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The map label for this gene is cysN [H]

Identifier: 126175604

GI number: 126175604

Start: 3990767

End: 3992209

Strand: Reverse

Name: cysN [H]

Synonym: Sbal_3407

Alternate gene names: 126175604

Gene position: 3992209-3990767 (Counterclockwise)

Preceding gene: 126175605

Following gene: 126175603

Centisome position: 77.86

GC content: 48.86

Gene sequence:

>1443_bases
ATGGATAAAGCGGTCAATAAAACCAATCTCATGGCGGCAGAAATTACCCTCCATGGTGTGAAAGAATATTTAGCCCTGCA
GCAGCGCAAAGGCTTATTGCGCTTTTTAACCTGTGGCAGTGTGGATGACGGCAAAAGTACCTTAATCGGTCGCCTGTTAC
ACGACAGTGCCCAGATCTATGAAGATCAATTGGCGAGCCTAAAGAGCGACAGCGCTAAGATGGGCACCACAGGTGAAGCT
ATCGACTTAGCCTTGCTGGTGGATGGTCTGCAAGCCGAGCGCGAGCAGGGCATCACTATCGATGTGGCTTACCGTTACTT
TTCAAGTGATAAGCGTAAGTTCATCATCGCCGATACGCCAGGGCATGAGCAGTACACGCGTAACATGGCAACGGGTGCAT
CGACCTGTGATTTGGCTGTGATTTTAGTCGATGCCCGCTACGGCGTGCAGACTCAGACTAAACGCCATGCGTTTATCGCG
TCATTACTGGGGATTCGTCACTTCGTTGTTGCCGTGAACAAGATGGATTTGCTTGGGTTTGACGAGCAAGTATTCAATCG
CATTCGTGCTGACTTTACCGATTTCGTTAAAGATTTCGGTAACCTCGATATCCACTTTGTGCCGTTATCGGCGCTTAATG
GTGACAACGTGGTTGAGCGCAGTACTCAAAGCGACTGGTATCAAGGTGGTACTCTGCTGGAACTGCTAGAAACCATTGAT
ACTCAACGTGAACTCAGCTCATTGCCAGTGCGTTTCCCTGTGCAATATGTGTCGCGTCCTAACTTGGATTTTCGCGGTTT
TGCAGGCACTTTAGCCTCTGGCATTATCAATGTCGGTGACGAGTTAGTCGCTTTGCCATCGGGCAAACGCAGCAAGGTTG
AACGTATTGTGACCTTCGATGGTGACATACAAGAAGCGGTTGCTGGCCAAGCTGTGACTATCACGCTGGAAGATGAAATT
GATATTTCCCGTGGCGATTTACTGGCAAAACCCGATTCTGCGCCAGCCGTCGCTAATGTAATCAGCGCTGACATAGTGTG
GATGGATGAAAAGCCATTACAACTCGGTCAGTTATACGACATCAAAGTGGCCGGTAAGAAGACGCAAGCCTCTGTGACTG
GCATCGATTATGTGGTTGATGTTAATACTTTAGCGCGCAGCGCGACGGACACGCTTGGGTTAAACGCGATTGCTCGCGTG
ACCTTAGAGCTGACCGAAGACATAGTGTTCGATGCGTATTCATTAGTGCGTGATACCGGCGGAATGATCCTAATCGATCG
CCTGAGCAATGCGACGGTTGCCGCAGTGATGGTTGTGTCGGGTCAGCATGTTAACAAGCAAACAGCATCACAATTTAGTG
CCTTTGAAGTTGAGTTTAATGCCTTAGTGCGTAAACACTACCCACATTGGCAAGCGATAGATATCTCTAAGCTTGGAGCT
TAA

Upstream 100 bases:

>100_bases
GTCAAGGCTACTTCTAATCTCGGCAAGGTTAACCCTATTTCGCGAAGGTGGCGCAAGTAAAACGCCACCTTCAGCCAAGC
AGATCAAGAGTGAGAATAAG

Downstream 100 bases:

>100_bases
TTTGTGAGTGACTTGTGGCTGTTAGCGATTATTTTGTTAGGTCTTGTGGCTGGCTTAATTAGCGGCCGTATCAATCCCGC
GGTATTGTTTTTATTTGCAT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQRKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTGIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA

Sequences:

>Translated_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQRKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTGIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
>Mature_480_residues
MDKAVNKTNLMAAEITLHGVKEYLALQQRKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKSDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFDGDIQEAVAGQAVTITLEDEI
DISRGDLLAKPDSAPAVANVISADIVWMDEKPLQLGQLYDIKVAGKKTQASVTGIDYVVDVNTLARSATDTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=29.4252873563218, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=29.4252873563218, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503475, Length=336, Percent_Identity=29.4642857142857, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI4503471, Length=360, Percent_Identity=28.6111111111111, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI194018522, Length=329, Percent_Identity=29.7872340425532, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI194097354, Length=418, Percent_Identity=27.511961722488, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI194018520, Length=418, Percent_Identity=27.511961722488, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=29.1793313069909, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI34147630, Length=314, Percent_Identity=30.2547770700637, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI157426893, Length=348, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789108, Length=460, Percent_Identity=63.695652173913, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1790412, Length=314, Percent_Identity=28.0254777070064, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789737, Length=314, Percent_Identity=28.0254777070064, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367247, Length=256, Percent_Identity=27.34375, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=360, Percent_Identity=30.5555555555556, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=360, Percent_Identity=30.5555555555556, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI32566629, Length=419, Percent_Identity=27.4463007159904, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115532067, Length=424, Percent_Identity=26.8867924528302, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=424, Percent_Identity=26.8867924528302, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=353, Percent_Identity=28.8951841359773, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=235, Percent_Identity=27.6595744680851, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=352, Percent_Identity=24.7159090909091, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=346, Percent_Identity=30.0578034682081, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=346, Percent_Identity=30.0578034682081, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=408, Percent_Identity=29.9019607843137, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=314, Percent_Identity=28.343949044586, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324761, Length=339, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=165, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550900, Length=427, Percent_Identity=25.7611241217799, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24652838, Length=316, Percent_Identity=29.4303797468354, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137572, Length=316, Percent_Identity=29.4303797468354, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553807, Length=277, Percent_Identity=30.6859205776173, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45553816, Length=277, Percent_Identity=30.6859205776173, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24651721, Length=277, Percent_Identity=30.6859205776173, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17864154, Length=277, Percent_Identity=30.6859205776173, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=424, Percent_Identity=25.2358490566038, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281363316, Length=315, Percent_Identity=28.8888888888889, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17864358, Length=315, Percent_Identity=28.8888888888889, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921738, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52519; Mature: 52519

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQRKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHH
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFD
HHHHHHHCCCEECHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDIQEAVAGQAVTITLEDEIDISRGDLLAKPDSAPAVANVISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCCCCCCCEEE
IKVAGKKTQASVTGIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEECCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHCCCCCEEECHHHCCC
>Mature Secondary Structure
MDKAVNKTNLMAAEITLHGVKEYLALQQRKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKSDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCHHCCCC
DEQVFNRIRADFTDFVKDFGNLDIHFVPLSALNGDNVVERSTQSDWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCCCHHHCCCHHHHHHHHHH
TQRELSSLPVRFPVQYVSRPNLDFRGFAGTLASGIINVGDELVALPSGKRSKVERIVTFD
HHHHHHHCCCEECHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
GDIQEAVAGQAVTITLEDEIDISRGDLLAKPDSAPAVANVISADIVWMDEKPLQLGQLYD
CCHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCCCCCCCEEE
IKVAGKKTQASVTGIDYVVDVNTLARSATDTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCCCEECCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQTASQFSAFEVEFNALVRKHYPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHEEEEHHHHHHHHCCCCCEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]