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The map label for this gene is mii [H]

Identifier: 126176236

GI number: 126176236

Start: 4755456

End: 4756679

Strand: Direct

Name: mii [H]

Synonym: Sbal_4050

Alternate gene names: 126176236

Gene position: 4755456-4756679 (Clockwise)

Preceding gene: 126176235

Following gene: 126176237

Centisome position: 92.75

GC content: 53.1

Gene sequence:

>1224_bases
ATGAATGCTTCAACACCCCACGCGCCGCAAATCAAAGTCGCCGCGACTTATATGCGCGGCGGCACCAGTAAAGGCGTGTT
TTTCCGTCTGCAAGATTTACCTAAAGCCGCACAAGTTGCTGGCCCTGCCCGTGATGCGCTACTACTGCGAGTGATTGGCA
GCCCAGATCCCTACGGCAAACAAATTGATGGCATGGGTGCTGCCACCTCAAGTACCAGTAAAACCGTGATCCTCTCCCCT
AGCACCCAAACCGATCACGATGTCGATTACCTGTTCGGCCAAGTGGCCATCGATAAACCCTTTGTCGATTGGAGTGGTAA
CTGCGGCAACTTAACCGCCGCCGTCGGCGCCTTTGCCATCAGCAATGGCTTAATTGATACGGCCCGTATTCCACAAAATG
GCATCTGCACTGTGCGTATTTGGCAAGCCAACATAGGCAAAACCATTATCGCCCATGTACCCATCACCAAGGGTGAAGTG
CAAGAAACCGGTGATTTTGAGCTCGATGGTGTGACCTTTCCCGCGGCCGAAGTGCAAATCGAATTTATGAACCCGGCTGC
TGACGAGGATGCCAATTCTAACGGAGGGAGTGGCAGCATGTTCCCGACCGGCAATGTTGTCGATGTGCTAGAAGTACCGG
GTATTGGCAGTTTTAATGCCACTATGATCAACGCTGGTATTCCCACCATTTTTATTAATGCAGAGGACTTAGGCTACACA
GGCACTGAGCTGCAGGATGATATTAACAGCGACACGGCGGCACTGGCTAAATTTGAAACCATTCGCGCCTATGGTGCAGT
GCGTATGGGCTTGATTTCGCATATAGATGAGGCCATTACACGCCAACATACGCCCAAGGTCGCCTTTGTTGCTAAACCCA
AAGCCTATGTGTCATCGAGCGGCAAACCCGTCAATACCGATGATATCGACTTGCTCGTGCGCGCACTGTCGATGGGCAAA
CTGCACCACGCCATGATGGGCACAGCAGCAGTGGCAATTGGCACAGCAGCAGCAATCCCCGGCACCTTAGTTAACTTAGC
CGCTGGCGGCGACACTAAGGGCAAAATAGACGCGGTGCGCTTTGGTCATCCCTCTGGCACGCTGCGTGTTGGGGCTGAGG
CGTCAATGGTCAACGGCGAATGGACAGTCGTTAAAGCCATTATGAGCCGCAGCGCCCGCGTGCTAATGGAAGGTTTTGTG
CGAGTACCGAGCGAGAGTTTCTAA

Upstream 100 bases:

>100_bases
CAGCTAAAGATAAGCTAGCGAAACCGAGCGCCAATAACTTCCCCAATTAAGTTACTGGCGCTCATTTGGATTCCATTGGG
AGTCCTTTTGGAGTCAATCT

Downstream 100 bases:

>100_bases
TCGTTTATCTGGTTTTAAATTCAAGACGCACAAGGAAGAGGATTCAAATGAAAAAAACAACGGCATTACGACAAATGCTC
AGTGGTAGCGATATTCACGT

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MNASTPHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDPYGKQIDGMGAATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDANSNGGSGSMFPTGNVVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAAGGDTKGKIDAVRFGHPSGTLRVGAEASMVNGEWTVVKAIMSRSARVLMEGFV
RVPSESF

Sequences:

>Translated_407_residues
MNASTPHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDPYGKQIDGMGAATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDANSNGGSGSMFPTGNVVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAAGGDTKGKIDAVRFGHPSGTLRVGAEASMVNGEWTVVKAIMSRSARVLMEGFV
RVPSESF
>Mature_407_residues
MNASTPHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDPYGKQIDGMGAATSSTSKTVILSP
STQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAISNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEV
QETGDFELDGVTFPAAEVQIEFMNPAADEDANSNGGSGSMFPTGNVVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYT
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSSGKPVNTDDIDLLVRALSMGK
LHHAMMGTAAVAIGTAAAIPGTLVNLAAGGDTKGKIDAVRFGHPSGTLRVGAEASMVNGEWTVVKAIMSRSARVLMEGFV
RVPSESF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=393, Percent_Identity=33.8422391857506, Blast_Score=168, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42560; Mature: 42560

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASTPHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDPYGK
CCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEEECCCCCCCC
QIDGMGAATSSTSKTVILSPSTQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAI
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHH
SNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEVQETGDFELDGVTFPAAEVQI
HCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEECCEECCCEEEEE
EFMNPAADEDANSNGGSGSMFPTGNVVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYT
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEECHHCCCC
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSS
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCC
GKPVNTDDIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGDTKGKIDAVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
FGHPSGTLRVGAEASMVNGEWTVVKAIMSRSARVLMEGFVRVPSESF
ECCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNASTPHAPQIKVAATYMRGGTSKGVFFRLQDLPKAAQVAGPARDALLLRVIGSPDPYGK
CCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEEECCCCCCCC
QIDGMGAATSSTSKTVILSPSTQTDHDVDYLFGQVAIDKPFVDWSGNCGNLTAAVGAFAI
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHH
SNGLIDTARIPQNGICTVRIWQANIGKTIIAHVPITKGEVQETGDFELDGVTFPAAEVQI
HCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCEEECCEECCCEEEEE
EFMNPAADEDANSNGGSGSMFPTGNVVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYT
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEECHHCCCC
GTELQDDINSDTAALAKFETIRAYGAVRMGLISHIDEAITRQHTPKVAFVAKPKAYVSSS
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCC
GKPVNTDDIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGDTKGKIDAVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
FGHPSGTLRVGAEASMVNGEWTVVKAIMSRSARVLMEGFVRVPSESF
ECCCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA