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The map label for this gene is topB [H]

Identifier: 126174943

GI number: 126174943

Start: 3209796

End: 3211913

Strand: Reverse

Name: topB [H]

Synonym: Sbal_2736

Alternate gene names: 126174943

Gene position: 3211913-3209796 (Counterclockwise)

Preceding gene: 126174945

Following gene: 126174941

Centisome position: 62.64

GC content: 49.15

Gene sequence:

>2118_bases
ATGATCCTCTATATCGCTGAAAAACCTAGCCTAGGGCGGGCCATTGCCGATGTATTGCCTAAACCCCATAAAAAAGGCGA
TGGATTTATTGAAGCCGCCAATGGCGATTGCGTGTCTTGGTGTGTCGGTCACTTGCTCGAACAAGCAGAACCTGATGCCT
ATAATCCCGAATTTAAGTCGTGGAAATTCGAGCACTTGCCAATAGTGCCGGACAAGTGGCAACTAAAACCTAAGGCCGCA
ACTCGCAGCCAACTCACAGTGCTGAAACGATTAGTCAAACAAGCTAACACGCTGGTTAATGCAGGCGATCCCGATCGTGA
AGGGCAATTACTCGTGGATGAAGTGATTGCCTATCTTGGCGTGACGGGTGATAAATTACATCAAACTCAGCGTTTACTGA
TCAGCGACTTAAACCCACAAGCCGTAAAACGGGCGCTAACGCAGCTACGCAGTAATCGCGAGTTTATTCCTTTGTCCACA
TCGGCCTTAGCCCGTAGCCGCGCCGATTGGCTCTACGGCATGAATATGACCCGCGCGTACACTATCCAAGGTAAAAAGGT
TGGTTATCAAGGCGTATTGTCCGTCGGGCGAGTGCAAACGCCTTTACTCGGGCTAGTGGTGCGCCGTGATGAAGAGATTG
CTAATTTTCAATCTAAACCTTTCTATGAAGTGCTAGCGCATTTGGCGACCGAAAAGCAGGAAACCTTCAGTGCTAAGTGG
CAACCCAGCGAAGCTTGTCAGCCCTATATGGACGAAGAAGGGCGAGTATTAGCCAGAGGATTAGCGCAAAATGTGGTGAG
TCGCATCAGTGATAAACCTGCCTTAGTGACTCAATTAGTGGCTAAGGACAAGAAACAGAATCCGCCTTTGCCCTACAGCT
TATCCGCGTTGCAAATTGATGCGGCAAAACGGTTTGGCATGAGTGCTAAGGATGTGCTCGATACCTGCCAGAGTTTGTAT
GAGCGCCATAAACTCATTACCTATCCCCGCTCTGACAGCCGTTATTTACCCGTCGAGCAACATAGCCTAGCGCCGTCGGT
ACTTAAGGCGATTAGCGGTGGTGCCGCTGAGTTACTGCAAGGCGCCGATGCCCCCGATCCACGGCTAAAATCCAAGGCAT
GGGATGATAAAAAAGTGGATGCCCACCACGCCATCGTTCCTACCGAGAAAACGGCTAACTTATCCAGCTTAAGTCAGCGT
GAGAGGCAGTTATATCTTCATGTTGCCCGCCAGTACTTAGCCCAGTTTTATCCGGCCTATTGTTACAGTGAAACCACAGT
ACAAGTGACAATTGAGGGCGGCTTATTTAATACCAAGGCGCGTCAGGATAAATCTCTTGGCTGGAAGCAGCTCTTTGCCC
GCCAAGAGCCAAATGGCAGTAAGGCGAGTGGCAACAAGTCGACGGAGGAAAATGCGGGTAAGGATGATGAGGAGAATGAC
GAGTTTATCGGCCAATTACCGCCTCTGAAATTAGGGCTGGCTTTGCATTGTACTCGAGGCGAGTTACTTGAGAAAAATAC
TCAGCCGCCAAAGGCCTTTACCGATGCCACTTTACTTGGTGCTATGACGGGGATTAGCCGTTACGTAACCGACCCTGAGA
TCCGTAAAATTCTTAAAGAAACCGATGGCTTAGGTACTGAAGCCACCCGTGCAGGCATTATTGAACTACTGTTTAAACGC
GGTTTTTTACAACGCTTAGGTAAATCTATCGTTTCGACGGATGTGGGTAAGGGATTAATTAACAGCTTACCCGCGAGTGC
AACAACGCCGGACATGACAGCCCTTTGGGAAGCAAGCTTGAACGGTATTTGTCATAAAGAAACCTCCTATCAAGCCTTTA
TGCAACCGTTGCTAGGCACCTTGTCCACGCTTATCCAAAATGCAGGAGCACAACTGCCCACCGCGCTTAATGGTTTAAAA
GGCCAAGGTTATCGTAAAACGGCTGGGAATAAATCTGGTTATCGCAAGTCGCCTTACCGTAAAGCCTCTAGCTCTGCAAA
ACGCACTGGCACGGCAAGTACCGCTAAAACAAGTACAACGACAAGTAGCAGTGCGAAAAAGTCGAGTGCGAGTTCAGGGA
CTCGGAGTCGAACACGCAAAGTGGCTGCTGAGGTTTGA

Upstream 100 bases:

>100_bases
TTGAGCTTTGGTATTTAAGCTCGGTTTGTCACAGAAATTTGCACTCATCGGGCGTTTGGGCTAAATTGTACTGTAATTAT
ATCCAGTGTTTGGTGTTTGT

Downstream 100 bases:

>100_bases
TAGGCAAGAGTGTCATTGGTTTTAGGGGCATTTCCGACCATTAAACAATAGCTGGAAATAGCCCTAAATTTTGGCAAAAA
AAAGAGTAAACCAAAACGGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPDKWQLKPKAA
TRSQLTVLKRLVKQANTLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDEEIANFQSKPFYEVLAHLATEKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHSLAPSVLKAISGGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHVARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEENAGKDDEEND
EFIGQLPPLKLGLALHCTRGELLEKNTQPPKAFTDATLLGAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPASATTPDMTALWEASLNGICHKETSYQAFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTAGNKSGYRKSPYRKASSSAKRTGTASTAKTSTTTSSSAKKSSASSGTRSRTRKVAAEV

Sequences:

>Translated_705_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPDKWQLKPKAA
TRSQLTVLKRLVKQANTLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDEEIANFQSKPFYEVLAHLATEKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHSLAPSVLKAISGGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHVARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEENAGKDDEEND
EFIGQLPPLKLGLALHCTRGELLEKNTQPPKAFTDATLLGAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPASATTPDMTALWEASLNGICHKETSYQAFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTAGNKSGYRKSPYRKASSSAKRTGTASTAKTSTTTSSSAKKSSASSGTRSRTRKVAAEV
>Mature_705_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKSWKFEHLPIVPDKWQLKPKAA
TRSQLTVLKRLVKQANTLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDEEIANFQSKPFYEVLAHLATEKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQNPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHSLAPSVLKAISGGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHVARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEENAGKDDEEND
EFIGQLPPLKLGLALHCTRGELLEKNTQPPKAFTDATLLGAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPASATTPDMTALWEASLNGICHKETSYQAFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTAGNKSGYRKSPYRKASSSAKRTGTASTAKTSTTTSSSAKKSSASSGTRSRTRKVAAEV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=652, Percent_Identity=23.0061349693252, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1788061, Length=675, Percent_Identity=55.8518518518519, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=573, Percent_Identity=23.7347294938918, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585251, Length=538, Percent_Identity=24.7211895910781, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77353; Mature: 77353

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDKWQLKPKAATRSQLTVLKRLVKQANTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHC
VTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLVVRRDEEIANFQSKPFYEVLAHLATEKQETFSAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQNPPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPVEQHSLAPSVLKAISGGAAELLQ
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCHHHHHHHCCCHHHHHC
GADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQRERQLYLHVARQYLAQFYPAY
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEENAGKDDEEND
EECCCEEEEEEECCEECCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EFIGQLPPLKLGLALHCTRGELLEKNTQPPKAFTDATLLGAMTGISRYVTDPEIRKILKE
HHHHCCCCEEECEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TDGLGTEATRAGIIELLFKRGFLQRLGKSIVSTDVGKGLINSLPASATTPDMTALWEASL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NGICHKETSYQAFMQPLLGTLSTLIQNAGAQLPTALNGLKGQGYRKTAGNKSGYRKSPYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCH
KASSSAKRTGTASTAKTSTTTSSSAKKSSASSGTRSRTRKVAAEV
HHHCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKS
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDKWQLKPKAATRSQLTVLKRLVKQANTLVNAGDPDREGQLLVDEVIAYLG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHC
VTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLVVRRDEEIANFQSKPFYEVLAHLATEKQETFSAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQNPPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPVEQHSLAPSVLKAISGGAAELLQ
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCHHHHHHHCCCHHHHHC
GADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQRERQLYLHVARQYLAQFYPAY
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEENAGKDDEEND
EECCCEEEEEEECCEECCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EFIGQLPPLKLGLALHCTRGELLEKNTQPPKAFTDATLLGAMTGISRYVTDPEIRKILKE
HHHHCCCCEEECEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TDGLGTEATRAGIIELLFKRGFLQRLGKSIVSTDVGKGLINSLPASATTPDMTALWEASL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NGICHKETSYQAFMQPLLGTLSTLIQNAGAQLPTALNGLKGQGYRKTAGNKSGYRKSPYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCH
KASSSAKRTGTASTAKTSTTTSSSAKKSSASSGTRSRTRKVAAEV
HHHCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]