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The map label for this gene is metB [H]

Identifier: 126175969

GI number: 126175969

Start: 4434079

End: 4435242

Strand: Reverse

Name: metB [H]

Synonym: Sbal_3776

Alternate gene names: 126175969

Gene position: 4435242-4434079 (Counterclockwise)

Preceding gene: 126175972

Following gene: 126175968

Centisome position: 86.5

GC content: 51.03

Gene sequence:

>1164_bases
ATGGCAGAGCGTCAATTAGCCACACTGGCAGTGCGTCAGGGTATCGAAAGCGATACTCAATATGGTGCAGTTGTGCCACC
GATTTATTTGTCGACTAATTACGCCTTCGATGGTCATAAAAATCCCCGCGAATTCGATTACAGTCGTTCAGGCAACCCAA
CGCGCTGCATTTTAGGTGAAGCATTAGCCCAATTAGAAAAGGGCGCCACTGGCATAGTGACATGCACTGGCATGGCGGCC
ATCACCCTAGTGACCACATTGCTCGGCCCTGATGACCTGTTGGTGGTGCCCCATGATTGTTACGGCGGTTCTTACCGTTT
ATTTACTAACTTGGCGAAGAAAGGCCAGTTTAAGTTGATTGTGGTCGATCAAACCGACAGCCAAGCGTTAGCCGCCGCTA
TCGGGCAACATCCTAAGATGGTGTGGCTCGAAACCCCATCTAACCCGCTATTGCGTGTGGTGGATATCGAAGCCATTGCT
AAGGCGAGCCATGAGGTCGGCGCGCTGGTGGTGGTCGATAACACTTTTTTATCGCCGATATTGCAACAACCCCTATTGTT
AGGCGCAGATATCGTTATCCACTCCACCACTAAATATATCAATGGTCACAGCGATGTGGTTGGCGGCGCAGTGGTTGCTA
AAGATGCACAAATTGGCGAAAGCTTACATTGGTGGTCGAACACTTTAGGCTTAACGGGATCGGCTTTCGACAGCTACTTA
ACCCTGCGCGGCCTACGTACCTTAGCGGTGCGAATTCGTGAACATCAAACCAATGCCCAGCACATTGTTGAGGTATTGAC
GCAAAGCCCTGTGGTGAGCAAAGTTTATTACCCTGGTCTTGCGGATCACCCAGGTCATGCCATTGCTGCCAAGCAACAAA
AAGGCTTTGGCGCTATGCTGAGTTTTGAACTCAAGGGCGGCGAGGCCGAAGTGGTGGCCTTTTTAGGTGCACTCAGCTTA
TTTAGTGTGGCGGAAAGTTTAGGTGGCGTTGAAAGTTTGGTGGCCGTGCCCGCAACTATGACCCACAGAGCCATGGAGCC
GCAAGCGCGCTTCGAGGCAGGTATTAAGGACACCCTATTGCGTTTATCGGTGGGTATTGAAGATGCCGCCGATTTAGTTG
CAGATATTCAAGCAGGATTAGCCGCCGTTGCGGCTTGTCAGTAG

Upstream 100 bases:

>100_bases
ACGTCTAAACGTCTTCACGCCTATTGACGATGGCGTTGGGCTTAGGTACATTTAGACGTCCAGACATCTCTATCAGTCTA
CCGAATTTAGGAGTCCACCC

Downstream 100 bases:

>100_bases
AGGAATAATAGATGGCACGTTGTCACTTACATAAATTTGGTGGCTCCAGTTTAGCGGATGCCGATTGTTATCGCCGAGTC
GCCCATATCCTGTTAACCCA

Product: O-succinylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAERQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRCILGEALAQLEKGATGIVTCTGMAA
ITLVTTLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLIVVDQTDSQALAAAIGQHPKMVWLETPSNPLLRVVDIEAIA
KASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDAQIGESLHWWSNTLGLTGSAFDSYL
TLRGLRTLAVRIREHQTNAQHIVEVLTQSPVVSKVYYPGLADHPGHAIAAKQQKGFGAMLSFELKGGEAEVVAFLGALSL
FSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLLRLSVGIEDAADLVADIQAGLAAVAACQ

Sequences:

>Translated_387_residues
MAERQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRCILGEALAQLEKGATGIVTCTGMAA
ITLVTTLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLIVVDQTDSQALAAAIGQHPKMVWLETPSNPLLRVVDIEAIA
KASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDAQIGESLHWWSNTLGLTGSAFDSYL
TLRGLRTLAVRIREHQTNAQHIVEVLTQSPVVSKVYYPGLADHPGHAIAAKQQKGFGAMLSFELKGGEAEVVAFLGALSL
FSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLLRLSVGIEDAADLVADIQAGLAAVAACQ
>Mature_386_residues
AERQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRCILGEALAQLEKGATGIVTCTGMAAI
TLVTTLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLIVVDQTDSQALAAAIGQHPKMVWLETPSNPLLRVVDIEAIAK
ASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDAQIGESLHWWSNTLGLTGSAFDSYLT
LRGLRTLAVRIREHQTNAQHIVEVLTQSPVVSKVYYPGLADHPGHAIAAKQQKGFGAMLSFELKGGEAEVVAFLGALSLF
SVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLLRLSVGIEDAADLVADIQAGLAAVAACQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=43.1318681318681, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI299473759, Length=364, Percent_Identity=40.9340659340659, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI25453487, Length=363, Percent_Identity=36.9146005509642, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=61.3157894736842, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=30.7304785894207, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=367, Percent_Identity=44.6866485013624, Blast_Score=304, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI17538055, Length=367, Percent_Identity=44.4141689373297, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=33.2432432432432, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319307, Length=384, Percent_Identity=41.1458333333333, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=33.5802469135802, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=32.1184510250569, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=402, Percent_Identity=29.3532338308458, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=26.3157894736842, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322970, Length=277, Percent_Identity=25.2707581227437, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323557, Length=260, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322590, Length=282, Percent_Identity=25.886524822695, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=42.3280423280423, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41116; Mature: 40984

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRCILGE
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECEEECCCCCCCCCCCCCCCCCCCHHHHH
ALAQLEKGATGIVTCTGMAAITLVTTLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLI
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALAAAIGQHPKMVWLETPSNPLLRVVDIEAIAKASHEVGALVVVDNTFLSPI
EEECCCCHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDAQIGESLHWWSNTLGLTGSAFDSYL
HHCCHHHCCEEEEECCHHHCCCCCCCCCCEEEECCHHHCCHHHHHHHCCCCCCHHHHHHH
TLRGLRTLAVRIREHQTNAQHIVEVLTQSPVVSKVYYPGLADHPGHAIAAKQQKGFGAML
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEECCCCCCCCCCEEEECCCCCCCEEE
SFELKGGEAEVVAFLGALSLFSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLL
EEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCHHHHHCCHHHHHH
RLSVGIEDAADLVADIQAGLAAVAACQ
HHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AERQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRCILGE
CCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECEEECCCCCCCCCCCCCCCCCCCHHHHH
ALAQLEKGATGIVTCTGMAAITLVTTLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLI
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
VVDQTDSQALAAAIGQHPKMVWLETPSNPLLRVVDIEAIAKASHEVGALVVVDNTFLSPI
EEECCCCHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVVAKDAQIGESLHWWSNTLGLTGSAFDSYL
HHCCHHHCCEEEEECCHHHCCCCCCCCCCEEEECCHHHCCHHHHHHHCCCCCCHHHHHHH
TLRGLRTLAVRIREHQTNAQHIVEVLTQSPVVSKVYYPGLADHPGHAIAAKQQKGFGAML
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEECCCCCCCCCCEEEECCCCCCCEEE
SFELKGGEAEVVAFLGALSLFSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLL
EEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCHHHHHCCHHHHHH
RLSVGIEDAADLVADIQAGLAAVAACQ
HHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]