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The map label for this gene is prpB [H]

Identifier: 126176237

GI number: 126176237

Start: 4756727

End: 4757590

Strand: Direct

Name: prpB [H]

Synonym: Sbal_4051

Alternate gene names: 126176237

Gene position: 4756727-4757590 (Clockwise)

Preceding gene: 126176236

Following gene: 126176238

Centisome position: 92.77

GC content: 49.07

Gene sequence:

>864_bases
ATGAAAAAAACAACGGCATTACGACAAATGCTCAGTGGTAGCGATATTCACGTCGCCCCAGGCGTTTACGATGGCATGAC
CGCACGTTTAGCCGAACAGGCGGGGTTTAAGCTGATTTATGCCAGTGGCGGCGCGATTGCGAGAAGTTGTGGTTTCCCCG
ATATAGGCATGCTGAGCTTTAGCGAAGTGCTGCATCGACTCGAACAGATGGTCGAAGTGACCCAAGTCCCCATCATAGCG
GATGCAGATACTGGGTTTGGTAATGCCATCAACGTGAGTCGCACAGTAAAAGCCTTTGAGCGAGCCGGTGTCGCAGCATT
GCACTTGGAAGATCAAACCTTTCCAAAGCGCTGCGGCCATCTGAATGATAAATCTTTGGTGAGTACCCAAGAAATGGTGC
ACAAAATCCGTGTTGCCAAGGATAGCCAAACTGACCCAGATTTTACCTTAATTGCCCGCACTGATGCGATCGCCGTTGAA
GGATTCGATGCTGCAATGGAACGCTCACATGCGTATTTAGCAGCGGGTGCCGACGTGATTTTTGTCGAAGCGCCCGAAAC
CATTGAGCAAATTGAATTGATCGCTAAGTATATTAAGCAGCCTAAGCTCATCAATATGTTCCACAGTGGTAAAACACCGC
TGGTGTCAAAGGACAGACTGCAAGCATTGGGATACAAGTTCATTATTATTCCCTCAGACTTACAGCGAGCGACTATCCAT
GCTTGTCAGCACACCTTACGCACCATTCTCGAGCAAGGTGACAGTGGCAGTATTGCCGAGCAGATGGTGTCGTTTGCAGA
GCGTGAACGGATCATAAACACACATGCGTATTTGGATCTTGACCTCGGCTATGGGCGTAACTGA

Upstream 100 bases:

>100_bases
GCGCCCGCGTGCTAATGGAAGGTTTTGTGCGAGTACCGAGCGAGAGTTTCTAATCGTTTATCTGGTTTTAAATTCAAGAC
GCACAAGGAAGAGGATTCAA

Downstream 100 bases:

>100_bases
GTCAATGCAAGTTTAACCCTGCCCTCTAAACGCTAAAACAGTTCAACCAAAGACCTTGCTTCTCCCCCTTCCGGCAAGGT
CTTTTTCTATTCGCCCTCAG

Product: isocitrate lyase family protein

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQAGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKYIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTHAYLDLDLGYGRN

Sequences:

>Translated_287_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQAGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKYIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTHAYLDLDLGYGRN
>Mature_287_residues
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQAGFKLIYASGGAIARSCGFPDIGMLSFSEVLHRLEQMVEVTQVPIIA
DADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGHLNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVE
GFDAAMERSHAYLAAGADVIFVEAPETIEQIELIAKYIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTHAYLDLDLGYGRN

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=274, Percent_Identity=39.7810218978102, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1790445, Length=89, Percent_Identity=46.0674157303371, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982926, Length=213, Percent_Identity=31.4553990610329, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=213, Percent_Identity=31.4553990610329, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31504; Mature: 31504

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQAGFKLIYASGGAIARSCGFPDIGMLSF
CCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCHHHH
SEVLHRLEQMVEVTQVPIIADADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGH
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCC
LNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVEGFDAAMERSHAYLAAGADVI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCHHHHHHHCCEEEEECCCEE
FVEAPETIEQIELIAKYIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
EEECCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTHAYLDLDLGYGRN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
>Mature Secondary Structure
MKKTTALRQMLSGSDIHVAPGVYDGMTARLAEQAGFKLIYASGGAIARSCGFPDIGMLSF
CCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCHHHH
SEVLHRLEQMVEVTQVPIIADADTGFGNAINVSRTVKAFERAGVAALHLEDQTFPKRCGH
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCC
LNDKSLVSTQEMVHKIRVAKDSQTDPDFTLIARTDAIAVEGFDAAMERSHAYLAAGADVI
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCHHHHHHHCCEEEEECCCEE
FVEAPETIEQIELIAKYIKQPKLINMFHSGKTPLVSKDRLQALGYKFIIIPSDLQRATIH
EEECCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHH
ACQHTLRTILEQGDSGSIAEQMVSFAERERIINTHAYLDLDLGYGRN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]