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The map label for this gene is purA [H]

Identifier: 126175844

GI number: 126175844

Start: 4272113

End: 4273408

Strand: Reverse

Name: purA [H]

Synonym: Sbal_3649

Alternate gene names: 126175844

Gene position: 4273408-4272113 (Counterclockwise)

Preceding gene: 126175845

Following gene: 126175843

Centisome position: 83.34

GC content: 46.99

Gene sequence:

>1296_bases
ATGGGCAAAAACGTAGTTGTTCTCGGCACCCAATGGGGTGACGAAGGAAAAGGTAAGATTGTCGACCTTCTAACAGAACA
GGCAAAATATGTAGTTCGCTATCAAGGCGGCCACAATGCTGGTCATACCTTGGTTATTGATGGTGATAAAACCGTTCTTC
ATCTGATCCCATCGGGTATCCTGCGCGATAATGTGAAATGCATTATTGGCAACGGTGTGGTGCTTGCACCTGACGCCCTG
ATGAAAGAGATCAACATGCTGAAAGATCGTGGTGTTCCTGTTGAGGAACGTCTGTTGATCTCTGAAGCGTGTCCGCTGAT
CCTACCATTCCATTGCGCCTTAGATATCGCCCGTGAAAAAGCACGTGGCAATAAAGCTATTGGTACAACTGGCCGCGGTA
TCGGACCTGCGTATGAAGACAAGATCTCCCGTCGCGGTCTGCGAGTAGGCGACTTGTTCAATGCTGAACTGTTTGCTGAA
AAGCTGAAAGAAGTTATGAAATACCATAACTTTATGCTGACAGAGTACTATCAAGTCGAAGCTGTTGATTACGAGCAAAC
GCTAGCAGATGCTTTGGCTATGGCCGATTATCTGAAGAGCATGTGTGTTGACGTGACTGAACTGTTGGATACTGCGCGTA
AAGCCGGTGAGCCAATCCTGTTTGAAGGCGCTCAAGGTACATTACTCGACATCGACCACGGAACTTATCCTTTTGTAACC
TCTTCAAATACCACTGCCGGTGGTGTTGCGACGGGTTCAGGTTTTGGTCCGCGTCACTTAGACTATGTCTTAGGTATCAT
GAAGGCTTACACCACTCGCGTGGGTGCAGGTCCATTCCCAACTGAACTGTTATGTGAAGTTGGTGATCATTTAGGTACTA
AAGGTCATGAGTTTGGTGCGACAACGGGTCGTAAGCGTCGTCCAGGTTGGTTGGATGCTGTGGCAATGCGCCGCGCAGTT
CAAATCAACAGTGTGAGTGGCTTCTGCTTGACTAAGTTAGATGTACTTGATGGTCTGAAAGAAGTGAAAATTTGTGTGGG
TTATCAGAATCCTGATGGCACTATCTCTAAAGTGACCCCGCTTGCGGCTGAAGGTTATGAACTGGTTACTCCTGTTTATG
AAACTATGCCAGGTTGGAGCGAGTCGACTTTTGGCGCGACATCCATTGAGCAACTGCCACCGGCAGCGATCAATTATATC
AAGCGTCTTGAAGAGCTATTAGAAACACCAATCGACATTATCTCTACTGGTCCTGACCGTAACGAAACCATGATTTTGGT
TAATCCGTTCAATTAA

Upstream 100 bases:

>100_bases
AGTGGTGATTCTGGTGGTTTTTGCGTAAAAAACAGCCTGCTTGCCCTTGCTTACTAAGATCTGTTAAAATTCTTTTTTAA
CCGCAACCTTGCAGCAAATA

Downstream 100 bases:

>100_bases
GACCTGTTAAGGCCGTGTTTCACGGCCTTTTTCTCTGCTTCTTTATGCCTTTCTCTGTTTAATTCTGCGGTTAGAAATTC
AGCTCAAGCTCAAGAAATAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEINMLKDRGVPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAE
KLKEVMKYHNFMLTEYYQVEAVDYEQTLADALAMADYLKSMCVDVTELLDTARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAV
QINSVSGFCLTKLDVLDGLKEVKICVGYQNPDGTISKVTPLAAEGYELVTPVYETMPGWSESTFGATSIEQLPPAAINYI
KRLEELLETPIDIISTGPDRNETMILVNPFN

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDAL
MKEINMLKDRGVPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAE
KLKEVMKYHNFMLTEYYQVEAVDYEQTLADALAMADYLKSMCVDVTELLDTARKAGEPILFEGAQGTLLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAV
QINSVSGFCLTKLDVLDGLKEVKICVGYQNPDGTISKVTPLAAEGYELVTPVYETMPGWSESTFGATSIEQLPPAAINYI
KRLEELLETPIDIISTGPDRNETMILVNPFN
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGILRDNVKCIIGNGVVLAPDALM
KEINMLKDRGVPVEERLLISEACPLILPFHCALDIAREKARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEK
LKEVMKYHNFMLTEYYQVEAVDYEQTLADALAMADYLKSMCVDVTELLDTARKAGEPILFEGAQGTLLDIDHGTYPFVTS
SNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGATTGRKRRPGWLDAVAMRRAVQ
INSVSGFCLTKLDVLDGLKEVKICVGYQNPDGTISKVTPLAAEGYELVTPVYETMPGWSESTFGATSIEQLPPAAINYIK
RLEELLETPIDIISTGPDRNETMILVNPFN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.6009389671362, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.6261682242991, Blast_Score=324, Evalue=9e-89,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.8211586901763, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=75.8700696055684, Blast_Score=680, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=41.8604651162791, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=41.8604651162791, Blast_Score=314, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.3874709976798, Blast_Score=328, Evalue=8e-91,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=43.0588235294118, Blast_Score=334, Evalue=6e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46874; Mature: 46743

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMKEINMLKDRGVPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEKLKEVMKYHNFMLTEYYQVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
AVDYEQTLADALAMADYLKSMCVDVTELLDTARKAGEPILFEGAQGTLLDIDHGTYPFVT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
TTGRKRRPGWLDAVAMRRAVQINSVSGFCLTKLDVLDGLKEVKICVGYQNPDGTISKVTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEECCCCCCHHHCCC
LAAEGYELVTPVYETMPGWSESTFGATSIEQLPPAAINYIKRLEELLETPIDIISTGPDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVNPFN
CCEEEEEECCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEQAKYVVRYQGGHNAGHTLVIDGDKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVKCIIGNGVVLAPDALMKEINMLKDRGVPVEERLLISEACPLILPFHCALDIAREK
HHCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKISRRGLRVGDLFNAELFAEKLKEVMKYHNFMLTEYYQVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
AVDYEQTLADALAMADYLKSMCVDVTELLDTARKAGEPILFEGAQGTLLDIDHGTYPFVT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGFGPRHLDYVLGIMKAYTTRVGAGPFPTELLCEVGDHLGTKGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
TTGRKRRPGWLDAVAMRRAVQINSVSGFCLTKLDVLDGLKEVKICVGYQNPDGTISKVTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHEEEEEEECCCCCCHHHCCC
LAAEGYELVTPVYETMPGWSESTFGATSIEQLPPAAINYIKRLEELLETPIDIISTGPDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCC
NETMILVNPFN
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA