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The map label for this gene is ilvA [H]

Identifier: 126176214

GI number: 126176214

Start: 4727259

End: 4728854

Strand: Reverse

Name: ilvA [H]

Synonym: Sbal_4028

Alternate gene names: 126176214

Gene position: 4728854-4727259 (Counterclockwise)

Preceding gene: 126176215

Following gene: 126176213

Centisome position: 92.23

GC content: 51.75

Gene sequence:

>1596_bases
ATGTTGCCACTTGCATCGCAAGCTCGGACGGATTTAGCACATTTTCAGTTGGCACAAAGCTATCTGCAAAAAATCCTGTT
GTCATCTGTGTATGACATAGCGAAAGTGACGCCGCTTTCAAGCATGAACAAGTTATCGGCGCGTTTAGGTTGTCAGGTAT
TTTTGAAGCGCGAAGATATGCAGCCAGTGCATTCCTTCAAGTTACGCGGTGCCTATAACCGTATCGCCCAGCTGACGAAA
GAAGAATGTCAGCGCGGCGTGGTGTGCGCTTCGGCAGGCAATCATGCTCAGGGTGTGGCGATGTCGGCGGCGAGTCGCGG
CGTGGATGCTGTAATTGTGATGCCTGAGACGACGCCCGATATTAAAGTCGATGCGGTGCGCCGTTTAGGTGGCAACGTGG
TATTGCACGGCCAAGCCTTCGATCAAGCCAATGGCTATGCGATGGCGATGGCTAAAGAAGAGGGCCGAGTCTATATCGCG
CCCTTTGATGATGAAGCAGTGATCGCAGGCCAAGGCACTATCGCCCAAGAGATGTTGCAGCAGCAGCGCGATCTCGAAGT
GGTATTTGTGCCCGTGGGCGGCGGCGGTTTAATCGCGGGTATCGCAGCCTATTACAAGGCGGTGATGCCGCAGGTGAAAA
TTGTCGGCGTCGAGCCAGAAGACGCTGCCTGTTTAAAGGCGGCGATGGAAGCGGGCGAACCTGTGACACTGGCGCAAGTC
GGTTTATTTGCCGACGGTGTGGCGGTAAAGCGTATCGGTACTGAGCCGTTTCGAGTCGCCAAACTGTATGTCGATGAAGT
AGTCACCGTCACCTCGGATGAAATCTGCGCTGCGGTGAAAGATATCTTCGAAGATACCCGTGCGATTGCTGAACCCGCTG
GGGCTTTGTCCTTAGCAGGGCTTAAAAAATATGTCAGCGCTAATGACAGTGGCGACAGCGGTAAAGGCGAGAAAGTCGCC
GCGATTTTGAGTGGCGCGAATGTGAATTTCCACAGCCTGTGTTATGTGTCTGAACGCTGCGAACTCGGTGAGCAAAAAGA
AGCAGTACTGGCAGTGAAAGTCCCTGAACGTCCGGGAAGTTTCTTAAAATTCTGTGAGTTACTCGATAAGCGGGTGATGA
CAGAGTTCAACTATCGTTTCAGCAGTCGCGATCTGGCCGTGGTGTTTGCTGGCATTCGTTTAAGCCGAGGCCAAGGCGAG
CTTGAACAAATCATCGCTACCTTAGAGGCTAACGATTTTGAAGTGCAGGATTTATCTGGCGATGAAACCGCGAAATTGCA
TGTGCGCTACATGGTCGGCGGTCATCCACCTGAGCCTTTAGAAGAGCGTTTGTTTAGCTTCGAATTCCCCGAACATCCTG
GTGCGCTGCTGAAGTTTTTAACTACCTTGCAGAGCAAATGGAACATCAGTCTGTTCCATTATCGCAACCACGGCGCGGCT
TTTGGCCGAGTGCTGGCGGGATTTGAAGTACCCGCGAGTGATGCTTTGCCGTTCCAACAGTTTTTAACTGAGTTGGGTTT
TGTCTATCAAGAGGAAACCCAAAGTCCTGCCTATCAGCTGTTTTTAAATGCGGGTAACGGCAGCAAAGTCTTATAG

Upstream 100 bases:

>100_bases
CGCCAACGTTATGTATCACTCGCGCTTAAGGCCTACGCCATGCTCGCCACCAGTGCCGACAAGGGCGCGGTGCGCGATCG
CAGTAAACTGGAGGACTAAT

Downstream 100 bases:

>100_bases
GGTTTGCGGTGATGCACGTAGCTTAGATCGACGCGATGACATGTTAGGCAAAGTTAGGCACAAGTTAAGTCAAAGTTAGG
CTAAAGGAGGGGCGCACAGG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MLPLASQARTDLAHFQLAQSYLQKILLSSVYDIAKVTPLSSMNKLSARLGCQVFLKREDMQPVHSFKLRGAYNRIAQLTK
EECQRGVVCASAGNHAQGVAMSAASRGVDAVIVMPETTPDIKVDAVRRLGGNVVLHGQAFDQANGYAMAMAKEEGRVYIA
PFDDEAVIAGQGTIAQEMLQQQRDLEVVFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAAMEAGEPVTLAQV
GLFADGVAVKRIGTEPFRVAKLYVDEVVTVTSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYVSANDSGDSGKGEKVA
AILSGANVNFHSLCYVSERCELGEQKEAVLAVKVPERPGSFLKFCELLDKRVMTEFNYRFSSRDLAVVFAGIRLSRGQGE
LEQIIATLEANDFEVQDLSGDETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAA
FGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGNGSKVL

Sequences:

>Translated_531_residues
MLPLASQARTDLAHFQLAQSYLQKILLSSVYDIAKVTPLSSMNKLSARLGCQVFLKREDMQPVHSFKLRGAYNRIAQLTK
EECQRGVVCASAGNHAQGVAMSAASRGVDAVIVMPETTPDIKVDAVRRLGGNVVLHGQAFDQANGYAMAMAKEEGRVYIA
PFDDEAVIAGQGTIAQEMLQQQRDLEVVFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAAMEAGEPVTLAQV
GLFADGVAVKRIGTEPFRVAKLYVDEVVTVTSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYVSANDSGDSGKGEKVA
AILSGANVNFHSLCYVSERCELGEQKEAVLAVKVPERPGSFLKFCELLDKRVMTEFNYRFSSRDLAVVFAGIRLSRGQGE
LEQIIATLEANDFEVQDLSGDETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAA
FGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGNGSKVL
>Mature_531_residues
MLPLASQARTDLAHFQLAQSYLQKILLSSVYDIAKVTPLSSMNKLSARLGCQVFLKREDMQPVHSFKLRGAYNRIAQLTK
EECQRGVVCASAGNHAQGVAMSAASRGVDAVIVMPETTPDIKVDAVRRLGGNVVLHGQAFDQANGYAMAMAKEEGRVYIA
PFDDEAVIAGQGTIAQEMLQQQRDLEVVFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAAMEAGEPVTLAQV
GLFADGVAVKRIGTEPFRVAKLYVDEVVTVTSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYVSANDSGDSGKGEKVA
AILSGANVNFHSLCYVSERCELGEQKEAVLAVKVPERPGSFLKFCELLDKRVMTEFNYRFSSRDLAVVFAGIRLSRGQGE
LEQIIATLEANDFEVQDLSGDETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAA
FGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGNGSKVL

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=337, Percent_Identity=32.0474777448071, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=32.9032258064516, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI33469958, Length=272, Percent_Identity=29.7794117647059, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI295821202, Length=225, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI295821200, Length=225, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4557415, Length=225, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790207, Length=503, Percent_Identity=59.8409542743539, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789505, Length=306, Percent_Identity=37.5816993464052, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789235, Length=268, Percent_Identity=25.7462686567164, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI2367138, Length=206, Percent_Identity=27.1844660194175, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=380, Percent_Identity=33.1578947368421, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17537387, Length=328, Percent_Identity=31.7073170731707, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320930, Length=533, Percent_Identity=49.343339587242, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322631, Length=296, Percent_Identity=36.1486486486487, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6319788, Length=297, Percent_Identity=25.2525252525253, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321594, Length=215, Percent_Identity=28.3720930232558, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=35.1097178683386, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24645328, Length=255, Percent_Identity=34.9019607843137, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643623, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20129101, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 57578; Mature: 57578

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLASQARTDLAHFQLAQSYLQKILLSSVYDIAKVTPLSSMNKLSARLGCQVFLKREDM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCC
QPVHSFKLRGAYNRIAQLTKEECQRGVVCASAGNHAQGVAMSAASRGVDAVIVMPETTPD
CHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCC
IKVDAVRRLGGNVVLHGQAFDQANGYAMAMAKEEGRVYIAPFDDEAVIAGQGTIAQEMLQ
CCHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCEEEECCCHHHHHHHH
QQRDLEVVFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAAMEAGEPVTLAQV
HCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCEEEHHH
GLFADGVAVKRIGTEPFRVAKLYVDEVVTVTSDEICAAVKDIFEDTRAIAEPAGALSLAG
HHHHCCEEEEECCCCCHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKKYVSANDSGDSGKGEKVAAILSGANVNFHSLCYVSERCELGEQKEAVLAVKVPERPGS
HHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHEEEEHHHHCCCCCCCEEEEEECCCCCCH
FLKFCELLDKRVMTEFNYRFSSRDLAVVFAGIRLSRGQGELEQIIATLEANDFEVQDLSG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCC
DETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAA
CCEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHH
FGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGNGSKVL
HHHHHHCCCCCCCCCCCHHHHHHHHCCEEHHCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MLPLASQARTDLAHFQLAQSYLQKILLSSVYDIAKVTPLSSMNKLSARLGCQVFLKREDM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCC
QPVHSFKLRGAYNRIAQLTKEECQRGVVCASAGNHAQGVAMSAASRGVDAVIVMPETTPD
CHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCC
IKVDAVRRLGGNVVLHGQAFDQANGYAMAMAKEEGRVYIAPFDDEAVIAGQGTIAQEMLQ
CCHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCEEEECCCHHHHHHHH
QQRDLEVVFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAAMEAGEPVTLAQV
HCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCEEEHHH
GLFADGVAVKRIGTEPFRVAKLYVDEVVTVTSDEICAAVKDIFEDTRAIAEPAGALSLAG
HHHHCCEEEEECCCCCHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKKYVSANDSGDSGKGEKVAAILSGANVNFHSLCYVSERCELGEQKEAVLAVKVPERPGS
HHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHEEEEHHHHCCCCCCCEEEEEECCCCCCH
FLKFCELLDKRVMTEFNYRFSSRDLAVVFAGIRLSRGQGELEQIIATLEANDFEVQDLSG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCC
DETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWNISLFHYRNHGAA
CCEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHH
FGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGNGSKVL
HHHHHHCCCCCCCCCCCHHHHHHHHCCEEHHCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]