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The map label for this gene is prpC [H]

Identifier: 126176234

GI number: 126176234

Start: 4751469

End: 4752596

Strand: Direct

Name: prpC [H]

Synonym: Sbal_4048

Alternate gene names: 126176234

Gene position: 4751469-4752596 (Clockwise)

Preceding gene: 126176233

Following gene: 126176235

Centisome position: 92.67

GC content: 51.86

Gene sequence:

>1128_bases
ATGTCAGAAGCTAAAAAACTAAGTGGTGCAGGATTACGTGGCCAGAGCGCAGGCGAAACGGCATTGAGCACAGTCGGCGT
GTCAGGCTCAGGCCTCACGTACCGCGGTTATGATGTGAAAGATCTCGGTGAAAATGCCACCTTCGAAGAAGTCGCCTATC
TCATTCTTTACGGCGAGCTGCCCACTCAAGCCCAGCTTGATGCCTACAAAACCAAACTCAAAGGCCTTCGCGGTCTGCCA
CAAGCATTAAAAGAAGTATTAGAGCGCATTCCTGCCGAGACTCATCCTATGGATGTAATGCGTACTGGCTGCTCTATGCT
AGGCAACTTAGAAGCCGAACACAGCTTTGCCGAACAGAGTGAAATCACCGACCGCCTATTGGCTGCATTCCCATCGATCA
TCTGTTACTGGTATCGCTTTAGTCACGATGGCGTGCGTATCGACACTGAAACCGATGACGATCAAATTGGCGCGCACTTC
CTGCATTTATTACACGGTAAAGCGCCGTCGGCACTGCATGCTAAAGTGATGGACGTATCGTTAATTCTTTATGCAGAACA
TGAATTTAACGCATCAACCTTTACCGCTCGCGTATGTGCTTCAACTCTGTCAGACATGCATTCATGCGTCACTGGCGCCA
TTGGTTCACTGCGCGGCCCGCTGCATGGCGGCGCGAACGAAGCGGCGATGGAACTGATCCAAGACATGAAAGATGAAGCC
GATGCGCGCGACATCTTGATGGGCAAACTGGAGCGTAAAGATAAGATCATGGGCTTTGGCCACGCGATTTACCGCGACAG
CGATCCACGTAATGCCATCATCAAAGAATGGTCAGAAAAACTGGCTGCCGCCAACGGTGACGACCGTCTATACCGCGTAT
CGGTTGCCTGTGAAGCCCTGATGTGGGAACAGAAAAAACTCTTCTGTAATGCAGATTTTTTCCACGCCAGCGCGTACCAC
TACATGGACATTCCGACTAAGTTGTTCACGCCAATCTTCGTGTGTTCACGGGTAACGGGCTGGGCGGCGCACGTGATGGA
GCAACGCTCTAACAACCGCATCATCCGCCCAAGTGCCGATTATGTGGGCGTGGCGCCACGCAAAGTCGTCCCCATTGCCA
ACCGTTAA

Upstream 100 bases:

>100_bases
AGTGCAGCCTTAACCAATAAATGTCGGCGAATGAACACTCAAAAATGAGTGCATTCATCCACCAGCAATAACCACACAGA
GTTAACGGAAGGGAAGGATT

Downstream 100 bases:

>100_bases
GCCAAGCTAACGACTCTGGCGCGAGACTTCTGTTAAAGAAATGTCTGTGAATAGACAGTCTCGCGCCAACTTCATTTTCC
GAAATGGATGATGTTATGAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSEAKKLSGAGLRGQSAGETALSTVGVSGSGLTYRGYDVKDLGENATFEEVAYLILYGELPTQAQLDAYKTKLKGLRGLP
QALKEVLERIPAETHPMDVMRTGCSMLGNLEAEHSFAEQSEITDRLLAAFPSIICYWYRFSHDGVRIDTETDDDQIGAHF
LHLLHGKAPSALHAKVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMKDEA
DARDILMGKLERKDKIMGFGHAIYRDSDPRNAIIKEWSEKLAAANGDDRLYRVSVACEALMWEQKKLFCNADFFHASAYH
YMDIPTKLFTPIFVCSRVTGWAAHVMEQRSNNRIIRPSADYVGVAPRKVVPIANR

Sequences:

>Translated_375_residues
MSEAKKLSGAGLRGQSAGETALSTVGVSGSGLTYRGYDVKDLGENATFEEVAYLILYGELPTQAQLDAYKTKLKGLRGLP
QALKEVLERIPAETHPMDVMRTGCSMLGNLEAEHSFAEQSEITDRLLAAFPSIICYWYRFSHDGVRIDTETDDDQIGAHF
LHLLHGKAPSALHAKVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMKDEA
DARDILMGKLERKDKIMGFGHAIYRDSDPRNAIIKEWSEKLAAANGDDRLYRVSVACEALMWEQKKLFCNADFFHASAYH
YMDIPTKLFTPIFVCSRVTGWAAHVMEQRSNNRIIRPSADYVGVAPRKVVPIANR
>Mature_374_residues
SEAKKLSGAGLRGQSAGETALSTVGVSGSGLTYRGYDVKDLGENATFEEVAYLILYGELPTQAQLDAYKTKLKGLRGLPQ
ALKEVLERIPAETHPMDVMRTGCSMLGNLEAEHSFAEQSEITDRLLAAFPSIICYWYRFSHDGVRIDTETDDDQIGAHFL
HLLHGKAPSALHAKVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMKDEAD
ARDILMGKLERKDKIMGFGHAIYRDSDPRNAIIKEWSEKLAAANGDDRLYRVSVACEALMWEQKKLFCNADFFHASAYHY
MDIPTKLFTPIFVCSRVTGWAAHVMEQRSNNRIIRPSADYVGVAPRKVVPIANR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=377, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=52.4064171122995, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786939, Length=378, Percent_Identity=29.6296296296296, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17555174, Length=354, Percent_Identity=24.2937853107345, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=372, Percent_Identity=24.1935483870968, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=281, Percent_Identity=24.9110320284698, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24640126, Length=379, Percent_Identity=22.9551451187335, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640124, Length=379, Percent_Identity=22.9551451187335, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356863, Length=362, Percent_Identity=22.0994475138122, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 41421; Mature: 41289

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKKLSGAGLRGQSAGETALSTVGVSGSGLTYRGYDVKDLGENATFEEVAYLILYGEL
CCCCHHCCCCCCCCCCCCCHHHHHCCCCCCCCEECCCCHHHCCCCCCHHHHEEEEEECCC
PTQAQLDAYKTKLKGLRGLPQALKEVLERIPAETHPMDVMRTGCSMLGNLEAEHSFAEQS
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EITDRLLAAFPSIICYWYRFSHDGVRIDTETDDDQIGAHFLHLLHGKAPSALHAKVMDVS
HHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHEEEE
LILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMKDEA
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
DARDILMGKLERKDKIMGFGHAIYRDSDPRNAIIKEWSEKLAAANGDDRLYRVSVACEAL
CHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH
MWEQKKLFCNADFFHASAYHYMDIPTKLFTPIFVCSRVTGWAAHVMEQRSNNRIIRPSAD
HHHHHHHEECCCHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
YVGVAPRKVVPIANR
CCCCCCCEECCCCCC
>Mature Secondary Structure 
SEAKKLSGAGLRGQSAGETALSTVGVSGSGLTYRGYDVKDLGENATFEEVAYLILYGEL
CCCHHCCCCCCCCCCCCCHHHHHCCCCCCCCEECCCCHHHCCCCCCHHHHEEEEEECCC
PTQAQLDAYKTKLKGLRGLPQALKEVLERIPAETHPMDVMRTGCSMLGNLEAEHSFAEQS
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EITDRLLAAFPSIICYWYRFSHDGVRIDTETDDDQIGAHFLHLLHGKAPSALHAKVMDVS
HHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHEEEE
LILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMKDEA
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
DARDILMGKLERKDKIMGFGHAIYRDSDPRNAIIKEWSEKLAAANGDDRLYRVSVACEAL
CHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH
MWEQKKLFCNADFFHASAYHYMDIPTKLFTPIFVCSRVTGWAAHVMEQRSNNRIIRPSAD
HHHHHHHEECCCHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
YVGVAPRKVVPIANR
CCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]