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The map label for this gene is lpdA [H]

Identifier: 126176101

GI number: 126176101

Start: 4601966

End: 4603393

Strand: Reverse

Name: lpdA [H]

Synonym: Sbal_3911

Alternate gene names: 126176101

Gene position: 4603393-4601966 (Counterclockwise)

Preceding gene: 126176102

Following gene: 126176100

Centisome position: 89.78

GC content: 46.43

Gene sequence:

>1428_bases
ATGAGTAACGAAATCAAAACTCAGGTAGTGGTATTAGGTGCAGGTCCTGCAGGTTATTCTGCGGCTTTCCGTGCAGCGGA
CTTAGGTCTTGAAACCATTATCGTTGAACGTTTTAGCACTTTAGGCGGCGTGTGTCTTAACGTGGGTTGTATCCCATCTA
AAGCCCTATTACATGTTGCTAAAGTTATCGAAGAAGCCAAAGCCGTTGCTGCTCACGGTGTGGTTTTCGGCGAGCCAACT
ATCGATTTAGATAAGTTACGTGGCTTTAAAGACAAAGTCATTGGCCAATTAACTGGCGGATTGGGCGGTATGTCCAAAAT
GCGTAAAGTTAACGTGGTTAACGGTTTTGGTAAATTTACTGGCCCTAACACGCTAGAAGTGACTGCTGAAGATGGCACTG
TCAAAGTGGTTCAATTCGAGCAAGCTATCATTGCTGCGGGTTCTCGCCCAATCAAACTGCCATTCATTCCGCATGAAGAT
CCACGTATTTGGGATTCGACTGACGCATTAGAACTGAAAGAAGTTCCAGGCAAGTTGTTAGTGATGGGCGGCGGTATTAT
CGGCCTAGAAATGGGTACTGTGTACGCTTCTTTAGGCAGTGAAATCGACGTGGTTGAAATGTTCGACCAAGTGATCCCAG
CAGCTGATAAAGACGTTGTTCGCGTATTCACTAAGCAAATCAAGAAGAAATTCAACCTGATCCTTGAAACTAAAGTCACA
GCGGTAGAAGCCCGTGAAGACGGTATCTATGTTTCTATGGAAGGTAAGAGCGCACCAACTGAAGCTGTCCGTTACGATGC
AGTCTTAGTAGCAATTGGCCGTGCACCTAATGGCAAGTCGTTAGATGCTGAAAAAGCAGGTGTTAACGTTGATGAGCGTG
GCTTTATCAAGGTTGATAAGCAACTGCGTACTAACGTACCGCACATCTATGCAATTGGTGACATCGTTGGTCAACCTATG
TTGGCTCACAAAGGCGTGCACGAAGGCCACGTAGCGGCTGAAGTTATCGCTGGTATGAAGCACTACTTCGATCCAAAAGT
GATCCCATCAATTGCTTACACTGACCCAGAAGTCGCTTGGGTTGGTTTAACTGAGAAAGAAGCGAAAGAGCAAGGTATTG
CCTACGAAACAGCTACTTTCCCATGGGCAGCAAGCGGCCGCGCTATCGCATCTGATGCGAGCGAAGGCATGACTAAACTG
ATTTTCGACAAAGACACTCATCGCGTAATCGGTGGTGCGATTGTTGGCGTTAACGGCGGCGAGCTGTTAGGTGAAATCGG
TTTAGCGATTGAAATGGGTTGTGATGCTGAAGATTTAGCATTAACCATTCACGCGCACCCAACACTGCACGAATCAGTGG
GCTTAGCGGCTGAAATGTACGAAGGTTCAATTACTGATTTGCCAAACCCAAAGGCAAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
GTATCAAACACAGGTTAAAATGCGCCCACCTTACGCGCTGGCGCATTTTCGGTTGGCAGGATAGCTCCTCCAACGGATTG
AATGAGAATTAGAGGAAAAC

Downstream 100 bases:

>100_bases
TTTAGCTGTCGATTAAAAAAGCGCTCATTTGAGCGCTTTTTTATTGCCTCTATTTTTTTGTAACTTTTATGTAAACAAGC
GGATGAATTGATAGGATTGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRGFKDKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPT
IDLDKLRGFKDKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHED
PRIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPM
LAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKL
IFDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVAKVIEEAKAVAAHGVVFGEPTI
DLDKLRGFKDKVIGQLTGGLGGMSKMRKVNVVNGFGKFTGPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVTA
VEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDKQLRTNVPHIYAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLI
FDKDTHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.7312775330396, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=27.9120879120879, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI148277071, Length=429, Percent_Identity=30.0699300699301, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=30.0699300699301, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=30.0699300699301, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=30.0699300699301, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=28.9351851851852, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.6026200873362, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=27.4647887323944, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=85.4736842105263, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=27.5641025641026, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=28.7280701754386, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=28.6681715575621, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=40.3083700440529, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.6919831223629, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=27.4774774774775, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=27.4774774774775, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=451, Percent_Identity=26.8292682926829, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.8859649122807, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.1367521367521, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=27.3127753303965, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=41.1378555798687, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=29.4243070362473, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=29.4243070362473, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=29.4243070362473, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=27.2151898734177, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50451; Mature: 50320

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRGFKDKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCEEEEEECCCEEEEEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDK
EEEECCCCEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEECCCHHCCCEEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETIIVERFSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVAAHGVVFGEPTIDLDKLRGFKDKVIGQLTGGLGGMSKMRKVNVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHEEEEEECCCCCC
GPNTLEVTAEDGTVKVVQFEQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
CCCEEEEEECCCEEEEEEEEEHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEE
VMGGGIIGLEMGTVYASLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
EEECCEEEEEHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAREDGIYVSMEGKSAPTEAVRYDAVLVAIGRAPNGKSLDAEKAGVNVDERGFIKVDK
EEEECCCCEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECH
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEQGIAYETATFPWAASGRAIASDASEGMTKLIFDKDTHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEECCCHHCCCEEEEECCCCCEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEMYEGSITDLPNPKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]