACTGATTACTGCAAGAAGTCA
CTCGGTATTACCTAGTTTTTGGAAGTCTGTTGCGCAGCATATTCCTTTTTTTGCCCCCGATCATCTGTTACAAAAAATCG
CGGCAAACCAAATTGCAGCAAAGAGTTGGTTATTTATTGATGAATCGGCAAGTTTGCCGTTACCAATGTTACATCAGTTC
TGTGAGTATTTCGACAAAGTGGTCTTAACCACGACTACACATAATTATGAAGGTACCGGGCGAGGCTTTAGCCTGAAATT
CCCACAACAGCTTACCAAGCAGTATCGAGAATGGCGACTGACTAAGCCGCTTCGTTGGCATGAAAATGATCCGTTGGAAC
AATTTATCGATGAATTATTGATTATGTCACCACCAAGCGAAACCAATCAATATGCTGAATTTTATCATTTACTGGCGGAA
GCGCATTACAAAACGACACCAAGCGATTTACGCCGATTATTTGATGCTCAAGATCAGTTACTACATTCTTTCTCCGAACA
TCAACGCTTAGTCGGTGGCATTTGGGCTGTACCTGAAGGTGATTTGGAACCTGAATTAGCTGAAGCCATTTGGCGGGGAG
AACGCCGCCCACAAGGCAATTTAGTCGCACAATACCTCTGCTTCCAAGGCAACTTACTCGAAGCCTGTCAGCTACGTTCG
GTACGCATCTCACGTATTGCGGTACAACCTGAATTGCAAAAGCAAGGGATTGGCAAGCGGTTAATTTCTGACTTTATTTT
GCAAAAAATTCAGCAAACTCGACCGCTTGTAGATTATGTTTCGGTCAGCTTTGGGCTCTCGGAACCTCTGTTGCATTTTT
GGCAACAATGCGGTTTTCAGTTAGTGCAAATTACCCCAACTAAAGAAGCCAGTAGCGGCTATCACAGTGCGATGATGCTC
TACCCGATTTCAGCCGAAGGTCAGCGGTTTGTACAACAAGCTACTGCCAGATTTGAACGAGATTTAGCATTACAGCCGTT
TTATGCAGAATTGCAAAATATGTTGCCAATTCGACCGCTTGTGCAACTGCAAATGGACGAACAAGATTGGCGCAATATAG
AAGGTTTCGCATTAGCGCAACGTTCTCTGGCGGCAAGTTATGTCAGCCTAACACGTCTTTACCGGCAAGATCCTCGCAAT
CATGGTGTATTAGCCAATCTCTGGCAACAATTCGAGCGTATTCAAGGCAAAAAAGAATGGTTGGAAAACTTACGTAGCCT
ACTTGCTAATTACTTGCAATACACACGCTAA

Upstream 100 bases:

>100_bases
CGGAAGAAATGGAAGGTTTAGAAACCGAGATTATGCAAAGTTTAGGTTTTGAAGATCCGTATATTTCAGAAAAAGTGATT
GAAGAATAATGCGTAAACTG

Downstream 100 bases:

>100_bases
TTGGTTGCAAATCGCTATAAAAAAACCACCGCTTATTGCGGTGGTTTTTCATTTACGAATACATTATTTTTTACGTTCTA
GCCATTTACCGTCAAGATAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MILAEMPPLDGGIVIHSGNISQAKQYLGQEHPYAIYDMRAEGDVWLNLDALAIIAGTIQAQGTLYLICPNWDTLEQQLDF
DSQRWNGGKVIATPNFYRYFKALVQKFGFQFQTLEETDFSLPKQPPQCQATESLTPQQQKIFEKLPLDSSAIHLITAPRG
RGKSTLAGKLAQQLAKTESVLITARSHSVLPSFWKSVAQHIPFFAPDHLLQKIAANQIAAKSWLFIDESASLPLPMLHQF
CEYFDKVVLTTTTHNYEGTGRGFSLKFPQQLTKQYREWRLTKPLRWHENDPLEQFIDELLIMSPPSETNQYAEFYHLLAE
AHYKTTPSDLRRLFDAQDQLLHSFSEHQRLVGGIWAVPEGDLEPELAEAIWRGERRPQGNLVAQYLCFQGNLLEACQLRS
VRISRIAVQPELQKQGIGKRLISDFILQKIQQTRPLVDYVSVSFGLSEPLLHFWQQCGFQLVQITPTKEASSGYHSAMML
YPISAEGQRFVQQATARFERDLALQPFYAELQNMLPIRPLVQLQMDEQDWRNIEGFALAQRSLAASYVSLTRLYRQDPRN
HGVLANLWQQFERIQGKKEWLENLRSLLANYLQYTR

Sequences:

>Translated_596_residues
MILAEMPPLDGGIVIHSGNISQAKQYLGQEHPYAIYDMRAEGDVWLNLDALAIIAGTIQAQGTLYLICPNWDTLEQQLDF
DSQRWNGGKVIATPNFYRYFKALVQKFGFQFQTLEETDFSLPKQPPQCQATESLTPQQQKIFEKLPLDSSAIHLITAPRG
RGKSTLAGKLAQQLAKTESVLITARSHSVLPSFWKSVAQHIPFFAPDHLLQKIAANQIAAKSWLFIDESASLPLPMLHQF
CEYFDKVVLTTTTHNYEGTGRGFSLKFPQQLTKQYREWRLTKPLRWHENDPLEQFIDELLIMSPPSETNQYAEFYHLLAE
AHYKTTPSDLRRLFDAQDQLLHSFSEHQRLVGGIWAVPEGDLEPELAEAIWRGERRPQGNLVAQYLCFQGNLLEACQLRS
VRISRIAVQPELQKQGIGKRLISDFILQKIQQTRPLVDYVSVSFGLSEPLLHFWQQCGFQLVQITPTKEASSGYHSAMML
YPISAEGQRFVQQATARFERDLALQPFYAELQNMLPIRPLVQLQMDEQDWRNIEGFALAQRSLAASYVSLTRLYRQDPRN
HGVLANLWQQFERIQGKKEWLENLRSLLANYLQYTR
>Mature_596_residues
MILAEMPPLDGGIVIHSGNISQAKQYLGQEHPYAIYDMRAEGDVWLNLDALAIIAGTIQAQGTLYLICPNWDTLEQQLDF
DSQRWNGGKVIATPNFYRYFKALVQKFGFQFQTLEETDFSLPKQPPQCQATESLTPQQQKIFEKLPLDSSAIHLITAPRG
RGKSTLAGKLAQQLAKTESVLITARSHSVLPSFWKSVAQHIPFFAPDHLLQKIAANQIAAKSWLFIDESASLPLPMLHQF
CEYFDKVVLTTTTHNYEGTGRGFSLKFPQQLTKQYREWRLTKPLRWHENDPLEQFIDELLIMSPPSETNQYAEFYHLLAE
AHYKTTPSDLRRLFDAQDQLLHSFSEHQRLVGGIWAVPEGDLEPELAEAIWRGERRPQGNLVAQYLCFQGNLLEACQLRS
VRISRIAVQPELQKQGIGKRLISDFILQKIQQTRPLVDYVSVSFGLSEPLLHFWQQCGFQLVQITPTKEASSGYHSAMML
YPISAEGQRFVQQATARFERDLALQPFYAELQNMLPIRPLVQLQMDEQDWRNIEGFALAQRSLAASYVSLTRLYRQDPRN
HGVLANLWQQFERIQGKKEWLENLRSLLANYLQYTR

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP)

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI1788818, Length=560, Percent_Identity=37.1428571428571, Blast_Score=319, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17507621, Length=484, Percent_Identity=21.4876033057851, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640662, Length=386, Percent_Identity=26.4248704663212, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TMCA_ACTP2 (A3N059)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053400.1
- STRING:   A3N059
- GeneID:   4849978
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0695
- eggNOG:   COG1444
- HOGENOM:   HBG416637
- OMA:   LAHYRTS
- PhylomeDB:   A3N059
- ProtClustDB:   CLSK918938
- BioCyc:   APLE416269:APL_0695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01886
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR007807
- Gene3D:   G3DSA:3.40.630.30

Pfam domain/function: PF05127 DUF699; SSF55729 Acyl_CoA_acyltransferase

EC number: NA

Molecular weight: Translated: 68437; Mature: 68437

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS51186 GNAT

Important sites: BINDING 138-138 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAEMPPLDGGIVIHSGNISQAKQYLGQEHPYAIYDMRAEGDVWLNLDALAIIAGTIQA
CCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHEEC
QGTLYLICPNWDTLEQQLDFDSQRWNGGKVIATPNFYRYFKALVQKFGFQFQTLEETDFS
CCEEEEECCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCC
LPKQPPQCQATESLTPQQQKIFEKLPLDSSAIHLITAPRGRGKSTLAGKLAQQLAKTESV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCHHE
LITARSHSVLPSFWKSVAQHIPFFAPDHLLQKIAANQIAAKSWLFIDESASLPLPMLHQF
EEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
CEYFDKVVLTTTTHNYEGTGRGFSLKFPQQLTKQYREWRLTKPLRWHENDPLEQFIDELL
HHHHCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IMSPPSETNQYAEFYHLLAEAHYKTTPSDLRRLFDAQDQLLHSFSEHQRLVGGIWAVPEG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
DLEPELAEAIWRGERRPQGNLVAQYLCFQGNLLEACQLRSVRISRIAVQPELQKQGIGKR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCHHHCCHHHH
LISDFILQKIQQTRPLVDYVSVSFGLSEPLLHFWQQCGFQLVQITPTKEASSGYHSAMML
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEE
YPISAEGQRFVQQATARFERDLALQPFYAELQNMLPIRPLVQLQMDEQDWRNIEGFALAQ
EEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEECCHHHHCCCCHHHHHH
RSLAASYVSLTRLYRQDPRNHGVLANLWQQFERIQGKKEWLENLRSLLANYLQYTR
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILAEMPPLDGGIVIHSGNISQAKQYLGQEHPYAIYDMRAEGDVWLNLDALAIIAGTIQA
CCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHEEC
QGTLYLICPNWDTLEQQLDFDSQRWNGGKVIATPNFYRYFKALVQKFGFQFQTLEETDFS
CCEEEEECCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCC
LPKQPPQCQATESLTPQQQKIFEKLPLDSSAIHLITAPRGRGKSTLAGKLAQQLAKTESV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCHHE
LITARSHSVLPSFWKSVAQHIPFFAPDHLLQKIAANQIAAKSWLFIDESASLPLPMLHQF
EEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
CEYFDKVVLTTTTHNYEGTGRGFSLKFPQQLTKQYREWRLTKPLRWHENDPLEQFIDELL
HHHHCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IMSPPSETNQYAEFYHLLAEAHYKTTPSDLRRLFDAQDQLLHSFSEHQRLVGGIWAVPEG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
DLEPELAEAIWRGERRPQGNLVAQYLCFQGNLLEACQLRSVRISRIAVQPELQKQGIGKR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCHHHCCHHHH
LISDFILQKIQQTRPLVDYVSVSFGLSEPLLHFWQQCGFQLVQITPTKEASSGYHSAMML
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEE
YPISAEGQRFVQQATARFERDLALQPFYAELQNMLPIRPLVQLQMDEQDWRNIEGFALAQ
EEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHEECCHHHHCCCCHHHHHH
RSLAASYVSLTRLYRQDPRNHGVLANLWQQFERIQGKKEWLENLRSLLANYLQYTR
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA