Click here to switch to the map view.

The map label for this gene is glnS [H]

Identifier: 126208159

GI number: 126208159

Start: 774644

End: 776314

Strand: Direct

Name: glnS [H]

Synonym: APL_0679

Alternate gene names: 126208159

Gene position: 774644-776314 (Clockwise)

Preceding gene: 126208158

Following gene: 126208167

Centisome position: 34.06

GC content: 41.71

Gene sequence:

>1671_bases
ATGACAGAAGAAATTTTAGAAAATGCGCCGGAAACCCGTGCGAATTTTATTACCCATATTATTGATGAAGATTTAAAAAG
CGGTAAACACAATAACGTTTACACTCGTTTCCCACCTGAGCCGAACGGCTACTTACATATCGGTCACGCAAAATCTATCT
GCTTAAACTTCGGTATTGCCCAAGAATATAAAGGCTTATGTAACCTTCGTTTTGATGATACGAATCCGGTAAAAGAAGAT
GTTGAATACGTTGATTCAATCAAACAAGACGTAGAATGGTTAGGTTTTAAATGGGAAGGCGAACCGCGTTATGCATCTGA
CTATTTTGATCAACTTTACGGCTATGCGATTGAGTTAATCAACAAAGGTTTAGCTTATGTTGATGAGCTTTCGCCGGATG
AAATGCGTGAATATCGCGGCACATTAACCGAACCGGGCCAAAATAGTCCATACCGTGATCGTAGCGTAGAAGAAAACTTA
GCGTTATTTGAAAAAATGAAAAACGGCGAGTTTGAAGAAGGTAAAGCGTGTTTACGTGCCAAAATTGATATGGCATCACC
ATTTATCGTAATGCGTGACCCTGTCATCTATCGTGTCAAATTTGCTCACCATCACCAAACCGGTGATAAATGGTGCATCT
ATCCGATGTACGACTTCACTCACTGTATTTCGGATGCGATTGAGCGCATTACTCACTCGTTATGTACGCTTGAATTCCAA
GATAACCGCCGTTTATACGATTGGGTACTTGAAAATATTTCTATCGAGCGTCCATTACCGCACCAATACGAATTTTCTCG
TTTAAATTTAGAAGGCACTCTCACGTCTAAACGTAAATTATTAAAATTGGTGACAGACGGCACGGTGGACGGTTGGAATG
ACCCACGTATGCCGACTATTTCAGGCTTACGTCGTCGTGGTTATACCCCTGCTTCATTACGTGAATTCTGCCGCCGTATC
GGTGTGACCAAACAAGATAATGTGGTGGAATACTCTGCACTTGAAGCCTGTATTCGTGAAGATTTAAACGAAAACGCACC
ACGTGCAATGGCAGTAATCAACCCGGTTCGTGTCGTGATTGAAAACTTTGGCGATAAAGAAATCTTAAAAGCACCGAATC
ACCCGAATCGTCCGGAATTAGGCGAGCGCGATTTACCGTTTACGCGTGAACTTTATATCGATGAAGCGGACTTCCGTGAA
GAAGCAAATAAACAGTACAAACGTTTAGTATTAGGCAAAGAAGTTCGCTTGCGTAATGCTTATGTGATTAAAGCAGAGCG
TGTAGAAAAAGATGAAAACGGCAAAATTACTACGATTTTCTGTACTTACGATCCGGAAACTTTAGGTAAGAACCCGGCTG
ACGGCCGCAAAGTAAAAGGTGTGATTCACTGGGTATCTGCAGAAGATAATAAACTGGCTGAATTCCGTATTTACAATCGT
TTATTCACAGTAGCGAATCCGGGTGCAGAAGACGATATTAATACGGTATTAAATCCGGAATCACTGGTAATCAAACACGG
CTTCGTTGAGCCGAGCCTCGTTAATGCGAAAGCGGAACAAGGCTACCAATTCGAACGTGAAGGCTATTACTGCTTAGACA
GTAAAGACGGTTCGGCTGAAAATCTAGTGTTTAACCTAACCGTAAGTTTAAAAGAAGGCTTTACGGCTTAA

Upstream 100 bases:

>100_bases
TCGACCGCTTTTTTATTTCTCTCTGTTTGTCGCTTTATGAAATTTGCAATACGGTGTAGAATACTCTGTTATTTTTATTT
AAAGGCAACAGAGAACAATT

Downstream 100 bases:

>100_bases
TTTTGATAATAAGCGGTCAAAAACGGCCGCTTTTTTACACTATTCGAGGCAATATGAAAAAAGTAATTTTATTAATAGCA
AGCATTTTAGCAATAAGTGC

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTEEILENAPETRANFITHIIDEDLKSGKHNNVYTRFPPEPNGYLHIGHAKSICLNFGIAQEYKGLCNLRFDDTNPVKED
VEYVDSIKQDVEWLGFKWEGEPRYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGQNSPYRDRSVEENL
ALFEKMKNGEFEEGKACLRAKIDMASPFIVMRDPVIYRVKFAHHHQTGDKWCIYPMYDFTHCISDAIERITHSLCTLEFQ
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVTDGTVDGWNDPRMPTISGLRRRGYTPASLREFCRRI
GVTKQDNVVEYSALEACIREDLNENAPRAMAVINPVRVVIENFGDKEILKAPNHPNRPELGERDLPFTRELYIDEADFRE
EANKQYKRLVLGKEVRLRNAYVIKAERVEKDENGKITTIFCTYDPETLGKNPADGRKVKGVIHWVSAEDNKLAEFRIYNR
LFTVANPGAEDDINTVLNPESLVIKHGFVEPSLVNAKAEQGYQFEREGYYCLDSKDGSAENLVFNLTVSLKEGFTA

Sequences:

>Translated_556_residues
MTEEILENAPETRANFITHIIDEDLKSGKHNNVYTRFPPEPNGYLHIGHAKSICLNFGIAQEYKGLCNLRFDDTNPVKED
VEYVDSIKQDVEWLGFKWEGEPRYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGQNSPYRDRSVEENL
ALFEKMKNGEFEEGKACLRAKIDMASPFIVMRDPVIYRVKFAHHHQTGDKWCIYPMYDFTHCISDAIERITHSLCTLEFQ
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVTDGTVDGWNDPRMPTISGLRRRGYTPASLREFCRRI
GVTKQDNVVEYSALEACIREDLNENAPRAMAVINPVRVVIENFGDKEILKAPNHPNRPELGERDLPFTRELYIDEADFRE
EANKQYKRLVLGKEVRLRNAYVIKAERVEKDENGKITTIFCTYDPETLGKNPADGRKVKGVIHWVSAEDNKLAEFRIYNR
LFTVANPGAEDDINTVLNPESLVIKHGFVEPSLVNAKAEQGYQFEREGYYCLDSKDGSAENLVFNLTVSLKEGFTA
>Mature_555_residues
TEEILENAPETRANFITHIIDEDLKSGKHNNVYTRFPPEPNGYLHIGHAKSICLNFGIAQEYKGLCNLRFDDTNPVKEDV
EYVDSIKQDVEWLGFKWEGEPRYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGQNSPYRDRSVEENLA
LFEKMKNGEFEEGKACLRAKIDMASPFIVMRDPVIYRVKFAHHHQTGDKWCIYPMYDFTHCISDAIERITHSLCTLEFQD
NRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVTDGTVDGWNDPRMPTISGLRRRGYTPASLREFCRRIG
VTKQDNVVEYSALEACIREDLNENAPRAMAVINPVRVVIENFGDKEILKAPNHPNRPELGERDLPFTRELYIDEADFREE
ANKQYKRLVLGKEVRLRNAYVIKAERVEKDENGKITTIFCTYDPETLGKNPADGRKVKGVIHWVSAEDNKLAEFRIYNRL
FTVANPGAEDDINTVLNPESLVIKHGFVEPSLVNAKAEQGYQFEREGYYCLDSKDGSAENLVFNLTVSLKEGFTA

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=46.0377358490566, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI62241042, Length=532, Percent_Identity=33.8345864661654, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1786895, Length=544, Percent_Identity=76.8382352941177, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=303, Percent_Identity=22.4422442244224, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543332, Length=535, Percent_Identity=40.9345794392523, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=33.7811900191939, Blast_Score=262, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=41.0301953818828, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321192, Length=535, Percent_Identity=36.8224299065421, Blast_Score=312, Evalue=8e-86,
Organism=Drosophila melanogaster, GI17864488, Length=531, Percent_Identity=42.3728813559322, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24649466, Length=518, Percent_Identity=35.5212355212355, Blast_Score=281, Evalue=1e-75,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64146; Mature: 64015

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEILENAPETRANFITHIIDEDLKSGKHNNVYTRFPPEPNGYLHIGHAKSICLNFGIA
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHCCCCH
QEYKGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEPRYASDYFDQLYGYAIELI
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHH
NKGLAYVDELSPDEMREYRGTLTEPGQNSPYRDRSVEENLALFEKMKNGEFEEGKACLRA
HCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
KIDMASPFIVMRDPVIYRVKFAHHHQTGDKWCIYPMYDFTHCISDAIERITHSLCTLEFQ
HHCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEC
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVTDGTVDGWNDPRMPTI
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHH
SGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVINPVRVVI
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ENFGDKEILKAPNHPNRPELGERDLPFTRELYIDEADFREEANKQYKRLVLGKEVRLRNA
HCCCCHHHHCCCCCCCCCCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHCCCEEECCE
YVIKAERVEKDENGKITTIFCTYDPETLGKNPADGRKVKGVIHWVSAEDNKLAEFRIYNR
EEEEHHHCCCCCCCCEEEEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LFTVANPGAEDDINTVLNPESLVIKHGFVEPSLVNAKAEQGYQFEREGYYCLDSKDGSAE
HEEECCCCCCCCHHHHCCCCCEEEECCCCCCHHCCCHHHCCCEECCCCEEEEECCCCCCC
NLVFNLTVSLKEGFTA
EEEEEEEEECCCCCCC
>Mature Secondary Structure 
TEEILENAPETRANFITHIIDEDLKSGKHNNVYTRFPPEPNGYLHIGHAKSICLNFGIA
CHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHCCCCH
QEYKGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEPRYASDYFDQLYGYAIELI
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHH
NKGLAYVDELSPDEMREYRGTLTEPGQNSPYRDRSVEENLALFEKMKNGEFEEGKACLRA
HCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
KIDMASPFIVMRDPVIYRVKFAHHHQTGDKWCIYPMYDFTHCISDAIERITHSLCTLEFQ
HHCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEC
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVTDGTVDGWNDPRMPTI
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHH
SGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVINPVRVVI
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ENFGDKEILKAPNHPNRPELGERDLPFTRELYIDEADFREEANKQYKRLVLGKEVRLRNA
HCCCCHHHHCCCCCCCCCCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHHCCCEEECCE
YVIKAERVEKDENGKITTIFCTYDPETLGKNPADGRKVKGVIHWVSAEDNKLAEFRIYNR
EEEEHHHCCCCCCCCEEEEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LFTVANPGAEDDINTVLNPESLVIKHGFVEPSLVNAKAEQGYQFEREGYYCLDSKDGSAE
HEEECCCCCCCCHHHHCCCCCEEEECCCCCCHHCCCHHHCCCEECCCCEEEEECCCCCCC
NLVFNLTVSLKEGFTA
EEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA