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The map label for this gene is tnsB [H]

Identifier: 126176540

GI number: 126176540

Start: 5100587

End: 5102659

Strand: Reverse

Name: tnsB [H]

Synonym: Sbal_4359

Alternate gene names: 126176540

Gene position: 5102659-5100587 (Counterclockwise)

Preceding gene: 126176541

Following gene: 126176539

Centisome position: 99.52

GC content: 38.83

Gene sequence:

>2073_bases
GTGTTTCAAGTTAACGAGGTTATCAAGCTAGAAGACAAGCTTTATAGAGTGCTTGTAACTACAAATACGCATGCTGTATG
GATTTCCCTTGAAGATCAAAAGGCATTCCCAGATCTTGTTGAGCTTTCACAACTCGAAGATCTTTTATTAGAAGAAAAGC
TAACTCGCGTTGACGATCCATTTGAATATATAGTAAACCTCTTGCCTGAACCTGACTCCAAAGAAACAACAATTCGAAAT
AGAAATTATCAAATCATAAAACCGCTTGTAGAAAATCCTAATTTTTATATAAAAAAAGTTCGAGCAAAACATGTAGCGAC
TATTCTAAAATCTGGTGAAATATCAAGGCCATACCTTTATAAGTTGATTCGTCGTTATTGGCAAAGAGGACAGGTTTCCA
ATACCTTATTGCCTGATTACAGGAATTCTGGAGCGAAAGGTCAGAAACGTGTTGCTAATGACAAAAAGCTCGGCCGTCCA
AGAAAAATTATGGAGGGAACTGGTGCCTTGGTAGATGAGCCAACTGAAAAACTATTTCGAATAATTATTGATAAATATAT
TTTCAATAAACAATTCTCTATTGCTCGTGCACATCGTAAATTCAAAGGTCTTTATGACAACATATTTCCTGGTGTACCTG
AATCAGAAAAACCAACAAAACGCCAATTAAGCTACTTCTTAGATCGTGAATACAAGAGTGTAGATAAAATCAAGGCTGGT
GTATCGGATATTATTTATAAAAAAGATGTCCGCCCTTTGCATAGTACTGCAACAATTCAAGCTTTAGGGCCTGGTTCTCG
TTATGAGATAGATGCCACCATTGCAGACGTAATATTAGTATCAGATCATGATAGAAATCAGCCTGTCGGACGACCTACTG
TATATATTGTAATTGATGTTTTTTCTAGGCTAATTGTTGGCTGGTACATCGGTTTTGAAAACCCGTCTTACGTAGCTGCA
ATACAATCACTTTATTTGGCATTAAATGACAAAAAGCATTTATTCAAAGATTTAGATATTGAAACTGATAATTTTTCTTG
GCCTACCCCTGGTTTACCAGAAGCCATACTTGCTGATAGAGGCGAACTTATCGGTCATCAAATTGAAGGTTTAGAATCAA
GTTACAGAGTTCGAATCGAAAATACGCCCCCGTATAGAGGAGATGCAAAAGGAATTGTCGAACAAAGGTTTAGAACGCTA
CAGGCAGAATTCAAAAAATTTACGCCTGGGGAAGTAATTGGCCCTACAGTAAGAAAGAGGGGCGGTAAGAACTATTGGCT
TGATGGGAAGTTAACTATTTCAGAGTTTACCGAAATCATAGTTTCATCTATTGTGATGAGAAATTTTGTAGATCCTATGT
CAAAGTATGATCGAGCAAAAGATATGCCTGCGAATTTACCTTCAATACCTATTCATCTTTGGAACTGGGGCCTTCAAAAT
AGGACCGGACGGTTACGTAAAGCTAATGCGGAATCGGTGCGTATTGCATTATTGCCCAGAAATCAAGCTAGTGTGTCAGC
GCAAGGTATATGTATATTTGGGGTTTATTACTCCGGACCTGAAATTGTTGAACTTGGCTGGATGCATCGTTCGGACAAAT
CAACCAGGCCTAAAAAAGTTGAGGTAGCTTTCGACCCAAACTTTGCCGATGAGATTTATTTATTTCATACAGCAGGAAGT
CGAGAGCACTGGGTATGTCGCATTACTGATTTATCGCGGGAGTTTAGAGGGCAAACATTTTGGGAAGTTTGGCGAAAACA
GAAACAGATCAAGATGCAGGGATCGAAAGATCAGCTACACGCTGACAAACTTCGAAAGCATCATGAAAATCGTGTAGAAG
ACATCATTTCGTCGGCAATTAAGCAAACACCGAAACAGACTGCTACAAATGTAGATCGTATGAAAGGAGTTAGTTATGCT
CGGTCTGATCAGCTTGCTAAAGAACGGGAGTTGCGCCGACCTAAAAGACCTGAACGCCAACAAACAGCAAAAGTAGTTCA
TCTAACTCCGCCTGAAGATAGTGATGAATACCCTGATTTTGAAGATGAATTGTTTGATGGTGGAGACTCTTGA

Upstream 100 bases:

>100_bases
GCACATCGCTATTTTCATTTTGATATAACTAAACCATTTACAGAGCTAAGGTGTTCTGACATAACAGCTGAAACTTTCAA
TGCTTTGCAGGAGGGTTGGC

Downstream 100 bases:

>100_bases
TGGAACAACCCAATCACTTTGTTATAGCGTCTTACCAGACTACGGGCATTAAGCGTTACGATGGTAATCCATTTATTGAA
GCGCTTCCTCCTATATTGTC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MFQVNEVIKLEDKLYRVLVTTNTHAVWISLEDQKAFPDLVELSQLEDLLLEEKLTRVDDPFEYIVNLLPEPDSKETTIRN
RNYQIIKPLVENPNFYIKKVRAKHVATILKSGEISRPYLYKLIRRYWQRGQVSNTLLPDYRNSGAKGQKRVANDKKLGRP
RKIMEGTGALVDEPTEKLFRIIIDKYIFNKQFSIARAHRKFKGLYDNIFPGVPESEKPTKRQLSYFLDREYKSVDKIKAG
VSDIIYKKDVRPLHSTATIQALGPGSRYEIDATIADVILVSDHDRNQPVGRPTVYIVIDVFSRLIVGWYIGFENPSYVAA
IQSLYLALNDKKHLFKDLDIETDNFSWPTPGLPEAILADRGELIGHQIEGLESSYRVRIENTPPYRGDAKGIVEQRFRTL
QAEFKKFTPGEVIGPTVRKRGGKNYWLDGKLTISEFTEIIVSSIVMRNFVDPMSKYDRAKDMPANLPSIPIHLWNWGLQN
RTGRLRKANAESVRIALLPRNQASVSAQGICIFGVYYSGPEIVELGWMHRSDKSTRPKKVEVAFDPNFADEIYLFHTAGS
REHWVCRITDLSREFRGQTFWEVWRKQKQIKMQGSKDQLHADKLRKHHENRVEDIISSAIKQTPKQTATNVDRMKGVSYA
RSDQLAKERELRRPKRPERQQTAKVVHLTPPEDSDEYPDFEDELFDGGDS

Sequences:

>Translated_690_residues
MFQVNEVIKLEDKLYRVLVTTNTHAVWISLEDQKAFPDLVELSQLEDLLLEEKLTRVDDPFEYIVNLLPEPDSKETTIRN
RNYQIIKPLVENPNFYIKKVRAKHVATILKSGEISRPYLYKLIRRYWQRGQVSNTLLPDYRNSGAKGQKRVANDKKLGRP
RKIMEGTGALVDEPTEKLFRIIIDKYIFNKQFSIARAHRKFKGLYDNIFPGVPESEKPTKRQLSYFLDREYKSVDKIKAG
VSDIIYKKDVRPLHSTATIQALGPGSRYEIDATIADVILVSDHDRNQPVGRPTVYIVIDVFSRLIVGWYIGFENPSYVAA
IQSLYLALNDKKHLFKDLDIETDNFSWPTPGLPEAILADRGELIGHQIEGLESSYRVRIENTPPYRGDAKGIVEQRFRTL
QAEFKKFTPGEVIGPTVRKRGGKNYWLDGKLTISEFTEIIVSSIVMRNFVDPMSKYDRAKDMPANLPSIPIHLWNWGLQN
RTGRLRKANAESVRIALLPRNQASVSAQGICIFGVYYSGPEIVELGWMHRSDKSTRPKKVEVAFDPNFADEIYLFHTAGS
REHWVCRITDLSREFRGQTFWEVWRKQKQIKMQGSKDQLHADKLRKHHENRVEDIISSAIKQTPKQTATNVDRMKGVSYA
RSDQLAKERELRRPKRPERQQTAKVVHLTPPEDSDEYPDFEDELFDGGDS
>Mature_690_residues
MFQVNEVIKLEDKLYRVLVTTNTHAVWISLEDQKAFPDLVELSQLEDLLLEEKLTRVDDPFEYIVNLLPEPDSKETTIRN
RNYQIIKPLVENPNFYIKKVRAKHVATILKSGEISRPYLYKLIRRYWQRGQVSNTLLPDYRNSGAKGQKRVANDKKLGRP
RKIMEGTGALVDEPTEKLFRIIIDKYIFNKQFSIARAHRKFKGLYDNIFPGVPESEKPTKRQLSYFLDREYKSVDKIKAG
VSDIIYKKDVRPLHSTATIQALGPGSRYEIDATIADVILVSDHDRNQPVGRPTVYIVIDVFSRLIVGWYIGFENPSYVAA
IQSLYLALNDKKHLFKDLDIETDNFSWPTPGLPEAILADRGELIGHQIEGLESSYRVRIENTPPYRGDAKGIVEQRFRTL
QAEFKKFTPGEVIGPTVRKRGGKNYWLDGKLTISEFTEIIVSSIVMRNFVDPMSKYDRAKDMPANLPSIPIHLWNWGLQN
RTGRLRKANAESVRIALLPRNQASVSAQGICIFGVYYSGPEIVELGWMHRSDKSTRPKKVEVAFDPNFADEIYLFHTAGS
REHWVCRITDLSREFRGQTFWEVWRKQKQIKMQGSKDQLHADKLRKHHENRVEDIISSAIKQTPKQTATNVDRMKGVSYA
RSDQLAKERELRRPKRPERQQTAKVVHLTPPEDSDEYPDFEDELFDGGDS

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 79677; Mature: 79677

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQVNEVIKLEDKLYRVLVTTNTHAVWISLEDQKAFPDLVELSQLEDLLLEEKLTRVDDP
CCCCHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
FEYIVNLLPEPDSKETTIRNRNYQIIKPLVENPNFYIKKVRAKHVATILKSGEISRPYLY
HHHHHHHCCCCCCCCCEECCCCCEEEHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCHHHH
KLIRRYWQRGQVSNTLLPDYRNSGAKGQKRVANDKKLGRPRKIMEGTGALVDEPTEKLFR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCHHHHHCCCCCCCCHHHHHHH
IIIDKYIFNKQFSIARAHRKFKGLYDNIFPGVPESEKPTKRQLSYFLDREYKSVDKIKAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSDIIYKKDVRPLHSTATIQALGPGSRYEIDATIADVILVSDHDRNQPVGRPTVYIVIDV
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEHHHH
FSRLIVGWYIGFENPSYVAAIQSLYLALNDKKHLFKDLDIETDNFSWPTPGLPEAILADR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCC
GELIGHQIEGLESSYRVRIENTPPYRGDAKGIVEQRFRTLQAEFKKFTPGEVIGPTVRKR
HHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
GGKNYWLDGKLTISEFTEIIVSSIVMRNFVDPMSKYDRAKDMPANLPSIPIHLWNWGLQN
CCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCC
RTGRLRKANAESVRIALLPRNQASVSAQGICIFGVYYSGPEIVELGWMHRSDKSTRPKKV
CCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEECCEECCCCCCCCCEE
EVAFDPNFADEIYLFHTAGSREHWVCRITDLSREFRGQTFWEVWRKQKQIKMQGSKDQLH
EEEECCCCCCEEEEEEECCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHH
ADKLRKHHENRVEDIISSAIKQTPKQTATNVDRMKGVSYARSDQLAKERELRRPKRPERQ
HHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCH
QTAKVVHLTPPEDSDEYPDFEDELFDGGDS
HCCEEEEECCCCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MFQVNEVIKLEDKLYRVLVTTNTHAVWISLEDQKAFPDLVELSQLEDLLLEEKLTRVDDP
CCCCHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
FEYIVNLLPEPDSKETTIRNRNYQIIKPLVENPNFYIKKVRAKHVATILKSGEISRPYLY
HHHHHHHCCCCCCCCCEECCCCCEEEHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCHHHH
KLIRRYWQRGQVSNTLLPDYRNSGAKGQKRVANDKKLGRPRKIMEGTGALVDEPTEKLFR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCHHHHHCCCCCCCCHHHHHHH
IIIDKYIFNKQFSIARAHRKFKGLYDNIFPGVPESEKPTKRQLSYFLDREYKSVDKIKAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSDIIYKKDVRPLHSTATIQALGPGSRYEIDATIADVILVSDHDRNQPVGRPTVYIVIDV
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEHHHH
FSRLIVGWYIGFENPSYVAAIQSLYLALNDKKHLFKDLDIETDNFSWPTPGLPEAILADR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCC
GELIGHQIEGLESSYRVRIENTPPYRGDAKGIVEQRFRTLQAEFKKFTPGEVIGPTVRKR
HHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
GGKNYWLDGKLTISEFTEIIVSSIVMRNFVDPMSKYDRAKDMPANLPSIPIHLWNWGLQN
CCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCC
RTGRLRKANAESVRIALLPRNQASVSAQGICIFGVYYSGPEIVELGWMHRSDKSTRPKKV
CCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEECCEECCCCCCCCCEE
EVAFDPNFADEIYLFHTAGSREHWVCRITDLSREFRGQTFWEVWRKQKQIKMQGSKDQLH
EEEECCCCCCEEEEEEECCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHH
ADKLRKHHENRVEDIISSAIKQTPKQTATNVDRMKGVSYARSDQLAKERELRRPKRPERQ
HHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCH
QTAKVVHLTPPEDSDEYPDFEDELFDGGDS
HCCEEEEECCCCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]