Click here to switch to the map view.

The map label for this gene is ubiF [H]

Identifier: 126175459

GI number: 126175459

Start: 3820669

End: 3821961

Strand: Reverse

Name: ubiF [H]

Synonym: Sbal_3259

Alternate gene names: 126175459

Gene position: 3821961-3820669 (Counterclockwise)

Preceding gene: 126175468

Following gene: 126175458

Centisome position: 74.54

GC content: 48.72

Gene sequence:

>1293_bases
ATGAGCCAAATTGATGAAGCAAGTGCACCGATGACATCCGATACAACTCATTCTGACGTCACTCATGTTGATGTGACTCA
TTCAGACGTCACTCATGTTAAAGGCACTCATGTTGAAGACGCTGAGTTCGATGTCATTCTCGTCGGCGGCGGTATGGTCG
GTGCAGCGACGGCAATTGGATTAGCCCAACAGGGCTTGCAAGTGGCGGTGATTGAATCATTTGCGCCAGAGGCTTATCAA
GCCGAGCAGCCGTTAGATGTGCGAGTCTCGGCCATCAGTGTGACTTCTGAAACGCTGCTTGAACGTCTTGGCGCCTTGGA
CTCACTGCTGAAAATGCGCAATGTGCCTTATCTTGGCTTAGAAACTTGGGAGCTTGATGGCTGCATCACCCAATTCCACA
GCTCGCAAATTGGCGCTAGTCATTTAGGTCATATCGTTGAAAACCGTTTAATCCAACTTGCCCTGTGGCAACAAATGACG
CAATTGGCGGGCATTACTTTATATTGCCCCGATAAAGTGGTGGCATTTGCCCGTGATAGTGATGGCGTCAGTGTGCACTT
ACAATCGAATAGTAAACTTAACGCTAAGCTATTGGTCGGTGCCGATGGTGCACACTCGCAGGTACGCCAATGGGCTGGGA
TTGGTATTTCAGGGTGGGATTATGCTCAATCGGCGATGCTGATCAATATCAATACCGCACAGGGCCAGCAAGATGTCACT
TGGCAGCAGTTTACGCCAAAAGGACCGCGTTCATTATTACCTCTGCCCGGCAACAATGCTTCGCTGGTGTGGTACGACGA
CGCCAACCGAATTAAACAGTTAATGCAGCTTAATCACAAACAGTTGGCCGAACAGATACGTTTGCATTTCCCTGCGCGCT
TAGATGCCGATTTCACCGTTGAGAATAAAGGCAGTTTTGGCCTCACTCGTCGCCATGCGCAGCGTTACTATAGTCAGAAC
GTGGTGATTTTAGGGGATGCCGCCCACACCATTAATCCGCTCGCTGGCCAAGGGGTGAACCTTGGGTTTAAGGATGTTGA
AGCGTTTTTGGCGGCCATTAAGGATGCATTAGCCCAAGATAATATCTGGTGGTCGAATGAAGTGCTTGCCCAATATCAGC
GCAAACGCTATCGCGATAACCAACTGATGATGACGGCGATGGATTTGTTTTACGCGGGTTTTAGTAACGATATCCTGCCG
CTTAAGCTGCTGCGTAATGGCGCGTTAAAGTTGGCAAACATCGATTCTCCAATCAAAAAGACCGTTCTTAAATATGCAAT
GGGGCTTAATTAA

Upstream 100 bases:

>100_bases
TGCAGTCGGTGTAAAATAGCTGGCGCATAGACTGGCGCATAGATAGTGTAAAGACGTCGCTTGGGCAGTAACAAACAAGG
CAATAAAGGTGGTAAGTAAG

Downstream 100 bases:

>100_bases
TATCGAGTTGCTCTGCGTTCAGACTCAGTGCGATGACGTGGCATTTGTTGACTTGGGCATTGAGTGCTAAAATGCCGCGT
TTTTAGCAGCTATGGTCAGT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSQIDEASAPMTSDTTHSDVTHVDVTHSDVTHVKGTHVEDAEFDVILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQ
AEQPLDVRVSAISVTSETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMT
QLAGITLYCPDKVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVT
WQQFTPKGPRSLLPLPGNNASLVWYDDANRIKQLMQLNHKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQN
VVILGDAAHTINPLAGQGVNLGFKDVEAFLAAIKDALAQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILP
LKLLRNGALKLANIDSPIKKTVLKYAMGLN

Sequences:

>Translated_430_residues
MSQIDEASAPMTSDTTHSDVTHVDVTHSDVTHVKGTHVEDAEFDVILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQ
AEQPLDVRVSAISVTSETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMT
QLAGITLYCPDKVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVT
WQQFTPKGPRSLLPLPGNNASLVWYDDANRIKQLMQLNHKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQN
VVILGDAAHTINPLAGQGVNLGFKDVEAFLAAIKDALAQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILP
LKLLRNGALKLANIDSPIKKTVLKYAMGLN
>Mature_429_residues
SQIDEASAPMTSDTTHSDVTHVDVTHSDVTHVKGTHVEDAEFDVILVGGGMVGAATAIGLAQQGLQVAVIESFAPEAYQA
EQPLDVRVSAISVTSETLLERLGALDSLLKMRNVPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMTQ
LAGITLYCPDKVVAFARDSDGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVTW
QQFTPKGPRSLLPLPGNNASLVWYDDANRIKQLMQLNHKQLAEQIRLHFPARLDADFTVENKGSFGLTRRHAQRYYSQNV
VILGDAAHTINPLAGQGVNLGFKDVEAFLAAIKDALAQDNIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPL
KLLRNGALKLANIDSPIKKTVLKYAMGLN

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=387, Percent_Identity=30.4909560723514, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI32967307, Length=330, Percent_Identity=30.3030303030303, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1786883, Length=386, Percent_Identity=47.6683937823834, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI1789273, Length=390, Percent_Identity=38.4615384615385, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789274, Length=402, Percent_Identity=28.3582089552239, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25146631, Length=434, Percent_Identity=26.7281105990783, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321694, Length=449, Percent_Identity=24.9443207126949, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920760, Length=449, Percent_Identity=26.7260579064588, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 47267; Mature: 47136

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIDEASAPMTSDTTHSDVTHVDVTHSDVTHVKGTHVEDAEFDVILVGGGMVGAATAIG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
LAQQGLQVAVIESFAPEAYQAEQPLDVRVSAISVTSETLLERLGALDSLLKMRNVPYLGL
HHHCCCEEEEEHHCCCCHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECC
ETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCPDKVVAFARDS
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECC
DGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVT
CCEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC
WQQFTPKGPRSLLPLPGNNASLVWYDDANRIKQLMQLNHKQLAEQIRLHFPARLDADFTV
HHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEE
ENKGSFGLTRRHAQRYYSQNVVILGDAAHTINPLAGQGVNLGFKDVEAFLAAIKDALAQD
ECCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
NIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALKLANIDSPIKK
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHH
TVLKYAMGLN
HHHHHHHCCC
>Mature Secondary Structure 
SQIDEASAPMTSDTTHSDVTHVDVTHSDVTHVKGTHVEDAEFDVILVGGGMVGAATAIG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
LAQQGLQVAVIESFAPEAYQAEQPLDVRVSAISVTSETLLERLGALDSLLKMRNVPYLGL
HHHCCCEEEEEHHCCCCHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEECC
ETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMTQLAGITLYCPDKVVAFARDS
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECC
DGVSVHLQSNSKLNAKLLVGADGAHSQVRQWAGIGISGWDYAQSAMLININTAQGQQDVT
CCEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC
WQQFTPKGPRSLLPLPGNNASLVWYDDANRIKQLMQLNHKQLAEQIRLHFPARLDADFTV
HHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEE
ENKGSFGLTRRHAQRYYSQNVVILGDAAHTINPLAGQGVNLGFKDVEAFLAAIKDALAQD
ECCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
NIWWSNEVLAQYQRKRYRDNQLMMTAMDLFYAGFSNDILPLKLLRNGALKLANIDSPIKK
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCCHHHH
TVLKYAMGLN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]