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The map label for this gene is insI1 [C]

Identifier: 126174858

GI number: 126174858

Start: 3103017

End: 3103772

Strand: Reverse

Name: insI1 [C]

Synonym: Sbal_2645

Alternate gene names: 126174858

Gene position: 3103772-3103017 (Counterclockwise)

Preceding gene: 126174865

Following gene: 126174856

Centisome position: 60.53

GC content: 46.3

Gene sequence:

>756_bases
ATGAGGCGTCAGTCGAGTAAATATGCGATACCCTCACAGCGAATAGAGAGCATCATCCTTTTGCTGCAATTGGATTGGAG
CCCGGAGCAAATTTCGCAGGTGTTGCGTCTGGTGAAGGAGCCCGTCAGTCACGAGTGGATTTATCGCTATATTGCACGCG
ATAAACGCCGTGGTGGCAAACTTTATCGTCATTTACGTCAAGGACATAAGCGTTATCGGCGCGGTAAAGTAGAGCAAGCT
CCTACCATAAAAAACGCCGTATCCATTGACGACAGACCAGCCATTGTTGATAGCCGAGAGCGATTTGGTGACTGGGAAAT
CGACACCGTATTGGGGCTGCATGGAACGGGTTCGATTGTAACCCTATTAGAGCGTAAGACTCGGTTTTATTTGATAAAGA
AAGTCAATTCGAAGTCAGCGGCAGATGTAACGAAAGCGACGATTGAGTTACTGATGCCCTATAAGGCGCATGTGCTGACA
ATCACGGCAGATAATGGACGCGAATTTGCTCACCACCAAGAGATAGCAGAGGCGCTAGATACACAGGTGTATTTTGCGCA
CCCTTATCGTTCTTGTGAACGAGGAGCGAATGAAAACGCCAATGGATTACTGCGGCAGTACGTCAAAAAAGGGGTTGATT
TGAGGCTAGTGAGTGATGAATTAATCGAATTTGCTCAGAATAGAATTAACTATCGACCCAAAAAGTGTTTGAAGTTTAAG
CAGCCTGCGGTGGTATTTCACCAATTAGCTGCTTAA

Upstream 100 bases:

>100_bases
ACCGGTCAGGGTAATTGTCGCGCAATACTATAGGGAAGTTAAACGATGCCAAGGGCAATCACGCTATCAGCCTGATATTG
CACACAAGCAGGCTGTCACA

Downstream 100 bases:

>100_bases
TTGTCGAGTGTCGCACTTCGGAGTTGAATTCGGGGTTTAAGGAAATCATTTTCAATTTTTTCTCTAATATTTTCGTTTGT
GAGCTTGGTATTTGATTTCT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQA
PTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLT
ITADNGREFAHHQEIAEALDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFK
QPAVVFHQLAA

Sequences:

>Translated_251_residues
MRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQA
PTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLT
ITADNGREFAHHQEIAEALDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFK
QPAVVFHQLAA
>Mature_251_residues
MRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQA
PTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLT
ITADNGREFAHHQEIAEALDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFK
QPAVVFHQLAA

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=245, Percent_Identity=32.2448979591837, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787671, Length=245, Percent_Identity=32.2448979591837, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786450, Length=245, Percent_Identity=32.2448979591837, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29119; Mature: 29119

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGK
CCCCCCCCCCCHHHHHHEEEHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHH
LYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGSIV
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCHHHEEECCCCCHHH
TLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEALD
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHC
TQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFK
CCEEEECCHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHCC
QPAVVFHQLAA
CHHHHHHHHCC
>Mature Secondary Structure
MRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGK
CCCCCCCCCCCHHHHHHEEEHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHH
LYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGSIV
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCHHHEEECCCCCHHH
TLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEALD
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHC
TQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFK
CCEEEECCHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHCC
QPAVVFHQLAA
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]