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The map label for this gene is mtfA

Identifier: 126208165

GI number: 126208165

Start: 783146

End: 784237

Strand: Reverse

Name: mtfA

Synonym: APL_0685

Alternate gene names: 126208165

Gene position: 784237-783146 (Counterclockwise)

Preceding gene: 126208166

Following gene: 126208164

Centisome position: 34.48

GC content: 41.67

Gene sequence:

>1092_bases
ATGAATAAACTAGCATTATATTGCCGAGCGGGATTTGAAAAAGAAACCGCCGGTGAGATTACCGATAAAGCGGCACAACT
TGGCGTGTTTGGCTTTGTGAATTTAAAAGAAAATAGCGGTTATATTATTTTTGAATGTTATCAAGCAGGCGATGCTGATC
GCTTAGCCAGAGAATTAAAATTCGAACAACTGATTTTTGCCCGTCAAATGATTGTAGTCGGCGATATGTTACAAGACTTG
CCGGCAGAAGATCGTATTTCACCGATTGTAGCCCAATACCAAGCGTTAAATCCTCGCCACAGTTCGGATATTTTTGTCGA
AACGCCGGATACGAATGAAGCAAAAGAATTACTGACCTTTTGTCGTAAATTTACCGTACCGCTACGTAGTAGTTTGAAAA
AACAAGGTTGGCTAACCAAGTCTGAACGTGCAAAGGGTAGTATGGGTTTACATATTTTATTTGTGCGACCGGGTTGTTGC
TATGTCGGCTATGCCTATAACGATAATAAATCGCCGTTTTTTATGGGGATTCCCCGTTTAAAATTCCCGGCGGAAGCACC
GAGCCGTTCTACATTAAAATTGGAAGAAGCAATTCTTACTTTTATTCCGGAAGCGGAAGAGAAAAAACGTTTTACCGATG
AAATGACCGGGGTGGATTTAGGTGCTTGCCCGGGCGGTTGGACTTATCAACTGGTTAAACGTGGCGTATTCGTGTATGCG
GTGGATCATGGCAAAATGGCGGCAAGTCTGCACGAAACCGGACGTATTGAGCATTGTCCTGAAGACGGTTTTAAATTCCA
ACCGCTTAAACGTAAAACTATCGACTGGTTGGTGTGCGACATGGTGGAACAGCCGATGCGGATTAGTAAATTAATCGGTA
AATGGTTAATTAACGGTTGGTGCCGTGAAACGATTTTTAACTTGAAATTGCCGATGAAAAAACGTTATCAAGAAGTGCAA
CTATGTTTAGCGTATCTTGAAGAAGAACTCGAAAAACAAGGCTTTTGGTTTAAGATTCAGGCAAAACATCTCTATCACGA
TAGAGAAGAAATTACCGTACATATTGCGGTAATGGGACGTAAACCCCAATAA

Upstream 100 bases:

>100_bases
ATTATATCCATATTGTATTTTTAATCAATTATTTGTTTTTCCTATCAAAATAGTACGCAAAACGCTATACTAATCGCATT
TTTCATCCATTAGAAAACCT

Downstream 100 bases:

>100_bases
GCAAAATTAACATAAATAAGCGGTAAAATTTTTACAATATTTTGCAAATTTATGGCAAAATCAGACCGCTTATTTTAACA
AGAACAAGACGTTATAGAGG

Product: putative RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNKLALYCRAGFEKETAGEITDKAAQLGVFGFVNLKENSGYIIFECYQAGDADRLARELKFEQLIFARQMIVVGDMLQDL
PAEDRISPIVAQYQALNPRHSSDIFVETPDTNEAKELLTFCRKFTVPLRSSLKKQGWLTKSERAKGSMGLHILFVRPGCC
YVGYAYNDNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPEAEEKKRFTDEMTGVDLGACPGGWTYQLVKRGVFVYA
VDHGKMAASLHETGRIEHCPEDGFKFQPLKRKTIDWLVCDMVEQPMRISKLIGKWLINGWCRETIFNLKLPMKKRYQEVQ
LCLAYLEEELEKQGFWFKIQAKHLYHDREEITVHIAVMGRKPQ

Sequences:

>Translated_363_residues
MNKLALYCRAGFEKETAGEITDKAAQLGVFGFVNLKENSGYIIFECYQAGDADRLARELKFEQLIFARQMIVVGDMLQDL
PAEDRISPIVAQYQALNPRHSSDIFVETPDTNEAKELLTFCRKFTVPLRSSLKKQGWLTKSERAKGSMGLHILFVRPGCC
YVGYAYNDNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPEAEEKKRFTDEMTGVDLGACPGGWTYQLVKRGVFVYA
VDHGKMAASLHETGRIEHCPEDGFKFQPLKRKTIDWLVCDMVEQPMRISKLIGKWLINGWCRETIFNLKLPMKKRYQEVQ
LCLAYLEEELEKQGFWFKIQAKHLYHDREEITVHIAVMGRKPQ
>Mature_363_residues
MNKLALYCRAGFEKETAGEITDKAAQLGVFGFVNLKENSGYIIFECYQAGDADRLARELKFEQLIFARQMIVVGDMLQDL
PAEDRISPIVAQYQALNPRHSSDIFVETPDTNEAKELLTFCRKFTVPLRSSLKKQGWLTKSERAKGSMGLHILFVRPGCC
YVGYAYNDNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPEAEEKKRFTDEMTGVDLGACPGGWTYQLVKRGVFVYA
VDHGKMAASLHETGRIEHCPEDGFKFQPLKRKTIDWLVCDMVEQPMRISKLIGKWLINGWCRETIFNLKLPMKKRYQEVQ
LCLAYLEEELEKQGFWFKIQAKHLYHDREEITVHIAVMGRKPQ

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily

Homologues:

Organism=Escherichia coli, GI1789171, Length=366, Percent_Identity=59.5628415300546, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMM_ACTP2 (A3N049)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053390.1
- ProteinModelPortal:   A3N049
- SMR:   A3N049
- STRING:   A3N049
- GeneID:   4850086
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0685
- eggNOG:   COG2933
- HOGENOM:   HBG681972
- OMA:   LYCRPGF
- PhylomeDB:   A3N049
- ProtClustDB:   PRK11760
- BioCyc:   APLE416269:APL_0685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01551
- InterPro:   IPR011224
- InterPro:   IPR002877
- PIRSF:   PIRSF028774

Pfam domain/function: PF01728 FtsJ

EC number: NA

Molecular weight: Translated: 41698; Mature: 41698

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLALYCRAGFEKETAGEITDKAAQLGVFGFVNLKENSGYIIFECYQAGDADRLARELK
CCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCHHHHHHHHH
FEQLIFARQMIVVGDMLQDLPAEDRISPIVAQYQALNPRHSSDIFVETPDTNEAKELLTF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
CRKFTVPLRSSLKKQGWLTKSERAKGSMGLHILFVRPGCCYVGYAYNDNKSPFFMGIPRL
HHHHCCHHHHHHHHHCCCCCHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCC
KFPAEAPSRSTLKLEEAILTFIPEAEEKKRFTDEMTGVDLGACPGGWTYQLVKRGVFVYA
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEE
VDHGKMAASLHETGRIEHCPEDGFKFQPLKRKTIDWLVCDMVEQPMRISKLIGKWLINGW
ECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
CRETIFNLKLPMKKRYQEVQLCLAYLEEELEKQGFWFKIQAKHLYHDREEITVHIAVMGR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEEEEEECC
KPQ
CCC
>Mature Secondary Structure
MNKLALYCRAGFEKETAGEITDKAAQLGVFGFVNLKENSGYIIFECYQAGDADRLARELK
CCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCCHHHHHHHHH
FEQLIFARQMIVVGDMLQDLPAEDRISPIVAQYQALNPRHSSDIFVETPDTNEAKELLTF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
CRKFTVPLRSSLKKQGWLTKSERAKGSMGLHILFVRPGCCYVGYAYNDNKSPFFMGIPRL
HHHHCCHHHHHHHHHCCCCCHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCC
KFPAEAPSRSTLKLEEAILTFIPEAEEKKRFTDEMTGVDLGACPGGWTYQLVKRGVFVYA
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEE
VDHGKMAASLHETGRIEHCPEDGFKFQPLKRKTIDWLVCDMVEQPMRISKLIGKWLINGW
ECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
CRETIFNLKLPMKKRYQEVQLCLAYLEEELEKQGFWFKIQAKHLYHDREEITVHIAVMGR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEEEEEECC
KPQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA