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The map label for this gene is citS [H]

Identifier: 126175629

GI number: 126175629

Start: 4023224

End: 4024837

Strand: Reverse

Name: citS [H]

Synonym: Sbal_3432

Alternate gene names: 126175629

Gene position: 4024837-4023224 (Counterclockwise)

Preceding gene: 126175630

Following gene: 126175628

Centisome position: 78.5

GC content: 49.63

Gene sequence:

>1614_bases
ATGAGACGCCCTAAGCGCTTAGAAACGTGGTTAATCAGTGGGGTGACTTGCCTGAGTTTGTTACTGTGCGCCTTATTTGG
CGGCATCAGCTATCAAATTTTGAGTCGCAGTATTACTGAGCAATCGGGAGCTAAGGCATTAGGGCTGGCCCATGCGGTCG
CGACACGGACCGATGTGATTACCGCTTTGCAAACGGGGCATTCGAGCCCACAATTGCTGGCCGCGATAGAAGGAATACGT
CTAGCGTCTAAGGCCGATTTTATCGTGGTGGGCGATACTCATAAATATCGTATCGTGCACCCAGATTCAGATAAAATTGG
CAAGAAGATGGAGGGCGGTGATAGCCAAGCGGCATTAGAAGGGGAGTCTTATATCTCCGAGGCGCAGGGCAGTTTAGGTC
GCTCTATTCGTGGTAAAGTGCCAGTGCTTGACGAGCAAGGCCAAATTATCGGCTTGGTTTCGGTGGGCTTTTTGAGCCAG
TCCGTGGGCGCGTTAATCGATAAACGTATTCCTGAAATCATCATGACAGTCCTAGGAGTGTTATTGATGGGCGTGCTGGT
GGCTTTTTGGATGGGGCGGCAAGTGCGGGAAATGTTGTTCGGCTTACAACCTGCGGAAATTGGCCGTTTATTCGTCGAGC
AGGACGCCATTCTTAACACAGTGCGAATGGGCGTGGTGGCATTGGATGCCGATGGCCGTATCCGCAAGTTGAATCAAAGG
GCGCGGGAGATTTTAGCTTTATCTCCGACCTGCCAACCTAATACTCTGATGTTGAGTGATTTATTGCCGCAGCACGCCGA
CTTTCTACTGACGAACCAAAACAAAACCATCAGGGGATTCGAGTTATTTGCCAATGATCAATGGCTGGTGATGTCGCGGG
TGCCGTTTCAGGTGCGGGATCAAAATGATGGCTTGCTGCTGACGATGCGCCCCGCCGATGAAATTGAGCGCTTGAGCCAA
CAACTGGCCAAAGTGCAAGAGTTTGCCGAAATGCTCAGAGTGCAAACCCATGATTACTCGAATAAACTCCATACTCTAGG
CGCCTTGTTGCAACTTAAAGCCTACGACAAAGCGGTCGAATTTATCGGCCAAGAAAGCCAAGGTTGGCAGGCTCAAATCC
ATTTATTACTGTCGCAAATCCATGAGCCGACGATCGCGGGATTACTCTTGGGTAAGTATCACAAGGCGAAAGAGCTCAAT
ATTGAGTTTCAAATCAGCCCAGATAGTCAACTCAGCGATGTGCAAGACGCCATTATGCTTGAGCGGATGGTGTCGATTAT
GGGCAACCTTATCGATAACGCCATTGAGGCTGTGCACAGGGCGAACAATCATAAGCCCCGCCGCGTGGTCGTGACCTTAG
ATGAAACTGCCTCTAACCTGATTTTTGATGTGGAAGACAGTGGCGATGGGATTTCTAAAACCGAGTACAGTACGATATTC
GATCCAGATTACAGCTCTAAGCAGGGGGCGCAGCACGGTGTGGGCATGTATATCGTTAATACTTACCTGTGTGCCTGCCA
TGGCAGCTTAGAGTTTGCCGACTCAGATTTAGGTGGAGCGCGTTTTTCTGTGTATATTCCCACTAAACCTTTACCTGAAG
TAGGTACCGTTTAA

Upstream 100 bases:

>100_bases
GATTGTGGCAATTGCGATGCTGGCTGTTCCATTGATCGGTTTTATTCGTAGTCATCGTAGTGATAAATTGGCGAAAGCCT
CTTAATGTAAAGGTACGCTA

Downstream 100 bases:

>100_bases
TGCATACCGTCATGATTGTTGCGGACCAAGTTGCGCGTGTTGCTGAGTTAACCGCACCGTATTTGTTTCCTGAACTTCTG
CCTGAAGTAGGTATTGTTTA

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MRRPKRLETWLISGVTCLSLLLCALFGGISYQILSRSITEQSGAKALGLAHAVATRTDVITALQTGHSSPQLLAAIEGIR
LASKADFIVVGDTHKYRIVHPDSDKIGKKMEGGDSQAALEGESYISEAQGSLGRSIRGKVPVLDEQGQIIGLVSVGFLSQ
SVGALIDKRIPEIIMTVLGVLLMGVLVAFWMGRQVREMLFGLQPAEIGRLFVEQDAILNTVRMGVVALDADGRIRKLNQR
AREILALSPTCQPNTLMLSDLLPQHADFLLTNQNKTIRGFELFANDQWLVMSRVPFQVRDQNDGLLLTMRPADEIERLSQ
QLAKVQEFAEMLRVQTHDYSNKLHTLGALLQLKAYDKAVEFIGQESQGWQAQIHLLLSQIHEPTIAGLLLGKYHKAKELN
IEFQISPDSQLSDVQDAIMLERMVSIMGNLIDNAIEAVHRANNHKPRRVVVTLDETASNLIFDVEDSGDGISKTEYSTIF
DPDYSSKQGAQHGVGMYIVNTYLCACHGSLEFADSDLGGARFSVYIPTKPLPEVGTV

Sequences:

>Translated_537_residues
MRRPKRLETWLISGVTCLSLLLCALFGGISYQILSRSITEQSGAKALGLAHAVATRTDVITALQTGHSSPQLLAAIEGIR
LASKADFIVVGDTHKYRIVHPDSDKIGKKMEGGDSQAALEGESYISEAQGSLGRSIRGKVPVLDEQGQIIGLVSVGFLSQ
SVGALIDKRIPEIIMTVLGVLLMGVLVAFWMGRQVREMLFGLQPAEIGRLFVEQDAILNTVRMGVVALDADGRIRKLNQR
AREILALSPTCQPNTLMLSDLLPQHADFLLTNQNKTIRGFELFANDQWLVMSRVPFQVRDQNDGLLLTMRPADEIERLSQ
QLAKVQEFAEMLRVQTHDYSNKLHTLGALLQLKAYDKAVEFIGQESQGWQAQIHLLLSQIHEPTIAGLLLGKYHKAKELN
IEFQISPDSQLSDVQDAIMLERMVSIMGNLIDNAIEAVHRANNHKPRRVVVTLDETASNLIFDVEDSGDGISKTEYSTIF
DPDYSSKQGAQHGVGMYIVNTYLCACHGSLEFADSDLGGARFSVYIPTKPLPEVGTV
>Mature_537_residues
MRRPKRLETWLISGVTCLSLLLCALFGGISYQILSRSITEQSGAKALGLAHAVATRTDVITALQTGHSSPQLLAAIEGIR
LASKADFIVVGDTHKYRIVHPDSDKIGKKMEGGDSQAALEGESYISEAQGSLGRSIRGKVPVLDEQGQIIGLVSVGFLSQ
SVGALIDKRIPEIIMTVLGVLLMGVLVAFWMGRQVREMLFGLQPAEIGRLFVEQDAILNTVRMGVVALDADGRIRKLNQR
AREILALSPTCQPNTLMLSDLLPQHADFLLTNQNKTIRGFELFANDQWLVMSRVPFQVRDQNDGLLLTMRPADEIERLSQ
QLAKVQEFAEMLRVQTHDYSNKLHTLGALLQLKAYDKAVEFIGQESQGWQAQIHLLLSQIHEPTIAGLLLGKYHKAKELN
IEFQISPDSQLSDVQDAIMLERMVSIMGNLIDNAIEAVHRANNHKPRRVVVTLDETASNLIFDVEDSGDGISKTEYSTIF
DPDYSSKQGAQHGVGMYIVNTYLCACHGSLEFADSDLGGARFSVYIPTKPLPEVGTV

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=493, Percent_Identity=30.8316430020284, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790567, Length=528, Percent_Identity=29.5454545454545, Blast_Score=216, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59108; Mature: 59108

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPKRLETWLISGVTCLSLLLCALFGGISYQILSRSITEQSGAKALGLAHAVATRTDVI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TALQTGHSSPQLLAAIEGIRLASKADFIVVGDTHKYRIVHPDSDKIGKKMEGGDSQAALE
HHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCCHHCC
GESYISEAQGSLGRSIRGKVPVLDEQGQIIGLVSVGFLSQSVGALIDKRIPEIIMTVLGV
CHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMGVLVAFWMGRQVREMLFGLQPAEIGRLFVEQDAILNTVRMGVVALDADGRIRKLNQR
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
AREILALSPTCQPNTLMLSDLLPQHADFLLTNQNKTIRGFELFANDQWLVMSRVPFQVRD
HHHHEEECCCCCCCCEEHHHHCCCCCCEEEECCCCCEEEEEEEECCCEEEEECCCEEEEC
QNDGLLLTMRPADEIERLSQQLAKVQEFAEMLRVQTHDYSNKLHTLGALLQLKAYDKAVE
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FIGQESQGWQAQIHLLLSQIHEPTIAGLLLGKYHKAKELNIEFQISPDSQLSDVQDAIML
HHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHH
ERMVSIMGNLIDNAIEAVHRANNHKPRRVVVTLDETASNLIFDVEDSGDGISKTEYSTIF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEE
DPDYSSKQGAQHGVGMYIVNTYLCACHGSLEFADSDLGGARFSVYIPTKPLPEVGTV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MRRPKRLETWLISGVTCLSLLLCALFGGISYQILSRSITEQSGAKALGLAHAVATRTDVI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TALQTGHSSPQLLAAIEGIRLASKADFIVVGDTHKYRIVHPDSDKIGKKMEGGDSQAALE
HHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEEECCCCHHHCCCCCCCCCCHHCC
GESYISEAQGSLGRSIRGKVPVLDEQGQIIGLVSVGFLSQSVGALIDKRIPEIIMTVLGV
CHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMGVLVAFWMGRQVREMLFGLQPAEIGRLFVEQDAILNTVRMGVVALDADGRIRKLNQR
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHH
AREILALSPTCQPNTLMLSDLLPQHADFLLTNQNKTIRGFELFANDQWLVMSRVPFQVRD
HHHHEEECCCCCCCCEEHHHHCCCCCCEEEECCCCCEEEEEEEECCCEEEEECCCEEEEC
QNDGLLLTMRPADEIERLSQQLAKVQEFAEMLRVQTHDYSNKLHTLGALLQLKAYDKAVE
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FIGQESQGWQAQIHLLLSQIHEPTIAGLLLGKYHKAKELNIEFQISPDSQLSDVQDAIML
HHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHH
ERMVSIMGNLIDNAIEAVHRANNHKPRRVVVTLDETASNLIFDVEDSGDGISKTEYSTIF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEE
DPDYSSKQGAQHGVGMYIVNTYLCACHGSLEFADSDLGGARFSVYIPTKPLPEVGTV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]