Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 126208120

GI number: 126208120

Start: 728746

End: 730227

Strand: Direct

Name: nusA [H]

Synonym: APL_0638

Alternate gene names: 126208120

Gene position: 728746-730227 (Clockwise)

Preceding gene: 126208119

Following gene: 126208121

Centisome position: 32.04

GC content: 43.45

Gene sequence:

>1482_bases
ATGAGTAAAGAGATTTTATTAGCCGCAGAAGCGGTATCTAACGAGAAGTTATTGCCAAAAGAAGCTATCTTTGAAGCGCT
TGAAACGGCATTAGCCATTTCGACTAAAAAGAAAAAAGAGATGGATATCGATGTGCGTGTGGTAATCGACCGTAAAACCG
GTGATTTCCAAACTTTCCGCCGCTGGATTGTGGTTGAGCAAGTACACAATATGACTCGCGAGATCAGTTTAGACGCGGCG
CAATACGAAAATCCTAGCGTGCAATTAGGCGATATCGTTGAAGACGAAGTGGATTCGATTCCATTCGACCGTATTACGAT
GCAAACCGCACGCCAAGTGATCAGTACCAAAATTCGTGAAGCGGAACGTAATAAAGTGATTGAACAATTCCGTTCACAAT
TAAATACCATCATTACTGCAACCGTTAAAAAAGTAAACCGCGAGCAAATTATCTTGGATTTAGGTAATCAAGCGGAAGCG
GTAATCGTGCGTGAAGATATGCTTCCGCGTGAAAACTTCCGTCCGGGCGACCGTGTACGCGGCGTATTATATGCGATTAA
ACCTGAATCAAAAGGCCCGCAACTTTTCTTAACACGTGCAAAACCGGTCATGTTGGAAGAGCTATTTAAACTTGAAGTAC
CGGAAATCGGTGAAGATGTCATTGAAATTAAAGGCGCATCACGTGATCCGGGTTCTCGTGCGAAAATTGCGGTGAAATCA
CATGATAAACGTATCGATCCGGTCGGCGCTTGTGTCGGTATGCGCGGCGCACGTGTGCAAGCAATTAGCAACGAATTGGG
CGGCGAGCGTGTGGATATCGTACTTTGGGACGATAATCCGGCGCAATTCGTGATTAACGCAATGGCACCGGCGGAAGTAA
GCGCGATTGTCGTGGATGAAGATAAGCACGCGATGGATATTGCGGTTGAAGGTAAAAATTTAGCCCAAGCAATCGGTCGT
AACGGTCAGAACGTACGTTTAGCGACACAATTAACCGGCTGGACGTTAAATGTCATGACTACCGAAGATTTAGATAAGAA
ACATCAAGCGGAAGATAACAAAGTTATTAGTTTATTTGTTTCCGCGTTAGAAATCGACGATGAATTCGCTCAATTATTGG
TGGATGAAGGCTTCTCTAGCTTAGAAGAAATCGCATTCATTCCGGTAAGCGAATTAACCGCAATTGACGGTATCGAAGAT
GAAAATCTAGCGGAAGAATTACAAACTCGTGCGAAAAACGCAATTACCGCAAAAGCGTTAGCGGAAGAAGAAGCGTTAAA
ACAAGCGCATATTGAAGATCGTTTATTAAATTTAGAAGGTTTAGATCGCCATATTGCATTTAAACTGGCTGAAAAAGGCA
TCACAACGCTTGAAGAACTGGCGGAACAAGCAACTGACGACCTTTCGGATATTGAAGAATTATCAGCGGATAAAGCAAGC
GAATTAATTATGGCTGCACGTCAAATTTGCTGGTTTAGCTAA

Upstream 100 bases:

>100_bases
ACGCTTACGGTGGATAACGAAACTCGTCAATTCGCATTCGGTAATATTCAAAAAGCAAATTTAGTTCCGGTTTTTAATTT
TTAGAAGGTTAATCCCAAAA

Downstream 100 bases:

>100_bases
TCGTTGTGAGGAGATAAAACAATGAGTGATAACGAAATTAAAAACGAAGCACCTAAAAAATTGAGCTTACAACGTCGTAC
TAAAACTACTGTCGCGGACG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSKEILLAAEAVSNEKLLPKEAIFEALETALAISTKKKKEMDIDVRVVIDRKTGDFQTFRRWIVVEQVHNMTREISLDAA
QYENPSVQLGDIVEDEVDSIPFDRITMQTARQVISTKIREAERNKVIEQFRSQLNTIITATVKKVNREQIILDLGNQAEA
VIVREDMLPRENFRPGDRVRGVLYAIKPESKGPQLFLTRAKPVMLEELFKLEVPEIGEDVIEIKGASRDPGSRAKIAVKS
HDKRIDPVGACVGMRGARVQAISNELGGERVDIVLWDDNPAQFVINAMAPAEVSAIVVDEDKHAMDIAVEGKNLAQAIGR
NGQNVRLATQLTGWTLNVMTTEDLDKKHQAEDNKVISLFVSALEIDDEFAQLLVDEGFSSLEEIAFIPVSELTAIDGIED
ENLAEELQTRAKNAITAKALAEEEALKQAHIEDRLLNLEGLDRHIAFKLAEKGITTLEELAEQATDDLSDIEELSADKAS
ELIMAARQICWFS

Sequences:

>Translated_493_residues
MSKEILLAAEAVSNEKLLPKEAIFEALETALAISTKKKKEMDIDVRVVIDRKTGDFQTFRRWIVVEQVHNMTREISLDAA
QYENPSVQLGDIVEDEVDSIPFDRITMQTARQVISTKIREAERNKVIEQFRSQLNTIITATVKKVNREQIILDLGNQAEA
VIVREDMLPRENFRPGDRVRGVLYAIKPESKGPQLFLTRAKPVMLEELFKLEVPEIGEDVIEIKGASRDPGSRAKIAVKS
HDKRIDPVGACVGMRGARVQAISNELGGERVDIVLWDDNPAQFVINAMAPAEVSAIVVDEDKHAMDIAVEGKNLAQAIGR
NGQNVRLATQLTGWTLNVMTTEDLDKKHQAEDNKVISLFVSALEIDDEFAQLLVDEGFSSLEEIAFIPVSELTAIDGIED
ENLAEELQTRAKNAITAKALAEEEALKQAHIEDRLLNLEGLDRHIAFKLAEKGITTLEELAEQATDDLSDIEELSADKAS
ELIMAARQICWFS
>Mature_492_residues
SKEILLAAEAVSNEKLLPKEAIFEALETALAISTKKKKEMDIDVRVVIDRKTGDFQTFRRWIVVEQVHNMTREISLDAAQ
YENPSVQLGDIVEDEVDSIPFDRITMQTARQVISTKIREAERNKVIEQFRSQLNTIITATVKKVNREQIILDLGNQAEAV
IVREDMLPRENFRPGDRVRGVLYAIKPESKGPQLFLTRAKPVMLEELFKLEVPEIGEDVIEIKGASRDPGSRAKIAVKSH
DKRIDPVGACVGMRGARVQAISNELGGERVDIVLWDDNPAQFVINAMAPAEVSAIVVDEDKHAMDIAVEGKNLAQAIGRN
GQNVRLATQLTGWTLNVMTTEDLDKKHQAEDNKVISLFVSALEIDDEFAQLLVDEGFSSLEEIAFIPVSELTAIDGIEDE
NLAEELQTRAKNAITAKALAEEEALKQAHIEDRLLNLEGLDRHIAFKLAEKGITTLEELAEQATDDLSDIEELSADKASE
LIMAARQICWFS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=493, Percent_Identity=68.5598377281947, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54939; Mature: 54808

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEILLAAEAVSNEKLLPKEAIFEALETALAISTKKKKEMDIDVRVVIDRKTGDFQTFR
CCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHH
RWIVVEQVHNMTREISLDAAQYENPSVQLGDIVEDEVDSIPFDRITMQTARQVISTKIRE
HHHHHHHHHHHHHHHCCCHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AERNKVIEQFRSQLNTIITATVKKVNREQIILDLGNQAEAVIVREDMLPRENFRPGDRVR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHC
GVLYAIKPESKGPQLFLTRAKPVMLEELFKLEVPEIGEDVIEIKGASRDPGSRAKIAVKS
EEEEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCEEEEEC
HDKRIDPVGACVGMRGARVQAISNELGGERVDIVLWDDNPAQFVINAMAPAEVSAIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEEEC
DKHAMDIAVEGKNLAQAIGRNGQNVRLATQLTGWTLNVMTTEDLDKKHQAEDNKVISLFV
CCCEEEEEECCHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCHHHHHCCCCCHHHHHHH
SALEIDDEFAQLLVDEGFSSLEEIAFIPVSELTAIDGIEDENLAEELQTRAKNAITAKAL
HHHHHHHHHHHHHHHHCHHHHHHHEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AEEEALKQAHIEDRLLNLEGLDRHIAFKLAEKGITTLEELAEQATDDLSDIEELSADKAS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ELIMAARQICWFS
HHHHHHHHHHCCC
>Mature Secondary Structure 
SKEILLAAEAVSNEKLLPKEAIFEALETALAISTKKKKEMDIDVRVVIDRKTGDFQTFR
CCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHH
RWIVVEQVHNMTREISLDAAQYENPSVQLGDIVEDEVDSIPFDRITMQTARQVISTKIRE
HHHHHHHHHHHHHHHCCCHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AERNKVIEQFRSQLNTIITATVKKVNREQIILDLGNQAEAVIVREDMLPRENFRPGDRVR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCHHHC
GVLYAIKPESKGPQLFLTRAKPVMLEELFKLEVPEIGEDVIEIKGASRDPGSRAKIAVKS
EEEEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCEEEEEC
HDKRIDPVGACVGMRGARVQAISNELGGERVDIVLWDDNPAQFVINAMAPAEVSAIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEEECCCCCEEEEEEEC
DKHAMDIAVEGKNLAQAIGRNGQNVRLATQLTGWTLNVMTTEDLDKKHQAEDNKVISLFV
CCCEEEEEECCHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCHHHHHCCCCCHHHHHHH
SALEIDDEFAQLLVDEGFSSLEEIAFIPVSELTAIDGIEDENLAEELQTRAKNAITAKAL
HHHHHHHHHHHHHHHHCHHHHHHHEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AEEEALKQAHIEDRLLNLEGLDRHIAFKLAEKGITTLEELAEQATDDLSDIEELSADKAS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ELIMAARQICWFS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]