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The map label for this gene is ampD [H]

Identifier: 126176106

GI number: 126176106

Start: 4610206

End: 4610769

Strand: Reverse

Name: ampD [H]

Synonym: Sbal_3916

Alternate gene names: 126176106

Gene position: 4610769-4610206 (Counterclockwise)

Preceding gene: 126176117

Following gene: 126176105

Centisome position: 89.92

GC content: 47.16

Gene sequence:

>564_bases
ATGCGGTTTGAATTGGGTTGGTGTGAGGGTGCAAGGCGTTGTGAGTCACCACATTTTAATCAACGTCCCTTGGGTGAAGT
GAGTTTGTTGGTGATCCACAATATTAGCCTGCCAGCAGGTTGCTTTGGATTACCTCATATTGATCAATTATTTCAAGGGT
GCTTAGATACTAATGCCGATGCCAGTTTTGCACAGCTGCAGGGCTTGGAAGTATCGGCGCACTTTTTGATCCGCCGCAAT
GGCGAGCGTGTGCAGTACGTAAGTTGTAACGATAGGGCTTGGCATGCAGGGGTGTCTTGTTATGGCGAGAGAGAGAATTG
TAATGATTTCTCCATTGGTATTGAGCTGGAAGGCACAGATACTGAGCCTTACACGCCTGAGCAATATCAGTCACTGACAG
CGTTGACGCAAGCACTATTGGCTGAGTATCCATCGCTGAGTACCGAACGAATTGTGGGTCATTGTGATATTGCGCCAATG
CGCAAAACAGACCCTGGGCAAAGTTTTGATTGGGAAAGATATTTAGGGAAGCTAAAGGAATATACTCAGGCGTTGCGCTC
TTAG

Upstream 100 bases:

>100_bases
GGTCCGCAAATAACCGCCTTGCCTAAAGTGTGTTGCATTCCCGCTGAATCCTGCATCTTCAGGTTGTTTGGGTATATGTG
GCATAGAGGATAAGCTCATA

Downstream 100 bases:

>100_bases
GTTTTTGGATTTTAGACGTATTTCACACAAACCTAGACTATTTATGGACAAATTATAGAGGTACAGCCCATGGCATTATT
TTCACTCTTGGCCGCCATTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MRFELGWCEGARRCESPHFNQRPLGEVSLLVIHNISLPAGCFGLPHIDQLFQGCLDTNADASFAQLQGLEVSAHFLIRRN
GERVQYVSCNDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYTPEQYQSLTALTQALLAEYPSLSTERIVGHCDIAPM
RKTDPGQSFDWERYLGKLKEYTQALRS

Sequences:

>Translated_187_residues
MRFELGWCEGARRCESPHFNQRPLGEVSLLVIHNISLPAGCFGLPHIDQLFQGCLDTNADASFAQLQGLEVSAHFLIRRN
GERVQYVSCNDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYTPEQYQSLTALTQALLAEYPSLSTERIVGHCDIAPM
RKTDPGQSFDWERYLGKLKEYTQALRS
>Mature_187_residues
MRFELGWCEGARRCESPHFNQRPLGEVSLLVIHNISLPAGCFGLPHIDQLFQGCLDTNADASFAQLQGLEVSAHFLIRRN
GERVQYVSCNDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYTPEQYQSLTALTQALLAEYPSLSTERIVGHCDIAPM
RKTDPGQSFDWERYLGKLKEYTQALRS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=175, Percent_Identity=60.5714285714286, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1787092, Length=145, Percent_Identity=37.2413793103448, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21090; Mature: 21090

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFELGWCEGARRCESPHFNQRPLGEVSLLVIHNISLPAGCFGLPHIDQLFQGCLDTNAD
CCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
ASFAQLQGLEVSAHFLIRRNGERVQYVSCNDRAWHAGVSCYGERENCNDFSIGIELEGTD
CCHHHHCCCCEEEEEEEEECCCEEEEEECCCCCEECCCEEECCCCCCCEEEEEEEEECCC
TEPYTPEQYQSLTALTQALLAEYPSLSTERIVGHCDIAPMRKTDPGQSFDWERYLGKLKE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
YTQALRS
HHHHHCC
>Mature Secondary Structure
MRFELGWCEGARRCESPHFNQRPLGEVSLLVIHNISLPAGCFGLPHIDQLFQGCLDTNAD
CCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCC
ASFAQLQGLEVSAHFLIRRNGERVQYVSCNDRAWHAGVSCYGERENCNDFSIGIELEGTD
CCHHHHCCCCEEEEEEEEECCCEEEEEECCCCCEECCCEEECCCCCCCEEEEEEEEECCC
TEPYTPEQYQSLTALTQALLAEYPSLSTERIVGHCDIAPMRKTDPGQSFDWERYLGKLKE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
YTQALRS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]