Click here to switch to the map view.

The map label for this gene is nikA [H]

Identifier: 126207517

GI number: 126207517

Start: 33833

End: 35407

Strand: Reverse

Name: nikA [H]

Synonym: APL_0029

Alternate gene names: 126207517

Gene position: 35407-33833 (Counterclockwise)

Preceding gene: 126207518

Following gene: 126207516

Centisome position: 1.56

GC content: 39.94

Gene sequence:

>1575_bases
ATGAAAAAATGGCTACTTCCGTTACTGGCAACCACATTATTTACCGTAGGTTGCGAAGATAAATCCGTTACACAAAACAA
TCCGCAAAATAATAAAGAAAGCGAAGTTAAAAACTTAAAAAACAATGAATTAACTCAAGTCACCGTAATTGCGCCTTGGG
AGTTAAACAGTTTGCATCCGACTCAATCCGGCGTTATTTTCCAACGAATGAACTTAGCCGAAACTTTAGTTGAAGCGAAT
GAAAAAGGCGAATTGATTCCGGGATTGGCAACAGAATGGCAAAGTAATGAAGACGCAACCGAGTGGACTTTTACTCTACG
TGATGCAACCTTCCATAACGGTGAAAAAGTAAATGCGCAAACCGTAGCCAAAAATTTCAATTTATTAATGGCGCAACCGA
GCATTCTACAAAAAGCCTTTATTCAAGAGATCAGTGCATTAGGGGACAATCAAGTTAAATTTAAACTCACGAAATCGTTC
GTACCCTTCCCATCTTTTTTAACGCACTATTCCACCATTATTTTAGCGGACGAAGCATTTAATGAAAAAAACGAAGTGGT
GAAATTAATCGGTACCGGTGCTTTTAAAGCGACCAAAGTCGAAGCACCGCAAAAAATTGAAGCGGTTCGTTTTGAGCAAT
ACTGGGGAGAAAAACCGAAGGTTCTTCATGCAAATTATTTAGCCAGCAGCCGTAGTGAAACTCGTGTATTAATGGCACAA
AGCGATGTAAGTTCATTAGTATTTAATTTAGATACCGCCAGTGTCGCACGTTTAAAAACCGATCCGAATTTAACTGTTAC
CAGCGGTTCTATCGCTCGTACGATTCAAATGAAGATGGATGCGGCTAAACCGTTCTTTAAAGATTTAACAATTCGCCAAG
CACTTTCACAGGCAATTAACCGTAAGGCGATTGCCGAACAAGTGTTAAAAATTAAAGACGGTGCGGCGGATCAAATCTTA
CCGAAAGCCCTTGCCGATTGGCGTGTGGAAAATAAAGACGAAAGCGTTGATATTGCAAAAATCAAACAAAATTTAACCTC
TGCCGGATACCAATTCAATAGCGAAGGCAAATTAACCGATAAAGAAGGAAAGCCGTTCAGCTTTACCTTAAGAACCTTCT
CGGATCGTCCGGAATTACCGATTATTGCGACTATTCTCCAAGCACAATGGCGAGAGATTGGTATTGATGTCAATGTTTCT
GTCGGCAATTCGAGTGAAATTCCTGCCGGTCATAAAGACGGTAGCTTAGAAATGGCACTCTATGCGCTGAACTATGGCAA
AACCATCGATCCGTTCGGTGTTATCGTACAAGATTATGCAAAAGGCGGTAGCGACTGGGGTGTAATGAACTGGAGCAATG
AACAATTAGCACAAACGTTAGAGAAAATCGAAACGGAACGTGATCCGCAAAAAGCTAAGCAGTTAAAACAAACGGTTAGC
CGAATCATTCATGATGAATTACCGATTATTCCAATTGTGTATTATCAGCAAAATGTCGCAACACATAATGAGATGAAAGG
CGTTACGCTAGATACATTCGAAAGAAGATTCTTTTTAGAAAAACTCACAAAATAA

Upstream 100 bases:

>100_bases
TTTTTATTGATTTAATAGATTAATAGCTATTATTTTTTTATGGTTTTATGCTAGAATTGCCGCGCTATTAAATACTTGAC
TAAAAATAAGGGAATTTCTA

Downstream 100 bases:

>100_bases
CATGATAAAAATTTAGCATTAAAAACACGGAAAACAGCATACAAATTTAAGCCGCTTTCCGTGTTTTTTATATGAATTCT
CACGGTTAAAAATATGCTTA

Product: ABC transporter periplasmic protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKKWLLPLLATTLFTVGCEDKSVTQNNPQNNKESEVKNLKNNELTQVTVIAPWELNSLHPTQSGVIFQRMNLAETLVEAN
EKGELIPGLATEWQSNEDATEWTFTLRDATFHNGEKVNAQTVAKNFNLLMAQPSILQKAFIQEISALGDNQVKFKLTKSF
VPFPSFLTHYSTIILADEAFNEKNEVVKLIGTGAFKATKVEAPQKIEAVRFEQYWGEKPKVLHANYLASSRSETRVLMAQ
SDVSSLVFNLDTASVARLKTDPNLTVTSGSIARTIQMKMDAAKPFFKDLTIRQALSQAINRKAIAEQVLKIKDGAADQIL
PKALADWRVENKDESVDIAKIKQNLTSAGYQFNSEGKLTDKEGKPFSFTLRTFSDRPELPIIATILQAQWREIGIDVNVS
VGNSSEIPAGHKDGSLEMALYALNYGKTIDPFGVIVQDYAKGGSDWGVMNWSNEQLAQTLEKIETERDPQKAKQLKQTVS
RIIHDELPIIPIVYYQQNVATHNEMKGVTLDTFERRFFLEKLTK

Sequences:

>Translated_524_residues
MKKWLLPLLATTLFTVGCEDKSVTQNNPQNNKESEVKNLKNNELTQVTVIAPWELNSLHPTQSGVIFQRMNLAETLVEAN
EKGELIPGLATEWQSNEDATEWTFTLRDATFHNGEKVNAQTVAKNFNLLMAQPSILQKAFIQEISALGDNQVKFKLTKSF
VPFPSFLTHYSTIILADEAFNEKNEVVKLIGTGAFKATKVEAPQKIEAVRFEQYWGEKPKVLHANYLASSRSETRVLMAQ
SDVSSLVFNLDTASVARLKTDPNLTVTSGSIARTIQMKMDAAKPFFKDLTIRQALSQAINRKAIAEQVLKIKDGAADQIL
PKALADWRVENKDESVDIAKIKQNLTSAGYQFNSEGKLTDKEGKPFSFTLRTFSDRPELPIIATILQAQWREIGIDVNVS
VGNSSEIPAGHKDGSLEMALYALNYGKTIDPFGVIVQDYAKGGSDWGVMNWSNEQLAQTLEKIETERDPQKAKQLKQTVS
RIIHDELPIIPIVYYQQNVATHNEMKGVTLDTFERRFFLEKLTK
>Mature_524_residues
MKKWLLPLLATTLFTVGCEDKSVTQNNPQNNKESEVKNLKNNELTQVTVIAPWELNSLHPTQSGVIFQRMNLAETLVEAN
EKGELIPGLATEWQSNEDATEWTFTLRDATFHNGEKVNAQTVAKNFNLLMAQPSILQKAFIQEISALGDNQVKFKLTKSF
VPFPSFLTHYSTIILADEAFNEKNEVVKLIGTGAFKATKVEAPQKIEAVRFEQYWGEKPKVLHANYLASSRSETRVLMAQ
SDVSSLVFNLDTASVARLKTDPNLTVTSGSIARTIQMKMDAAKPFFKDLTIRQALSQAINRKAIAEQVLKIKDGAADQIL
PKALADWRVENKDESVDIAKIKQNLTSAGYQFNSEGKLTDKEGKPFSFTLRTFSDRPELPIIATILQAQWREIGIDVNVS
VGNSSEIPAGHKDGSLEMALYALNYGKTIDPFGVIVQDYAKGGSDWGVMNWSNEQLAQTLEKIETERDPQKAKQLKQTVS
RIIHDELPIIPIVYYQQNVATHNEMKGVTLDTFERRFFLEKLTK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=480, Percent_Identity=25, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=23.1914893617021, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787052, Length=435, Percent_Identity=21.6091954022989, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081878, Length=483, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787551, Length=502, Percent_Identity=22.3107569721116, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58757; Mature: 58757

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLPLLATTLFTVGCEDKSVTQNNPQNNKESEVKNLKNNELTQVTVIAPWELNSLHP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCC
TQSGVIFQRMNLAETLVEANEKGELIPGLATEWQSNEDATEWTFTLRDATFHNGEKVNAQ
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCHH
TVAKNFNLLMAQPSILQKAFIQEISALGDNQVKFKLTKSFVPFPSFLTHYSTIILADEAF
HHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEEEEECHHC
NEKNEVVKLIGTGAFKATKVEAPQKIEAVRFEQYWGEKPKVLHANYLASSRSETRVLMAQ
CCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCEEEEEC
SDVSSLVFNLDTASVARLKTDPNLTVTSGSIARTIQMKMDAAKPFFKDLTIRQALSQAIN
CCHHHHEEECCCHHEEEEECCCCEEEECCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHH
RKAIAEQVLKIKDGAADQILPKALADWRVENKDESVDIAKIKQNLTSAGYQFNSEGKLTD
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCEEC
KEGKPFSFTLRTFSDRPELPIIATILQAQWREIGIDVNVSVGNSSEIPAGHKDGSLEMAL
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEE
YALNYGKTIDPFGVIVQDYAKGGSDWGVMNWSNEQLAQTLEKIETERDPQKAKQLKQTVS
EEECCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RIIHDELPIIPIVYYQQNVATHNEMKGVTLDTFERRFFLEKLTK
HHHHCCCCCEEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKWLLPLLATTLFTVGCEDKSVTQNNPQNNKESEVKNLKNNELTQVTVIAPWELNSLHP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCC
TQSGVIFQRMNLAETLVEANEKGELIPGLATEWQSNEDATEWTFTLRDATFHNGEKVNAQ
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCCHH
TVAKNFNLLMAQPSILQKAFIQEISALGDNQVKFKLTKSFVPFPSFLTHYSTIILADEAF
HHHCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEEEEECHHC
NEKNEVVKLIGTGAFKATKVEAPQKIEAVRFEQYWGEKPKVLHANYLASSRSETRVLMAQ
CCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCEEEEEC
SDVSSLVFNLDTASVARLKTDPNLTVTSGSIARTIQMKMDAAKPFFKDLTIRQALSQAIN
CCHHHHEEECCCHHEEEEECCCCEEEECCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHH
RKAIAEQVLKIKDGAADQILPKALADWRVENKDESVDIAKIKQNLTSAGYQFNSEGKLTD
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCEEC
KEGKPFSFTLRTFSDRPELPIIATILQAQWREIGIDVNVSVGNSSEIPAGHKDGSLEMAL
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEE
YALNYGKTIDPFGVIVQDYAKGGSDWGVMNWSNEQLAQTLEKIETERDPQKAKQLKQTVS
EEECCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RIIHDELPIIPIVYYQQNVATHNEMKGVTLDTFERRFFLEKLTK
HHHHCCCCCEEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]