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The map label for this gene is ampE [H]

Identifier: 126176105

GI number: 126176105

Start: 4609285

End: 4610136

Strand: Reverse

Name: ampE [H]

Synonym: Sbal_3915

Alternate gene names: 126176105

Gene position: 4610136-4609285 (Counterclockwise)

Preceding gene: 126176106

Following gene: 126176104

Centisome position: 89.91

GC content: 45.66

Gene sequence:

>852_bases
ATGGCATTATTTTCACTCTTGGCCGCCATTTTAGTGGAGCGACTTAAATTTCTTCCTTCATCATGGCAATTTGATCGTTT
ACTGCAATCCTACCAAACGACGTTTTTTGGTGATAAAGCCTCACTGACGAGCGTTATGATGGCGCTGGCGTTAGTGCTGC
CAGCCCTGTTGGTATATGTATTGTCTTGGTTAGTGGCGGGGATGTTTTGGGGTTTATTAAGTTTAGCGCTATGGATTGTC
GTGGCGGTGGTTTGTTTTAACCATCAGAAGCAGCGTGATATTTTCAAGAAATATATGCAAGCGGCTTGTCGCTCCGATGT
GCAAGCTTGTTACCATTATGCCGCTGAGCTCGATTGTAGCGAGTGTTTGGATGCGGTATCCGAGAAAGACCTTGGCGCTA
AAGTGGGTCAAAGTGTTGCTTGGATTAATTATCGTTATTACGGCGCCGTTGCTTTGTTCTTCATTTTTCTGGGGCCAGTC
GGTGCTGTGTTGTATTGCACAGTGCGTTTTTATGCAGAAGAAAATGCCCGTAAATCCCTTGAGCTGCCATTAGTCAACGA
AGTGATTAGCGTGTTGGATTGGCTACCTAGCCGCATCTTTGCCTTTGGTTATGCGTTAAGTGGCCAATTTAGCGAAGGGT
TAGCCGCATGGAGCCATCATGGTTTGACCATAAATACCAGTGCTCGCACTGTCGTTGTTGAGACGGCATTAGCAGCGCAA
CCTTTACCTGAGGTCGCAAGTGCACCTATATGTGTACAGTCAACCTTGGCATTATTAGTACTGAGTAAACGCAACTTTAC
ACTTATCGTCGCGGTACTGTCACTGCTGACCATTTTTGGATTAGTGAGTTAA

Upstream 100 bases:

>100_bases
AAAGGAATATACTCAGGCGTTGCGCTCTTAGGTTTTTGGATTTTAGACGTATTTCACACAAACCTAGACTATTTATGGAC
AAATTATAGAGGTACAGCCC

Downstream 100 bases:

>100_bases
GCAATATCAGCGTCACAGCGATAGCTGAATTTGGCAGTCTGAGCTGAGATAGTTCAGCGCTGTCAAATGGTCTGACCCCA
TAGACATTTTTTTCCGCTAT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALFSLLAAILVERLKFLPSSWQFDRLLQSYQTTFFGDKASLTSVMMALALVLPALLVYVLSWLVAGMFWGLLSLALWIV
VAVVCFNHQKQRDIFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFFIFLGPV
GAVLYCTVRFYAEENARKSLELPLVNEVISVLDWLPSRIFAFGYALSGQFSEGLAAWSHHGLTINTSARTVVVETALAAQ
PLPEVASAPICVQSTLALLVLSKRNFTLIVAVLSLLTIFGLVS

Sequences:

>Translated_283_residues
MALFSLLAAILVERLKFLPSSWQFDRLLQSYQTTFFGDKASLTSVMMALALVLPALLVYVLSWLVAGMFWGLLSLALWIV
VAVVCFNHQKQRDIFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFFIFLGPV
GAVLYCTVRFYAEENARKSLELPLVNEVISVLDWLPSRIFAFGYALSGQFSEGLAAWSHHGLTINTSARTVVVETALAAQ
PLPEVASAPICVQSTLALLVLSKRNFTLIVAVLSLLTIFGLVS
>Mature_282_residues
ALFSLLAAILVERLKFLPSSWQFDRLLQSYQTTFFGDKASLTSVMMALALVLPALLVYVLSWLVAGMFWGLLSLALWIVV
AVVCFNHQKQRDIFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFFIFLGPVG
AVLYCTVRFYAEENARKSLELPLVNEVISVLDWLPSRIFAFGYALSGQFSEGLAAWSHHGLTINTSARTVVVETALAAQP
LPEVASAPICVQSTLALLVLSKRNFTLIVAVLSLLTIFGLVS

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=294, Percent_Identity=27.5510204081633, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31215; Mature: 31084

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01171 RIBOSOMAL_L21E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFSLLAAILVERLKFLPSSWQFDRLLQSYQTTFFGDKASLTSVMMALALVLPALLVYV
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LSWLVAGMFWGLLSLALWIVVAVVCFNHQKQRDIFKKYMQAACRSDVQACYHYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFFIFLGPVGAVLYCTVRFYAEENARKSL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELPLVNEVISVLDWLPSRIFAFGYALSGQFSEGLAAWSHHGLTINTSARTVVVETALAAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHC
PLPEVASAPICVQSTLALLVLSKRNFTLIVAVLSLLTIFGLVS
CCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFSLLAAILVERLKFLPSSWQFDRLLQSYQTTFFGDKASLTSVMMALALVLPALLVYV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LSWLVAGMFWGLLSLALWIVVAVVCFNHQKQRDIFKKYMQAACRSDVQACYHYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFFIFLGPVGAVLYCTVRFYAEENARKSL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELPLVNEVISVLDWLPSRIFAFGYALSGQFSEGLAAWSHHGLTINTSARTVVVETALAAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHC
PLPEVASAPICVQSTLALLVLSKRNFTLIVAVLSLLTIFGLVS
CCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]