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The map label for this gene is tnsA [H]

Identifier: 126176541

GI number: 126176541

Start: 5102646

End: 5103467

Strand: Reverse

Name: tnsA [H]

Synonym: Sbal_4360

Alternate gene names: 126176541

Gene position: 5103467-5102646 (Counterclockwise)

Preceding gene: 126176542

Following gene: 126176540

Centisome position: 99.53

GC content: 39.05

Gene sequence:

>822_bases
ATGGGTAAGAAACAGTACTGGAACTCTGAGGTTAAAAATCGACGTTGGATTAAAGAAGGTCGAGGTCAAGGCACCGGAGC
AGACTATAAACCATGGCTTACCGTTCGAGATGTAGCTTCAGAAGGACGGTCTCATCGTGTTTTTGGACACATTTCTCAAC
GAACCCATCATCTACTATCTGATCTAGAGCTTGCAACATTTCTTCTGCTTCAATGGCGCTCAACTACTATAGATATTCGA
GAACAATTCCCCCTCGATCGTGCAGTGACGCTGGATATCAGTAAACGATTAGGAATAGAACATCCAAACCATCATGGGGT
GGAGCAATATATGAGTAGTGATTTTGTTGTAGATACTGAAGAAAAAGATAGGCGATTTGCCATTCAGGTTAAAGCGACTG
AAGCCTTTAGTGACTCTAGAACAATTGAAAAGCTTGAGATTGAAAGTCAATATTGGCGTGAGAAAGGAGTACCTTTTTTT
TGGGTCACTGAAAATCAGATTCCTCCAATTGTATTTGAGAATATTAACCTTCTTTATAATCATATTGACTATGACACCGG
TCAGAATGATCTCTTAGTTCAATTAGACGTTTTTAGTAAACATATACGACAGAGTGAAAACCTGAGGGTTAAAGAGTTAT
GTATGAAACTCGACGCTTCCTATGATCATGAACCTGGTGAGGCTTTATATCAAATTAAACGATTGCTTGCACATCGCTAT
TTTCATTTTGATATAACTAAACCATTTACAGAGCTAAGGTGTTCTGACATAACAGCTGAAACTTTCAATGCTTTGCAGGA
GGGTTGGCGTGTTTCAAGTTAA

Upstream 100 bases:

>100_bases
TTGTTACCTACACAATATAGTTGTTAACTTTACAATTTAGTTGTTAATTATTGCCATCTGAGCTACTTTTAAAATAAGTC
AGTTTAGATGGCATTTTACT

Downstream 100 bases:

>100_bases
CGAGGTTATCAAGCTAGAAGACAAGCTTTATAGAGTGCTTGTAACTACAAATACGCATGCTGTATGGATTTCCCTTGAAG
ATCAAAAGGCATTCCCAGAT

Product: transposon Tn7 transposition protein TnsA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGKKQYWNSEVKNRRWIKEGRGQGTGADYKPWLTVRDVASEGRSHRVFGHISQRTHHLLSDLELATFLLLQWRSTTIDIR
EQFPLDRAVTLDISKRLGIEHPNHHGVEQYMSSDFVVDTEEKDRRFAIQVKATEAFSDSRTIEKLEIESQYWREKGVPFF
WVTENQIPPIVFENINLLYNHIDYDTGQNDLLVQLDVFSKHIRQSENLRVKELCMKLDASYDHEPGEALYQIKRLLAHRY
FHFDITKPFTELRCSDITAETFNALQEGWRVSS

Sequences:

>Translated_273_residues
MGKKQYWNSEVKNRRWIKEGRGQGTGADYKPWLTVRDVASEGRSHRVFGHISQRTHHLLSDLELATFLLLQWRSTTIDIR
EQFPLDRAVTLDISKRLGIEHPNHHGVEQYMSSDFVVDTEEKDRRFAIQVKATEAFSDSRTIEKLEIESQYWREKGVPFF
WVTENQIPPIVFENINLLYNHIDYDTGQNDLLVQLDVFSKHIRQSENLRVKELCMKLDASYDHEPGEALYQIKRLLAHRY
FHFDITKPFTELRCSDITAETFNALQEGWRVSS
>Mature_272_residues
GKKQYWNSEVKNRRWIKEGRGQGTGADYKPWLTVRDVASEGRSHRVFGHISQRTHHLLSDLELATFLLLQWRSTTIDIRE
QFPLDRAVTLDISKRLGIEHPNHHGVEQYMSSDFVVDTEEKDRRFAIQVKATEAFSDSRTIEKLEIESQYWREKGVPFFW
VTENQIPPIVFENINLLYNHIDYDTGQNDLLVQLDVFSKHIRQSENLRVKELCMKLDASYDHEPGEALYQIKRLLAHRYF
HFDITKPFTELRCSDITAETFNALQEGWRVSS

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 32171; Mature: 32039

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKQYWNSEVKNRRWIKEGRGQGTGADYKPWLTVRDVASEGRSHRVFGHISQRTHHLLS
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHCCCCCEEEEHHHHHHHHHHH
DLELATFLLLQWRSTTIDIREQFPLDRAVTLDISKRLGIEHPNHHGVEQYMSSDFVVDTE
HHHHHHHHHHHHCCCEEEHHHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHHCCCCEEECC
EKDRRFAIQVKATEAFSDSRTIEKLEIESQYWREKGVPFFWVTENQIPPIVFENINLLYN
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHHHHE
HIDYDTGQNDLLVQLDVFSKHIRQSENLRVKELCMKLDASYDHEPGEALYQIKRLLAHRY
ECCCCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FHFDITKPFTELRCSDITAETFNALQEGWRVSS
EEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKKQYWNSEVKNRRWIKEGRGQGTGADYKPWLTVRDVASEGRSHRVFGHISQRTHHLLS
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHCCCCCEEEEHHHHHHHHHHH
DLELATFLLLQWRSTTIDIREQFPLDRAVTLDISKRLGIEHPNHHGVEQYMSSDFVVDTE
HHHHHHHHHHHHCCCEEEHHHHCCCCCEEEEEEHHHCCCCCCCCCCHHHHHCCCCEEECC
EKDRRFAIQVKATEAFSDSRTIEKLEIESQYWREKGVPFFWVTENQIPPIVFENINLLYN
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHHHHE
HIDYDTGQNDLLVQLDVFSKHIRQSENLRVKELCMKLDASYDHEPGEALYQIKRLLAHRY
ECCCCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FHFDITKPFTELRCSDITAETFNALQEGWRVSS
EEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]