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The map label for this gene is aceF [H]

Identifier: 126176102

GI number: 126176102

Start: 4603541

End: 4605532

Strand: Reverse

Name: aceF [H]

Synonym: Sbal_3912

Alternate gene names: 126176102

Gene position: 4605532-4603541 (Counterclockwise)

Preceding gene: 126176103

Following gene: 126176101

Centisome position: 89.82

GC content: 50.75

Gene sequence:

>1992_bases
ATGGCTGAATTAAAAGAAGTTTTTGTTCCTGATATCGGCGGCGATGAAGTGCAAGTGATCGAAATTTGTGTGGCTGTGGG
TGATACCCTAGCGGCAGAAGAATCGATTCTTACCGTCGAAAGCGACAAGGCGACTATGGATATTCCAGCGCCTTTCGCCG
GTGTTTTAGCCGAATTGAAAGTGGCTGTGGGCGATAAAGTATCTGAAGGTACTTTGATTGCTATGATGCAAGCAGCGGGC
GCGGCAGCTGCTGCTCCGGCTCCAGTTGCAGCACCCGCTCAGGCGGCACCAGCATCTGCACCAGTTCAAGCGGCTCCTGC
CCCAGTGGCTGCTGCGACGGGCGCAACCAAAGTGGTTGAAGTGACTGTACCTGATATCGGTGGCGACACTGATGTGTCGG
TTATCGAAGTCCTGGTTGCTGTTGGTGACAAGATTGAAGTTGATAGCGGTTTAATTACCCTAGAAACAGACAAAGCGACC
ATGGACGTACCATCACCTTTCGCGGGTGTGGTTAAAGACGTTAAAGTCGCAGTTGGCGATAAAGTGTCGCAAGGCTCTTT
AGTCATCATGCTTGAAGTGGGCGGCGCTGCACCTGTAGCCGCGCCAACTGTTGCTGCCCAAGCGGCTCCTGCCGCTACGG
TAACGCCAGTGGCCGCAGCACCTGCTGCACCAGCGGCAAGTGTGGTTGCGGTAAAAGAAATTCAAGTGCCGGATATTGGC
GATGCCAGCAATGTCGATGTGATCGAAGTGCTCGTGTCTGTTGGTGATGAAATCACTGCTGACCAAGGTTTGATTACCCT
TGAAACTGACAAAGCCACCATGGAAGTGCCTGCGCCATTCGCCGGTAAACTGCTGTCTTTGACTGTTAAAGTGGGTGACA
AGGTTTCTCAAGGTAGCGTTATTGCGACGATTGAAACTGTGACTGCAGGTGCAGCTCCTGCGCCTGTTGCTCAAGCGGCA
GCTCCTGCTCCAGTGAGCGCGGCGCCCATTGCAGCCCCAACACCTGCAAGCCGTCCTCCAGTGCCACACCACCCAAGCGC
AGGTACGCCTGTGTCTACCGGTGCGGTACATGCGTCTCCTGCGGTACGTCGTTTAGCCCGTGAATTTGGTGTCGACTTGA
CCCAAGTTGCTGGTACTGGCCGTAAAGGTCGCATCATGAAAGAAGACGTACAGGCGTATGTGAAATACGAACTGTCTCGT
CCGAAAGCGACTGCGGCAACCTCTGTTGGTTCAGGTAACGGCGGCGGTCTGCAAGTGATTGCAGCACCTAAAGTTGATTT
CAGTAAGTTTGGTGAAGTGGAAGAGATTCCATTAAGCCGTATCCAGAAGATTTCAGGTCCTAACTTACACCGCAACTGGG
TAACTATCCCTCACGTGACTCAGTTCGATGAAGCTGATATCACTGAAATGGAAGAGTTCCGTAAGCAGCAAAACGATGTA
GCGGCGAAGAAAAAAGCCGATTACAAGATCACGCCTTTAGTGTTTATGCTAAAAGCTGTGGCTAAAACGTTGCAACAGTT
CCCAGTGTTCAACTCAAGCTTAAGTAGCGATGGCGAATCACTGATCCAGAAGAAGTATTTCCATATCGGTGTGGCGGTAG
ATACGCCAAACGGTTTGGTTGTGCCAGTCGTGCGTGACGTGGATAAGAAAGGCATTATCGAGTTATCTCGTGAACTGGCT
GATATCTCTATCCGTGCCCGTGATGGCAAGCTGAAATCTGCTGACATGCAAGGTAGCTGTTTTACTATTTCAAGTTTAGG
TGGCATTGGCGGTACAGCGTTTACGCCTATCGTTAACTACCCAGACGTGGCGATTTTAGGTGTGTCTAAATCTGAAATTA
AGCCTAAGTGGAATGGTAAAGAGTTCGAACCTAAATTGATGTTGCCATTGTCGCTGTCATACGATCACCGCGTGATCGAT
GGTGCTATGGCTGCACGCTTTAGTGTGACGCTGTCAGGAATTCTGTCCGATATTCGTACTTTGGTTCTGTAA

Upstream 100 bases:

>100_bases
TCGCAAAGAGCTGCCTGTCGATGTGTTAGTAAAAGCCATTAAAGAATATGGTATCGACGCTGACAAGATCAATCCACAGT
ACGCGTAAGAGGCAATAAAA

Downstream 100 bases:

>100_bases
GCATATAAGGCTGCTCATCTTGAGTAGCCTTTTGTTTATTGTGATCAGTATCAAACACAGGTTAAAATGCGCCCACCTTA
CGCGCTGGCGCATTTTCGGT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAAAPAPVAAPAQAAPASAPVQAAPAPVAAATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKAT
MDVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPVAAPTVAAQAAPAATVTPVAAAPAAPAASVVAVKEIQVPDIG
DASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAPVAQAA
APAPVSAAPIAAPTPASRPPVPHHPSAGTPVSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMKEDVQAYVKYELSR
PKATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQNDV
AAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSRELA
DISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVID
GAMAARFSVTLSGILSDIRTLVL

Sequences:

>Translated_663_residues
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAG
AAAAAPAPVAAPAQAAPASAPVQAAPAPVAAATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKAT
MDVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPVAAPTVAAQAAPAATVTPVAAAPAAPAASVVAVKEIQVPDIG
DASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAPVAQAA
APAPVSAAPIAAPTPASRPPVPHHPSAGTPVSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMKEDVQAYVKYELSR
PKATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQNDV
AAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSRELA
DISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVID
GAMAARFSVTLSGILSDIRTLVL
>Mature_662_residues
AELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELKVAVGDKVSEGTLIAMMQAAGA
AAAAPAPVAAPAQAAPASAPVQAAPAPVAAATGATKVVEVTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATM
DVPSPFAGVVKDVKVAVGDKVSQGSLVIMLEVGGAAPVAAPTVAAQAAPAATVTPVAAAPAAPAASVVAVKEIQVPDIGD
ASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSVIATIETVTAGAAPAPVAQAAA
PAPVSAAPIAAPTPASRPPVPHHPSAGTPVSTGAVHASPAVRRLAREFGVDLTQVAGTGRKGRIMKEDVQAYVKYELSRP
KATAATSVGSGNGGGLQVIAAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQNDVA
AKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLVVPVVRDVDKKGIIELSRELAD
ISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNYPDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDG
AMAARFSVTLSGILSDIRTLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=27.8801843317972, Blast_Score=164, Evalue=4e-40,
Organism=Homo sapiens, GI31711992, Length=328, Percent_Identity=33.2317073170732, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=444, Percent_Identity=27.7027027027027, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=27.9279279279279, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.3612334801762, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=36.0544217687075, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786305, Length=659, Percent_Identity=55.8421851289833, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=30.0733496332518, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=29.1764705882353, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=30.9468822170901, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=214, Percent_Identity=38.785046728972, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=30.4487179487179, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=453, Percent_Identity=29.1390728476821, Blast_Score=143, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=25.5813953488372, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=29.0023201856148, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=35.5140186915888, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582497, Length=316, Percent_Identity=28.1645569620253, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68037; Mature: 67906

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCCHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAAAPAPVAAPAQAAPASAPVQAAPAPVAAATGATKVVE
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEE
VTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKDVKVAVGD
EEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KVSQGSLVIMLEVGGAAPVAAPTVAAQAAPAATVTPVAAAPAAPAASVVAVKEIQVPDIG
CCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCC
DASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSV
CCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCCCE
IATIETVTAGAAPAPVAQAAAPAPVSAAPIAAPTPASRPPVPHHPSAGTPVSTGAVHASP
EEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCH
AVRRLAREFGVDLTQVAGTGRKGRIMKEDVQAYVKYELSRPKATAATSVGSGNGGGLQVI
HHHHHHHHHCCCEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEE
AAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQNDV
EECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLV
HHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCCEE
VPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNY
EEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGILSDIRT
CCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVL
HCC
>Mature Secondary Structure 
AELKEVFVPDIGGDEVQVIEICVAVGDTLAAEESILTVESDKATMDIPAPFAGVLAELK
CCCHHHHCCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEECCCCEEECCCCHHHHHHHHH
VAVGDKVSEGTLIAMMQAAGAAAAAPAPVAAPAQAAPASAPVQAAPAPVAAATGATKVVE
HHHCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEE
VTVPDIGGDTDVSVIEVLVAVGDKIEVDSGLITLETDKATMDVPSPFAGVVKDVKVAVGD
EEECCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KVSQGSLVIMLEVGGAAPVAAPTVAAQAAPAATVTPVAAAPAAPAASVVAVKEIQVPDIG
CCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCC
DASNVDVIEVLVSVGDEITADQGLITLETDKATMEVPAPFAGKLLSLTVKVGDKVSQGSV
CCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEEEEEEECCCCCCCCE
IATIETVTAGAAPAPVAQAAAPAPVSAAPIAAPTPASRPPVPHHPSAGTPVSTGAVHASP
EEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCH
AVRRLAREFGVDLTQVAGTGRKGRIMKEDVQAYVKYELSRPKATAATSVGSGNGGGLQVI
HHHHHHHHHCCCEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEE
AAPKVDFSKFGEVEEIPLSRIQKISGPNLHRNWVTIPHVTQFDEADITEMEEFRKQQNDV
EECCCCHHHCCCCHHCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AAKKKADYKITPLVFMLKAVAKTLQQFPVFNSSLSSDGESLIQKKYFHIGVAVDTPNGLV
HHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCCEE
VPVVRDVDKKGIIELSRELADISIRARDGKLKSADMQGSCFTISSLGGIGGTAFTPIVNY
EEEECCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PDVAILGVSKSEIKPKWNGKEFEPKLMLPLSLSYDHRVIDGAMAARFSVTLSGILSDIRT
CCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]