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The map label for this gene is ligA [H]

Identifier: 126174866

GI number: 126174866

Start: 3111031

End: 3111468

Strand: Reverse

Name: ligA [H]

Synonym: Sbal_2654

Alternate gene names: 126174866

Gene position: 3111468-3111031 (Counterclockwise)

Preceding gene: 126174867

Following gene: 126174865

Centisome position: 60.68

GC content: 47.95

Gene sequence:

>438_bases
ATGCAAGACATTCAACTCGATACACTTCTGTCTGAAGACGTTACCCCAGAAAACGCATCGCAGGTGATGCAGGCGCTCAG
CCAACCACTGAACGAGCACAACATTCGTTACTATGTGGATGATGCGCCGAGCATTACCGATTCCGAGTACGACAGGTTGA
TGCAGCGTTTACTCAAGCTTGAGGCGTTATATCCCCATTTGATTGTGGCTGATTCACCGACACAAAGGGTAGGCGGATTG
GCGCTCGCCAAGTTTGATCAGATCACTCATCTTAAGCCTATGTTAAGTCTCGATAACGCCTTCGATGAGGCTGATTTCAG
TGCTTTTCACAAACGTGTCACCGATAAAGTCGGTGAGGTGAGTTTTTGCTGCGAGCCTAAACTTGATGGCCTTGCCGTGA
GTATCTTGTATCGCAATGGGGTTTCCCTGATGTATTAA

Upstream 100 bases:

>100_bases
ACGCCGCAATTTGGCGGTTTATATATTGATGAGGCCGCTAATTGCGGCCTCATTTTTTGTGATCTACTTTTTGTGATCTA
AAGCATTCTGGATAAAGAAT

Downstream 100 bases:

>100_bases
AATTCACTTTTATGTTGTTAACGTTCGTCCAATTTATATGATGTAGTTATGGTCATTTTAGCAAGTGTTGTTTTAGATAG
ATTGATGTGATCCATACTTG

Product: DNA ligase (NAD(+))

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MQDIQLDTLLSEDVTPENASQVMQALSQPLNEHNIRYYVDDAPSITDSEYDRLMQRLLKLEALYPHLIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFDEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRNGVSLMY

Sequences:

>Translated_145_residues
MQDIQLDTLLSEDVTPENASQVMQALSQPLNEHNIRYYVDDAPSITDSEYDRLMQRLLKLEALYPHLIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFDEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRNGVSLMY
>Mature_145_residues
MQDIQLDTLLSEDVTPENASQVMQALSQPLNEHNIRYYVDDAPSITDSEYDRLMQRLLKLEALYPHLIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFDEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRNGVSLMY

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=128, Percent_Identity=57.03125, Blast_Score=142, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 16335; Mature: 16335

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIQLDTLLSEDVTPENASQVMQALSQPLNEHNIRYYVDDAPSITDSEYDRLMQRLLKL
CCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
EALYPHLIVADSPTQRVGGLALAKFDQITHLKPMLSLDNAFDEADFSAFHKRVTDKVGEV
HHHCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCE
SFCCEPKLDGLAVSILYRNGVSLMY
EEECCCCCCCEEEEEEEECCCEECC
>Mature Secondary Structure
MQDIQLDTLLSEDVTPENASQVMQALSQPLNEHNIRYYVDDAPSITDSEYDRLMQRLLKL
CCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
EALYPHLIVADSPTQRVGGLALAKFDQITHLKPMLSLDNAFDEADFSAFHKRVTDKVGEV
HHHCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCE
SFCCEPKLDGLAVSILYRNGVSLMY
EEECCCCCCCEEEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]