Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 126207518

GI number: 126207518

Start: 35601

End: 37181

Strand: Reverse

Name: prfC [H]

Synonym: APL_0030

Alternate gene names: 126207518

Gene position: 37181-35601 (Counterclockwise)

Preceding gene: 126207524

Following gene: 126207517

Centisome position: 1.63

GC content: 44.15

Gene sequence:

>1581_bases
ATGTCATATCCACAAGAAGTCAATAAACGTCGTACCTTTGCGATTATTTCTCACCCCGATGCGGGTAAAACCACCATAAC
CGAAAAAGTTTTACTTTACGGAAATGCAATCCAAACCGCCGGTTCAGTGAAAGGAAAAGGCTCTAGCGCACACGCCAAAT
CCGACTGGATGGAAATGGAAAAACAACGCGGTATTTCTATTACAACGTCCGTCATGCAATTTCCTTACAATAATTGTTTA
GTTAATTTATTGGATACCCCCGGACACGAAGACTTCTCCGAAGATACTTACCGCACATTGACTGCGGTAGATAGCTGCTT
AATGGTTATCGACAGTGCAAAAGGTGTTGAGGAACGTACAATTAAATTAATAGAAGTGACTCGTCTGCGTGATACGCCGA
TCTTAACTTTTATGAACAAACTCGACCGTGATATCCGTGATCCGATGGAGTTACTAGATGAAGTTGAAAGCGTCCTAAAA
ATCCGTTGTGCGCCGATTACTTGGCCGATTGGTTGCGGTAAATTATTTAAAGGCGTCTATCATATTGCAAAAGACGAAAC
CTATCTTTATCAATCCGGTCAAGGTTCAACCATTCAAGAAGTACGTATCGTAAAAGGCTTGAGCAGCCCGGAACTTGATG
CTGCGGTCGGTGATGATTTAGCTAACCAGTTACGTGAAGAATTAGAACTTGTACAAGGTGCATCAAACGAATTTGATCAC
GAAGCCTTTATCAACGGCGAACTAACACCGGTATTCTTCGGTACCGCATTAGGTAACTTCGGTGTGGATCATTTCCTAGA
CGGTTTAACCGAATGGGCGCCAAAACCGCAAGCACGTCAAACCGATGTACGTACGGTCGAATCAAGCGAGGAAAAATTCT
CTGGCTTCGTGTTTAAGATCCAAGCGAATATGGACCCGAAACACCGTGACCGAGTGGCATTTATGCGCGTTGTTTCAGGC
AAATACGAAAAAGGTATGAAGCTCAAACACGTACGTATCGGCAAAGATGTCGTGATTTCCGATGCCTTAACCTTTATGGC
GGGTGATCGTGCACATGCTGAAGAAGCTTACGCCGGCGATATTATCGGTTTACATAACCACGGCACAATCCAAATCGGCG
ACACCTTCACTCAAGGTGAAGTGATGAAATTTACCGGTATTCCGAACTTTGCACCGGAACTGTTCCGTCGTATTCGCTTG
AAAGACCCACTTAAGCAAAAACAATTACTGAAAGGCTTAGTTCAACTTTCGGAAGAAGGTGCTGTACAGGTGTTCCGTCC
GTTAATGAACAATGACTTGATTGTAGGTGCGGTCGGTGTGCTTCAGTTTGACGTGGTGGTTTCCCGTCTGAAAACCGAAT
ATAACGTGGAAGCGATTTACGAAGCGGTAAACGTTGCAACCGCTCGTTGGGTTGAGTGCGGTGATGCGAAGAAATTTGAA
GAATTTAAACGCAAAAACGAACAAAACCTCGCATTAGACGGCGGCGATAACTTAACTTATATCGCACCGACTATGGTGAA
CTTAAATCTGGCGCAAGAGCGTTATCCCGACATAAACTTCTTTAAAACACGCGAACATTAA

Upstream 100 bases:

>100_bases
GCTTAGGTTGTCCTCAAAGCATAAATCAGCGAAAATAGATCAATATTTTTAAGGTGGTTCGTCCGCCCTTCTTTGTCTTA
ATTTTAATCTAGAGAAAAGC

Downstream 100 bases:

>100_bases
TCTTTGCTAAACAAGCGGTCATTTTGTAGAGAAATTCGACAAAATGACCGCTTTTTTATCCTTCTAATTCTCCTCTTTTC
TTATTCCATTATTTTTTTAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSSAHAKSDWMEMEKQRGISITTSVMQFPYNNCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLIEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
IRCAPITWPIGCGKLFKGVYHIAKDETYLYQSGQGSTIQEVRIVKGLSSPELDAAVGDDLANQLREELELVQGASNEFDH
EAFINGELTPVFFGTALGNFGVDHFLDGLTEWAPKPQARQTDVRTVESSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSG
KYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGEVMKFTGIPNFAPELFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYEAVNVATARWVECGDAKKFE
EFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDINFFKTREH

Sequences:

>Translated_526_residues
MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSSAHAKSDWMEMEKQRGISITTSVMQFPYNNCL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLIEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
IRCAPITWPIGCGKLFKGVYHIAKDETYLYQSGQGSTIQEVRIVKGLSSPELDAAVGDDLANQLREELELVQGASNEFDH
EAFINGELTPVFFGTALGNFGVDHFLDGLTEWAPKPQARQTDVRTVESSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSG
KYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGEVMKFTGIPNFAPELFRRIRL
KDPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYEAVNVATARWVECGDAKKFE
EFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDINFFKTREH
>Mature_525_residues
SYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSSAHAKSDWMEMEKQRGISITTSVMQFPYNNCLV
NLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLIEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLKI
RCAPITWPIGCGKLFKGVYHIAKDETYLYQSGQGSTIQEVRIVKGLSSPELDAAVGDDLANQLREELELVQGASNEFDHE
AFINGELTPVFFGTALGNFGVDHFLDGLTEWAPKPQARQTDVRTVESSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGK
YEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGDIIGLHNHGTIQIGDTFTQGEVMKFTGIPNFAPELFRRIRLK
DPLKQKQLLKGLVQLSEEGAVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYEAVNVATARWVECGDAKKFEE
FKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDINFFKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=28.1124497991968, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI19923640, Length=516, Percent_Identity=25.1937984496124, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306283, Length=400, Percent_Identity=27.25, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI25306287, Length=488, Percent_Identity=25.4098360655738, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=28.4810126582279, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=86.8320610687023, Blast_Score=961, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=504, Percent_Identity=25.7936507936508, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788922, Length=168, Percent_Identity=30.952380952381, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=28.3720930232558, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=515, Percent_Identity=27.1844660194175, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=163, Percent_Identity=32.5153374233129, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=29.6296296296296, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=27.2916666666667, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=416, Percent_Identity=25.9615384615385, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.1655629139073, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=26.6233766233766, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=26.6233766233766, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=478, Percent_Identity=26.7782426778243, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=23.8003838771593, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI78706572, Length=237, Percent_Identity=25.3164556962025, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58981; Mature: 58850

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSSAHAKSDWMEME
CCCCHHHCCCEEEEEEECCCCCCCHHHHHHHEECCHHHCCCCCCCCCCCCCCHHHHHHHH
KQRGISITTSVMQFPYNNCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERT
HHCCCEEEHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
IKLIEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLKIRCAPITWPIGCGKLFKGVY
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
HIAKDETYLYQSGQGSTIQEVRIVKGLSSPELDAAVGDDLANQLREELELVQGASNEFDH
HHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EAFINGELTPVFFGTALGNFGVDHFLDGLTEWAPKPQARQTDVRTVESSEEKFSGFVFKI
CEEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHCCEEEEE
QANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGD
ECCCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCC
IIGLHNHGTIQIGDTFTQGEVMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYEAVNVATARWVECGDAKKFE
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHH
EFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDINFFKTREH
HHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCCCCEECCCC
>Mature Secondary Structure 
SYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVKGKGSSAHAKSDWMEME
CCCHHHCCCEEEEEEECCCCCCCHHHHHHHEECCHHHCCCCCCCCCCCCCCHHHHHHHH
KQRGISITTSVMQFPYNNCLVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERT
HHCCCEEEHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
IKLIEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLKIRCAPITWPIGCGKLFKGVY
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
HIAKDETYLYQSGQGSTIQEVRIVKGLSSPELDAAVGDDLANQLREELELVQGASNEFDH
HHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EAFINGELTPVFFGTALGNFGVDHFLDGLTEWAPKPQARQTDVRTVESSEEKFSGFVFKI
CEEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHCCEEEEE
QANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIGKDVVISDALTFMAGDRAHAEEAYAGD
ECCCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCC
IIGLHNHGTIQIGDTFTQGEVMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AVQVFRPLMNNDLIVGAVGVLQFDVVVSRLKTEYNVEAIYEAVNVATARWVECGDAKKFE
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHH
EFKRKNEQNLALDGGDNLTYIAPTMVNLNLAQERYPDINFFKTREH
HHHHCCCCCEEEECCCCEEEEECEEEEEEEHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA