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The map label for this gene is rluB [H]

Identifier: 126174904

GI number: 126174904

Start: 3163150

End: 3164025

Strand: Reverse

Name: rluB [H]

Synonym: Sbal_2696

Alternate gene names: 126174904

Gene position: 3164025-3163150 (Counterclockwise)

Preceding gene: 126174905

Following gene: 126174903

Centisome position: 61.71

GC content: 51.26

Gene sequence:

>876_bases
ATGAGTGAAAAATTGCAGAAAGTCTTGGCCCGTGCAGGCCATGGCTCCCGTCGTGAGATGGAGGCATGGATTGCTGCAGG
CCGAGTTAGTATTGATGGTGAAATTGCTAGCCTTGGGGATCGGATTGAAGCCGATGCCAAAGTGCGTATCGATGGCAGAG
CCATTTCGTTAAAGTCTGCCGAAGACGTGATCTGTCGAGTATTGGCATACCATAAACCCGAAGGCGAAATTTGTAGTCGT
AAAGATCCTGAAGGCCGTCCAACGGTATTCGATCGCTTACCTAAAGTGCGTGATTCACGCTGGGTTGCGGTAGGTCGTTT
AGACATCAACACCTCAGGTTTATTGTTGTTTACCTCAGATGGTGAATTAGCGAACCGCTTAATGCATCCATCGAACGAAG
TCGACCGCGAATACGCAGTGCGTACCTTCGGTGAAATCCCTGAAGCGACAGTGCAACGTTTACGTACCGGCGTAATGTTA
GAAGACGGCCCTGCGCAGTTTGATAGCATCAAGCCAGCGGGCGGCGAAGGCATGAACCAGTGGTGGCATGTATGTTTACG
CGAAGGTCGTAACCGCGAAGTGCGCCGTCTGTGGGAATCCCAAGAAGTGCAAGTGAGCCGTTTGATCCGTATCCGTTACG
GTATGGTTGAATTGCCAAAATCACTGCCACGGGGTGGCTGGGTTGAATTACCAATGGAGCAAGTTAACTACTTGCGTAAA
TTGGCAGGTTTAGAGCCTGAAACCCGCACTATGCTTGCTGCCGATAAACACAGCGTAGCCCGTGCACAGGTGAAGAGTGC
CAAAATTCGCCGCGCCGTGCGTAAGCATAAAGTGACTGGATCGGGAAAACCTAAGCCATCGCGTCAGCGCAGCTAA

Upstream 100 bases:

>100_bases
ACCTTAGCGGATTTACCGCCGCTGTCGGATACTGAGTCATTGCAGGCGGTTTTTTCAAGTGTGAAATTAACGCCGGAACA
GTCAGAAGAGCAAAATACTA

Downstream 100 bases:

>100_bases
GTTGTTTTATTCCCATGAAAAAGGCCGCTTAGCGGCCTTTTTGCTTTCTGTTATTTGCAGCTATGTTATAACAAGAGTCA
GCTGGATTGAGGAGTGAATG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVML
EDGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPMEQVNYLRK
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGSGKPKPSRQRS

Sequences:

>Translated_291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVML
EDGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPMEQVNYLRK
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGSGKPKPSRQRS
>Mature_290_residues
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSAEDVICRVLAYHKPEGEICSRK
DPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLE
DGPAQFDSIKPAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPMEQVNYLRKL
AGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGSGKPKPSRQRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=293, Percent_Identity=63.8225255972696, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=29.4605809128631, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=29.0598290598291, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32803; Mature: 32672

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSA
CCHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHCCCCCCCCEEEECCEEEECCCH
EDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLEDGPAQFDSIKPAGGEGMNQ
CHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPMEQVNYLRK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGSGKPKPSRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGEIASLGDRIEADAKVRIDGRAISLKSA
CHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHCCCCCCCCEEEECCEEEECCCH
EDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEIPEATVQRLRTGVMLEDGPAQFDSIKPAGGEGMNQ
CHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRIRYGMVELPKSLPRGGWVELPMEQVNYLRK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTGSGKPKPSRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]