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The map label for this gene is ligA

Identifier: 126174865

GI number: 126174865

Start: 3108900

End: 3110846

Strand: Reverse

Name: ligA

Synonym: Sbal_2653

Alternate gene names: 126174865

Gene position: 3110846-3108900 (Counterclockwise)

Preceding gene: 126174866

Following gene: 126174858

Centisome position: 60.67

GC content: 37.39

Gene sequence:

>1947_bases
ATGAAAGAATTTAAGTTTAATGATGAGCAACTCTCAACAATACAAGAGCTAGAAGTAGATATTGATTTAAAAAAATTAGA
AATTGCTATTAGTAAAGAAAGCATTGATGAACTTAGCGAAGATGAATTGCTAGCTTTACTTAAGGTAACCAACGCTTTAT
ATCGTTCAGGTTTTCCAGTAATTGAAGATATCCTTTATGACTCTTTTTGGAATAAATTTGCTCTAAACCATCCAAATCAT
CCATTCATTACTTCGGTAGAAACTGAAGTCTTGAACCTTGGTAAAACAGTAGCTCTACCTCAAAAGATGTTGTCAACTGA
TAAGGCATATTCAAAAGAGGAAATTAAGAAGTGGTTAGATAGAATTCTAAAAGCTGCTGAAGAATTAGATATTGATACAA
ACGAAATCGAAATTAGGATTACTCCAAAGCTTGATGGTTATGCTGCTTTTGACGACGGAGAAAGACTCTATACACGTGGT
GACGGCGTAAAAGGTCAAGATGTATCAAGAGCTTTCGAACGTGGTTTAAAAGTAGTAAGAAATAGTGAGAGAGGCTTAGG
CGCTGGTGAGATAGTCATCAATAAAGCTTATTTTGAAAAAAAGCTTAGTCAGTACTTCGAAAATTCTCGAAACATTCAAG
CTGCTATCATTGCTGAGAAAAACGTTGATGAGAGAGTTCTTGAGGCAATAAAAGAAGGTGCTTGTGTTTTTCATCCATTT
GCTGTGCTTCCTAATTGGACGGGTAATTATCAGGATTTGATGACGGATTTTGATGGCATTATTGAGCAAGTATGGAATTC
TGTCGAGTATGACGTAGATGGTGTGATTTTGGAGGCCACAGACACTCGCATAAAGGAGCATATGGGAGCTAATAGAAAGT
TCCATCGTTGGCAAATAGCATTCAAGATTAATGATGAAGTTGCTGAAGTAGAAGTTATCAGTGTTACTCCACAAACTTCA
AGAACAGGTAGAGTAACACCAGTTGCTGAATTAGTGCCAACTAGAATTAGTGGCGCTATGATTAGCCGAGTTACAGTGCA
CCATTACAATATGGTTAAAATCAATGGTGTAGGCCCTGGCGCGATAGTTCAAATAGTTAGAAGTGGCTTGGTTATCCCTA
AAATAGAAAAAGTTATAAAGCCCGTAGACCCTCAAATGCCAGAATTATGCCCTAGTTGTAAGTCTCATCTTCTATGGGAA
TCTGATCACTTGGTATGTCCAAATAAGAGTGACTGTCCTGCACAGACAGAGAACACGCTTGTGCACTTTTTTAAAACACT
AGCTAATAATGACGGTTTTGGCCCTAAGAATATCGAAAAACTAGCAAATCTCGGAGTTAAGCATATCCATCAAATGTATG
ATTTGAAACCGCATCAGTTTGCGATGTATGGTTTTGGTGAGAAGACATCTAAGAACCTATATGATCAGCTACAAGCAAGT
AAAAATATTGAAATTGAAGACTGGCGTTTCCTTTCTGCATTTGGTGTGAGTCGTTTGGGGGGAGGTAATTGCGAGAAATT
GCTTCAGCATTACTCATTACGAGAACTCTTTGAAGCGACCGTAGAAGATATCGCCGAGTTAAATGGGTTCGCAAGAGTTA
GTGCAGAAGCCATTGTTAAAGGTCTAGCTAACATTAAAGAAGAATTTTTTAAAGTTTATGACTTGGGATTTAATTTAAGT
ATCACTCCTAAGCAGTCCGAGCTAGAAAGTTTAGCTTCACCGCTTACTGGTAAAATAGTTGTTTTCACTGGAAAAATGCT
TCAGGGAAAACGGAGTGACATGGAAAAGCATGCTAAAGCACTAGGGGCGAAAGTAGGTACATCTGTGACCAGTAATACAA
CCTATCTGATTGCTGGATTGAAAGTTGGTGAAACCAAAATCAATGATGCAAAAGAGAAAGGAGTGAAAGTTTTATCTGAG
CAGGAATATTTAGATTTAATTAGTTAA

Upstream 100 bases:

>100_bases
ATGTGATCCATACTTGCTAAGTTGAATCAGGCCTAGTTATTCACTCGTTAAGCAGAGTGCTGCTTCTAGGCCAATAACTA
AAAAAAATCGGTAATAAATA

Downstream 100 bases:

>100_bases
TTTTATTGATTTAAAAATTATAAAGGGACCGCTTTGTAAGCGGCCCCTTTTATGATGAAAATTTGCTCAGTGTTTAGATA
GTAGTTCATCAACTTCTATT

Product: DNA ligase (NAD(+))

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MKEFKFNDEQLSTIQELEVDIDLKKLEIAISKESIDELSEDELLALLKVTNALYRSGFPVIEDILYDSFWNKFALNHPNH
PFITSVETEVLNLGKTVALPQKMLSTDKAYSKEEIKKWLDRILKAAEELDIDTNEIEIRITPKLDGYAAFDDGERLYTRG
DGVKGQDVSRAFERGLKVVRNSERGLGAGEIVINKAYFEKKLSQYFENSRNIQAAIIAEKNVDERVLEAIKEGACVFHPF
AVLPNWTGNYQDLMTDFDGIIEQVWNSVEYDVDGVILEATDTRIKEHMGANRKFHRWQIAFKINDEVAEVEVISVTPQTS
RTGRVTPVAELVPTRISGAMISRVTVHHYNMVKINGVGPGAIVQIVRSGLVIPKIEKVIKPVDPQMPELCPSCKSHLLWE
SDHLVCPNKSDCPAQTENTLVHFFKTLANNDGFGPKNIEKLANLGVKHIHQMYDLKPHQFAMYGFGEKTSKNLYDQLQAS
KNIEIEDWRFLSAFGVSRLGGGNCEKLLQHYSLRELFEATVEDIAELNGFARVSAEAIVKGLANIKEEFFKVYDLGFNLS
ITPKQSELESLASPLTGKIVVFTGKMLQGKRSDMEKHAKALGAKVGTSVTSNTTYLIAGLKVGETKINDAKEKGVKVLSE
QEYLDLIS

Sequences:

>Translated_648_residues
MKEFKFNDEQLSTIQELEVDIDLKKLEIAISKESIDELSEDELLALLKVTNALYRSGFPVIEDILYDSFWNKFALNHPNH
PFITSVETEVLNLGKTVALPQKMLSTDKAYSKEEIKKWLDRILKAAEELDIDTNEIEIRITPKLDGYAAFDDGERLYTRG
DGVKGQDVSRAFERGLKVVRNSERGLGAGEIVINKAYFEKKLSQYFENSRNIQAAIIAEKNVDERVLEAIKEGACVFHPF
AVLPNWTGNYQDLMTDFDGIIEQVWNSVEYDVDGVILEATDTRIKEHMGANRKFHRWQIAFKINDEVAEVEVISVTPQTS
RTGRVTPVAELVPTRISGAMISRVTVHHYNMVKINGVGPGAIVQIVRSGLVIPKIEKVIKPVDPQMPELCPSCKSHLLWE
SDHLVCPNKSDCPAQTENTLVHFFKTLANNDGFGPKNIEKLANLGVKHIHQMYDLKPHQFAMYGFGEKTSKNLYDQLQAS
KNIEIEDWRFLSAFGVSRLGGGNCEKLLQHYSLRELFEATVEDIAELNGFARVSAEAIVKGLANIKEEFFKVYDLGFNLS
ITPKQSELESLASPLTGKIVVFTGKMLQGKRSDMEKHAKALGAKVGTSVTSNTTYLIAGLKVGETKINDAKEKGVKVLSE
QEYLDLIS
>Mature_648_residues
MKEFKFNDEQLSTIQELEVDIDLKKLEIAISKESIDELSEDELLALLKVTNALYRSGFPVIEDILYDSFWNKFALNHPNH
PFITSVETEVLNLGKTVALPQKMLSTDKAYSKEEIKKWLDRILKAAEELDIDTNEIEIRITPKLDGYAAFDDGERLYTRG
DGVKGQDVSRAFERGLKVVRNSERGLGAGEIVINKAYFEKKLSQYFENSRNIQAAIIAEKNVDERVLEAIKEGACVFHPF
AVLPNWTGNYQDLMTDFDGIIEQVWNSVEYDVDGVILEATDTRIKEHMGANRKFHRWQIAFKINDEVAEVEVISVTPQTS
RTGRVTPVAELVPTRISGAMISRVTVHHYNMVKINGVGPGAIVQIVRSGLVIPKIEKVIKPVDPQMPELCPSCKSHLLWE
SDHLVCPNKSDCPAQTENTLVHFFKTLANNDGFGPKNIEKLANLGVKHIHQMYDLKPHQFAMYGFGEKTSKNLYDQLQAS
KNIEIEDWRFLSAFGVSRLGGGNCEKLLQHYSLRELFEATVEDIAELNGFARVSAEAIVKGLANIKEEFFKVYDLGFNLS
ITPKQSELESLASPLTGKIVVFTGKMLQGKRSDMEKHAKALGAKVGTSVTSNTTYLIAGLKVGETKINDAKEKGVKVLSE
QEYLDLIS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=660, Percent_Identity=26.969696969697, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SHEB5 (A3D5Y2)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051014.1
- ProteinModelPortal:   A3D5Y2
- STRING:   A3D5Y2
- GeneID:   4843621
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_2653
- NMPDR:   fig|325240.3.peg.1600
- eggNOG:   COG0272
- HOGENOM:   HBG295055
- OMA:   EDWRFLA
- ProtClustDB:   CLSK831577
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 72734; Mature: 72734

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 143-143 BINDING 159-159 BINDING 190-190 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFKFNDEQLSTIQELEVDIDLKKLEIAISKESIDELSEDELLALLKVTNALYRSGFPV
CCCCCCCHHHHHHHHHHHHCCCHHHEEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
IEDILYDSFWNKFALNHPNHPFITSVETEVLNLGKTVALPQKMLSTDKAYSKEEIKKWLD
HHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHCCCHHHHHHHHHHCHHCCCHHHHHHHHH
RILKAAEELDIDTNEIEIRITPKLDGYAAFDDGERLYTRGDGVKGQDVSRAFERGLKVVR
HHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
NSERGLGAGEIVINKAYFEKKLSQYFENSRNIQAAIIAEKNVDERVLEAIKEGACVFHPF
CCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECH
AVLPNWTGNYQDLMTDFDGIIEQVWNSVEYDVDGVILEATDTRIKEHMGANRKFHRWQIA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEE
FKINDEVAEVEVISVTPQTSRTGRVTPVAELVPTRISGAMISRVTVHHYNMVKINGVGPG
EEECCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHH
AIVQIVRSGLVIPKIEKVIKPVDPQMPELCPSCKSHLLWESDHLVCPNKSDCPAQTENTL
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHCHHHHHHCEECCCCEECCCCCCCCCCHHHHH
VHFFKTLANNDGFGPKNIEKLANLGVKHIHQMYDLKPHQFAMYGFGEKTSKNLYDQLQAS
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHC
KNIEIEDWRFLSAFGVSRLGGGNCEKLLQHYSLRELFEATVEDIAELNGFARVSAEAIVK
CCCCCHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GLANIKEEFFKVYDLGFNLSITPKQSELESLASPLTGKIVVFTGKMLQGKRSDMEKHAKA
HHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCEEEEEECHHHCCCHHHHHHHHHH
LGAKVGTSVTSNTTYLIAGLKVGETKINDAKEKGVKVLSEQEYLDLIS
HHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEFKFNDEQLSTIQELEVDIDLKKLEIAISKESIDELSEDELLALLKVTNALYRSGFPV
CCCCCCCHHHHHHHHHHHHCCCHHHEEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
IEDILYDSFWNKFALNHPNHPFITSVETEVLNLGKTVALPQKMLSTDKAYSKEEIKKWLD
HHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHCCCHHHHHHHHHHCHHCCCHHHHHHHHH
RILKAAEELDIDTNEIEIRITPKLDGYAAFDDGERLYTRGDGVKGQDVSRAFERGLKVVR
HHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
NSERGLGAGEIVINKAYFEKKLSQYFENSRNIQAAIIAEKNVDERVLEAIKEGACVFHPF
CCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECH
AVLPNWTGNYQDLMTDFDGIIEQVWNSVEYDVDGVILEATDTRIKEHMGANRKFHRWQIA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEE
FKINDEVAEVEVISVTPQTSRTGRVTPVAELVPTRISGAMISRVTVHHYNMVKINGVGPG
EEECCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHH
AIVQIVRSGLVIPKIEKVIKPVDPQMPELCPSCKSHLLWESDHLVCPNKSDCPAQTENTL
HHHHHHHCCCCCHHHHHHHCCCCCCCHHHCHHHHHHCEECCCCEECCCCCCCCCCHHHHH
VHFFKTLANNDGFGPKNIEKLANLGVKHIHQMYDLKPHQFAMYGFGEKTSKNLYDQLQAS
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHC
KNIEIEDWRFLSAFGVSRLGGGNCEKLLQHYSLRELFEATVEDIAELNGFARVSAEAIVK
CCCCCHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GLANIKEEFFKVYDLGFNLSITPKQSELESLASPLTGKIVVFTGKMLQGKRSDMEKHAKA
HHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCEEEEEECHHHCCCHHHHHHHHHH
LGAKVGTSVTSNTTYLIAGLKVGETKINDAKEKGVKVLSEQEYLDLIS
HHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA