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The map label for this gene is paaF [H]

Identifier: 126175777

GI number: 126175777

Start: 4208339

End: 4209076

Strand: Direct

Name: paaF [H]

Synonym: Sbal_3582

Alternate gene names: 126175777

Gene position: 4208339-4209076 (Clockwise)

Preceding gene: 126175774

Following gene: 126175781

Centisome position: 82.08

GC content: 45.93

Gene sequence:

>738_bases
ATGAGTCATATACAGGTTCAGGATGATCAGGGCGTACGTATTATCAGCTTTAATCGCCCCGATAAACGCAATGCACTTGA
TCTTAATATGTATAAACAACTGACAGAATACCTGATCGAAGGTGAAGCAGACAACGACATTCGTGCCTTTATGCTCCATG
GCCAAGATAATTGCTTCACCTCAGGCAATGATGTTGCAGACTTTTTGAAAAATAGTGACTTAGGGCCAAATCACCCCGCT
GTACGTTTCTTATTTTGCCTGCTGGAACTCAAAAAACCGCTAGTTGCCGCTGTTTCTGGCGCCGCCGTCGGCATTGGCAC
TACCGTACTACTGCACTGCGATCTGGTATACGCCGATAACACGGCGAAGTTTCAACTGCCCTTCGTCAACTTGGCACTGG
TGCCAGAAGCGGGGGCCAGCCTACTGTTACCCGAACTCGTGGGTTATCAAAAAGCGGCAGAGCTATTGCTACTCGGCGAA
AGTTTTAATGCCGAGACGGCCCACAGACTCAATATGATCAACGAAATTGTGGCCCATGAAGACCTACTCAGTTATGCATT
GTCTCAGGCTAAAAAGTTGGCAAATCAACCGCCACAAGCCCTACAAATCACACGTCAATTAATGCGTCCGCATAAAAATC
GTGTACAACACCAAATGCATCAAGAATTAGAACAATTTAGCGCTAGGCTAAAAAGTGATGAAGCCAAGAGCAGATTTCAG
GCTTTTTTGAAGAAGTAG

Upstream 100 bases:

>100_bases
ATGCACAAGATAAAACTAGACAATTAAACAGCTGTTTGAATATTGGTTTCAATGTCTTAAACTCCATGTCTACCCAATGT
CGTCGTAAAGGAAACAACCC

Downstream 100 bases:

>100_bases
TCTGCAAGCAGTATGCACATACATGTCGCATGAGGGTCAAAGTGCCCTCTTGTTGATAGGTTTAACACCCTATTTTTTTA
AAAAATTCTTTCAATTTTTC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSHIQVQDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGQDNCFTSGNDVADFLKNSDLGPNHPA
VRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGE
SFNAETAHRLNMINEIVAHEDLLSYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKSRFQ
AFLKK

Sequences:

>Translated_245_residues
MSHIQVQDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGQDNCFTSGNDVADFLKNSDLGPNHPA
VRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGE
SFNAETAHRLNMINEIVAHEDLLSYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKSRFQ
AFLKK
>Mature_244_residues
SHIQVQDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGQDNCFTSGNDVADFLKNSDLGPNHPAV
RFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGES
FNAETAHRLNMINEIVAHEDLLSYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKSRFQA
FLKK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=30.6772908366534, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=30.6772908366534, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=30.6772908366534, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=27.4193548387097, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI25453481, Length=230, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538573, Length=230, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757966, Length=230, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538581, Length=230, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757968, Length=230, Percent_Identity=25.2173913043478, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI49355787, Length=230, Percent_Identity=25.2173913043478, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI221136756, Length=223, Percent_Identity=25.1121076233184, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221136753, Length=223, Percent_Identity=25.1121076233184, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221307494, Length=213, Percent_Identity=25.8215962441315, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI116235487, Length=214, Percent_Identity=24.2990654205607, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI31542718, Length=219, Percent_Identity=25.1141552511416, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=27.1739130434783, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI213417737, Length=212, Percent_Identity=24.0566037735849, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI157694516, Length=212, Percent_Identity=24.0566037735849, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4502327, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=31.8548387096774, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=35.1351351351351, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI221142681, Length=241, Percent_Identity=27.3858921161826, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788597, Length=201, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=27.5675675675676, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=26.7015706806283, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=29.3388429752066, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=26.530612244898, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=239, Percent_Identity=26.3598326359833, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=32.6829268292683, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=30.952380952381, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=24.901185770751, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=227, Percent_Identity=23.3480176211454, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=212, Percent_Identity=24.5283018867925, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=206, Percent_Identity=23.7864077669903, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=219, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=30.6603773584906, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=28.5123966942149, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=28.5123966942149, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653139, Length=195, Percent_Identity=30.2564102564103, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650670, Length=220, Percent_Identity=30, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357171, Length=218, Percent_Identity=27.9816513761468, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27432; Mature: 27301

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIQVQDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGQDNCFT
CCCCEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SGNDVADFLKNSDLGPNHPAVRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADN
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEEEEECC
TAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGESFNAETAHRLNMINEIVAHE
CCEEEECEEEEEECCCCCCHHHHHHHHCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHH
DLLSYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKSRFQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKK
HHHCC
>Mature Secondary Structure 
SHIQVQDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGQDNCFT
CCCEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SGNDVADFLKNSDLGPNHPAVRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADN
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEEEEECC
TAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGESFNAETAHRLNMINEIVAHE
CCEEEECEEEEEECCCCCCHHHHHHHHCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHH
DLLSYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKSRFQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]