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The map label for this gene is ansB [H]

Identifier: 126208140

GI number: 126208140

Start: 753391

End: 754356

Strand: Direct

Name: ansB [H]

Synonym: APL_0660

Alternate gene names: 126208140

Gene position: 753391-754356 (Clockwise)

Preceding gene: 126208139

Following gene: 126208141

Centisome position: 33.12

GC content: 42.86

Gene sequence:

>966_bases
ATGACAAAGAAACTTTTAATCCTACATACGGGCGGTACGATTTCGATGAGTGAAGGCGAAGACGGTAAAGTTTCGCCTTC
CGAAAAGAATCCGCTGCTTGCCGCATTAGAACGTTTAAATCACCCGGCTCAACTCAGCCAAGAATCTGTGCTTAATGTGC
CTTCGCCGCATATCACGTTACAGCATTGGTTATTATTAAAAACACGTATTGAAAAAGCGGTAAATGAAGAACAGTATGAC
GGTATCGTGATTACCCACGGTACGGATACGTTAGAAGAAACCGCTTATTTCCTTGACTTAGCGCTGAATGTAAATGTACC
GGTTGCAATTACCGGCGCAATGCGTTCAAGTAACGAATTGGGTTCGGACGGTTTAATTAACTTACAAAGTGCGATTTTGG
TTGCACTTTGTCCGGAAAGTCGAAATAAAGGCGTATTAGTGGTGATGAACGATGAAATTCATAACGCTAAATTTGTGACT
AAAACGCATACGACCAACGTAGCGACATTCCAAACGCCGACCTTCGGCCCTTGCGGATTAATTGCGAAAAACCGCGTACT
GTATTTCCAACAATTAACCGAATACGAACGCTTCCCGATACAAACCGTTACCCGTACTAACGTACAGCTGGTTAAAGCCT
ATGCCGGTATGGACAGTTTCTTATTGGAACAATTAGCTCATCACGGCTGTAACGGCGTGGTAATTGAAGCATTAGGTGCA
GGTAATTTACCACCGAGTTGTTTAGCCGGTTTAGATGCGCTATTACGTGCCGATATTCCTGTTGTATTAGTTTCTCGTGC
GTTTAACGGCGTTACTCAGGATGTGTACGACTATTTAGGCGGTGGTAAGCAGCTTAAACAACAAAATGTGATTTTTACCA
CCGGTTTAAGCGGTCAGAAAGCACGTATCAAATTGTTAGTCTTGCTAAATCAAAACTTGAGTAAACCGCTCGCCGAATAT
TTTTAA

Upstream 100 bases:

>100_bases
GGCGTTTAACTCTGAGTTAGCTCAAAAGCTACACGCATTCCGCAATGAACAAACGCAATCGGTATTAGATAATCCAGATC
CACGTAACTAATAGATTTTC

Downstream 100 bases:

>100_bases
TCTCTGATAAACACAGAAAGTTTGAGGTTTCCGAGCCGTACAAGCGGTCAAATTTACCCCAAAAATTGCAAATCGAGCCT
CAAACTTTCCGCAAACGGAA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTKKLLILHTGGTISMSEGEDGKVSPSEKNPLLAALERLNHPAQLSQESVLNVPSPHITLQHWLLLKTRIEKAVNEEQYD
GIVITHGTDTLEETAYFLDLALNVNVPVAITGAMRSSNELGSDGLINLQSAILVALCPESRNKGVLVVMNDEIHNAKFVT
KTHTTNVATFQTPTFGPCGLIAKNRVLYFQQLTEYERFPIQTVTRTNVQLVKAYAGMDSFLLEQLAHHGCNGVVIEALGA
GNLPPSCLAGLDALLRADIPVVLVSRAFNGVTQDVYDYLGGGKQLKQQNVIFTTGLSGQKARIKLLVLLNQNLSKPLAEY
F

Sequences:

>Translated_321_residues
MTKKLLILHTGGTISMSEGEDGKVSPSEKNPLLAALERLNHPAQLSQESVLNVPSPHITLQHWLLLKTRIEKAVNEEQYD
GIVITHGTDTLEETAYFLDLALNVNVPVAITGAMRSSNELGSDGLINLQSAILVALCPESRNKGVLVVMNDEIHNAKFVT
KTHTTNVATFQTPTFGPCGLIAKNRVLYFQQLTEYERFPIQTVTRTNVQLVKAYAGMDSFLLEQLAHHGCNGVVIEALGA
GNLPPSCLAGLDALLRADIPVVLVSRAFNGVTQDVYDYLGGGKQLKQQNVIFTTGLSGQKARIKLLVLLNQNLSKPLAEY
F
>Mature_320_residues
TKKLLILHTGGTISMSEGEDGKVSPSEKNPLLAALERLNHPAQLSQESVLNVPSPHITLQHWLLLKTRIEKAVNEEQYDG
IVITHGTDTLEETAYFLDLALNVNVPVAITGAMRSSNELGSDGLINLQSAILVALCPESRNKGVLVVMNDEIHNAKFVTK
THTTNVATFQTPTFGPCGLIAKNRVLYFQQLTEYERFPIQTVTRTNVQLVKAYAGMDSFLLEQLAHHGCNGVVIEALGAG
NLPPSCLAGLDALLRADIPVVLVSRAFNGVTQDVYDYLGGGKQLKQQNVIFTTGLSGQKARIKLLVLLNQNLSKPLAEYF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=278, Percent_Identity=27.3381294964029, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789327, Length=299, Percent_Identity=35.1170568561873, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788065, Length=326, Percent_Identity=26.3803680981595, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320527, Length=325, Percent_Identity=29.2307692307692, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323189, Length=298, Percent_Identity=29.5302013422819, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323187, Length=298, Percent_Identity=29.5302013422819, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323186, Length=298, Percent_Identity=29.5302013422819, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323184, Length=298, Percent_Identity=29.5302013422819, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645530, Length=259, Percent_Identity=28.1853281853282, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24639609, Length=350, Percent_Identity=24.5714285714286, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34982; Mature: 34851

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLLILHTGGTISMSEGEDGKVSPSEKNPLLAALERLNHPAQLSQESVLNVPSPHITL
CCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHCCHHHHCCCCCCCEEH
QHWLLLKTRIEKAVNEEQYDGIVITHGTDTLEETAYFLDLALNVNVPVAITGAMRSSNEL
HHHHHHHHHHHHHHCHHHHCCEEEECCCCHHHHHEEEEEEEEECCCCEEEEECCCCCCCC
GSDGLINLQSAILVALCPESRNKGVLVVMNDEIHNAKFVTKTHTTNVATFQTPTFGPCGL
CCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCH
IAKNRVLYFQQLTEYERFPIQTVTRTNVQLVKAYAGMDSFLLEQLAHHGCNGVVIEALGA
HHCCHHHHHHHHHHHHCCCCEEEHHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCC
GNLPPSCLAGLDALLRADIPVVLVSRAFNGVTQDVYDYLGGGKQLKQQNVIFTTGLSGQK
CCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHCCCHHHCCCCEEEEECCCCCC
ARIKLLVLLNQNLSKPLAEYF
CEEEEEEEECCCCCHHHHHHC
>Mature Secondary Structure 
TKKLLILHTGGTISMSEGEDGKVSPSEKNPLLAALERLNHPAQLSQESVLNVPSPHITL
CCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHCCHHHHCCCCCCCEEH
QHWLLLKTRIEKAVNEEQYDGIVITHGTDTLEETAYFLDLALNVNVPVAITGAMRSSNEL
HHHHHHHHHHHHHHCHHHHCCEEEECCCCHHHHHEEEEEEEEECCCCEEEEECCCCCCCC
GSDGLINLQSAILVALCPESRNKGVLVVMNDEIHNAKFVTKTHTTNVATFQTPTFGPCGL
CCCCEEECCCEEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCH
IAKNRVLYFQQLTEYERFPIQTVTRTNVQLVKAYAGMDSFLLEQLAHHGCNGVVIEALGA
HHCCHHHHHHHHHHHHCCCCEEEHHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCC
GNLPPSCLAGLDALLRADIPVVLVSRAFNGVTQDVYDYLGGGKQLKQQNVIFTTGLSGQK
CCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHCCCHHHCCCCEEEEECCCCCC
ARIKLLVLLNQNLSKPLAEYF
CEEEEEEEECCCCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]