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The map label for this gene is purF [H]

Identifier: 126174945

GI number: 126174945

Start: 3212597

End: 3214111

Strand: Reverse

Name: purF [H]

Synonym: Sbal_2738

Alternate gene names: 126174945

Gene position: 3214111-3212597 (Counterclockwise)

Preceding gene: 126174946

Following gene: 126174943

Centisome position: 62.69

GC content: 47.79

Gene sequence:

>1515_bases
ATGTGTGGTATCGTCGGAATAGTTGGCCAATCATCGGTTAATCAGACCATTTATGATGCACTGACAGTGTTGCAGCACAG
AGGTCAGGATGCGGCAGGTATTGTGACCATTGATCGTGGTGCTTTCAGACTGCGTAAAGCCAATGGTCTGGTCAAAGACG
TTTTTGAAGTCAAACACATGCAACGCCTACAAGGTAATGCAGGTATTGGCCATGTGCGTTATCCAACTGCGGGCAGTTCA
AGCGCATCTGAAGCGCAGCCTTTCTATGTTAACTCGCCATTTGGGATCTCTTTAGCCCACAACGGCAACTTAACCAATAC
AGTCGAATTAGCCGAAGGGCTGATCAAAAAACGTCGCCATGTGAACACCACTTCGGATTCTGAAGTGTTGCTTAACCTGT
TGGCCGACGAACTGCAAAAAACCACTAGCTTGACCCTGACGTCGGAAGAAGTGTTCGATGCGGTCGCTAACGTACACCAA
CAAGCTCGTGGTGCCTACGCGGTTGTGGCTATGATCATCGGCCAAGGCTTAGTCGCGTTCCGTGACCCATTTGGTATTCG
TCCTCTGGTGTTAGGTAAGCACGAAACGCCAACGGGTACTGAATATATGGTCGCTTCTGAAAGCGTGGCACTCGATGCCG
TCGGTTTTGAAGTGATGCGTGATGTGGCGCCAGGTGAAGCGATTTACGTGTCAATTGATGGTCAGCTGTATACCCGTCAA
TGTGCAGCAGAACCTAGCTACGCCCCTTGTATCTTCGAATTCGTTTATTTTGCCCGTCCAGATTCGACTATCGATAAAGT
GTCTGTTTATGCCAGTCGCGTAAACATGGGCGCTAAGCTTGGCGAGAAGATTAAGAAAGAATGGTACGATCACGACATCG
ACGTGGTGATCCCTATTCCTGAAACTTCTTGCGATATCGCGCTGGAAATTGCCCGTTGCATGGACTTACCGTACCGCCAA
GGTTTTGTGAAAAACCGTTATATTGGCCGTACTTTTATCATGCCGGGTCAACAGGAACGTAAGAAGTCAGTGCGTCGCAA
GTTGAATGCGATTAACACTGAATTCAAAGGCAAAAATGTCTTGCTGGTGGACGACTCTATCGTTCGCGGTACGACGTCTG
AGCAAATCATCGAAATGGCCCGTGAAGCGGGTGCCAAGAAAGTGTACTTTGCCTCTGCCGCGCCGGAAATTCGTTTCCCG
AACGTGTACGGTATCGACATGCCAACGTCAAACGAGTTGATCGCCCATGGCCGTGATGCCAATGAAATCGCTAAGTTAAT
TGGTGCCGATGGCATTATTTTCCAAGACTTACCTGATTTAATCGAAGCGGTAAGAATGGAAAATCCAGCGATTAAGCGTT
TCGAAACCTCTGTTTTCGATGGTCATTACATCACTAACGATGTAGATCAAGCGTATTTAGATCACTTGACGCAACTGCGT
AATGACGATGCTAAAGCGGATCGTAACAAAGATATCGGTACTAACTTAGAGCTGCATAACGTCTGTCACCCATAA

Upstream 100 bases:

>100_bases
TAGGTAGCACTTAAGCCGAAAGGCTAGAGATTTACTTTATCATTTGCTTATATTTCAACATGAAAGGGCATAGAACATCT
TACAATGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
GGTGAAACGTTAGGCGGATAAAAAGCCAGTGATAATGTCATTGGCTTTTTTTATGGCTTTTTTATGGCTTTTTTATGGCT
TTTTTATAGAGCGTTAACCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTIDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDANEIAKLIGADGIIFQDLPDLIEAVRMENPAIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTIDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDANEIAKLIGADGIIFQDLPDLIEAVRMENPAIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP
>Mature_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTIDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDANEIAKLIGADGIIFQDLPDLIEAVRMENPAIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=469, Percent_Identity=35.8208955223881, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.558648111332, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=32.9479768786127, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=462, Percent_Identity=32.4675324675325, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=510, Percent_Identity=50.1960784313725, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=34.20523138833, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=34.8837209302326, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI28573187, Length=471, Percent_Identity=33.5456475583864, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55579; Mature: 55579

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTIDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHH
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHCCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
NEIAKLIGADGIIFQDLPDLIEAVRMENPAIKRFETSVFDGHYITNDVDQAYLDHLTQLR
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTIDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETPTGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHH
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHCCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
NEIAKLIGADGIIFQDLPDLIEAVRMENPAIKRFETSVFDGHYITNDVDQAYLDHLTQLR
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]