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The map label for this gene is prpB [H]

Identifier: 126176233

GI number: 126176233

Start: 4750315

End: 4751193

Strand: Direct

Name: prpB [H]

Synonym: Sbal_4047

Alternate gene names: 126176233

Gene position: 4750315-4751193 (Clockwise)

Preceding gene: 126176232

Following gene: 126176234

Centisome position: 92.65

GC content: 50.51

Gene sequence:

>879_bases
ATGACCCAAAGCGCAGGATTACGTTTTCGCCAAGCTCTTGCTAAGGCTAAACCGTTACAAATCGTTGGCACCACCAATGC
CTATTTTGCCTTGATGGCAGAGCAAACTGGCTTCCAAGCCTTGTATCTTTCGGGCGCTGGCGTTGCCAATGCTTCATACG
GTTTGCCTGACTTAGGCATGACCTCAATGAACGACGTGCTGATCGATGCCGGTCGTATCACCTCAGCCACACAAGTGCCT
TTATTGGTCGATATCGATACTGGCTGGGGCGGCGCATTCAACATTGCTCGCACCATCAAAGAATTCGAAAAAGTCGGCGT
CGCCGCTGTGCACATGGAAGACCAAGTGTCGCAAAAGCGTTGCGGCCACAGACCTAACAAAGCGGTCGTGAGCATAGAAG
AAATGGTCGATCGCATTAAAGCGGCCGTCGATGCCCGTACCGATCCTAACTTTGTGATCATGGCGCGTACCGATGCAGTT
GCCGTTGAAGGCTTAGAAGCCGGTATCGAACGCGCGCAAGCCTATATCGCCGCCGGTGCCGATATGATCTTCGCCGAAGC
CTTAACTGAGCTAGACCAATATCGCCACTTTAAAGCGCAAGTCAAAGCACCGATTTTGGCCAACATGACTGAATTTGGTC
AAACCCAATTGTTCAATAAAGAAGAACTCGCGCAGGCAGGCGCCGACATGGTGCTCTATCCACTCGGTACTTTCCGTGCA
GCCAACCAAGCGGCACTGAACGTTATGCAAGCGCTGATGAATGATGGTCATCAACGCAACGTACTCGACACTATGCAGAC
TCGTAAAGACTTGTATAAGTACTTGGGCTACCACGCCTTTGAAGACAAGTTAGATCAACTGTTTAGCCAAGATAAATAG

Upstream 100 bases:

>100_bases
AGCCAATGCAGCCGCAAAAGAAACCTTAGCCAACATCAGCTAAGTTTATACGGCTCAATATGCCCACTTCCCATAAATGG
AAAAGGATAAGGACAAGACC

Downstream 100 bases:

>100_bases
CTAAACAAACGAGAAAGCAAAATACACAAGACCATGGATTGTCAGTAGAAGCGCAAGCTCATGTTGTTACTGAAACCCAG
ACGCTTTGCCCCAAGCAGCA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVP
LLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAV
AVEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAQAGADMVLYPLGTFRA
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK

Sequences:

>Translated_292_residues
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVP
LLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAV
AVEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAQAGADMVLYPLGTFRA
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
>Mature_291_residues
TQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQVPL
LVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVA
VEGLEAGIERAQAYIAAGADMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAQAGADMVLYPLGTFRAA
NQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=60.1374570446735, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790445, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320908, Length=222, Percent_Identity=27.9279279279279, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31934; Mature: 31803

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGM
CCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQVPLLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVAVEGLEAGIERAQAYIAAGA
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCH
DMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAQAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCHHHH
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQSAGLRFRQALAKAKPLQIVGTTNAYFALMAEQTGFQALYLSGAGVANASYGLPDLGM
CCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQVPLLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSIEEMVDRIKAAVDARTDPNFVIMARTDAVAVEGLEAGIERAQAYIAAGA
CCCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCH
DMIFAEALTELDQYRHFKAQVKAPILANMTEFGQTQLFNKEELAQAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCHHHH
ANQAALNVMQALMNDGHQRNVLDTMQTRKDLYKYLGYHAFEDKLDQLFSQDK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]