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The map label for this gene is pleD [H]

Identifier: 126175898

GI number: 126175898

Start: 4326918

End: 4328300

Strand: Direct

Name: pleD [H]

Synonym: Sbal_3704

Alternate gene names: 126175898

Gene position: 4326918-4328300 (Clockwise)

Preceding gene: 126175896

Following gene: 126175901

Centisome position: 84.39

GC content: 47.51

Gene sequence:

>1383_bases
GTGCCAGACATTTATCCTTTTTTATCGCAGCGCCCCCATAAACATGCGGCGGTGATTGTCGCCCTCTTACTGCTAACATC
AGCTCTTGTGATCTCTCCTTTTATTTCAATACCTTTACCTGATATTGCCCCATTTTTACCTGTGTACGGCACCGCAGTAG
TACTACTCGAAAGTATTACCGCGTATTTATTGCTGTCGCAGTTTGCCAGTAATAGGCAAGTTTTCGCAGGTTTTGTCGCC
AGTGCGTATTTGTTTCTTATCCCCTTAGTCACTGTTCAGCTTTTGGTTTTTCCTGGAGTCTTTGCCCCCAAGGGGTTACT
CAATGCCGGAGCACAAAGTGCAGTTTGGATCTGGGTCTTCTGGCATGCATCGTTTCCAACCTTAATCCTGCTGGCTTTGA
TGGCTCAACAGAAGTTAACAACACAGCAAGTTGCGCCGATGCAACTGAAATATTGGCTGTGGGGTTTTGTATTAATTCCT
GTGTTGTGCAGTTTAGCACTGGCTTTGTTTGCAACATGGGGTAGCCATTGGTTACCAATCCTGATCGCTAAAAATAGCTA
TAAACAGTTATTACACAGCCCTTATGCCTTACTCGTATGGGGACTAAACGCACTGGCTTTAGTCTGCATGATACGGCGCG
CCCGCAGTAACAATGTGCTCTATACATGGCTGAGTGTGGCGCTGCTGGCCAGCCTAATTGATGTCACGTTAACCTTATTC
GCTGGCGCCCGCTTTAGTCTAGGTTGGTACGCCGCAAGGATTAGCAGTTCGGTCTCCTCTATGGTCTTACTCTGCGCCTT
ACTATGGGAAGTCAATAGGCTGTATGTCGCAACCCAAAGGGCGAATGAGTCGCTGTACCAGCAGAGTGTCAAAGACACCT
TAACAGGCTTATTTAACCGCCGTTATCTAGAATCTCAGCTGGCAATACACATAGACCATGCGAAAAGGCATAAAGAGCCA
CTGTGCTTGTTAGTGCTCGATGTCGATCACTTTAAAGCCTTTAACGACCAGCATGGCCATTTGGCTGGGGACGACTGCCT
AGTGGCCGTCGCAAAACAACTGGAGCAGAGTTTATATAGACCAGCGGATTTCATCGCCCGTTATGGTGGTGAAGAGTTTG
TCGTAGTATTGCCGCATACCGATGCGAAAGACGGCATGCAGATAGCAGAACGATTGCGGCTAAACGTCAGCGCAAAAAAG
ATTGAATATCAAGGTCAGTTACTTAGCGTCACAATCAGTATAGGTTGTGCAGTATTGCGGCACAGTGAAGAGACCGCCCA
AAGCTTACTGATAGCAGCCGATAGCGCCTTATATCAAGCTAAAGCCGCTGGCCGAAATTGCGTAAAAGTCGCGGTTGACG
AACCAGACATAACTCCCACATAG

Upstream 100 bases:

>100_bases
AACAGGCTTATAGACCCAGCTTCACTGCGTTTACTCTGGTCAAAACGCCGATAACTTTCTATAGTGACAACAAATAAATA
TTATAAAGCAGTGAGTGGTT

Downstream 100 bases:

>100_bases
CCATGCTCATCAGCTCAGCCATTCGATTAACCGCGCTTAATTAAATGGTTGCTCAGGCATAAAAGGCAGGTGACTACCAG
CGGGAATCATTTTCACAGGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVA
SAYLFLIPLVTVQLLVFPGVFAPKGLLNAGAQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIP
VLCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEP
LCLLVLDVDHFKAFNDQHGHLAGDDCLVAVAKQLEQSLYRPADFIARYGGEEFVVVLPHTDAKDGMQIAERLRLNVSAKK
IEYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITPT

Sequences:

>Translated_460_residues
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVA
SAYLFLIPLVTVQLLVFPGVFAPKGLLNAGAQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIP
VLCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEP
LCLLVLDVDHFKAFNDQHGHLAGDDCLVAVAKQLEQSLYRPADFIARYGGEEFVVVLPHTDAKDGMQIAERLRLNVSAKK
IEYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITPT
>Mature_459_residues
PDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESITAYLLLSQFASNRQVFAGFVAS
AYLFLIPLVTVQLLVFPGVFAPKGLLNAGAQSAVWIWVFWHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPV
LCSLALALFATWGSHWLPILIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLFA
GARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNRRYLESQLAIHIDHAKRHKEPL
CLLVLDVDHFKAFNDQHGHLAGDDCLVAVAKQLEQSLYRPADFIARYGGEEFVVVLPHTDAKDGMQIAERLRLNVSAKKI
EYQGQLLSVTISIGCAVLRHSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITPT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=38.0368098159509, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787262, Length=229, Percent_Identity=34.4978165938865, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI145693134, Length=200, Percent_Identity=35.5, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=39.4444444444444, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=38.2352941176471, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787802, Length=266, Percent_Identity=27.8195488721804, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788381, Length=222, Percent_Identity=29.2792792792793, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788085, Length=206, Percent_Identity=28.6407766990291, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787816, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081977, Length=163, Percent_Identity=32.5153374233129, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=33.5227272727273, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788956, Length=220, Percent_Identity=30.4545454545455, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=29.7752808988764, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=26.6272189349112, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50904; Mature: 50773

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESIT
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AYLLLSQFASNRQVFAGFVASAYLFLIPLVTVQLLVFPGVFAPKGLLNAGAQSAVWIWVF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEE
WHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPVLCSLALALFATWGSHWLPI
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
RYLESQLAIHIDHAKRHKEPLCLLVLDVDHFKAFNDQHGHLAGDDCLVAVAKQLEQSLYR
HHHHHHHHEEEEHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PADFIARYGGEEFVVVLPHTDAKDGMQIAERLRLNVSAKKIEYQGQLLSVTISIGCAVLR
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHEEECCCEEEEEHHHHHHHHH
HSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITPT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
PDIYPFLSQRPHKHAAVIVALLLLTSALVISPFISIPLPDIAPFLPVYGTAVVLLESIT
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AYLLLSQFASNRQVFAGFVASAYLFLIPLVTVQLLVFPGVFAPKGLLNAGAQSAVWIWVF
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEE
WHASFPTLILLALMAQQKLTTQQVAPMQLKYWLWGFVLIPVLCSLALALFATWGSHWLPI
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LIAKNSYKQLLHSPYALLVWGLNALALVCMIRRARSNNVLYTWLSVALLASLIDVTLTLF
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
AGARFSLGWYAARISSSVSSMVLLCALLWEVNRLYVATQRANESLYQQSVKDTLTGLFNR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHH
RYLESQLAIHIDHAKRHKEPLCLLVLDVDHFKAFNDQHGHLAGDDCLVAVAKQLEQSLYR
HHHHHHHHEEEEHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
PADFIARYGGEEFVVVLPHTDAKDGMQIAERLRLNVSAKKIEYQGQLLSVTISIGCAVLR
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHEEECCCEEEEEHHHHHHHHH
HSEETAQSLLIAADSALYQAKAAGRNCVKVAVDEPDITPT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA