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The map label for this gene is pth

Identifier: 126175458

GI number: 126175458

Start: 3819929

End: 3820516

Strand: Reverse

Name: pth

Synonym: Sbal_3258

Alternate gene names: 126175458

Gene position: 3820516-3819929 (Counterclockwise)

Preceding gene: 126175459

Following gene: 126175457

Centisome position: 74.51

GC content: 47.11

Gene sequence:

>588_bases
ATGAGCGAAATAAAATTAATAGTTGGTCTGGCTAACCCTGGTGCTGAATATGCGCACACTCGCCATAATGCGGGGGCTTG
GTACGTACTAGAGTTGGCTCGAATTTGCGGCGTGACTTTAGTGGCCGACAGTAAGTATTTTGGTTTGACCGCCAGAGCCG
TATTGCACGGTAAAGATGTCCGCTTATTGATCCCGACGACTTATATGAATTTAAGTGGTAAAGCTGTGGGTGCTTTGGCG
AACTTTTTTCGTATCACACCGGAAGAGATTCTGGTCGCTCACGATGAACTCGATCTGCCGCCCGGAGTGGCTAAGTTCAA
GCTCGGTGGCGGCCATGGCGGCCACAATGGTTTGAAAGATATTATTGCTAAACTGGCTAACGATAAAAACTTCTACCGTT
TACGCTTAGGTATCGGTCATCCCGGTGATAAAAACCAAGTGAGTGGTTACGTACTGGGAAAAGCACCTGCTAAAGAGCAA
GAGCTTATCGATGCGGCGATAGATGAAGCCGTGCGTTCTACCGAGATTTTGTTTAAGCAAGACATGGTGAAAGCCATGAA
CAGATTACACTCCTTTAAAGCGGAGTAG

Upstream 100 bases:

>100_bases
TTGGGCATTGAGTGCTAAAATGCCGCGTTTTTAGCAGCTATGGTCAGTTCAGTCGGCGCTTTGCGCGGCTGCCATCGACG
AAATAACGATATTGAGTGTC

Downstream 100 bases:

>100_bases
GTTTTGCCTTTGGCGAGACTCAAGTTAATTAACTAATTAATTCAGTAATGTGCTGCCATAGGGCAGGACGACTAAACCTC
GCTGATGAGTGTGACTTGTC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEIKLIVGLANPGAEYAHTRHNAGAWYVLELARICGVTLVADSKYFGLTARAVLHGKDVRLLIPTTYMNLSGKAVGALA
NFFRITPEEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIIAKLANDKNFYRLRLGIGHPGDKNQVSGYVLGKAPAKEQ
ELIDAAIDEAVRSTEILFKQDMVKAMNRLHSFKAE

Sequences:

>Translated_195_residues
MSEIKLIVGLANPGAEYAHTRHNAGAWYVLELARICGVTLVADSKYFGLTARAVLHGKDVRLLIPTTYMNLSGKAVGALA
NFFRITPEEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIIAKLANDKNFYRLRLGIGHPGDKNQVSGYVLGKAPAKEQ
ELIDAAIDEAVRSTEILFKQDMVKAMNRLHSFKAE
>Mature_194_residues
SEIKLIVGLANPGAEYAHTRHNAGAWYVLELARICGVTLVADSKYFGLTARAVLHGKDVRLLIPTTYMNLSGKAVGALAN
FFRITPEEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIIAKLANDKNFYRLRLGIGHPGDKNQVSGYVLGKAPAKEQE
LIDAAIDEAVRSTEILFKQDMVKAMNRLHSFKAE

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=38.6904761904762, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1787455, Length=192, Percent_Identity=71.875, Blast_Score=263, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_SHEB2 (B8E6L3)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357047.1
- ProteinModelPortal:   B8E6L3
- SMR:   B8E6L3
- GeneID:   7087562
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1109
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21185; Mature: 21054

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKLIVGLANPGAEYAHTRHNAGAWYVLELARICGVTLVADSKYFGLTARAVLHGKDV
CCCEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCE
RLLIPTTYMNLSGKAVGALANFFRITPEEILVAHDELDLPPGVAKFKLGGGHGGHNGLKD
EEEEEEEEECCCCHHHHHHHHHHHCCHHHHEEECCCCCCCCCCEEEEECCCCCCCCCHHH
IIAKLANDKNFYRLRLGIGHPGDKNQVSGYVLGKAPAKEQELIDAAIDEAVRSTEILFKQ
HHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DMVKAMNRLHSFKAE
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEIKLIVGLANPGAEYAHTRHNAGAWYVLELARICGVTLVADSKYFGLTARAVLHGKDV
CCEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCE
RLLIPTTYMNLSGKAVGALANFFRITPEEILVAHDELDLPPGVAKFKLGGGHGGHNGLKD
EEEEEEEEECCCCHHHHHHHHHHHCCHHHHEEECCCCCCCCCCEEEEECCCCCCCCCHHH
IIAKLANDKNFYRLRLGIGHPGDKNQVSGYVLGKAPAKEQELIDAAIDEAVRSTEILFKQ
HHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DMVKAMNRLHSFKAE
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA