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The map label for this gene is ilvG [H]

Identifier: 126176217

GI number: 126176217

Start: 4731121

End: 4732794

Strand: Reverse

Name: ilvG [H]

Synonym: Sbal_4031

Alternate gene names: 126176217

Gene position: 4732794-4731121 (Counterclockwise)

Preceding gene: 126176218

Following gene: 126176216

Centisome position: 92.3

GC content: 53.76

Gene sequence:

>1674_bases
ATGGAACCAGGGCAGATGATCAGAGGCGCAGATGCGGTAATTAAAGTGTTAGCAGCACATGGCGTCACCACAGTGTTTGG
TTACCCAGGTGGTGCCATTATGCCAATATACGATGCCCTCTATGGAAGCCCTGTCGAACATCTGCTCAGCCGTCACGAAC
AAGGCGCCGCCTTTGCCGCCGTAGGATACGCAAGAGCCAGCGGTAAAACTGGTGTGTGCTTTGCGACTTCAGGCCCTGGC
GCGACTAACTTGATCACTTCCTTAGCCGATGCCCTGTTAGATTCAGTGCCTATGGTGGCCATCACAGGCCAAGTCTCTAC
CGCCGTTATCGGCACCGACGCCTTTCAAGAAATCGACGTGCTGGGCATGTCACTGAGCTGCACTAAGCACAGTTTTATGG
TGACAGACGTCAACGACCTGATCCCAACCTTGTATCAAGCCTTCGAAATCGCCGCATCTGGTCGCCCTGGCCCTGTGCTG
GTCGATATCCCGAAGGATATCCAAATCGCCCAATTGGAATATCGCACACCTTTGCTGGCCGTGATGAACGAACCCCAAGC
CGAGATGAGTGATATCGACGCTGCCCGTGCACTGTTGGCTGAGGCTAAACAGCCTATGTTGTATGTCGGCGGTGGTGTGG
GCATGGCGGGCGCGGTCGATCAACTGCGTGAGTTTATCAAAGTGACGGGCATACCTTCGGTCGCAACCTTGAAAGGGCTG
GGTAGTATCGCCCACGGCACATCCGGCTACTTAGGCATGTTAGGCATGCATGGCGGCAAGGCAGCTAACTTAGCGGTACA
GGATTGCGATTTGCTGATCGTGGTGGGTGCCCGTTTCGACGACCGAGTCACCGGCCGTTTAGCGAGCTTTGCCGACAAGG
CCAAAGTGATCCATTTAGACATCGACGCCGCCGAACTGGGTAAATTGCGTATGCCTGAGGTGGCAATCGCTGGTGACTTA
CGCCAAATCTTACCTGCCTTAGCCATGTCGATGAACATCACGCCTTGGCAGGCCGAGGTGGAGCATTTATCCCGTAAGCA
TCAATGGGATTATCAACACCCCGGCAGTTTGATCTATGCGCCTGCGCTGCTGCGCCGCTTAGCCAATAAATTACCCGAAG
ACAGCGTAGTGTGCTGCGATGTGGGTCAGCACCAAATGTGGGTGGCCCAGCACATGTGGTTCCGCCGCCCAGAAGATCAT
CTTTCCAGCGCGGGCTTAGGCACTATGGGCTTTGGCTTACCCGCCGCGATTGGTGCGCAAGTGGCCCGTCCCGATGCGAC
TGTGGTGACTGTGTCTGGCGATGGGTCTTTTATGATGAATGTGCAGGAACTGACCACCATCAAACGCCGCAAGCTGCCAG
TGAAAATCGTGCTGGTTGATAACCAAAAGCTTGGCATGGTGAAACAGTGGCAACAGCTATTCTTTGAAGAACGCTACAGC
GAAACGGATTTATCCGATAACCCAGATTTTGTTTTATTGGCCTCCGCCTTTGATATCCCCGGCCGCACGATTTTCTCTTC
CGACGAAGTGGAAGAAGCATTAACCGAAATGTTAGCGGCTAAGGGCCCGTATTTATTACACGTTGCAATCGACGATGCTT
TTAACGTTTGGCCACTGGTGCCCCCCGGCGCATCAAACAGCGATATGATGGACGAAATGGAGAAACAAACATGA

Upstream 100 bases:

>100_bases
GTTCTTCGTTTCAGGGCCTAGAGCAAGGTTCTAAGTTTCGAAAGAGTAAAAAATTAGCGTCGATTTTCGGCAATTCAGGA
CTAACGTAGCGAGAGAGAAT

Downstream 100 bases:

>100_bases
TCCACTCTCTGGAATTAACGGTTCAACAACGCCCCGAAGTGCTTGAGCGTGTTTTGCGCGTGACTCGCCATCGTGGCTTT
ACGATTACCCAAATGCAGAT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPMVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVMNEPQAEMSDIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKVTGIPSVATLKGL
GSIAHGTSGYLGMLGMHGGKAANLAVQDCDLLIVVGARFDDRVTGRLASFADKAKVIHLDIDAAELGKLRMPEVAIAGDL
RQILPALAMSMNITPWQAEVEHLSRKHQWDYQHPGSLIYAPALLRRLANKLPEDSVVCCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKIVLVDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVLLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT

Sequences:

>Translated_557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPMVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVMNEPQAEMSDIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKVTGIPSVATLKGL
GSIAHGTSGYLGMLGMHGGKAANLAVQDCDLLIVVGARFDDRVTGRLASFADKAKVIHLDIDAAELGKLRMPEVAIAGDL
RQILPALAMSMNITPWQAEVEHLSRKHQWDYQHPGSLIYAPALLRRLANKLPEDSVVCCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKIVLVDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVLLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT
>Mature_557_residues
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGKTGVCFATSGPG
ATNLITSLADALLDSVPMVAITGQVSTAVIGTDAFQEIDVLGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVL
VDIPKDIQIAQLEYRTPLLAVMNEPQAEMSDIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKVTGIPSVATLKGL
GSIAHGTSGYLGMLGMHGGKAANLAVQDCDLLIVVGARFDDRVTGRLASFADKAKVIHLDIDAAELGKLRMPEVAIAGDL
RQILPALAMSMNITPWQAEVEHLSRKHQWDYQHPGSLIYAPALLRRLANKLPEDSVVCCDVGQHQMWVAQHMWFRRPEDH
LSSAGLGTMGFGLPAAIGAQVARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKIVLVDNQKLGMVKQWQQLFFEERYS
ETDLSDNPDFVLLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLVPPGASNSDMMDEMEKQT

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=518, Percent_Identity=27.992277992278, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=24.8214285714286, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=44.1818181818182, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=41.8277680140597, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=30.2491103202847, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=27.9850746268657, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=27.2072072072072, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=525, Percent_Identity=28, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=27.4226804123711, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=27.4226804123711, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=42.9794520547945, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=21.875, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=478, Percent_Identity=24.0585774058577, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=470, Percent_Identity=24.2553191489362, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=481, Percent_Identity=22.8690228690229, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922626, Length=504, Percent_Identity=27.1825396825397, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59753; Mature: 59753

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAA
CCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEE
VGYARASGKTGVCFATSGPGATNLITSLADALLDSVPMVAITGQVSTAVIGTDAFQEIDV
EEEHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
LGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVLVDIPKDIQIAQLEYRTPLLA
HCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCE
VMNEPQAEMSDIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKVTGIPSVATLKGL
ECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHC
GSIAHGTSGYLGMLGMHGGKAANLAVQDCDLLIVVGARFDDRVTGRLASFADKAKVIHLD
CHHCCCCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEE
IDAAELGKLRMPEVAIAGDLRQILPALAMSMNITPWQAEVEHLSRKHQWDYQHPGSLIYA
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEH
PALLRRLANKLPEDSVVCCDVGQHQMWVAQHMWFRRPEDHLSSAGLGTMGFGLPAAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHCCH
VARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKIVLVDNQKLGMVKQWQQLFFEERYS
HCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
ETDLSDNPDFVLLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLV
CCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PPGASNSDMMDEMEKQT
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEPGQMIRGADAVIKVLAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAA
CCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEE
VGYARASGKTGVCFATSGPGATNLITSLADALLDSVPMVAITGQVSTAVIGTDAFQEIDV
EEEHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHH
LGMSLSCTKHSFMVTDVNDLIPTLYQAFEIAASGRPGPVLVDIPKDIQIAQLEYRTPLLA
HCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCE
VMNEPQAEMSDIDAARALLAEAKQPMLYVGGGVGMAGAVDQLREFIKVTGIPSVATLKGL
ECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHC
GSIAHGTSGYLGMLGMHGGKAANLAVQDCDLLIVVGARFDDRVTGRLASFADKAKVIHLD
CHHCCCCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEE
IDAAELGKLRMPEVAIAGDLRQILPALAMSMNITPWQAEVEHLSRKHQWDYQHPGSLIYA
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEH
PALLRRLANKLPEDSVVCCDVGQHQMWVAQHMWFRRPEDHLSSAGLGTMGFGLPAAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHCCH
VARPDATVVTVSGDGSFMMNVQELTTIKRRKLPVKIVLVDNQKLGMVKQWQQLFFEERYS
HCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
ETDLSDNPDFVLLASAFDIPGRTIFSSDEVEEALTEMLAAKGPYLLHVAIDDAFNVWPLV
CCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PPGASNSDMMDEMEKQT
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]