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The map label for this gene is metL [H]

Identifier: 126175968

GI number: 126175968

Start: 4431674

End: 4434067

Strand: Reverse

Name: metL [H]

Synonym: Sbal_3775

Alternate gene names: 126175968

Gene position: 4434067-4431674 (Counterclockwise)

Preceding gene: 126175969

Following gene: 126175967

Centisome position: 86.48

GC content: 51.71

Gene sequence:

>2394_bases
ATGGCACGTTGTCACTTACATAAATTTGGTGGCTCCAGTTTAGCGGATGCCGATTGTTATCGCCGAGTCGCCCATATCCT
GTTAACCCACGGTCATAGCGATGACCTTGTGGTGGTCTCTGCGGCGGGTAAGAGCACGAATTTCCTGTATAAATTATTAG
CGCTGCGCGACGTGGGTCAGTTGTGGCAAGAAGAATTACAAGTGCTGACCAGCTATCAACAGGGGTTGGTAGAGCAGTTA
TTGTCGAACGAACAAGCCCGCGATCTGCGTGAGCGTTTGGCGACAGATCAGGCGCAGCTCATCAGTTTATTGTCCCTCGA
TGAACGCAACGAATACCAAGTTAACCATGTGGTGAGCTTTGGCGAGCGTTGGTCGGCGCGCTTGATGGCGGCGCTGCTGC
GCGAATCTGGTGTGGCGGCAAGCCATGTGGATGCCTGTTCAATTTTAGTGGCCGATGAAGCCGCCGTGCCACAAATTCGG
GTGCAGGAATCCCGCGCAAAAGTACAAGCGCTGTTGGCCGCCCACCCAAATGAACGACTAGTGATCACAGGCTTTATCTG
CGCTAACGAGCGTGGCGATACTTTATTACTTGGTCGTAATGGTTCCGATTTTAGCGCTAGCTTGATTGCCAGCCTTGCCG
ATATTGAGCGCGTGACCATTTGGACCGATGTGGAAGGGGTGTTCAACGCTGACCCGAATAAAATCAACGATGCTAAATTA
CTAAAAAGCATGTCACTTGCCGAAGCTGACCGCTTAGCGCGTTTAGGCTCGCCGGTATTGCATTCGCGCACTTTGCAGCC
TCTGTTTAATACCGAAGTGAGTTTAGCGGTGCGCTCAAGTTATGCATCGCACACCGACTTTACCTTGATTGCGCCCCACA
GTTCGTCGGCGAGCGCGCCTGTGGTGACTAACCTCAATGCTGTTGTCCTGTTCGGCTTTAAGCTCACGGGCGAATTAACA
AGCTTACTAGATTCGTTGATCGCCGCAGGTTTAACGCCATTGGCCCATTGGACCTCAGCCCATCAAAGGGTTGAGCTTGC
CTATACCGCTGAAAATCAAAAACAAGTACAAAGATTACTCGACGCCGAGGCACAAGTATTAGGGATCAGCGATCAGCAGA
TCAACACAGATCTCGGCCTAGTGGCGTTAGTCAGTGCCGATGCCGAACATTATCGCCGCAGCTTCGCCCGTTTATTAAGT
CGTGACGCTAAACCTTTGTTTAGTGGCGACTTAAGCTTAGTGACGCTGGTGCCTCAGTCGCAGGTGAATCTGTTAACGCA
AAAGGTCCACCGTCGCTGCGCTGGGCCGCGTAAACGCATTGGTGTCTTACTGCTCGGCGTGGGCAACATAGGTGAGGCTT
GGGTCGATTTGTTTAAGTCGGTGTCGCCATCACTGGATCATGAACTCGAAGCTAGCGTTGAGTTAGTCGGCTTAGTCAGT
TCGAGTAAAGCCTTGATTAAATCCACAGGCATTAATCTTGCCGCGTGGCAGACTGAGTTTAATACCGAGGCAACACCTTG
GCAGTACGAGCACTTGTTCGAGCAATTAGAACGGGTTAACTGCGATGAGCTGGTGGCCTTAGATATCAGTGCCAGCGCCA
GTTTGACGCTGCAATATCCAGAGCTATTCGAGCGCGGCATCCATGTGGTGAGCGCGAATAAACTCGCAGGCTCGGGTCCA
CTGCCTTTTTACCGTGAGTTAAAGCAGCAACTGGGTAATCGTCGTTTATTTTGGCGCTATAACGCCAGTTGCGGCGCAGG
TTTGCCTATCCAACATGCGTTAAATGATCTGCGTAACAGCGGTGATAGCGTTGAAGCCGTGGGCGGGATTTTCTCTGGCA
CCTTGTGTTGGTTGTTTGAGAAATACGATACCAGCAAGCCTTTCTCTGAGTTAGTGATTGAAGCCAGAGGCTTAGGTATC
ACTGAGCCCGATCCCCGCGACGATCTGTCCGGTCGTGATATGCAGCGTAAACTGCTCATTTTGGCCCGTGAAATTGGCCT
TGAAATCGAGCTTGAAGATATTGAACTGCGCTCATTAGTGCCAGCCGATTTGGCCGATATTCCCTTAGATCAATTTCTTG
CCCGCATTGCCGAGTTAGATGCCGATCTGCTGCAACAATACGGTGCGGCGGCGGAGCAAAATAAGGTGCTCAGATACGTG
GCATCCTTAGATAACAGCGGCCCAGAGCTTAAAGCTGAAGTCGGTTTGCAATGGATTGATGCCCATCATCCCTACGCTAA
TTTGACTCCAGGCGATAACGTGTTTGTGATCCGCAGCGTCTTCTATCAAGGTAACCCGTTGATCATCCGTGGACCGGGCG
CCGGACGCGAAGTGACAGCGGCGGCCGTGCAGTCGGATCTGACGCAGATCTGTCGCGATCTCTTGCAAGAGTAG

Upstream 100 bases:

>100_bases
TATTGCGTTTATCGGTGGGTATTGAAGATGCCGCCGATTTAGTTGCAGATATTCAAGCAGGATTAGCCGCCGTTGCGGCT
TGTCAGTAGAGGAATAATAG

Downstream 100 bases:

>100_bases
ACTGAAATTCAGCAGCTTAAAGGACTCATTCGAGTCCTTTTTTATTGGCTAGAATAAATCTATCGAATTAACCGAGATTA
AATGCGGTTTATGGACATTT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLALRDVGQLWQEELQVLTSYQQGLVEQL
LSNEQARDLRERLATDQAQLISLLSLDERNEYQVNHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEAAVPQIR
VQESRAKVQALLAAHPNERLVITGFICANERGDTLLLGRNGSDFSASLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTEVSLAVRSSYASHTDFTLIAPHSSSASAPVVTNLNAVVLFGFKLTGELT
SLLDSLIAAGLTPLAHWTSAHQRVELAYTAENQKQVQRLLDAEAQVLGISDQQINTDLGLVALVSADAEHYRRSFARLLS
RDAKPLFSGDLSLVTLVPQSQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVDLFKSVSPSLDHELEASVELVGLVS
SSKALIKSTGINLAAWQTEFNTEATPWQYEHLFEQLERVNCDELVALDISASASLTLQYPELFERGIHVVSANKLAGSGP
LPFYRELKQQLGNRRLFWRYNASCGAGLPIQHALNDLRNSGDSVEAVGGIFSGTLCWLFEKYDTSKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGLEIELEDIELRSLVPADLADIPLDQFLARIAELDADLLQQYGAAAEQNKVLRYV
ASLDNSGPELKAEVGLQWIDAHHPYANLTPGDNVFVIRSVFYQGNPLIIRGPGAGREVTAAAVQSDLTQICRDLLQE

Sequences:

>Translated_797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLALRDVGQLWQEELQVLTSYQQGLVEQL
LSNEQARDLRERLATDQAQLISLLSLDERNEYQVNHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEAAVPQIR
VQESRAKVQALLAAHPNERLVITGFICANERGDTLLLGRNGSDFSASLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTEVSLAVRSSYASHTDFTLIAPHSSSASAPVVTNLNAVVLFGFKLTGELT
SLLDSLIAAGLTPLAHWTSAHQRVELAYTAENQKQVQRLLDAEAQVLGISDQQINTDLGLVALVSADAEHYRRSFARLLS
RDAKPLFSGDLSLVTLVPQSQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVDLFKSVSPSLDHELEASVELVGLVS
SSKALIKSTGINLAAWQTEFNTEATPWQYEHLFEQLERVNCDELVALDISASASLTLQYPELFERGIHVVSANKLAGSGP
LPFYRELKQQLGNRRLFWRYNASCGAGLPIQHALNDLRNSGDSVEAVGGIFSGTLCWLFEKYDTSKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGLEIELEDIELRSLVPADLADIPLDQFLARIAELDADLLQQYGAAAEQNKVLRYV
ASLDNSGPELKAEVGLQWIDAHHPYANLTPGDNVFVIRSVFYQGNPLIIRGPGAGREVTAAAVQSDLTQICRDLLQE
>Mature_796_residues
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLALRDVGQLWQEELQVLTSYQQGLVEQLL
SNEQARDLRERLATDQAQLISLLSLDERNEYQVNHVVSFGERWSARLMAALLRESGVAASHVDACSILVADEAAVPQIRV
QESRAKVQALLAAHPNERLVITGFICANERGDTLLLGRNGSDFSASLIASLADIERVTIWTDVEGVFNADPNKINDAKLL
KSMSLAEADRLARLGSPVLHSRTLQPLFNTEVSLAVRSSYASHTDFTLIAPHSSSASAPVVTNLNAVVLFGFKLTGELTS
LLDSLIAAGLTPLAHWTSAHQRVELAYTAENQKQVQRLLDAEAQVLGISDQQINTDLGLVALVSADAEHYRRSFARLLSR
DAKPLFSGDLSLVTLVPQSQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVDLFKSVSPSLDHELEASVELVGLVSS
SKALIKSTGINLAAWQTEFNTEATPWQYEHLFEQLERVNCDELVALDISASASLTLQYPELFERGIHVVSANKLAGSGPL
PFYRELKQQLGNRRLFWRYNASCGAGLPIQHALNDLRNSGDSVEAVGGIFSGTLCWLFEKYDTSKPFSELVIEARGLGIT
EPDPRDDLSGRDMQRKLLILAREIGLEIELEDIELRSLVPADLADIPLDQFLARIAELDADLLQQYGAAAEQNKVLRYVA
SLDNSGPELKAEVGLQWIDAHHPYANLTPGDNVFVIRSVFYQGNPLIIRGPGAGREVTAAAVQSDLTQICRDLLQE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=805, Percent_Identity=41.9875776397516, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1786183, Length=311, Percent_Identity=38.2636655948553, Blast_Score=215, Evalue=1e-56,
Organism=Escherichia coli, GI1790455, Length=293, Percent_Identity=32.7645051194539, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322599, Length=345, Percent_Identity=32.463768115942, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320893, Length=308, Percent_Identity=26.2987012987013, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 87374; Mature: 87243

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLALRDVGQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQVLTSYQQGLVEQLLSNEQARDLRERLATDQAQLISLLSLDERNEYQVNHVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHHH
GERWSARLMAALLRESGVAASHVDACSILVADEAAVPQIRVQESRAKVQALLAAHPNERL
HHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEE
VITGFICANERGDTLLLGRNGSDFSASLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTEVSLAVRSSYASHTDFTLIAPHSSSASAP
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCEEEEEHHCCCCCCCEEEEECCCCCCCCC
VVTNLNAVVLFGFKLTGELTSLLDSLIAAGLTPLAHWTSAHQRVELAYTAENQKQVQRLL
EEECCCEEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCHHHHHHHH
DAEAQVLGISDQQINTDLGLVALVSADAEHYRRSFARLLSRDAKPLFSGDLSLVTLVPQS
CCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCH
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVDLFKSVSPSLDHELEASVELVGLVS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCEEEEEEEC
SSKALIKSTGINLAAWQTEFNTEATPWQYEHLFEQLERVNCDELVALDISASASLTLQYP
CCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECH
ELFERGIHVVSANKLAGSGPLPFYRELKQQLGNRRLFWRYNASCGAGLPIQHALNDLRNS
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSVEAVGGIFSGTLCWLFEKYDTSKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGLEIELEDIELRSLVPADLADIPLDQFLARIAELDADLLQQYGAAAEQNKVLRYV
HHHHHCCEEEEECCHHHHCCCCCHHCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
ASLDNSGPELKAEVGLQWIDAHHPYANLTPGDNVFVIRSVFYQGNPLIIRGPGAGREVTA
HHHCCCCCCEEHHHCCEEEECCCCCCCCCCCCCEEEEEEEHCCCCEEEEECCCCCCHHHH
AAVQSDLTQICRDLLQE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLALRDVGQ
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQVLTSYQQGLVEQLLSNEQARDLRERLATDQAQLISLLSLDERNEYQVNHVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHHH
GERWSARLMAALLRESGVAASHVDACSILVADEAAVPQIRVQESRAKVQALLAAHPNERL
HHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEE
VITGFICANERGDTLLLGRNGSDFSASLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LKSMSLAEADRLARLGSPVLHSRTLQPLFNTEVSLAVRSSYASHTDFTLIAPHSSSASAP
HHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCEEEEEHHCCCCCCCEEEEECCCCCCCCC
VVTNLNAVVLFGFKLTGELTSLLDSLIAAGLTPLAHWTSAHQRVELAYTAENQKQVQRLL
EEECCCEEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCHHHHHHHH
DAEAQVLGISDQQINTDLGLVALVSADAEHYRRSFARLLSRDAKPLFSGDLSLVTLVPQS
CCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCH
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVDLFKSVSPSLDHELEASVELVGLVS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCEEEEEEEC
SSKALIKSTGINLAAWQTEFNTEATPWQYEHLFEQLERVNCDELVALDISASASLTLQYP
CCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECH
ELFERGIHVVSANKLAGSGPLPFYRELKQQLGNRRLFWRYNASCGAGLPIQHALNDLRNS
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSVEAVGGIFSGTLCWLFEKYDTSKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGLEIELEDIELRSLVPADLADIPLDQFLARIAELDADLLQQYGAAAEQNKVLRYV
HHHHHCCEEEEECCHHHHCCCCCHHCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHH
ASLDNSGPELKAEVGLQWIDAHHPYANLTPGDNVFVIRSVFYQGNPLIIRGPGAGREVTA
HHHCCCCCCEEHHHCCEEEECCCCCCCCCCCCCEEEEEEEHCCCCEEEEECCCCCCHHHH
AAVQSDLTQICRDLLQE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]